LOCUS       sequence-335489-       10453 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes SARS-CoV-2 B.1.351v1/wtRBD Spike Protein lacking 18 
            C-terminal amino acids for pseudovirus production.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10453)
  AUTHORS   Garcia-Beltran WF, Lam EC, St Denis K, Nitido AD, Garcia ZH, Hauser 
            BM, Feldman J, Pavlovic MN, Gregory DJ, Poznansky MC, Sigal A, 
            Schmidt AG, Iafrate AJ, Naranbhai V, Balazs AB
  TITLE     Multiple SARS-CoV-2 variants escape neutralization by 
            vaccine-induced humoral immunity.
  JOURNAL   Cell. 2021 Mar 12. pii: S0092-8674(21)00298-1. doi: 
            10.1016/j.cell.2021.03.013.
  PUBMED    33743213
REFERENCE   2  (bases 1 to 10453)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2021 
            Mar 12. pii: S0092-8674(21)00298-1. doi: 
            10.1016/j.cell.2021.03.013."
COMMENT     Sequence Label: pTwist-SARS-CoV-2 Delta-18 B.1.351v1/wtRBD
FEATURES             Location/Qualifiers
     source          1..10453
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    3797..4385
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3850..3870)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4268..4279)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    4438..4559
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4449..4468)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4503..4522
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     terminator      4674..4720
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      4750..4886
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    4931..5002
                     /label=3' beta-globin insulator
                     /note="insulator downstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     promoter        5047..5243
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      5094..5229
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5257..6051
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5311..5330)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5921..5940
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6062..6286
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(6482..7070)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6562..6581)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             join(7816..7884,7885..8676)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8034..8053)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     terminator      8689..8825
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    8977..9048
                     /label=5' beta-globin insulator
                     /note="insulator upstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     terminator      complement(9053..9099)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     polyA_signal    9117..9165
                     /note="synthetic polyadenylation signal"
     enhancer        9246..9549
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9551..9753
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     9704..9724
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     intron          9887..10362
                     /label=beta-globin intron
                     /note="internally truncated intron from human beta-globin"
ORIGIN
        1 tcttcccaat gcgtgaacct cacaactaga acgcagctcc cacccgctta cacgaactct
       61 tttactcgtg gagtatacta tcccgataaa gtctttaggt ccagtgtcct tcattctact
      121 caagaccttt tcctgccatt cttctcaaat gtcacctggt tccatgctat ccacgtttct
      181 ggcaccaacg gaactaaacg atttgctaat cccgtcttgc catttaacga tggcgtatac
      241 tttgcctcaa cggagaagtc caacattatc cggggatgga tatttggcac caccctggac
      301 tccaagacac agtctctcct cattgtgaac aacgccacca atgtcgtcat caaagtctgt
      361 gaatttcaat tttgtaacga tccctttctc ggggtttatt atcataagaa taacaagtct
      421 tggatggagt cagagttccg cgtttacagt tccgctaaca actgtacttt tgagtacgtt
      481 tcccaaccat ttctcatgga tcttgaaggg aagcaaggca acttcaagaa tctccgcgaa
