LOCUS       sequence-335492-       10452 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes SARS-CoV-2 B.1.351v2/wtRBD Spike Protein lacking 18 
            C-terminal amino acids for pseudovirus production.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10452)
  AUTHORS   Garcia-Beltran WF, Lam EC, St Denis K, Nitido AD, Garcia ZH, Hauser 
            BM, Feldman J, Pavlovic MN, Gregory DJ, Poznansky MC, Sigal A, 
            Schmidt AG, Iafrate AJ, Naranbhai V, Balazs AB
  TITLE     Multiple SARS-CoV-2 variants escape neutralization by 
            vaccine-induced humoral immunity.
  JOURNAL   Cell. 2021 Mar 12. pii: S0092-8674(21)00298-1. doi: 
            10.1016/j.cell.2021.03.013.
  PUBMED    33743213
REFERENCE   2  (bases 1 to 10452)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2021 
            Mar 12. pii: S0092-8674(21)00298-1. doi: 
            10.1016/j.cell.2021.03.013."
COMMENT     Sequence Label: pTwist-SARS-CoV-2 Delta-18 B.1.351v2/wtRBD
FEATURES             Location/Qualifiers
     source          1..10452
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    3931..4519
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3984..4004)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4402..4413)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    4572..4693
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4583..4602)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4637..4656
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     terminator      4808..4854
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      4884..5020
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    5065..5136
                     /label=3' beta-globin insulator
                     /note="insulator downstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     promoter        5181..5377
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      5228..5363
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5391..6185
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5445..5464)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6055..6074
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6196..6420
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(6616..7204)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6696..6715)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             join(7950..8018,8019..8810)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8168..8187)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     terminator      8823..8959
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    9111..9182
                     /label=5' beta-globin insulator
                     /note="insulator upstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     terminator      complement(9187..9233)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     polyA_signal    9251..9299
                     /note="synthetic polyadenylation signal"
     enhancer        9380..9683
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9685..9887
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     9838..9858
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     intron          join(10021..10452,1..44)
                     /label=beta-globin intron
                     /note="internally truncated intron from human beta-globin"
ORIGIN
        1 cccttttgct aatcatgttc atacctctta tcttcctccc acagctcctg ggcaacgtgc
       61 tggtctgtgt gctggcccat cactttggca aaggcggcgc catgtttgtg ttcctcgttc
      121 tcctgcccct cgtttcttcc caatgcgtga acttcacaac tagaacgcag ctcccacccg
      181 cttacacgaa ctcttttact cgtggagtat actatcccga taaagtcttt aggtccagtg
      241 tccttcattc tactcaagac cttttcctgc cattcttctc aaatgtcacc tggttccatg
      301 ctatccacgt ttctggcacc aacggaacta aacgatttgc taatcccgtc ttgccattta
      361 acgatggcgt atactttgcc tcaacggaga agtccaacat tatccgggga tggatatttg
      421 gcaccaccct ggactccaag acacagtctc tcctcattgt gaacaacgcc accaatgtcg
      481 tcatcaaagt ctgtgaattt caattttgta acgatccctt tctcggggtt tattatcata
