LOCUS       sequence-335501-        8511 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Co-expresses of WT-ELOA containing Elongin complex in bacteria. The 
            resulting plasmid was used to generate a single expression construct
            encoding all 3Elongin subunits, with a 6-His tag on ELOB.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8511)
  AUTHORS   He Y, Sato S, Tomomori-Sato C, Chen S, Goode ZH, Conaway JW, Conaway
            RC
  TITLE     Elongin functions as a loading factor for Mediator at 
            ATF6alpha-regulated ER stress response genes.
  JOURNAL   Proc Natl Acad Sci U S A. 2021 Sep 28;118(39):e2108751118. doi: 
            10.1073/pnas.2108751118.
  PUBMED    34544872
REFERENCE   2  (bases 1 to 8511)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2021-09-28"; volume: "118(39):e2108751118. 
            doi"; pages: " 10.1073/pnas.2108751118"
COMMENT     Sequence Label: pQ-Elo-HB-C-A(1-772)
FEATURES             Location/Qualifiers
     source          1..8511
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(36..50,51..56,57..73,74..80)
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    56..72
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    88..104
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             123..134
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             147..167
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=7xHis
                     /translation="HHHHHHH"
     protein_bind    complement(571..595)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      617..711
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     promoter        join(740..754,755..760,761..777,778..784)
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    760..776
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    792..808
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             827..838
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     protein_bind    complement(1224..1248)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      1270..1364
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     promoter        join(1393..1407,1408..1413,1414..1430,1431..1437)
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    1413..1429
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    1445..1461
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             1480..1491
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     regulatory      1496..1505
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(3857..3881)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      3903..3997
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             4106..4765
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(4172..4191)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      4830..4916
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(5009..6091)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     6053..6072
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(6092..6169)
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     primer_bind     complement(6253..6275)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    6361..6501
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     6416..6435
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(6516..6533)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6687..7275)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6767..6786)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7446..8237,8238..8306))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8069..8088
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8307..8411)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8479..8497
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ctcgagctta attaacaaca ccatttgtcg agaaatcata aaaaatttat ttgctttgtg
       61 agcggataac aattataata gattcaattg tgagcggata acaatttcac acagaattca
      121 ttaaagagga gaaattaact atgaaacatc accatcacca tcaccatggt gctatggacg
      181 tgttcctcat gatccggcgc cacaagacca ccatcttcac ggacgccaag gagtccagca
      241 cggtgttcga actgaagcgc atcgtcgagg gcatcctcaa gcggcctcct gacgagcagc
      301 ggctgtacaa ggatgaccaa ctcttggatg atggcaagac actgggcgag tgtggcttca
      361 ccagtcaaac agcacggcca caggccccag ccacagtggg gctggccttc cgggcagatg
      421 acacctttga ggccctgtgc atcgagccgt tttccagccc gccagagctg cccgatgtga
      481 tgaagcccca ggactcggga agcagtgcca atgaacaagc cgtgcagtga ggatccagtc
      541 ttcgcatgca tgaagacgcg gccgcctagg acccagcttt cttgtacaaa gtggtaagct
      601 taattagctg agcttggact cctgttgata gatccagtaa tgacctcaga actccatctg
      661 gatttgttca gaacgctcgg ttgccgccgg gcgtttttta ttggtgagaa tccaagtaac