      541 tttgtgttta agaatataga cggctacttc aaaatttata gcaagcatac cccgataaat
      601 ctggttcggg gtctccctca aggcttctcc gcgttggaac ccctcgtgga cttgccgatt
      661 ggcatcaaca tcacccggtt ccaaaccctt catcgaagct acctcacgcc tggcgacagt
      721 agtagtggtt ggacagcggg cgcagcagcc tactatgtgg gatatttgca accacgcacc
      781 tttcttctca aatataacga aaacggaaca attaccgatg ccgtagactg tgccctcgat
      841 cccctgtccg aaactaaatg cacactcaag tccttcacag ttgagaaagg catataccaa
      901 accagtaact tccgcgtcca acccaccgaa tccattgttc gattccctaa catcaccaat
      961 ttgtgtcctt tcggtgaagt atttaatgcc actcgatttg catcagttta tgcctggaat
     1021 cgcaagcgaa tatcaaattg tgttgcagat tatagtgtcc tgtataatag cgcatcattc
     1081 agcactttta agtgttatgg ggtcagccct acgaaattga atgacttgtg ttttaccaac
     1141 gtttacgcag attctttcgt catacgcggc gacgaagtcc gacagattgc accggggcaa
     1201 accggaaaga tagcagacta taattataag ttgccggatg acttcaccgg ctgcgttata
     1261 gcatggaact ccaataatct cgattcaaaa gtcggcggca actataatta tctttaccgc
     1321 ctgtttcgca agtcaaatct gaagcccttt gaacgtgaca tctccaccga aatttatcaa
     1381 gctggcagca ccccatgtaa tggagtggaa gggtttaact gttattttcc ccttcaatca
     1441 tacggatttc aaccaacaaa tggggtgggc tatcaaccat atcgggtggt agtcttgagc
     1501 tttgagctgc tccacgcacc ggctacagtt tgcggaccga agaaaagcac taacctggtg
     1561 aagaataaat gtgtgaactt caactttaat gggctcaccg gaacaggggt gctgacagaa
     1621 tcaaacaaga aatttctccc ttttcaacaa tttggtagag acattgccga taccacagat
     1681 gccgtccgcg atccgcaaac actggaaatc ctcgatataa caccctgtag cttcggcggg
     1741 gtcagcgtga taactcccgg aacgaacaca tccaaccaag tggctgtcct gtatcaaggt
     1801 gtcaactgta ccgaagtccc tgtggcgata cacgccgacc aactgacacc cacctggaga
     1861 gtctactcaa caggttccaa tgtctttcaa actagagcgg gttgtctcat tggcgctgag
     1921 catgtcaata attcatacga atgtgatatt cctattgggg caggaatctg tgcgtcttat
     1981 caaactcaaa cgaactcacc acggcgcgct cgctcagtag ccagccagtc cataatcgcc
     2041 tataccatga gtctcggggt tgagaactcc gtggcttatt caaacaattc catcgcaatc
     2101 ccaactaact ttaccattag cgttaccacg gaaatcttgc ccgtcagcat gactaagacg
     2161 agcgtagact gtacgatgta tatctgtggg gattccaccg agtgtagcaa cctcttgctt
     2221 caatacgggt ctttctgtac tcaactcaac cgagcattga ccggaattgc ggtcgaacaa
     2281 gacaagaata cccaagaggt gttcgcacaa gtaaagcaaa tatacaaaac gccgccaatc
     2341 aaagattttg gcgggtttaa ttttagccaa atactgccag acccatctaa gccttcaaaa
     2401 cgcagcttta tcgaggatct gctgttcaac aaagttacgt tggctgacgc gggtttcatt
     2461 aagcagtatg gggattgttt gggagacatt gcggcccgcg accttatctg tgctcaaaaa
     2521 ttcaatgggt tgacagttct gccccctctg ctcactgatg agatgattgc acagtataca
     2581 tcagcattgt tggcaggaac cattacatcc ggctggactt ttggcgcggg tgcggccctt
     2641 caaatacctt tcgctatgca aatggcttac agattcaatg ggattggtgt cacgcaaaat
     2701 gtattgtatg aaaatcagaa gctgatcgct aatcaattta acagcgcgat tggcaagatc
     2761 caagattcac tgtcaagcac agcgtccgcc ctcggcaaac tccaagatgt tgtgaatcaa
     2821 aacgcccagg ccctgaatac cctcgtgaag caactgagtt ctaacttcgg cgcaatctcc
     2881 agcgtcctca acgacatatt gtccagactt gataaggtgg aagccgaggt gcaaattgat
     2941 cggctcatca caggcaggct ccagtcactc caaacttatg tgacacaaca gttgatccgg
     3001 gctgctgaga tacgagcaag cgccaacctg gccgccacta aaatgtcaga gtgcgtgctc
     3061 gggcagtcca aacgggttga cttttgtggc aagggctatc acttgatgtc cttcccccaa
     3121 tccgccccac atggagtggt ctttctccac gtcacttatg tccctgctca agagaagaac
     3181 tttacgacag ctccagcaat ctgtcacgat ggcaaagcgc actttcccag ggaaggcgtt
     3241 ttcgtcagta atggcacgca ttggtttgtg acccaaagga atttctatga accccaaatc
     3301 ataaccactg acaatacatt tgtctcaggc aactgtgatg tggtcattgg gatcgtgaac
     3361 aacacggtat atgatcccct gcaacccgag ttggatagct tcaaagagga attggataaa
     3421 tactttaaga atcatacttc acccgatgtt gatctcgggg acatatcagg aataaatgca
     3481 agtgtcgtca acattcagaa agagattgat cgccttaatg aggtcgccaa gaacttgaat
     3541 gaatctttga tcgacttgca agaactgggc aaatacgaac aatatattaa atggccctgg
     3601 tacatttggt tgggctttat cgccggactt atagctatcg ttatggtcac aattatgttg
     3661 tgctgtatga ctagctgttg ttcctgcttg aaaggatgtt gcagttgcgg atcttgttgt