      541 agaataacaa gtcttggatg gagtcagagt tccgcgttta cagttccgct aacaactgta
      601 cttttgagta cgtttcccaa ccatttctca tggatcttga agggaagcaa ggcaacttca
      661 agaatctccg cgaatttgtg tttaagaata tagacggcta cttcaaaatt tatagcaagc
      721 ataccccgat aaatctggtt cggggtctcc ctcaaggctt ctccgcgttg gaacccctcg
      781 tggacttgcc gattggcatc aacatcaccc ggttccaaac ccttcatcga agctacctca
      841 cgcctggcga cagtagtagt ggttggacag cgggcgcagc agcctactat gtgggatatt
      901 tgcaaccacg cacctttctt ctcaaatata acgaaaacgg aacaattacc gatgccgtag
      961 actgtgccct cgatcccctg tccgaaacta aatgcacact caagtccttc acagttgaga
     1021 aaggcatata ccaaaccagt aacttccgcg tccaacccac cgaatccatt gttcgattcc
     1081 ctaacatcac caatttgtgt cctttcggtg aagtatttaa tgccactcga tttgcatcag
     1141 tttatgcctg gaatcgcaag cgaatatcaa attgtgttgc agattatagt gtcctgtata
     1201 atagcgcatc attcagcact tttaagtgtt atggggtcag ccctacgaaa ttgaatgact
     1261 tgtgttttac caacgtttac gcagattctt tcgtcatacg cggcgacgaa gtccgacaga
     1321 ttgcaccggg gcaaaccgga aagatagcag actataatta taagttgccg gatgacttca
     1381 ccggctgcgt tatagcatgg aactccaata atctcgattc aaaagtcggc ggcaactata
     1441 attatcttta ccgcctgttt cgcaagtcaa atctgaagcc ctttgaacgt gacatctcca
     1501 ccgaaattta tcaagctggc agcaccccat gtaatggagt ggaagggttt aactgttatt
     1561 ttccccttca atcatacgga tttcaaccaa caaatggggt gggctatcaa ccatatcggg
     1621 tggtagtctt gagctttgag ctgctccacg caccggctac agtttgcgga ccgaagaaaa
     1681 gcactaacct ggtgaagaat aaatgtgtga acttcaactt taatgggctc accggaacag
     1741 gggtgctgac agaatcaaac aagaaatttc tcccttttca acaatttggt agagacattg
     1801 ccgataccac agatgccgtc cgcgatccgc aaacactgga aatcctcgat ataacaccct
     1861 gtagcttcgg cggggtcagc gtgataactc ccggaacgaa cacatccaac caagtggctg
     1921 tcctgtatca aggtgtcaac tgtaccgaag tccctgtggc gatacacgcc gaccaactga
     1981 cacccacctg gagagtctac tcaacaggtt ccaatgtctt tcaaactaga gcgggttgtc
     2041 tcattggcgc tgagcatgtc aataattcat acgaatgtga tattcctatt ggggcaggaa
     2101 tctgtgcgtc ttatcaaact caaacgaact caccacggcg cgctcgctca gtagccagcc
     2161 agtccataat cgcctatacc atgagtctcg gggttgagaa ctccgtggct tattcaaaca
     2221 attccatcgc aatcccaact aactttacca ttagcgttac cacggaaatc ttgcccgtca
     2281 gcatgactaa gacgagcgta gactgtacga tgtatatctg tggggattcc accgagtgta
     2341 gcaacctctt gcttcaatac gggtctttct gtactcaact caaccgagca ttgaccggaa
     2401 ttgcggtcga acaagacaag aatacccaag aggtgttcgc acaagtaaag caaatataca
     2461 aaacgccgcc aatcaaagat tttggcgggt ttaattttag ccaaatactg ccagacccat
     2521 ctaagccttc aaaacgcagc tttatcgagg atctgctgtt caacaaagtt acgttggctg
     2581 acgcgggttt cattaagcag tatggggatt gtttgggaga cattgcggcc cgcgacctta
     2641 tctgtgctca aaaattcaat gggttgacag ttctgccccc tctgctcact gatgagatga
     2701 ttgcacagta tacatcagca ttgttggcag gaaccattac atccggctgg acttttggcg
     2761 cgggtgcggc ccttcaaata cctttcgcta tgcaaatggc ttacagattc aatgggattg
     2821 gtgtcacgca aaatgtattg tatgaaaatc agaagctgat cgctaatcaa tttaacagcg
     2881 cgattggcaa gatccaagat tcactgtcaa gcacagcgtc cgccctcggc aaactccaag
     2941 atgttgtgaa tcaaaacgcc caggccctga ataccctcgt gaagcaactg agttctaact
     3001 tcggcgcaat ctccagcgtc ctcaacgaca tattgtccag acttgataag gtggaagccg
     3061 aggtgcaaat tgatcggctc atcacaggca ggctccagtc actccaaact tatgtgacac
     3121 aacagttgat ccgggctgct gagatacgag caagcgccaa cctggccgcc actaaaatgt
     3181 cagagtgcgt gctcgggcag tccaaacggg ttgacttttg tggcaagggc tatcacttga
     3241 tgtccttccc ccaatccgcc ccacatggag tggtctttct ccacgtcact tatgtccctg
     3301 ctcaagagaa gaactttacg acagctccag caatctgtca cgatggcaaa gcgcactttc
     3361 ccagggaagg cgttttcgtc agtaatggca cgcattggtt tgtgacccaa aggaatttct
     3421 atgaacccca aatcataacc actgacaata catttgtctc aggcaactgt gatgtggtca
     3481 ttgggatcgt gaacaacacg gtatatgatc ccctgcaacc cgagttggat agcttcaaag
     3541 aggaattgga taaatacttt aagaatcata cttcacccga tgttgatctc ggggacatat
     3601 caggaataaa tgcaagtgtc gtcaacattc agaaagagat tgatcgcctt aatgaggtcg
     3661 ccaagaactt gaatgaatct ttgatcgact tgcaagaact gggcaaatac gaacaatata
