LOCUS       sequence-335490-       10453 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes SARS-CoV-2 B.1.351v3/wtRBD Spike Protein lacking 18 
            C-terminal amino acids for pseudovirus production.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10453)
  AUTHORS   Garcia-Beltran WF, Lam EC, St Denis K, Nitido AD, Garcia ZH, Hauser 
            BM, Feldman J, Pavlovic MN, Gregory DJ, Poznansky MC, Sigal A, 
            Schmidt AG, Iafrate AJ, Naranbhai V, Balazs AB
  TITLE     Multiple SARS-CoV-2 variants escape neutralization by 
            vaccine-induced humoral immunity.
  JOURNAL   Cell. 2021 Mar 12. pii: S0092-8674(21)00298-1. doi: 
            10.1016/j.cell.2021.03.013.
  PUBMED    33743213
REFERENCE   2  (bases 1 to 10453)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2021 
            Mar 12. pii: S0092-8674(21)00298-1. doi: 
            10.1016/j.cell.2021.03.013."
COMMENT     Sequence Label: pTwist-SARS-CoV-2 Delta-18 B.1.351v3/wtRBD
FEATURES             Location/Qualifiers
     source          1..10453
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    3844..4432
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3897..3917)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4315..4326)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    4485..4606
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4496..4515)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4550..4569
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     terminator      4721..4767
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      4797..4933
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    4978..5049
                     /label=3' beta-globin insulator
                     /note="insulator downstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     promoter        5094..5290
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      5141..5276
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5304..6098
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5358..5377)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5968..5987
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6109..6333
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(6529..7117)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6609..6628)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             join(7863..7931,7932..8723)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8081..8100)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     terminator      8736..8872
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    9024..9095
                     /label=5' beta-globin insulator
                     /note="insulator upstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     terminator      complement(9100..9146)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     polyA_signal    9164..9212
                     /note="synthetic polyadenylation signal"
     enhancer        9293..9596
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9598..9800
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     9751..9771
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     intron          9934..10409
                     /label=beta-globin intron
                     /note="internally truncated intron from human beta-globin"
ORIGIN
        1 caaaggcggc cgccatgttt gtgttcctcg ttctcctgcc cctcgtttct tcccaatgcg
       61 tgaacctcac aactagaacg cagctcccac ccgcttacac gaactctttt actcgtggag
      121 tatactatcc cgataaagtc tttaggtcca gtgtccttca ttctactcaa gaccttttcc
      181 tgccattctt ctcaaatgtc acctggttcc atgctatcca cgtttctggc accaacggaa
      241 ctaaacgatt tgctaatccc gtcttgccat ttaacgatgg cgtatacttt gcctcaacgg
      301 agaagtccaa cattatccgg ggatggatat ttggcaccac cctggactcc aagacacagt
      361 ctctcctcat tgtgaacaac gccaccaatg tcgtcatcaa agtctgtgaa tttcaatttt
      421 gtaacgatcc ctttctcggg gtttattatc ataagaataa caagtcttgg atggagtcag
      481 agttccgcgt ttacagttcc gctaacaact gtacttttga gtacgtttcc caaccatttc
