LOCUS       sequence-335510-        9827 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mTDel_EPACdDEPCD_cp173Ven(ST)_Ven(ST) biosensor to measure realtime 
            changes in FRET reflecting fluctuations in cAMP levels in living 
            cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9827)
  AUTHORS   Klarenbeek JB, Goedhart J, Hink MA, Gadella TW, Jalink K
  TITLE     A mTurquoise-based cAMP sensor for both FLIM and ratiometric 
            read-out has improved dynamic range.
  JOURNAL   PLoS One. 2011 Apr 29;6(4):e19170. doi: 
            10.1371/journal.pone.0019170.
  PUBMED    21559477
REFERENCE   2  (bases 1 to 9827)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011-04-29"; volume: "6(4):e19170. doi"; pages: " 
            10.1371/journal.pone.0019170"
COMMENT     Sequence Label: Epac-S-H74
FEATURES             Location/Qualifiers
     source          1..9827
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     7..26
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7..25
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      42..51
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      46..55
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(60..62,63..65,66..578)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISDNVYITADKQKNGIK
                     ANFKIRHNIE"
     CDS             2964..3698
                     /codon_start=1
                     /product="circularly permuted form of the Venus yellow 
                     fluorescent protein (Nagai et al., 2004)"
                     /label=cp173Venus
                     /note="efficient FRET acceptor for CFP"
                     /translation="MDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRD
                     HMVLLEFVTAAGITLGMDELYKGGSGGMVSKGEELFTGVVPILVELDGDVNGHKFSVSG
                     EGEGDATYGKLTLKLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPE
                     GYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
                     VYITADKQKNGIKANFKIRHNIE"
     CDS             join(3756..3758,3759..3761,3762..4472)
                     /codon_start=1
                     /product="yellow fluorescent protein (YFP) with fast and 
                     efficient maturation (Nagai et al., 2002)"
                     /label=Venus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     promoter        complement(4524..4542)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4525..4542)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4562..4579)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4568..4792
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4838..5266
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4925..4944)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5135..5156
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5275..5295)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5280..5609
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5460..5595
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5522..5541
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5676..6470
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5730..5749)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6340..6359
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6644..6765
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6681..6700)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6735..6754
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6814..6830)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6814..6830)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6827..6849)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6838..6854
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6862..6868,6869..6886,6887..6892))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6907..6928
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7045..7062)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7216..7804)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7296..7315)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7975..8766,8767..8835))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8598..8617
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8836..8940)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9015..9034)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        9206..9585
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9586..9789
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     9740..9760
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 cgaaattaat acgactcact atagggagac ccaagcttgc cgccaccatg gcggccgcaa
       61 tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg
      121 gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg
      181 gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc
      241 tcgtgaccac cctgtcctgg ggcgtgcagt gcttcgcccg ctaccccgac cacatgaagc
      301 agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct
      361 tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg
      421 tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca
      481 agctggagta caactacatc agcgacaacg tctatatcac cgccgacaag cagaagaacg
      541 gcatcaaggc caacttcaag atccgccaca acatcgagga cggcggcgtg cagctcgccg
      601 accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact
      661 acctgagcac ccagtccaag ctgagcaaag accccaacga gaagcgcgat cacatggtcc
      721 tgctggagtt cgtgaccgcc gcccgatatc agcccgtggg aactcatgag atggaggagg
      781 agttggccga agctgtggcc ctgctctccc agcgggggcc tgacgccctg ctcactgtgg
      841 cacttcgaaa gcccccaggt cagcgcacgg atgaagagct ggacctcatc tttgaggagc
      901 tgctgcacat caaggctgtg gcccacctct ccaactcggt gaagcgagaa ttagcggctg
      961 ttctgctctt tgaaccacac agcaaggcag ggaccgtgtt gttcagccag ggggacaagg
     1021 gcacttcgtg gtacattatc tggaagggat ctgtcaacgt ggtgacccat ggcaaggggc
     1081 tggtgaccac cctgcatgag ggagatgatt ttggacagct ggctctggtg aatgatgcac
     1141 cccgggcagc caccatcatc ctgcgagaag acaactgtca tttcctgcgt gtggacaagc
     1201 aggacttcaa ccgtatcatc aaggatgtgg aggcaaagac catgcggctg gaagaacatg
     1261 gcaaagtggt gctggtgctg gagagagcct ctcagggcgc cggcccttcc cgacccccaa
     1321 ccccaggcag gaaccggtat acagtgatgt ctggcacccc agagaagatc ctagagcttc
     1381 tgttggaggc catgggacca gattccagtg ctcatgaccc aacagagaca ttcctcagcg
     1441 acttcctcct gacccacagg gtcttcatgc ccagcgccca actctgcgct gcccttctgc
     1501 accacttcca tgtggagcct gcgggtggca gcgagcagga gcgcagcacc tacgtctgca
     1561 acaagaggca gcagatcttg cggctggtca gccagtgggt ggccctgtat ggctccatgc
     1621 tccacactga ccctgtggcc accagcttcc tccagaaact ctcagacctg gtgggcaggg
     1681 acacccgact cagcaacctg ctgagggagc agtggccaga gaggcggcga tgccacaggt
     1741 tggagaatgg ctgtgggaat gcatctcctc agatgaaggc ccggaacttg cctgtttggc
     1801 tccccaacca ggacgagccc cttcctggca gcagctgtgc catccaagtt ggggataaag
     1861 tcccctatga catctgccgg ccagaccact cagtgttgac cctgcagctg cctgtgacag
     1921 cctccgtgag agaggtgatg gcagcgttgg cccaggagga tggctggacc aaggggcagg
     1981 tgctggtgaa ggtcaattct gcaggtgatg ccattggcct gcagccagat gcccgtggtg
     2041 tggccacatc tctggggctc aatgagcgtc tctttgttgt caacccacag gaagcgcatg
     2101 agctgatccc acaccctgac cagctggggc ccactgtggg ctctgctgag gggctggacc
     2161 tggtgagtgc caaggacctg gcaggccagc tgacggacca cgactggagc ctcttcaaca
     2221 gtatccacca ggtggagctg atccactatg tgctgggccc ccagcatctg cgggatgtca
     2281 ccaccgccaa cctggagcgc ttcatgcgcc gcttcaatga gctgcagtac tgggtggcca
     2341 ccgagctgtg tctctgcccc gtgcccggcc cccgggccca gctgctcagg aagttcatta
     2401 agctggcggc ccacctcaag gagcagaaga atctcaattc cttctttgcc gtcatgtttg
     2461 gcctcagcaa ctcggccatc agccgcctag cccacacctg ggagcggctg ccccacaaag
     2521 tccggaagct gtactccgcc ctcgagaggc tgctggatcc ctcatggaac caccgggtat
     2581 accgactggc cctcgccaag ctctcccctc ctgtcatccc cttcatgccc cttcttctca
     2641 aagacatggc cgccattcat gagggaaacc acacactagt ggagaatctc atcaactttg
     2701 agaagatgag aatgatggcc agagccgcgc ggatgctgca ccactgccga agccacaacc
