LOCUS       sequence-335555-        8085 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Co-expresses of Delta-C-ELOA containing Elongin complex in bacteria.
            The resulting plasmid was used to generate a single expression 
            construct encoding all 3Elongin subunits, with a 6-His tag on ELOB.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8085)
  AUTHORS   He Y, Sato S, Tomomori-Sato C, Chen S, Goode ZH, Conaway JW, Conaway
            RC
  TITLE     Elongin functions as a loading factor for Mediator at 
            ATF6alpha-regulated ER stress response genes.
  JOURNAL   Proc Natl Acad Sci U S A. 2021 Sep 28;118(39):e2108751118. doi: 
            10.1073/pnas.2108751118.
  PUBMED    34544872
REFERENCE   2  (bases 1 to 8085)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2021-09-28"; volume: "118(39):e2108751118. 
            doi"; pages: " 10.1073/pnas.2108751118"
COMMENT     Sequence Label: pQ-Elo-HB-C-A(1-630)
FEATURES             Location/Qualifiers
     source          1..8085
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(36..50,51..56,57..73,74..80)
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    56..72
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    88..104
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             123..134
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             147..167
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=7xHis
                     /translation="HHHHHHH"
     protein_bind    complement(571..595)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      617..711
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     promoter        join(740..754,755..760,761..777,778..784)
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    760..776
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    792..808
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             827..838
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     protein_bind    complement(1224..1248)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      1270..1364
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     promoter        join(1393..1407,1408..1413,1414..1430,1431..1437)
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    1413..1429
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    1445..1461
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             1480..1491
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     regulatory      1496..1505
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(3431..3455)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      3477..3571
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             3680..4339
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(3746..3765)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      4404..4490
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(4583..5665)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     5627..5646
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(5666..5743)
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     primer_bind     complement(5827..5849)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    5935..6075
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     5990..6009
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(6090..6107)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6261..6849)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6341..6360)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7020..7811,7812..7880))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7643..7662
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7881..7985)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8053..8071
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ctcgagctta attaacaaca ccatttgtcg agaaatcata aaaaatttat ttgctttgtg
       61 agcggataac aattataata gattcaattg tgagcggata acaatttcac acagaattca
      121 ttaaagagga gaaattaact atgaaacatc accatcacca tcaccatggt gctatggacg
      181 tgttcctcat gatccggcgc cacaagacca ccatcttcac ggacgccaag gagtccagca
      241 cggtgttcga actgaagcgc atcgtcgagg gcatcctcaa gcggcctcct gacgagcagc
      301 ggctgtacaa ggatgaccaa ctcttggatg atggcaagac actgggcgag tgtggcttca
      361 ccagtcaaac agcacggcca caggccccag ccacagtggg gctggccttc cgggcagatg
      421 acacctttga ggccctgtgc atcgagccgt tttccagccc gccagagctg cccgatgtga
      481 tgaagcccca ggactcggga agcagtgcca atgaacaagc cgtgcagtga ggatccagtc
      541 ttcgcatgca tgaagacgcg gccgcctagg acccagcttt cttgtacaaa gtggtaagct