     3721 aaataaagga tcctctagaa gttgtctcct cctgcactga ctgactgata caatcgattt
     3781 ctggatccgc aggcctaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat
     3841 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca
     3901 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc
     3961 tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc
     4021 tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt
     4081 cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg
     4141 gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc
     4201 ctttccatgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta
     4261 cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg
     4321 gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc
     4381 cccgcctgtc tagcttgact gactgagata cagcgtacct tcagctcaca gacatgataa
     4441 gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt
     4501 gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta
     4561 acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt
     4621 aaagcaagta aaacctctac aaatgtggta ttggcccatc tctatcggta tcgtagcata
     4681 accccttggg gcctctaaac gggtcttgag gggttttttg tgcccctcgg gccggattgc
     4741 tatctaccgg cattggcgca gaaaaaaatg cctgatgcga cgctgcgcgt cttatactcc
     4801 cacatatgcc agattcagca acggatacgg cttccccaac ttgcccactt ccatacgtgt
     4861 cctccttacc agaaatttat ccttaaggtc gtcagctatc ctgcaggcga tctctcgatt
     4921 tcgatcaaga cattccttta atggtctttt ctggacacca ctaggggtca gaagtagttc
     4981 atcaaacttt cttccctccc taatctcatt ggttaccttg ggctatcgaa acttaattaa
     5041 gcgatctgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc
     5101 ccctaactcc gcccagttcc gcccattctc cgccccatcg ctgactaatt ttttttattt
     5161 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt
     5221 ttggaggcct aggcttttgc aaaggaggta gccaacatga ttgaacaaga tggattgcac
     5281 gcaggttctc ccgccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca
     5341 atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt
     5401 gtcaagaccg acctgtccgg tgccctgaat gaactccagg acgaggcagc gcggctatcg
     5461 tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga
     5521 agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct
     5581 cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg
     5641 gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg
     5701 gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc
     5761 gaactgttcg ccaggctcaa ggcgcggatg cccgacggcg aggatctcgt cgtgacccac
     5821 ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac
     5881 tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt
     5941 gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct
     6001 cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttcta gtatgtaagc
     6061 cctgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc
     6121 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt
     6181 ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat
     6241 tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggttaa ttaaccagtc
     6301 aagtcagcta cttggcgaga tcgacttgtc tgggtttcga ctacgctcag aattgcgtca
     6361 gtcaagttcg atctggtcct tgctattgca cccgttctcc gattacgagt ttcatttaaa
     6421 tcatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     6481 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     6541 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     6601 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     6661 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     6721 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     6781 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     6841 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     6901 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     6961 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     7021 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     7081 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     7141 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     7201 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     7261 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcattt aaatttccga