      721 aacaccattt gtcgagaaat cataaaaaat ttatttgctt tgtgagcgga taacaattat
      781 aatagattca attgtgagcg gataacaatt tcacacagaa ttcattaaag aggagaaatt
      841 aactatggat ggagaggaga aaacctatgg tggctgtgaa ggacctgatg ccatgtatgt
      901 caaattgata tcatctgatg gccatgaatt tattgtaaaa agagaacatg cattaacatc
      961 aggcacgata aaagccatgt tgagtggccc aggtcagttt gctgagaacg aaaccaatga
     1021 ggtcaatttt agagagatac cttcacatgt gctatcgaaa gtatgcatgt attttacgta
     1081 caaggttcgc tacactaaca gctccaccga gattcctgaa ttcccaattg cacctgaaat
     1141 tgcactggaa ctgctgatgg ctgcgaactt cttagattgt taaggatcca gtcttcgcat
     1201 gcatgaagac gcggccgcct aggacccagc tttcttgtac aaagtggtaa gcttaattag
     1261 ctgagcttgg actcctgttg atagatccag taatgacctc agaactccat ctggatttgt
     1321 tcagaacgct cggttgccgc cgggcgtttt ttattggtga gaatccaagt aacaacacca
     1381 tttgtcgaga aatcataaaa aatttatttg ctttgtgagc ggataacaat tataatagat
     1441 tcaattgtga gcggataaca atttcacaca gaattcatta aagaggagaa attaaccatg
     1501 gcggcggagt cggcgctcca agttgtggag aagctgcagg cgcgcctggc cgcgaacccg
     1561 gaccctaaga agctattgaa atatttgaag aaactctcca ccctgcctat tacagtagac
     1621 attcttgcgg agactggggt tgggaaaaca gtaaatagct tgcgaaaaca cgagcatgtt
     1681 ggaagctttg ccagggacct agtggcccag tggaagaagc tggttcctgt ggaacgaaat
     1741 gctgagcctg atgaacagga ctttgagaag agcaattccc gaaagcgccc tcgggatgcc
     1801 ctgcagaagg aggaggagat ggagggggac taccaagaaa cctggaaagc cacggggagc
     1861 cgatcctata gccctgacca caggcagaag aaacatagga aactctcgga gctcgagaga
     1921 cctcacaaag tgtctcacgg tcatgagagg agagatgaga gaaagaggtg tcacagaatg
     1981 tcaccaactt actcttcaga ccctgagtct tctgattatg gccatgttca atcccctcca
     2041 tcttgtacca gtcctcatca gatgtacgtc gaccactaca gatccctgga ggaggaccag
     2101 gagcccattg tttcacacca gaagcctggg aaaggccaca gcaatgcctt tcaggacaga
     2161 ctcggggcca gccaagaacg acacctgggt gaaccccatg ggaaaggggt tgtgagtcaa
     2221 aacaaggagc acaaatcttc ccacaaggac aaacgccccg tggatgccaa gagtgatgag
     2281 aaggcctctg tggtgagcag agagaaatca cacaaggccc tctccaaaga ggagaaccga
     2341 aggccaccct caggggacaa tgcaagggag aaaccgccct ctagtggcgt aaagaaagag
     2401 aaggacagag agggcagcag cctgaagaag aagtgtttgc ctccctcaga ggccgcttca
     2461 gacaaccacc tgaaaaagcc aaagcacaga gacccagaga aagccaaatt ggacaaaagc
     2521 aagcaaggtc tggacagctt tgacacagga aaaggagcag gagacctgtt gcccaaggta
     2581 aaagagaagg gttctaacaa cctaaagact ccagaaggga aagtcaaaac taatttggat
     2641 agaaagtcac tgggctccct ccctaaagtt gaggagacag atatggagga tgaattcgag
     2701 cagccaacca tgtcttttga atcctacctc agctatgacc agccccggaa gaaaaagaaa
     2761 aagattgtga aaacttcagc cacggcactt ggagataaag gacttaaaaa aaatgactct
     2821 aaaagcactg gtaaaaactt ggactcagtt cagaaattac ccaaggtgaa caaaaccaag
     2881 tcagagaagc cggctggagc tgatttagcc aagctgagaa aggtgcctga tgtgttgcca
     2941 gtgttgccag acctcccgtt acccgcgata caggccaatt accgtccact gccttccctc
     3001 gagctgatat cctccttcca gccaaagcga aaagcgttct cttcacccca ggaagaagaa
     3061 gaagctggat ttactgggcg cagaatgaat tccaagatgc aggtgtattc tggttccaag
     3121 tgtgcctatc tccctaaaat gatgaccttg caccagcaat gcatccgagt acttaaaaac
     3181 aacatcgatt caatctttga agtgggagga gtcccatact ctgttcttga acccgttttg
     3241 gagaggtgta cacctgatca gctgtatcgc atagaggaat acaatcatgt attaattgaa
     3301 gaaacagatc aattatggaa agttcattgt caccgagact ttaaggaaga aagacccgaa
     3361 gagtatgagt cgtggcgaga gatgtacctg cggcttcagg acgcccgaga gcagcggcta
     3421 cgagtactaa caaagaatat ccagttcgca catgccaata agcccaaagg ccgacaagca
     3481 aagatggcct ttgtcaactc tgtggccaag ccacctcgtg acgtccggag gaggcaggaa
     3541 aagtttggaa cgggaggagc agctgtccct gagaaaatca agatcaagcc agccccgtac
     3601 cccatgggaa gcagccatgc ttccgccagt agcatcagct ttaaccccag ccctgaggag
     3661 ccggcctatg atggcccaag caccagcagt gcccacttgg caccagtggt cagcagcact
     3721 gtttcctatg atcctaggaa acccactgtg aagaaaattg ccccaatgat ggccaagaca
     3781 attaaagctt tcaagaacag attctcccga cgataaggat ccagtcttcg catgcatgaa
     3841 gacgcggccg cctaggaccc agctttcttg tacaaagtgg taagcttaat tagctgagct
     3901 tggactcctg ttgatagatc cagtaatgac ctcagaactc catctggatt tgttcagaac
     3961 gctcggttgc cgccgggcgt tttttattgg tgagaatcca agtaacaaca ccatttaaat
     4021 ggagtggtta caaatggagt ggttacaaat ggagtggtta attaaggcta gcttggcgag
     4081 attttcagga gctaaggaag ctaaaatgga gaaaaaaatc actggatata ccaccgttga
     4141 tatatcccaa tggcatcgta aagaacattt tgaggcattt cagtcagttg ctcaatgtac
     4201 ctataaccag accgttcagc tggatattac ggccttttta aagaccgtaa agaaaaataa
     4261 gcacaagttt tatccggcct ttattcacat tcttgcccgc ctgatgaatg ctcatccgga
     4321 atttcgtatg gcaatgaaag acggtgagct ggtgatatgg gatagtgttc acccttgtta
     4381 caccgttttc catgagcaaa ctgaaacgtt ttcatcgctc tggagtgaat accacgacga
