LOCUS       sequence-335548-        4173 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4173)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 4173)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLC2A9_STOP
FEATURES             Location/Qualifiers
     source          1..4173
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1730..1829)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1846..1865)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1847..1865)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1870..1886)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1870..1886)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1988..2007)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1999..2808
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2066..2085)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2955..3543
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3444..3463
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3697..3714
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3873..3900)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3992..4078)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4127..4149
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4141..4158
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4142..4158
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacacat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccaccatggc caggaagcag
      121 aaccgcaata gcaaggagct gggactggtg ccactgaccg acgatacatc ccacgcagga
      181 ccacctggac ctggaagggc cctgctggag tgcgaccacc tgcgcagcgg agtgcctgga
      241 ggccggagaa ggaaggattg gtcttgtagc ctgctggtgg catccctggc aggagccttc
      301 ggcagctcct ttctctatgg atacaacctg agtgtggtca atgcccccac cccttacatc
      361 aaggccttct ataacgagag ctgggagcgc cggcacggca ggccaatcga ccccgatacc
      421 ctgacactgc tgtggtctgt gaccgtgagc atctttgcca tcggcggcct ggtgggcaca
      481 ctgatcgtga agatgatcgg caaggtgctg ggaaggaagc acaccctgct ggccaacaat
      541 ggcttcgcca tctctgccgc cctgctgatg gcatgcagcc tgcaggcagg agccttcgag
      601 atgctgatcg tgggccggtt tatcatgggc atcgacggag gagtggccct gtccgtgctg
      661 ccaatgtacc tgtccgagat ctctcccaag gagatcagag gctctctggg ccaggtgacc
      721 gccatcttca tctgtatcgg cgtgtttaca ggacagctgc tgggactgcc agagctgctg
      781 ggcaaggagt ctacctggcc ttacctgttc ggcgtgatcg tggtgccagc agtggtgcag
      841 ctgctgagcc tgccttttct gccagactcc ccccggtatc tgctgctgga gaagcacaat
      901 gaggccagag ccgtgaaggc cttccagaca tttctgggca aggccgacgt gagccaggag
      961 gtggaggagg tgctggcaga gagcagggtg cagcggtcca tcagactggt gtctgtgctg
     1021 gagctgctga gggcaccata cgtgagatgg caggtggtga ccgtgatcgt gacaatggcc
     1081 tgctaccagc tgtgcggcct gaacgccatc tggttctata ccaatagcat ctttggcaag
     1141 gccggcatcc cacccgccaa gatcccttat gtgaccctgt ccacaggagg aatcgagaca
     1201 ctggcagccg tgttctctgg cctggtcatc gagcacctgg gcagaaggcc actgctgatc
     1261 ggcggctttg gcctgatggg cctgttcttt ggcaccctga caatcaccct gacactgcag
     1321 gaccacgccc cttgggtgcc atacctgagc atcgtgggca tcctggccat catcgcaagc
     1381 ttctgctccg gaccaggagg catccctttt atcctgaccg gcgagttctt tcagcagtcc
     1441 cagaggccag cagccttcat catcgcagga acagtgaact ggctgtctaa ttttgccgtg
     1501 ggcctgctgt tcccctttat ccagaagagc ctggatacct attgcttcct ggtgtttgcc
     1561 accatctgta tcacaggcgc catctacctg tatttcgtgc tgcccgagac caagaacagg
     1621 acatacgccg agatctccca ggccttttct aagcgcaata aggcctatcc tccagaggag
     1681 aagatcgact ccgccgtgac agatggcaag atcaacggca gaccttaaga cccagctttc
     1741 ttgtacaaag ttggcattat aagaaagcat tgcttatcaa tttgttgcaa cgaacaggtc
     1801 actatcagtc aaaataaaat cattatttgc catccagctg atatccccta tagtgagtcg
     1861 tattacatgg tcatagctgt ttcctggcag ctctggcccg tgtctcaaaa tctctgatgt
     1921 tacattgcac aagataaaaa tatatcatca tgaacaataa aactgtctgc ttacataaac
     1981 agtaatacaa ggggtgttat gagccatatt caacgggaaa cgtcgaggcc gcgattaaat
     2041 tccaacatgg atgctgattt atatgggtat aaatgggctc gcgataatgt cgggcaatca
     2101 ggtgcgacaa tctatcgctt gtatgggaag cccgatgcgc cagagttgtt tctgaaacat
     2161 ggcaaaggta gcgttgccaa tgatgttaca gatgagatgg tcagactaaa ctggctgacg
     2221 gaatttatgc ctcttccgac catcaagcat tttatccgta ctcctgatga tgcatggtta
     2281 ctcaccactg cgatccccgg aaaaacagca ttccaggtat tagaagaata tcctgattca
     2341 ggtgaaaata ttgttgatgc gctggcagtg ttcctgcgcc ggttgcattc gattcctgtt
     2401 tgtaattgtc cttttaacag cgatcgcgta tttcgtctcg ctcaggcgca atcacgaatg
     2461 aataacggtt tggttgatgc gagtgatttt gatgacgagc gtaatggctg gcctgttgaa
     2521 caagtctgga aagaaatgca taaacttttg ccattctcac cggattcagt cgtcactcat
     2581 ggtgatttct cacttgataa ccttattttt gacgagggga aattaatagg ttgtattgat
     2641 gttggacgag tcggaatcgc agaccgatac caggatcttg ccatcctatg gaactgcctc
     2701 ggtgagtttt ctccttcatt acagaaacgg ctttttcaaa aatatggtat tgataatcct
     2761 gatatgaata aattgcagtt tcatttgatg ctcgatgagt ttttctaatc agaattggtt
     2821 aattggttgt aacactggca gagcattacg ctgacttgac gggacggcgc aagctcatga
     2881 ccaaaatccc ttaacgtgag ttacgcgtcg ttccactgag cgtcagaccc cgtagaaaag
     2941 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     3001 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     3061 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     3121 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     3181 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     3241 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     3301 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     3361 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     3421 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     3481 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     3541 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     3601 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     3661 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
     3721 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
     3781 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatacgcgt
     3841 accgctagcc aggaagagtt tgtagaaacg caaaaaggcc atccgtcagg atggccttct
     3901 gcttagtttg atgcctggca gtttatggcg ggcgtcctgc ccgccaccct ccgggccgtt
     3961 gcttcacaac gttcaaatcc gctcccggcg gatttgtcct actcaggaga gcgttcaccg
     4021 acaaacaaca gataaaacga aaggcccagt cttccgactg agcctttcgt tttatttgat
     4081 gcctggcagt tccctactct cgcgttaacg ctagcatgga tgttttccca gtcacgacgt
     4141 tgtaaaacga cggccagtct taagctcggg ccc
//
