LOCUS       sequence-335491-       10462 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes SARS-CoV-2 K417N+E484K+N501Y Spike Protein lacking 18 
            C-terminal amino acids for pseudovirus production.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10462)
  AUTHORS   Garcia-Beltran WF, Lam EC, St Denis K, Nitido AD, Garcia ZH, Hauser 
            BM, Feldman J, Pavlovic MN, Gregory DJ, Poznansky MC, Sigal A, 
            Schmidt AG, Iafrate AJ, Naranbhai V, Balazs AB
  TITLE     Multiple SARS-CoV-2 variants escape neutralization by 
            vaccine-induced humoral immunity.
  JOURNAL   Cell. 2021 Mar 12. pii: S0092-8674(21)00298-1. doi: 
            10.1016/j.cell.2021.03.013.
  PUBMED    33743213
REFERENCE   2  (bases 1 to 10462)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2021 
            Mar 12. pii: S0092-8674(21)00298-1. doi: 
            10.1016/j.cell.2021.03.013."
COMMENT     Sequence Label: pTwist-SARS-CoV-2 Delta-18 K417N+E484K+N501Y
FEATURES             Location/Qualifiers
     source          1..10462
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    3851..4439
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3904..3924)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4322..4333)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    4492..4613
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4503..4522)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4557..4576
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     terminator      4728..4774
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      4804..4940
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    4985..5056
                     /label=3' beta-globin insulator
                     /note="insulator downstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     promoter        5101..5297
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      5148..5283
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5311..6105
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5365..5384)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5975..5994
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6116..6340
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(6536..7124)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6616..6635)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             join(7870..7938,7939..8730)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8088..8107)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     terminator      8743..8879
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    9031..9102
                     /label=5' beta-globin insulator
                     /note="insulator upstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     terminator      complement(9107..9153)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     polyA_signal    9171..9219
                     /note="synthetic polyadenylation signal"
     enhancer        9300..9603
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9605..9807
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     9758..9778
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     intron          9941..10416
                     /label=beta-globin intron
                     /note="internally truncated intron from human beta-globin"
ORIGIN
        1 aaggcggccg ccatgtttgt gttcctcgtt ctcctgcccc tcgtttcttc ccaatgcgtg
       61 aacctcacaa ctagaacgca gctcccaccc gcttacacga actcttttac tcgtggagta
      121 tactatcccg ataaagtctt taggtccagt gtccttcatt ctactcaaga ccttttcctg
      181 ccattcttct caaatgtcac ctggttccat gctatccacg tttctggcac caacggaact
      241 aaacgatttg ataatcccgt cttgccattt aacgatggcg tatactttgc ctcaacggag
      301 aagtccaaca ttatccgggg atggatattt ggcaccaccc tggactccaa gacacagtct
      361 ctcctcattg tgaacaacgc caccaatgtc gtcatcaaag tctgtgaatt tcaattttgt
      421 aacgatccct ttctcggggt ttattatcat aagaataaca agtcttggat ggagtcagag
      481 ttccgcgttt acagttccgc taacaactgt acttttgagt acgtttccca accatttctc