     3721 ttaaatggcc ctggtacatt tggttgggct ttatcgccgg acttatagct atcgttatgg
     3781 tcacaattat gttgtgctgt atgactagct gttgttcctg cttgaaagga tgttgcagtt
     3841 gcggatcttg ttgtaaataa aggatcctct agaagttgtc tcctcctgca ctgactgact
     3901 gatacaatcg atttctggat ccgcaggcct aatcaacctc tggattacaa aatttgtgaa
     3961 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
     4021 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
     4081 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
     4141 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
     4201 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
     4261 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
     4321 gggaagctga cgtcctttcc atggctgctc gcctgtgttg ccacctggat tctgcgcggg
     4381 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
     4441 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc
     4501 ctttgggccg cctccccgcc tgtctagctt gactgactga gatacagcgt accttcagct
     4561 cacagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa
     4621 aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg
     4681 caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc agggggaggt
     4741 gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtattggcc catctctatc
     4801 ggtatcgtag cataacccct tggggcctct aaacgggtct tgaggggttt tttgtgcccc
     4861 tcgggccgga ttgctatcta ccggcattgg cgcagaaaaa aatgcctgat gcgacgctgc
     4921 gcgtcttata ctcccacata tgccagattc agcaacggat acggcttccc caacttgccc
     4981 acttccatac gtgtcctcct taccagaaat ttatccttaa ggtcgtcagc tatcctgcag
     5041 gcgatctctc gatttcgatc aagacattcc tttaatggtc ttttctggac accactaggg
     5101 gtcagaagta gttcatcaaa ctttcttccc tccctaatct cattggttac cttgggctat
     5161 cgaaacttaa ttaagcgatc tgcatctcaa ttagtcagca accatagtcc cgcccctaac
     5221 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atcgctgact
     5281 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
     5341 gtgaggaggc ttttttggag gcctaggctt ttgcaaagga ggtagccaac atgattgaac
     5401 aagatggatt gcacgcaggt tctcccgccg cttgggtgga gaggctattc ggctatgact
     5461 gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc
     5521 gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactc caggacgagg
     5581 cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg
     5641 tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt
     5701 catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc
     5761 atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag
     5821 cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg
     5881 ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg gatgcccgac ggcgaggatc
     5941 tcgtcgtgac ccacggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt
     6001 ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg
     6061 ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt
     6121 acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct
     6181 tctagtatgt aagccctgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg
     6241 tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa
     6301 ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca
     6361 gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg
     6421 ttaattaacc agtcaagtca gctacttggc gagatcgact tgtctgggtt tcgactacgc
     6481 tcagaattgc gtcagtcaag ttcgatctgg tccttgctat tgcacccgtt ctccgattac
     6541 gagtttcatt taaatcatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     6601 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     6661 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     6721 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     6781 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     6841 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     6901 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     6961 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     7021 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     7081 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     7141 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     7201 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     7261 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     7321 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     7381 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     7441 atttaaattt ccgaactctc caaggccctc gtcggaaaat cttcaaacct ttcgtccgat