     7321 actctccaag gccctcgtcg gaaaatcttc aaacctttcg tccgatccat cttgcaggct
     7381 acctctcgaa cgaactatcg caagtctctt ggccggcctt gcgccttggc tattgcttgg
     7441 cagcgcctat cgccaggtat tactccaatc ccgaatatcc gagatcggga tcacccgaga
     7501 gaagttcaac ctacatcctc aatcccgatc tatccgagat ccgaggaata tcgaaatcgg
     7561 ggcgcgcctg gtgtaccgag aacgatcctc tcagtgcgag tctcgacgat ccatatcgtt
     7621 gcttggcagt cagccagtcg gaatccagct tgggacccag gaagtccaat cgtcagatat
     7681 tgtactcaag cctggtcacg gcagcgtacc gatctgttta aacctagata ttgatagtct
     7741 gatcggtcaa cgtataatcg agtcctagct tttgcaaaca tctatcaaga gacaggatca
     7801 gcaggaggct ttcgcatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     7861 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     7921 gatcagttgg gtgcgcgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     7981 gagagttttc gccccgaaga acgctttcca atgatgagca cttttaaagt tctgctatgt
     8041 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
     8101 tctcagaatg acttggttga gtattcacca gtcacagaaa agcatcttac ggatggcatg
     8161 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     8221 cttctgacaa cgattggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     8281 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     8341 cgtgacacca cgatgcctgt agcaatggca acaaccttgc gtaaactatt aactggcgaa
     8401 ctacttactc tagcttcccg gcaacagttg atagactgga tggaggcgga taaagttgca
     8461 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     8521 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     8581 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     8641 gctgagatag gtgcctcact gattaagcat tggtaaccga ttctaggtgc attggcgcag
     8701 aaaaaaatgc ctgatgcgac gctgcgcgtc ttatactccc acatatgcca gattcagcaa
     8761 cggatacggc ttccccaact tgcccacttc catacgtgtc ctccttacca gaaatttatc
     8821 cttaagatcc cgaatcgttt aaactcgact ctggctctat cgaatctccg tcgtttcgag
     8881 cttacgcgaa cagccgtggc gctcatttgc tcgtcgggca tcgaatctcg tcagctatcg
     8941 tcagcttacc tttttggcag cgatcgcggc tcccgacatc ttggaccatt agctccacag
     9001 gtatcttctt ccctctagtg gtcataacag cagcttcagc tacctctcaa ttcaaaaaac
     9061 ccctcaagac ccgtttagag gccccaaggg gttatgctat caatcgttgc gttacacaca
     9121 caaaaaacca acacacatcc atcttcgatg gatagcgatt ttattatcta actgctgatc
     9181 gagtgtagcc agatctagta atcaattacg gggtcattag ttcatagccc atatatggag
     9241 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
     9301 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
     9361 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
     9421 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
     9481 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
     9541 attaccatgc tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
     9601 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
     9661 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
     9721 cgtgtacggt gggaggtcta tataagcaga gctggtttag tgaaccgtca gatcgcctgg
     9781 agacgccatc cacgctgttt tgacctccat agaagacacc gggaccgatc cagcctcccc
     9841 tcgaagctta catgtggtac cgagctcgga tcctgagaac ttcagggtga gtctatggga
     9901 cccttgatgt tttctttccc cttcttttct atggttaagt tcatgtcata ggaaggggag
     9961 aagtaacagg gtacacatat tgaccaaatc agggtaattt tgcatttgta attttaaaaa
    10021 atgctttctt cttttaatat acttttttgt ttatcttatt tctaatactt tccctaatct
    10081 ctttctttca gggcaataat gatacaatgt atcatgcctc tttgcaccat tctaaagaat
    10141 aacagtgata atttctgggt taaggcaata gcaatatttc tgcatataaa tatttctgca
    10201 tataaattgt aactgatgta agaggtttca tattgctaat agcagctaca atccagctac
    10261 cattctgctt ttattttatg gttgggataa ggctggatta ttctgagtcc aagctaggcc
    10321 cttttgctaa tcatgttcat acctcttatc ttcctcccac agctcctggg caacgtgctg
    10381 gtctgtgtgc tggcccatca ctttggcaaa ggcggccgcc atgtttgtgt tcctcgttct
    10441 cctgcccctc gtt
//