     4441 tttccggcag tttctacaca tatattcgca agatgtggcg tgttacggtg aaaacctggc
     4501 ctatttccct aaagggttta ttgagaatat gtttttcgtc tcagccaatc cctgggtgag
     4561 tttcaccagt tttgatttaa acgtggccaa tatggacaac ttcttcgccc ccgttttcac
     4621 catgggcaaa tattatacgc aaggcgacaa ggtgctgatg ccgctggcga ttcaggttca
     4681 tcatgccgtt tgtgatggct tccatgtcgg cagaatgctt aatgaattac aacagtactg
     4741 cgatgagtgg cagggcgggg cgtaattttt ttaaggcagt tattggtgcc cttaaacgcc
     4801 tggggtaatg actctctagc ttgaggcatc aaataaaacg aaaggctcag tcgaaagact
     4861 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct cctgagtagg acaaatccgc
     4921 cctctagatt acgtgcagtc gatgataagc tgtcaaacat gagaattgtg cctaatgagt
     4981 gagctaactt acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     5041 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     5101 ccagggtggt ttttcttttc accagtgaga cgggcaacag ctgattgccc ttcaccgcct
     5161 ggccctgaga gagttgcagc aagcggtcca cgctggtttg ccccagcagg cgaaaatcct
     5221 gtttgatggt ggttaacggc gggatataac atgagctgtc ttcggtatcg tcgtatccca
     5281 ctaccgagat atccgcacca acgcgcagcc cggactcggt aatggcgcgc attgcgccca
     5341 gcgccatctg atcgttggca accagcatcg cagtgggaac gatgccctca ttcagcattt
     5401 gcatggtttg ttgaaaaccg gacatggcac tccagtcgcc ttcccgttcc gctatcggct
     5461 gaatttgatt gcgagtgaga tatttatgcc agccagccag acgcagacgc gccgagacag
     5521 aacttaatgg gcccgctaac agcgcgattt gctggtgacc caatgcgacc agatgctcca
     5581 cgcccagtcg cgtaccgtct tcatgggaga aaataatact gttgatgggt gtctggtcag
     5641 agacatcaag aaataacgcc ggaacattag tgcaggcagc ttccacagca atggcatcct
     5701 ggtcatccag cggatagtta atgatcagcc cactgacgcg ttgcgcgaga agattgtgca
     5761 ccgccgcttt acaggcttcg acgccgcttc gttctaccat cgacaccacc acgctggcac
     5821 ccagttgatc ggcgcgagat ttaatcgccg cgacaatttg cgacggcgcg tgcagggcca
     5881 gactggaggt ggcaacgcca atcagcaacg actgtttgcc cgccagttgt tgtgccacgc
     5941 ggttgggaat gtaattcagc tccgccatcg ccgcttccac tttttcccgc gttttcgcag
     6001 aaacgtggct ggcctggttc accacgcggg aaacggtctg ataagagaca ccggcatact
     6061 ctgcgacatc gtataacgtt actggtttca cattcaccac cctgaattga ctctcttccg
     6121 ggcgctatca tgccataccg cgaaaggttt tgcaccattc gatggtgtcg gaatttcggg
     6181 cagcgttggg tcctggccac gggtgcgcat gatctagagc tgcctcgcgc gtttcggtga
     6241 tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc
     6301 ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg
     6361 cgcagccatg acccagtcac gtagcgatag cggagtgtat actggcttaa ctatgcggca
     6421 tcagagcaga ttgtactgag agtgcaccac atgcggtgtg aaataccgca cagatgcgta
     6481 aggagaaaat accgcatcag gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     6541 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     6601 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     6661 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     6721 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     6781 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     6841 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     6901 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     6961 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     7021 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     7081 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt
     7141 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     7201 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     7261 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     7321 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     7381 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     7441 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     7501 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     7561 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     7621 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     7681 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     7741 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     7801 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     7861 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     7921 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     7981 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     8041 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     8101 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     8161 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     8221 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     8281 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     8341 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     8401 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc
     8461 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtcttca c
//
