LOCUS       sequence-335622-        5210 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  iPTG inducible expression of terpene synthase producing 
            (+)1-10(4)-cadinadiene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5210)
  TITLE     Fox lab plasmids
REFERENCE   2  (bases 1 to 5210)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pTS_A0A0C9VSL7
FEATURES             Location/Qualifiers
     source          1..5210
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      160..246
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      338..365
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        384..475
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(476..544,545..1336)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(694..713)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1507..2095
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1996..2015
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2249..2266
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    2281..2421
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(2347..2366)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2507..2529
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     promoter        2607..2684
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             2685..3767
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(2704..2723)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        join(3999..4004,4005..4021,4022..4028)
                     /label=trc promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    4036..4052
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4041..4063
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     complement(join(5200..5210,1..7))
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
ORIGIN
        1 ttaaatcaga acgcagaagc ggtctgataa aacagaattt gcctggcggc agtagcgcgg
       61 tggtcccacc tgaccccatg ccgaactcag aagtgaaacg ccgtagcgcc gatggtagtg
      121 tggggtctcc ccatgcgaga gtagggaact gccaggcatc aaataaaacg aaaggctcag
      181 tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct cctgagtagg
      241 acaaatccgc cgggagcgga tttgaacgtt gcgaagcaac ggcccggagg gtggcgggca
      301 ggacgcccgc cataaactgc caggcatcaa attaagcaga aggccatcct gacggatggc
      361 ctttttgcgt ttctacaaac tcttttgttt atttttctaa atacattcaa atatgtatcc
      421 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
      481 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
      541 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
      601 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
      661 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt
      721 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
      781 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
      841 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
      901 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
      961 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
     1021 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
     1081 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     1141 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
     1201 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     1261 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     1321 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     1381 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     1441 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     1501 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     1561 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     1621 tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca
     1681 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     1741 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     1801 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     1861 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     1921 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     1981 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     2041 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     2101 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     2161 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     2221 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
     2281 cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatggtgcac
     2341 tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta
     2401 cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg
     2461 gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg
     2521 tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agcagatcaa ttcgcgcgcg
     2581 aaggcgaagc ggcatgcatt tacgttgaca ccatcgaatg gtgcaaaacc tttcgcggta
     2641 tggcatgata gcgcccggaa gagagtcaat tcagggtggt gaatgtgaaa ccagtaacgt
     2701 tatacgatgt cgcagagtat gccggtgtct cttatcagac cgtttcccgc gtggtgaacc
     2761 aggccagcca cgtttctgcg aaaacgcggg aaaaagtgga agcggcgatg gcggagctga
     2821 attacattcc caaccgcgtg gcacaacaac tggcgggcaa acagtcgttg ctgattggcg
     2881 ttgccacctc cagtctggcc ctgcacgcgc cgtcgcaaat tgtcgcggcg attaaatctc
     2941 gcgccgatca actgggtgcc agcgtggtgg tgtcgatggt agaacgaagc ggcgtcgaag
     3001 cctgtaaagc ggcggtgcac aatcttctcg cgcaacgcgt cagtgggctg atcattaact
     3061 atccgctgga tgaccaggat gccattgctg tggaagctgc ctgcactaat gttccggcgt
     3121 tatttcttga tgtctctgac cagacaccca tcaacagtat tattttctcc catgaagacg
     3181 gtacgcgact gggcgtggag catctggtcg cattgggtca ccagcaaatc gcgctgttag
     3241 cgggcccatt aagttctgtc tcggcgcgtc tgcgtctggc tggctggcat aaatatctca
     3301 ctcgcaatca aattcagccg atagcggaac gggaaggcga ctggagtgcc atgtccggtt
     3361 ttcaacaaac catgcaaatg ctgaatgagg gcatcgttcc cactgcgatg ctggttgcca
     3421 acgatcagat ggcgctgggc gcaatgcgcg ccattaccga gtccgggctg cgcgttggtg
     3481 cggatatctc ggtagtggga tacgacgata ccgaagacag ctcatgttat atcccgccgt
     3541 taaccaccat caaacaggat tttcgcctgc tggggcaaac cagcgtggac cgcttgctgc
     3601 aactctctca gggccaggcg gtgaagggca atcagctgtt gcccgtctca ctggtgaaaa
     3661 gaaaaaccac cctggcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
     3721 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
     3781 aatgtaagtt agcgcgaatt gatctggttt gacagcttat catcgactgc acggtgcacc
     3841 aatgcttctg gcgtcaggca gccatcggaa gctgtggtat ggctgtgcag gtcgtaaatc
     3901 actgcataat tcgtgtcgct caaggcgcac tcccgttctg gataatgttt tttgcgccga
     3961 catcataacg gttctggcaa atattctgaa atgagctgtt gacaattaat catccggctc
     4021 gtataatgtg tggaattgtg agcggataac aatttcacac aggaaacaga ccatgtcgac
     4081 tccatccgga tttttcattc cggatctgtg ggctcactgt ccgtacccgg ctatttacca
     4141 tccgaacggt gatgcggtgg ttagcgaatc ggatgaatgg atcctgagcc gctgcacagg
     4201 cattagcccg agcctgcgcc gtgaacttcc tcatctgatg tcgggccaac tggcggcctt
     4261 ttgttacaat tcatgctccg atgaacgcct gcgtttagtg tgtgacttta tgatcaccct
     4321 tttcttgatg gacgatcttt ccgacgaatt taaaaccaaa gagacagaag ttctgatgga
     4381 tattgtgatg aatgcgatgg aggaaccaaa cttctatcgt ccggggatta cgaatggaga
     4441 aaaacacccg gaagtggagc cggatgccag caaactgtcg cgcgactact gggctcgtat
     4501 gcgtgcgggt gcggggcctg aagtccagcg tcgtatcatt agcggggtaa aagagtacct
     4561 gattgcagtc cgccaacagg ccgatgatcg ccgcactggc aacattccag agctggaaaa
     4621 atatatcgaa catcgtcgta tgagttctgg ctgccgccct ctgtttcata tcatcgaata
     4681 ttcactggat tttcagctgc cggaagaagt cgtcaatcat ccactcatgg tccgtatgat
     4741 ggattgcgtt aatgactggg ttgctttttc caacgatatt tttagtttta aggtggaata
     4801 ttctcgtgga gacaaacata atatggtcgc agtcatgatg gaacgctaca aattgaacat
     4861 tcaaagcagc ttcgatcgtg ttgcagcgct gtgtatcgaa gcgatgaaga attaccgtga
     4921 atgcaaagcg gaatttccat cttttggccc ggaagttgat gcacagattg aagcttattg
     4981 ctatgtgatg cagagctgga tgtctggttc gctggcgtgg agttttgtga ccccgcgcta
     5041 tcttggtcca cgtcgtcatg aaattcgtaa aacgctgtgg gtcgatctga ccaatcagcg
     5101 cctgcgctcc gcgaatggcg cgtgatcccg gggatcctct agagtcgacc tgcaggcatg
     5161 caagcttggc tgttttggcg gatgagagaa gattttcagc ctgatacaga
//