      541 tcatggatct tgaagggaag caaggcaact tcaagaatct ccgcgaattt gtgtttaaga
      601 atatagacgg ctacttcaaa atttatagca agcatacccc gataaatctg gttcgggatc
      661 tccctcaagg cttctccgcg ttggaacccc tcgtggactt gccgattggc atcaacatca
      721 cccggttcca aacccttcat ataagctacc tcacgcctgg cgacagtagt agtggttgga
      781 cagcgggcgc agcagcctac tatgtgggat atttgcaacc acgcaccttt cttctcaaat
      841 ataacgaaaa cggaacaatt accgatgccg tagactgtgc cctcgatccc ctgtccgaaa
      901 ctaaatgcac actcaagtcc ttcacagttg agaaaggcat ataccaaacc agtaacttcc
      961 gcgtccaacc caccgaatcc attgttcgat tccctaacat caccaatttg tgtcctttcg
     1021 gtgaagtatt taatgccact cgatttgcat cagtttatgc ctggaatcgc aagcgaatat
     1081 caaattgtgt tgcagattat agtgtcctgt ataatagcgc atcattcagc acttttaagt
     1141 gttatggggt cagccctacg aaattgaatg acttgtgttt taccaacgtt tacgcagatt
     1201 ctttcgtcat acgcggcgac gaagtccgac agattgcacc ggggcaaacc ggaaagatag
     1261 cagactataa ttataagttg ccggatgact tcaccggctg cgttatagca tggaactcca
     1321 ataatctcga ttcaaaagtc ggcggcaact ataattatct ttaccgcctg tttcgcaagt
     1381 caaatctgaa gccctttgaa cgtgacatct ccaccgaaat ttatcaagct ggcagcaccc
     1441 catgtaatgg agtggaaggg tttaactgtt attttcccct tcaatcatac ggatttcaac
     1501 caacaaatgg ggtgggctat caaccatatc gggtggtagt cttgagcttt gagctgctcc
     1561 acgcaccggc tacagtttgc ggaccgaaga aaagcactaa cctggtgaag aataaatgtg
     1621 tgaacttcaa ctttaatggg ctcaccggaa caggggtgct gacagaatca aacaagaaat
     1681 ttctcccttt tcaacaattt ggtagagaca ttgccgatac cacagatgcc gtccgcgatc
     1741 cgcaaacact ggaaatcctc gatataacac cctgtagctt cggcggggtc agcgtgataa
     1801 ctcccggaac gaacacatcc aaccaagtgg ctgtcctgta tcaaggtgtc aactgtaccg
     1861 aagtccctgt ggcgatacac gccgaccaac tgacacccac ctggagagtc tactcaacag
     1921 gttccaatgt ctttcaaact agagcgggtt gtctcattgg cgctgagcat gtcaataatt
     1981 catacgaatg tgatattcct attggggcag gaatctgtgc gtcttatcaa actcaaacga
     2041 actcaccacg gcgcgctcgc tcagtagcca gccagtccat aatcgcctat accatgagtc
     2101 tcggggttga gaactccgtg gcttattcaa acaattccat cgcaatccca actaacttta
     2161 ccattagcgt taccacggaa atcttgcccg tcagcatgac taagacgagc gtagactgta
     2221 cgatgtatat ctgtggggat tccaccgagt gtagcaacct cttgcttcaa tacgggtctt
     2281 tctgtactca actcaaccga gcattgaccg gaattgcggt cgaacaagac aagaataccc
     2341 aagaggtgtt cgcacaagta aagcaaatat acaaaacgcc gccaatcaaa gattttggcg
     2401 ggtttaattt tagccaaata ctgccagacc catctaagcc ttcaaaacgc agctttatcg
     2461 aggatctgct gttcaacaaa gttacgttgg ctgacgcggg tttcattaag cagtatgggg
     2521 attgtttggg agacattgcg gcccgcgacc ttatctgtgc tcaaaaattc aatgggttga
     2581 cagttctgcc ccctctgctc actgatgaga tgattgcaca gtatacatca gcattgttgg
     2641 caggaaccat tacatccggc tggacttttg gcgcgggtgc ggcccttcaa atacctttcg
     2701 ctatgcaaat ggcttacaga ttcaatggga ttggtgtcac gcaaaatgta ttgtatgaaa
     2761 atcagaagct gatcgctaat caatttaaca gcgcgattgg caagatccaa gattcactgt
     2821 caagcacagc gtccgccctc ggcaaactcc aagatgttgt gaatcaaaac gcccaggccc
     2881 tgaataccct cgtgaagcaa ctgagttcta acttcggcgc aatctccagc gtcctcaacg
     2941 acatattgtc cagacttgat aaggtggaag ccgaggtgca aattgatcgg ctcatcacag
     3001 gcaggctcca gtcactccaa acttatgtga cacaacagtt gatccgggct gctgagatac
     3061 gagcaagcgc caacctggcc gccactaaaa tgtcagagtg cgtgctcggg cagtccaaac
     3121 gggttgactt ttgtggcaag ggctatcact tgatgtcctt cccccaatcc gccccacatg
     3181 gagtggtctt tctccacgtc acttatgtcc ctgctcaaga gaagaacttt acgacagctc
     3241 cagcaatctg tcacgatggc aaagcgcact ttcccaggga aggcgttttc gtcagtaatg
     3301 gcacgcattg gtttgtgacc caaaggaatt tctatgaacc ccaaatcata accactgaca
     3361 atacatttgt ctcaggcaac tgtgatgtgg tcattgggat cgtgaacaac acggtatatg
     3421 atcccctgca acccgagttg gatagcttca aagaggaatt ggataaatac tttaagaatc
     3481 atacttcacc cgatgttgat ctcggggaca tatcaggaat aaatgcaagt gtcgtcaaca
     3541 ttcagaaaga gattgatcgc cttaatgagg tcgccaagaa cttgaatgaa tctttgatcg
     3601 acttgcaaga actgggcaaa tacgaacaat atattaaatg gccctggtac atttggttgg