      601 taattagctg agcttggact cctgttgata gatccagtaa tgacctcaga actccatctg
      661 gatttgttca gaacgctcgg ttgccgccgg gcgtttttta ttggtgagaa tccaagtaac
      721 aacaccattt gtcgagaaat cataaaaaat ttatttgctt tgtgagcgga taacaattat
      781 aatagattca attgtgagcg gataacaatt tcacacagaa ttcattaaag aggagaaatt
      841 aactatggat ggagaggaga aaacctatgg tggctgtgaa ggacctgatg ccatgtatgt
      901 caaattgata tcatctgatg gccatgaatt tattgtaaaa agagaacatg cattaacatc
      961 aggcacgata aaagccatgt tgagtggccc aggtcagttt gctgagaacg aaaccaatga
     1021 ggtcaatttt agagagatac cttcacatgt gctatcgaaa gtatgcatgt attttacgta
     1081 caaggttcgc tacactaaca gctccaccga gattcctgaa ttcccaattg cacctgaaat
     1141 tgcactggaa ctgctgatgg ctgcgaactt cttagattgt taaggatcca gtcttcgcat
     1201 gcatgaagac gcggccgcct aggacccagc tttcttgtac aaagtggtaa gcttaattag
     1261 ctgagcttgg actcctgttg atagatccag taatgacctc agaactccat ctggatttgt
     1321 tcagaacgct cggttgccgc cgggcgtttt ttattggtga gaatccaagt aacaacacca
     1381 tttgtcgaga aatcataaaa aatttatttg ctttgtgagc ggataacaat tataatagat
     1441 tcaattgtga gcggataaca atttcacaca gaattcatta aagaggagaa attaaccatg
     1501 gcggcggagt cggcgctcca agttgtggag aagctgcagg cgcgcctggc cgcgaacccg
     1561 gaccctaaga agctattgaa atatttgaag aaactctcca ccctgcctat tacagtagac
     1621 attcttgcgg agactggggt tgggaaaaca gtaaatagct tgcgaaaaca cgagcatgtt
     1681 ggaagctttg ccagggacct agtggcccag tggaagaagc tggttcctgt ggaacgaaat
     1741 gctgagcctg atgaacagga ctttgagaag agcaattccc gaaagcgccc tcgggatgcc
     1801 ctgcagaagg aggaggagat ggagggggac taccaagaaa cctggaaagc cacggggagc
     1861 cgatcctata gccctgacca caggcagaag aaacatagga aactctcgga gctcgagaga
     1921 cctcacaaag tgtctcacgg tcatgagagg agagatgaga gaaagaggtg tcacagaatg
     1981 tcaccaactt actcttcaga ccctgagtct tctgattatg gccatgttca atcccctcca
     2041 tcttgtacca gtcctcatca gatgtacgtc gaccactaca gatccctgga ggaggaccag
     2101 gagcccattg tttcacacca gaagcctggg aaaggccaca gcaatgcctt tcaggacaga
     2161 ctcggggcca gccaagaacg acacctgggt gaaccccatg ggaaaggggt tgtgagtcaa
     2221 aacaaggagc acaaatcttc ccacaaggac aaacgccccg tggatgccaa gagtgatgag
     2281 aaggcctctg tggtgagcag agagaaatca cacaaggccc tctccaaaga ggagaaccga
     2341 aggccaccct caggggacaa tgcaagggag aaaccgccct ctagtggcgt aaagaaagag
     2401 aaggacagag agggcagcag cctgaagaag aagtgtttgc ctccctcaga ggccgcttca
     2461 gacaaccacc tgaaaaagcc aaagcacaga gacccagaga aagccaaatt ggacaaaagc
     2521 aagcaaggtc tggacagctt tgacacagga aaaggagcag gagacctgtt gcccaaggta
     2581 aaagagaagg gttctaacaa cctaaagact ccagaaggga aagtcaaaac taatttggat
     2641 agaaagtcac tgggctccct ccctaaagtt gaggagacag atatggagga tgaattcgag
     2701 cagccaacca tgtcttttga atcctacctc agctatgacc agccccggaa gaaaaagaaa
     2761 aagattgtga aaacttcagc cacggcactt ggagataaag gacttaaaaa aaatgactct
     2821 aaaagcactg gtaaaaactt ggactcagtt cagaaattac ccaaggtgaa caaaaccaag
     2881 tcagagaagc cggctggagc tgatttagcc aagctgagaa aggtgcctga tgtgttgcca
     2941 gtgttgccag acctcccgtt acccgcgata caggccaatt accgtccact gccttccctc
     3001 gagctgatat cctccttcca gccaaagcga aaagcgttct cttcacccca ggaagaagaa
     3061 gaagctggat ttactgggcg cagaatgaat tccaagatgc aggtgtattc tggttccaag
     3121 tgtgcctatc tccctaaaat gatgaccttg caccagcaat gcatccgagt acttaaaaac
     3181 aacatcgatt caatctttga agtgggagga gtcccatact ctgttcttga acccgttttg
     3241 gagaggtgta cacctgatca gctgtatcgc atagaggaat acaatcatgt attaattgaa
     3301 gaaacagatc aattatggaa agttcattgt caccgagact ttaaggaaga aagacccgaa
     3361 gagtatgagt cgtggcgaga gatgtactaa ggatccagtc ttcgcatgca tgaagacgcg
     3421 gccgcctagg acccagcttt cttgtacaaa gtggtaagct taattagctg agcttggact
     3481 cctgttgata gatccagtaa tgacctcaga actccatctg gatttgttca gaacgctcgg
     3541 ttgccgccgg gcgtttttta ttggtgagaa tccaagtaac aacaccattt aaatggagtg
     3601 gttacaaatg gagtggttac aaatggagtg gttaattaag gctagcttgg cgagattttc
     3661 aggagctaag gaagctaaaa tggagaaaaa aatcactgga tataccaccg ttgatatatc
     3721 ccaatggcat cgtaaagaac attttgaggc atttcagtca gttgctcaat gtacctataa
     3781 ccagaccgtt cagctggata ttacggcctt tttaaagacc gtaaagaaaa ataagcacaa
     3841 gttttatccg gcctttattc acattcttgc ccgcctgatg aatgctcatc cggaatttcg
     3901 tatggcaatg aaagacggtg agctggtgat atgggatagt gttcaccctt gttacaccgt
     3961 tttccatgag caaactgaaa cgttttcatc gctctggagt gaataccacg acgatttccg
     4021 gcagtttcta cacatatatt cgcaagatgt ggcgtgttac ggtgaaaacc tggcctattt
     4081 ccctaaaggg tttattgaga atatgttttt cgtctcagcc aatccctggg tgagtttcac