     7501 ccatcttgca ggctacctct cgaacgaact atcgcaagtc tcttggccgg ccttgcgcct
     7561 tggctattgc ttggcagcgc ctatcgccag gtattactcc aatcccgaat atccgagatc
     7621 gggatcaccc gagagaagtt caacctacat cctcaatccc gatctatccg agatccgagg
     7681 aatatcgaaa tcggggcgcg cctggtgtac cgagaacgat cctctcagtg cgagtctcga
     7741 cgatccatat cgttgcttgg cagtcagcca gtcggaatcc agcttgggac ccaggaagtc
     7801 caatcgtcag atattgtact caagcctggt cacggcagcg taccgatctg tttaaaccta
     7861 gatattgata gtctgatcgg tcaacgtata atcgagtcct agcttttgca aacatctatc
     7921 aagagacagg atcagcagga ggctttcgca tgagtattca acatttccgt gtcgccctta
     7981 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     8041 taaaagatgc tgaagatcag ttgggtgcgc gagtgggtta catcgaactg gatctcaaca
     8101 gcggtaagat ccttgagagt tttcgccccg aagaacgctt tccaatgatg agcactttta
     8161 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     8221 gccgcataca ctattctcag aatgacttgg ttgagtattc accagtcaca gaaaagcatc
     8281 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     8341 ctgcggccaa cttacttctg acaacgattg gaggaccgaa ggagctaacc gcttttttgc
     8401 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     8461 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacc ttgcgtaaac
     8521 tattaactgg cgaactactt actctagctt cccggcaaca gttgatagac tggatggagg
     8581 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     8641 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     8701 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     8761 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ccgattctag
     8821 gtgcattggc gcagaaaaaa atgcctgatg cgacgctgcg cgtcttatac tcccacatat
     8881 gccagattca gcaacggata cggcttcccc aacttgccca cttccatacg tgtcctcctt
     8941 accagaaatt tatccttaag atcccgaatc gtttaaactc gactctggct ctatcgaatc
     9001 tccgtcgttt cgagcttacg cgaacagccg tggcgctcat ttgctcgtcg ggcatcgaat
     9061 ctcgtcagct atcgtcagct tacctttttg gcagcgatcg cggctcccga catcttggac
     9121 cattagctcc acaggtatct tcttccctct agtggtcata acagcagctt cagctacctc
     9181 tcaattcaaa aaacccctca agacccgttt agaggcccca aggggttatg ctatcaatcg
     9241 ttgcgttaca cacacaaaaa accaacacac atccatcttc gatggatagc gattttatta
     9301 tctaactgct gatcgagtgt agccagatct agtaatcaat tacggggtca ttagttcata
     9361 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
     9421 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
     9481 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
     9541 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
     9601 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
     9661 tattagtcat cgctattacc atgctgatgc ggttttggca gtacatcaat gggcgtggat
     9721 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt
     9781 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc
     9841 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctggt ttagtgaacc
     9901 gtcagatcgc ctggagacgc catccacgct gttttgacct ccatagaaga caccgggacc
     9961 gatccagcct cccctcgaag cttacatgtg gtaccgagct cggatcctga gaacttcagg
    10021 gtgagtctat gggacccttg atgttttctt tccccttctt ttctatggtt aagttcatgt
    10081 cataggaagg ggagaagtaa cagggtacac atattgacca aatcagggta attttgcatt
    10141 tgtaatttta aaaaatgctt tcttctttta atatactttt ttgtttatct tatttctaat
    10201 actttcccta atctctttct ttcagggcaa taatgataca atgtatcatg cctctttgca
    10261 ccattctaaa gaataacagt gataatttct gggttaaggc aatagcaata tttctgcata
    10321 taaatatttc tgcatataaa ttgtaactga tgtaagaggt ttcatattgc taatagcagc
    10381 tacaatccag ctaccattct gcttttattt tatggttggg ataaggctgg attattctga
    10441 gtccaagcta gg
//