     2761 ctgtgcctct ctcaccactc agaagccgag tttcccacct ccacgaggac agccaggtgg
     2821 cgaggatttc cacatgctcg gagcagtccc tgagcacccg gagtccagcc agcacctggg
     2881 cttatgtcca gcagctgaag gtcattgaca accagcggga actctcccgc ctctcccgag
     2941 agctggagcc agctagcgag ctcatggacg gcggcgtgca gctcgccgac cactaccagc
     3001 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagctacc
     3061 agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg
     3121 tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagggtggc agcggtggca
     3181 tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg
     3241 gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg
     3301 gcaagctgac cctgaagctg atctgcacca ccggcaagct gcccgtgccc tggcccaccc
     3361 tcgtgaccac cctgggctac ggcctgcagt gcttcgcccg ctaccccgac cacatgaagc
     3421 agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct
     3481 tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg
     3541 tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca
     3601 agctggagta caactacaac agccacaacg tctatatcac cgccgacaag cagaagaacg
     3661 gcatcaaggc caacttcaag atccgccaca acatcgagcc gaattttgtc ttcctgatcg
     3721 gcgccgcagg aatactcttc gtatctagcg agctcatggt gagcaagggc gaggagctgt
     3781 tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca
     3841 gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagctgatct
     3901 gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccactctg ggctacggcc
     3961 tacagtgctt cgcccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca
     4021 tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga
     4081 cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca
     4141 tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc
     4201 acaacgtcta tatcaccgcc gacaagcaga agaacggcat caaggccaac ttcaagatcc
     4261 gccacaacat cgaggacggc ggcgtgcagc tcgccgacca ctaccagcag aacaccccca
     4321 tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagctaccag tccgccctga
     4381 gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg
     4441 ggatcactct cggcatggac gagctgtaca agccgaattc cctcgaggtt aacgcggcgc
     4501 gcctctaatc tagagggccc tattctatag tgtcacctaa atgctagagc tcgctgatca
     4561 gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc
     4621 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg
     4681 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg
     4741 gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag
     4801 gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta
     4861 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     4921 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     4981 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     5041 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     5101 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     5161 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     5221 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg
     5281 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     5341 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa
     5401 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca
     5461 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     5521 ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag
     5581 gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg
     5641 gatctgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg
     5701 caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa
     5761 tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg
     5821 tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt
     5881 ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa
     5941 gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc
     6001 ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg
     6061 ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg
     6121 aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg
     6181 aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg
     6241 gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact
     6301 gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg
     6361 ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc
     6421 ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct
     6481 ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac
     6541 cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat
     6601 cctccagcgc ggggatctca tgctggagtt cttcgcccac cccaacttgt ttattgcagc
     6661 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     6721 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgtatacc
     6781 gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg
     6841 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
     6901 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
     6961 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     7021 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     7081 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     7141 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     7201 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     7261 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     7321 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     7381 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     7441 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     7501 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     7561 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     7621 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     7681 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     7741 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     7801 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     7861 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     7921 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     7981 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     8041 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     8101 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     8161 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     8221 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     8281 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     8341 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     8401 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     8461 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     8521 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     8581 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     8641 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     8701 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     8761 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     8821 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     8881 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     8941 cacatttccc cgaaaagtgc cacctgacgt cgacggatcg ggagatctcc cgatccccta
     9001 tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tctgctccct
     9061 gcttgtgtgt tggaggtcgc tgagtagtgc gcgagcaaaa tttaagctac aacaaggcaa
     9121 ggcttgaccg acaattgcat gaagaatctg cttagggtta ggcgttttgc gctgcttcgc
     9181 gatgtacggg ccagatatac gcgttgacat tgattattga ctagttatta atagtaatca
     9241 attacggggt cattagttca tagcccatat atggagttcc gcgttacata acttacggta
     9301 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat
     9361 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg
     9421 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac
     9481 gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt
     9541 cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg
     9601 cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc
     9661 attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt
     9721 aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
     9781 agcagagctc tctggctaac tagagaaccc actgcttact ggcttat
//