      541 atggatcttg aagggaagca aggcaacttc aagaatctcc gcgaatttgt gtttaagaat
      601 atagacggct acttcaaaat ttatagcaag cataccccga taaatctggt tcgggatctc
      661 cctcaaggct tctccgcgtt ggaacccctc gtggacttgc cgattggcat caacatcacc
      721 cggttccaaa cccttcttgc ccttcatcga agctacctca cgcctggcga cagtagtagt
      781 ggttggacag cgggcgcagc agcctactat gtgggatatt tgcaaccacg cacctttctt
      841 ctcaaatata acgaaaacgg aacaattacc gatgccgtag actgtgccct cgatcccctg
      901 tccgaaacta aatgcacact caagtccttc acagttgaga aaggcatata ccaaaccagt
      961 aacttccgcg tccaacccac cgaatccatt gttcgattcc ctaacatcac caatttgtgt
     1021 cctttcggtg aagtatttaa tgccactcga tttgcatcag tttatgcctg gaatcgcaag
     1081 cgaatatcaa attgtgttgc agattatagt gtcctgtata atagcgcatc attcagcact
     1141 tttaagtgtt atggggtcag ccctacgaaa ttgaatgact tgtgttttac caacgtttac
     1201 gcagattctt tcgtcatacg cggcgacgaa gtccgacaga ttgcaccggg gcaaaccgga
     1261 aatatagcag actataatta taagttgccg gatgacttca ccggctgcgt tatagcatgg
     1321 aactccaata atctcgattc aaaagtcggc ggcaactata attatcttta ccgcctgttt
     1381 cgcaagtcaa atctgaagcc ctttgaacgt gacatctcca ccgaaattta tcaagctggc
     1441 agcaccccat gtaatggagt gaaagggttt aactgttatt ttccccttca atcatacgga
     1501 tttcaaccaa catatggggt gggctatcaa ccatatcggg tggtagtctt gagctttgag
     1561 ctgctccacg caccggctac agtttgcgga ccgaagaaaa gcactaacct ggtgaagaat
     1621 aaatgtgtga acttcaactt taatgggctc accggaacag gggtgctgac agaatcaaac
     1681 aagaaatttc tcccttttca acaatttggt agagacattg ccgataccac agatgccgtc
     1741 cgcgatccgc aaacactgga aatcctcgat ataacaccct gtagcttcgg cggggtcagc
     1801 gtgataactc ccggaacgaa cacatccaac caagtggctg tcctgtatca agatgtcaac
     1861 tgtaccgaag tccctgtggc gatacacgcc gaccaactga cacccacctg gagagtctac
     1921 tcaacaggtt ccaatgtctt tcaaactaga gcgggttgtc tcattggcgc tgagcatgtc
     1981 aataattcat acgaatgtga tattcctatt ggggcaggaa tctgtgcgtc ttatcaaact
     2041 caaacgaact caccacggcg cgctcgctca gtagccagcc agtccataat cgcctatacc
     2101 atgagtctcg gggctgagaa ctccgtggct tattcaaaca attccatcgc aatcccaact
     2161 aactttacca ttagcgttac cacggaaatc ttgcccgtca gcatgactaa gacgagcgta
     2221 gactgtacga tgtatatctg tggggattcc accgagtgta gcaacctctt gcttcaatac
     2281 gggtctttct gtactcaact caaccgagca ttgaccggaa ttgcggtcga acaagacaag
     2341 aatacccaag aggtgttcgc acaagtaaag caaatataca aaacgccgcc aatcaaagat
     2401 tttggcgggt ttaattttag ccaaatactg ccagacccat ctaagccttc aaaacgcagc
     2461 tttatcgagg atctgctgtt caacaaagtt acgttggctg acgcgggttt cattaagcag
     2521 tatggggatt gtttgggaga cattgcggcc cgcgacctta tctgtgctca aaaattcaat
     2581 gggttgacag ttctgccccc tctgctcact gatgagatga ttgcacagta tacatcagca
     2641 ttgttggcag gaaccattac atccggctgg acttttggcg cgggtgcggc ccttcaaata
     2701 cctttcgcta tgcaaatggc ttacagattc aatgggattg gtgtcacgca aaatgtattg
     2761 tatgaaaatc agaagctgat cgctaatcaa tttaacagcg cgattggcaa gatccaagat
     2821 tcactgtcaa gcacagcgtc cgccctcggc aaactccaag atgttgtgaa tcaaaacgcc
     2881 caggccctga ataccctcgt gaagcaactg agttctaact tcggcgcaat ctccagcgtc
     2941 ctcaacgaca tattgtccag acttgataag gtggaagccg aggtgcaaat tgatcggctc
     3001 atcacaggca ggctccagtc actccaaact tatgtgacac aacagttgat ccgggctgct
     3061 gagatacgag caagcgccaa cctggccgcc actaaaatgt cagagtgcgt gctcgggcag
     3121 tccaaacggg ttgacttttg tggcaagggc tatcacttga tgtccttccc ccaatccgcc
     3181 ccacatggag tggtctttct ccacgtcact tatgtccctg ctcaagagaa gaactttacg
     3241 acagctccag caatctgtca cgatggcaaa gcgcactttc ccagggaagg cgttttcgtc
     3301 agtaatggca cgcattggtt tgtgacccaa aggaatttct atgaacccca aatcataacc
     3361 actgacaata catttgtctc aggcaactgt gatgtggtca ttgggatcgt gaacaacacg
     3421 gtatatgatc ccctgcaacc cgagttggat agcttcaaag aggaattgga taaatacttt
     3481 aagaatcata cttcacccga tgttgatctc ggggacatat caggaataaa tgcaagtgtc
     3541 gtcaacattc agaaagagat tgatcgcctt aatgaggtcg ccaagaactt gaatgaatct
     3601 ttgatcgact tgcaagaact gggcaaatac gaacaatata ttaaatggcc ctggtacatt
