LOCUS       sequence-33562-e         881 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 881)
  AUTHORS   Takagi J, Petre BM, Walz T, Springer TA
  TITLE     Global conformational rearrangements in integrin extracellular 
            domains in outside-in and inside-out signaling.
  JOURNAL   Cell. 2002 Sep 6. 110(5):599-11.
  PUBMED    12230977
REFERENCE   2  (bases 1 to 881)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2002 
            Sep 6. 110(5):599-11."
COMMENT     Sequence Label: pEF1-alpha-V
FEATURES             Location/Qualifiers
     source          1..881
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(3..20)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(30..71)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 tcatggtgat ggtgatgatg accggtacgc gtagaatcga gaccgaggag agggttaggg
       61 ataggcttac cttcgaaggg ccctctagac tcgagcggcc gccactgtgc tggatattgt
      121 acctttgtta ttaaaagcaa gaatttggat ggtatcatgt atgtgtttat aaaattctac
      181 catatgagtg attaaaaaaa tccaagttca tgcagttata aagtctttag tattctgaaa
      241 agtaaactgt agtgtaatat gtattaacat taagtaccta tacaaaagac atgattgcta
      301 aggtccaaat ttggactgct ttaaaacaac aacaacaaca acaacaacaa caacaaataa
      361 agttccgtac attaaatcag aaacagtaac tgaaggaggt actattttat ataaagtagt
      421 aaatcaggca ctacctgtct tatatacatt gaataaaaac tatcccttga aattttatta
      481 tttaaatacc taaaaacaca agtcacccta tgttattaaa tgtatgtctg aacttattta
      541 taaggagaag gttgacaaat ataattctca ttttgtggtt aatgccaatt agcactatca
      601 gcagtaaagc cttggatttt tactcctcat gaagaaagtt taaatctata ataaagttgc
      661 taattctagt gggtcaagat gtagcataac ttaaaaactg cagttaagtt tctgagtttc
      721 cttcaccatt ttcatgaggt tgaagctgct ccctttcttg ttcttcttga ggtggccgga
      781 cccgtttaaa aaagcccatc ctgtacatta caaataccaa aacagccagt agcaacaatc
      841 ctgctagaac tgctaaaatg atcacccaca caggcacagg c
//
