LOCUS       sequence-335552-        3984 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3984)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 3984)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLC10A3_STOP
FEATURES             Location/Qualifiers
     source          1..3984
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1541..1640)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1657..1676)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1658..1676)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1681..1697)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1681..1697)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1799..1818)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1810..2619
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1877..1896)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2766..3354
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3255..3274
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3508..3525
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3684..3711)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3803..3889)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3938..3960
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3952..3969
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3953..3969
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacacat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccaccatggt gctgatgcag
      121 gacaagggca gctcccagca gtggcctgga ctgggaggag agggaggagg aaccggacca
      181 ctgtccatgc tgagggccgc cctgctgctg atctctctgc catggggagc acagggaacc
      241 gcatccacat ctctgagcac agcaggagga cacaccgtgc cacctacagg aggccgctac
      301 ctgagcatcg gcgacggatc cgtgatggag ttcgagtttc cagaggattc cgagggcatc
      361 atcgtgatct ctagccagta tccaggacag gcaaacagga ccgcacctgg accaatgctg
      421 agggtgacct ctctggatac agaggtgctg accatcaaga atgtgagcgc catcacatgg
      481 ggcggaggag gaggcttcgt ggtgtccatc cactctggac tggcaggact ggcaccactg
      541 cacatccagc tggtggacgc acacgaggca ccacccaccc tgatcgagga gaggagagat
      601 ttctgcatca aggtgagccc tgcagaggac accccagcca cactgagcgc cgatctggca
      661 cacttttccg agaaccccat cctgtacctg ctgctgcctc tgatcttcgt gaataagtgc
      721 tcctttggct gtaaggtgga gctggaggtg ctgaagggcc tgatgcagtc tccacagcct
      781 atgctgctgg gactgctggg acagttcctg gtcatgcccc tgtacgcctt cctgatggcc
      841 aaggtgttta tgctgcctaa ggccctggcc ctgggcctga tcatcacctg ttcctctcct
      901 ggaggaggag gatcttatct gtttagcctg ctgctgggag gcgacgtgac actggccatc
      961 tctatgacat tcctgagcac cgtggccgcc acaggctttc tgcctctgag ctccgccatc
     1021 tattcccggc tgctgtctat ccacgagacc ctgcacgtgc caatctccaa gatcctgggc
     1081 acactgctgt tcatcgccat ccccatcgcc gtgggcgtgc tgatcaagtc caagctgcct
     1141 aagttttctc agctgctgct gcaggtggtg aagccattca gctttgtgct gctgctggga
     1201 ggactgttcc tggcatacag gatgggcgtg tttatcctgg ccggcatcag actgccaatc
     1261 gtgctggtgg gaatcaccgt gccactggtg ggactgctgg tgggctattg cctggccacc
     1321 tgtctgaagc tgccagtggc acagaggagg acagtgtcta tcgaagtggg agtgcagaac
     1381 agcctgctgg ccctggcaat gctgcagctg agcctgcgga gactgcaggc cgattacgcc
     1441 tcccaggccc ctttcatcgt ggccctgagc ggcacctccg agatgctggc cctggtcatc
     1501 ggccacttca tctattctag cctgtttcca gtgccctaag acccagcttt cttgtacaaa
     1561 gttggcatta taagaaagca ttgcttatca atttgttgca acgaacaggt cactatcagt
     1621 caaaataaaa tcattatttg ccatccagct gatatcccct atagtgagtc gtattacatg
     1681 gtcatagctg tttcctggca gctctggccc gtgtctcaaa atctctgatg ttacattgca
     1741 caagataaaa atatatcatc atgaacaata aaactgtctg cttacataaa cagtaataca
     1801 aggggtgtta tgagccatat tcaacgggaa acgtcgaggc cgcgattaaa ttccaacatg
     1861 gatgctgatt tatatgggta taaatgggct cgcgataatg tcgggcaatc aggtgcgaca
     1921 atctatcgct tgtatgggaa gcccgatgcg ccagagttgt ttctgaaaca tggcaaaggt
     1981 agcgttgcca atgatgttac agatgagatg gtcagactaa actggctgac ggaatttatg
     2041 cctcttccga ccatcaagca ttttatccgt actcctgatg atgcatggtt actcaccact
     2101 gcgatccccg gaaaaacagc attccaggta ttagaagaat atcctgattc aggtgaaaat
     2161 attgttgatg cgctggcagt gttcctgcgc cggttgcatt cgattcctgt ttgtaattgt
     2221 ccttttaaca gcgatcgcgt atttcgtctc gctcaggcgc aatcacgaat gaataacggt
     2281 ttggttgatg cgagtgattt tgatgacgag cgtaatggct ggcctgttga acaagtctgg
     2341 aaagaaatgc ataaactttt gccattctca ccggattcag tcgtcactca tggtgatttc
     2401 tcacttgata accttatttt tgacgagggg aaattaatag gttgtattga tgttggacga
     2461 gtcggaatcg cagaccgata ccaggatctt gccatcctat ggaactgcct cggtgagttt
     2521 tctccttcat tacagaaacg gctttttcaa aaatatggta ttgataatcc tgatatgaat
     2581 aaattgcagt ttcatttgat gctcgatgag tttttctaat cagaattggt taattggttg
     2641 taacactggc agagcattac gctgacttga cgggacggcg caagctcatg accaaaatcc
     2701 cttaacgtga gttacgcgtc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     2761 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     2821 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     2881 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     2941 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     3001 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     3061 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     3121 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     3181 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     3241 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     3301 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     3361 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     3421 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     3481 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     3541 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
     3601 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatacgcg taccgctagc
     3661 caggaagagt ttgtagaaac gcaaaaaggc catccgtcag gatggccttc tgcttagttt
     3721 gatgcctggc agtttatggc gggcgtcctg cccgccaccc tccgggccgt tgcttcacaa
     3781 cgttcaaatc cgctcccggc ggatttgtcc tactcaggag agcgttcacc gacaaacaac
     3841 agataaaacg aaaggcccag tcttccgact gagcctttcg ttttatttga tgcctggcag
     3901 ttccctactc tcgcgttaac gctagcatgg atgttttccc agtcacgacg ttgtaaaacg
     3961 acggccagtc ttaagctcgg gccc
//