     3661 tggttgggct ttatcgccgg acttatagct atcgttatgg tcacaattat gttgtgctgt
     3721 atgactagct gttgttcctg cttgaaagga tgttgcagtt gcggatcttg ttgtaaataa
     3781 aggatcctct agaagttgtc tcctcctgca ctgactgact gatacaatcg atttctggat
     3841 ccgcaggcct aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa
     3901 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat
     3961 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta
     4021 tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc
     4081 aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt
     4141 ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg
     4201 ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaagctga cgtcctttcc
     4261 atggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc
     4321 ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct
     4381 tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcc
     4441 tgtctagctt gactgactga gatacagcgt accttcagct cacagacatg ataagataca
     4501 ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa
     4561 tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca
     4621 acaattgcat tcattttatg tttcaggttc agggggaggt gtgggaggtt ttttaaagca
     4681 agtaaaacct ctacaaatgt ggtattggcc catctctatc ggtatcgtag cataacccct
     4741 tggggcctct aaacgggtct tgaggggttt tttgtgcccc tcgggccgga ttgctatcta
     4801 ccggcattgg cgcagaaaaa aatgcctgat gcgacgctgc gcgtcttata ctcccacata
     4861 tgccagattc agcaacggat acggcttccc caacttgccc acttccatac gtgtcctcct
     4921 taccagaaat ttatccttaa ggtcgtcagc tatcctgcag gcgatctctc gatttcgatc
     4981 aagacattcc tttaatggtc ttttctggac accactaggg gtcagaagta gttcatcaaa
     5041 ctttcttccc tccctaatct cattggttac cttgggctat cgaaacttaa ttaagcgatc
     5101 tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa
     5161 ctccgcccag ttccgcccat tctccgcccc atcgctgact aatttttttt atttatgcag
     5221 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     5281 gcctaggctt ttgcaaagga ggtagccaac atgattgaac aagatggatt gcacgcaggt
     5341 tctcccgccg cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc
     5401 tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag
     5461 accgacctgt ccggtgccct gaatgaactc caggacgagg cagcgcggct atcgtggctg
     5521 gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac
     5581 tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc
     5641 gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc
     5701 tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc
     5761 ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg
     5821 ttcgccaggc tcaaggcgcg gatgcccgac ggcgaggatc tcgtcgtgac ccacggcgat
     5881 gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc
     5941 cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa
     6001 gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat
     6061 tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctagtatgt aagccctgtg
     6121 ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa
     6181 ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt
     6241 aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa
     6301 gacaatagca ggcatgctgg ggatgcggtg ggctctatgg ttaattaacc agtcaagtca
     6361 gctacttggc gagatcgact tgtctgggtt tcgactacgc tcagaattgc gtcagtcaag
     6421 ttcgatctgg tccttgctat tgcacccgtt ctccgattac gagtttcatt taaatcatgt
     6481 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     6541 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     6601 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     6661 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     6721 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     6781 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     6841 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     6901 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     6961 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     7021 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     7081 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     7141 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     7201 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     7261 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     7321 ctatctcagc gatctgtcta tttcgttcat ccatagttgc atttaaattt ccgaactctc