     3661 gctttatcgc cggacttata gctatcgtta tggtcacaat tatgttgtgc tgtatgacta
     3721 gctgttgttc ctgcttgaaa ggatgttgca gttgcggatc ttgttgtaaa taaaggatcc
     3781 tctagaagtt gtctcctcct gcactgactg actgatacaa tcgatttctg gatccgcagg
     3841 cctaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
     3901 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
     3961 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag
     4021 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc
     4081 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc
     4141 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg
     4201 ctgttgggca ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt tccatggctg
     4261 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc
     4321 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt
     4381 cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc gcctgtctag
     4441 cttgactgac tgagatacag cgtaccttca gctcacagac atgataagat acattgatga
     4501 gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga
     4561 tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg
     4621 cattcatttt atgtttcagg ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa
     4681 cctctacaaa tgtggtattg gcccatctct atcggtatcg tagcataacc ccttggggcc
     4741 tctaaacggg tcttgagggg ttttttgtgc ccctcgggcc ggattgctat ctaccggcat
     4801 tggcgcagaa aaaaatgcct gatgcgacgc tgcgcgtctt atactcccac atatgccaga
     4861 ttcagcaacg gatacggctt ccccaacttg cccacttcca tacgtgtcct ccttaccaga
     4921 aatttatcct taaggtcgtc agctatcctg caggcgatct ctcgatttcg atcaagacat
     4981 tcctttaatg gtcttttctg gacaccacta ggggtcagaa gtagttcatc aaactttctt
     5041 ccctccctaa tctcattggt taccttgggc tatcgaaact taattaagcg atctgcatct
     5101 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc
     5161 cagttccgcc cattctccgc cccatcgctg actaattttt tttatttatg cagaggccga
     5221 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     5281 cttttgcaaa ggaggtagcc aacatgattg aacaagatgg attgcacgca ggttctcccg
     5341 ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg
     5401 atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc
     5461 tgtccggtgc cctgaatgaa ctccaggacg aggcagcgcg gctatcgtgg ctggccacga
     5521 cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc
     5581 tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag
     5641 tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat
     5701 tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg
     5761 tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca
     5821 ggctcaaggc gcggatgccc gacggcgagg atctcgtcgt gacccacggc gatgcctgct
     5881 tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg
     5941 gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg
     6001 gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc
     6061 gcatcgcctt ctatcgcctt cttgacgagt tcttctagta tgtaagccct gtgccttcta
     6121 gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca
     6181 ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc
     6241 attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata
     6301 gcaggcatgc tggggatgcg gtgggctcta tggttaatta accagtcaag tcagctactt
     6361 ggcgagatcg acttgtctgg gtttcgacta cgctcagaat tgcgtcagtc aagttcgatc
     6421 tggtccttgc tattgcaccc gttctccgat tacgagtttc atttaaatca tgtgagcaaa
     6481 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     6541 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     6601 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     6661 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     6721 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     6781 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     6841 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     6901 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     6961 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     7021 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     7081 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     7141 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     7201 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     7261 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     7321 agcgatctgt ctatttcgtt catccatagt tgcatttaaa tttccgaact ctccaaggcc