     4141 cagttttgat ttaaacgtgg ccaatatgga caacttcttc gcccccgttt tcaccatggg
     4201 caaatattat acgcaaggcg acaaggtgct gatgccgctg gcgattcagg ttcatcatgc
     4261 cgtttgtgat ggcttccatg tcggcagaat gcttaatgaa ttacaacagt actgcgatga
     4321 gtggcagggc ggggcgtaat ttttttaagg cagttattgg tgcccttaaa cgcctggggt
     4381 aatgactctc tagcttgagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct
     4441 ttcgttttat ctgttgtttg tcggtgaacg ctctcctgag taggacaaat ccgccctcta
     4501 gattacgtgc agtcgatgat aagctgtcaa acatgagaat tgtgcctaat gagtgagcta
     4561 acttacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     4621 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgccaggg
     4681 tggtttttct tttcaccagt gagacgggca acagctgatt gcccttcacc gcctggccct
     4741 gagagagttg cagcaagcgg tccacgctgg tttgccccag caggcgaaaa tcctgtttga
     4801 tggtggttaa cggcgggata taacatgagc tgtcttcggt atcgtcgtat cccactaccg
     4861 agatatccgc accaacgcgc agcccggact cggtaatggc gcgcattgcg cccagcgcca
     4921 tctgatcgtt ggcaaccagc atcgcagtgg gaacgatgcc ctcattcagc atttgcatgg
     4981 tttgttgaaa accggacatg gcactccagt cgccttcccg ttccgctatc ggctgaattt
     5041 gattgcgagt gagatattta tgccagccag ccagacgcag acgcgccgag acagaactta
     5101 atgggcccgc taacagcgcg atttgctggt gacccaatgc gaccagatgc tccacgccca
     5161 gtcgcgtacc gtcttcatgg gagaaaataa tactgttgat gggtgtctgg tcagagacat
     5221 caagaaataa cgccggaaca ttagtgcagg cagcttccac agcaatggca tcctggtcat
     5281 ccagcggata gttaatgatc agcccactga cgcgttgcgc gagaagattg tgcaccgccg
     5341 ctttacaggc ttcgacgccg cttcgttcta ccatcgacac caccacgctg gcacccagtt
     5401 gatcggcgcg agatttaatc gccgcgacaa tttgcgacgg cgcgtgcagg gccagactgg
     5461 aggtggcaac gccaatcagc aacgactgtt tgcccgccag ttgttgtgcc acgcggttgg
     5521 gaatgtaatt cagctccgcc atcgccgctt ccactttttc ccgcgttttc gcagaaacgt
     5581 ggctggcctg gttcaccacg cgggaaacgg tctgataaga gacaccggca tactctgcga
     5641 catcgtataa cgttactggt ttcacattca ccaccctgaa ttgactctct tccgggcgct
     5701 atcatgccat accgcgaaag gttttgcacc attcgatggt gtcggaattt cgggcagcgt
     5761 tgggtcctgg ccacgggtgc gcatgatcta gagctgcctc gcgcgtttcg gtgatgacgg
     5821 tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc
     5881 cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc
     5941 catgacccag tcacgtagcg atagcggagt gtatactggc ttaactatgc ggcatcagag
     6001 cagattgtac tgagagtgca ccacatgcgg tgtgaaatac cgcacagatg cgtaaggaga
     6061 aaataccgca tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     6121 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     6181 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     6241 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     6301 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     6361 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     6421 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     6481 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     6541 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     6601 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     6661 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc
     6721 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     6781 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     6841 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     6901 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     6961 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     7021 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     7081 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     7141 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     7201 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     7261 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     7321 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     7381 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     7441 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     7501 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     7561 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     7621 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     7681 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     7741 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     7801 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     7861 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     7921 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     7981 ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca
     8041 ttaacctata aaaataggcg tatcacgagg ccctttcgtc ttcac
//