     7381 caaggccctc gtcggaaaat cttcaaacct ttcgtccgat ccatcttgca ggctacctct
     7441 cgaacgaact atcgcaagtc tcttggccgg ccttgcgcct tggctattgc ttggcagcgc
     7501 ctatcgccag gtattactcc aatcccgaat atccgagatc gggatcaccc gagagaagtt
     7561 caacctacat cctcaatccc gatctatccg agatccgagg aatatcgaaa tcggggcgcg
     7621 cctggtgtac cgagaacgat cctctcagtg cgagtctcga cgatccatat cgttgcttgg
     7681 cagtcagcca gtcggaatcc agcttgggac ccaggaagtc caatcgtcag atattgtact
     7741 caagcctggt cacggcagcg taccgatctg tttaaaccta gatattgata gtctgatcgg
     7801 tcaacgtata atcgagtcct agcttttgca aacatctatc aagagacagg atcagcagga
     7861 ggctttcgca tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
     7921 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
     7981 ttgggtgcgc gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
     8041 tttcgccccg aagaacgctt tccaatgatg agcactttta aagttctgct atgtggcgcg
     8101 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
     8161 aatgacttgg ttgagtattc accagtcaca gaaaagcatc ttacggatgg catgacagta
     8221 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
     8281 acaacgattg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
     8341 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     8401 accacgatgc ctgtagcaat ggcaacaacc ttgcgtaaac tattaactgg cgaactactt
     8461 actctagctt cccggcaaca gttgatagac tggatggagg cggataaagt tgcaggacca
     8521 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     8581 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
     8641 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     8701 ataggtgcct cactgattaa gcattggtaa ccgattctag gtgcattggc gcagaaaaaa
     8761 atgcctgatg cgacgctgcg cgtcttatac tcccacatat gccagattca gcaacggata
     8821 cggcttcccc aacttgccca cttccatacg tgtcctcctt accagaaatt tatccttaag
     8881 atcccgaatc gtttaaactc gactctggct ctatcgaatc tccgtcgttt cgagcttacg
     8941 cgaacagccg tggcgctcat ttgctcgtcg ggcatcgaat ctcgtcagct atcgtcagct
     9001 tacctttttg gcagcgatcg cggctcccga catcttggac cattagctcc acaggtatct
     9061 tcttccctct agtggtcata acagcagctt cagctacctc tcaattcaaa aaacccctca
     9121 agacccgttt agaggcccca aggggttatg ctatcaatcg ttgcgttaca cacacaaaaa
     9181 accaacacac atccatcttc gatggatagc gattttatta tctaactgct gatcgagtgt
     9241 agccagatct agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc
     9301 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
     9361 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
     9421 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
     9481 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
     9541 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
     9601 atgctgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga
     9661 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg
     9721 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta
     9781 cggtgggagg tctatataag cagagctggt ttagtgaacc gtcagatcgc ctggagacgc
     9841 catccacgct gttttgacct ccatagaaga caccgggacc gatccagcct cccctcgaag
     9901 cttacatgtg gtaccgagct cggatcctga gaacttcagg gtgagtctat gggacccttg
     9961 atgttttctt tccccttctt ttctatggtt aagttcatgt cataggaagg ggagaagtaa
    10021 cagggtacac atattgacca aatcagggta attttgcatt tgtaatttta aaaaatgctt
    10081 tcttctttta atatactttt ttgtttatct tatttctaat actttcccta atctctttct
    10141 ttcagggcaa taatgataca atgtatcatg cctctttgca ccattctaaa gaataacagt
    10201 gataatttct gggttaaggc aatagcaata tttctgcata taaatatttc tgcatataaa
    10261 ttgtaactga tgtaagaggt ttcatattgc taatagcagc tacaatccag ctaccattct
    10321 gcttttattt tatggttggg ataaggctgg attattctga gtccaagcta ggcccttttg
    10381 ctaatcatgt tcatacctct tatcttcctc ccacagctcc tgggcaacgt gctggtctgt
    10441 gtgctggccc atcactttgg ca
//