     7381 ctcgtcggaa aatcttcaaa cctttcgtcc gatccatctt gcaggctacc tctcgaacga
     7441 actatcgcaa gtctcttggc cggccttgcg ccttggctat tgcttggcag cgcctatcgc
     7501 caggtattac tccaatcccg aatatccgag atcgggatca cccgagagaa gttcaaccta
     7561 catcctcaat cccgatctat ccgagatccg aggaatatcg aaatcggggc gcgcctggtg
     7621 taccgagaac gatcctctca gtgcgagtct cgacgatcca tatcgttgct tggcagtcag
     7681 ccagtcggaa tccagcttgg gacccaggaa gtccaatcgt cagatattgt actcaagcct
     7741 ggtcacggca gcgtaccgat ctgtttaaac ctagatattg atagtctgat cggtcaacgt
     7801 ataatcgagt cctagctttt gcaaacatct atcaagagac aggatcagca ggaggctttc
     7861 gcatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     7921 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     7981 cgcgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     8041 ccgaagaacg ctttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     8101 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     8161 tggttgagta ttcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     8221 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     8281 ttggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     8341 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     8401 tgcctgtagc aatggcaaca accttgcgta aactattaac tggcgaacta cttactctag
     8461 cttcccggca acagttgata gactggatgg aggcggataa agttgcagga ccacttctgc
     8521 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     8581 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     8641 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     8701 cctcactgat taagcattgg taaccgattc taggtgcatt ggcgcagaaa aaaatgcctg
     8761 atgcgacgct gcgcgtctta tactcccaca tatgccagat tcagcaacgg atacggcttc
     8821 cccaacttgc ccacttccat acgtgtcctc cttaccagaa atttatcctt aagatcccga
     8881 atcgtttaaa ctcgactctg gctctatcga atctccgtcg tttcgagctt acgcgaacag
     8941 ccgtggcgct catttgctcg tcgggcatcg aatctcgtca gctatcgtca gcttaccttt
     9001 ttggcagcga tcgcggctcc cgacatcttg gaccattagc tccacaggta tcttcttccc
     9061 tctagtggtc ataacagcag cttcagctac ctctcaattc aaaaaacccc tcaagacccg
     9121 tttagaggcc ccaaggggtt atgctatcaa tcgttgcgtt acacacacaa aaaaccaaca
     9181 cacatccatc ttcgatggat agcgatttta ttatctaact gctgatcgag tgtagccaga
     9241 tctagtaatc aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat
     9301 aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa
     9361 taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg
     9421 agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc
     9481 cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct
     9541 tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatgctga
     9601 tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa
     9661 gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc
     9721 caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg
     9781 aggtctatat aagcagagct ggtttagtga accgtcagat cgcctggaga cgccatccac
     9841 gctgttttga cctccataga agacaccggg accgatccag cctcccctcg aagcttacat
     9901 gtggtaccga gctcggatcc tgagaacttc agggtgagtc tatgggaccc ttgatgtttt
     9961 ctttcccctt cttttctatg gttaagttca tgtcatagga aggggagaag taacagggta
    10021 cacatattga ccaaatcagg gtaattttgc atttgtaatt ttaaaaaatg ctttcttctt
    10081 ttaatatact tttttgttta tcttatttct aatactttcc ctaatctctt tctttcaggg
    10141 caataatgat acaatgtatc atgcctcttt gcaccattct aaagaataac agtgataatt
    10201 tctgggttaa ggcaatagca atatttctgc atataaatat ttctgcatat aaattgtaac
    10261 tgatgtaaga ggtttcatat tgctaatagc agctacaatc cagctaccat tctgctttta
    10321 ttttatggtt gggataaggc tggattattc tgagtccaag ctaggccctt ttgctaatca
    10381 tgttcatacc tcttatcttc ctcccacagc tcctgggcaa cgtgctggtc tgtgtgctgg
    10441 cccatcactt tgg
//
