LOCUS       sequence-335623-        7461 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  iPTG inducible expression of taxadiene synthase and geranylgeranyl 
            diphosphate synthase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7461)
  TITLE     Fox lab plasmids
REFERENCE   2  (bases 1 to 7461)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pTS_TXS
FEATURES             Location/Qualifiers
     source          1..7461
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(166..171,172..188,189..195)
                     /label=trc promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    203..219
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     208..230
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     complement(3618..3635)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      3788..3874
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3966..3993
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        4012..4103
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4104..4172,4173..4964)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4322..4341)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5135..5723
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5624..5643
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5877..5894
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    5909..6049
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(5975..5994)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6135..6157
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     promoter        6235..6312
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             6313..7395
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(6332..6351)
                     /label=LacI-R
                     /note="LacI, reverse primer"
ORIGIN
        1 gtgcaccaat gcttctggcg tcaggcagcc atcggaagct gtggtatggc tgtgcaggtc
       61 gtaaatcact gcataattcg tgtcgctcaa ggcgcactcc cgttctggat aatgtttttt
      121 gcgccgacat cataacggtt ctggcaaata ttctgaaatg agctgttgac aattaatcat
      181 ccggctcgta taatgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagacca
      241 tgagcagcag cactggcact agcaaggtgg tttccgagac ttccagtacc attgtggatg
      301 atatccctcg actctccgcc aattatcatg gcgatctgtg gcaccacaat gttatacaaa
      361 ctctggagac accgtttcgt gagagttcta cttaccaaga acgggcagat gagctggttg
      421 tgaaaattaa agatatgttc aatgcgctcg gagacggaga tatcagtccg tctgcatacg
      481 acactgcgtg ggtggcgagg ctggcgacca tttcctctga tggatctgag aagccacggt
      541 ttcctcaggc cctcaactgg gttttcaaca accagctcca ggatggatcg tggggtatcg
      601 aatcgcactt tagtttatgc gatcgattgc ttaacacgac caattctgtt atcgccctct
      661 cggtttggaa aacagggcac agccaagtac aacaaggtgc tgagtttatt gcagagaatc
      721 taagattact caatgaggaa gatgagttgt ccccggattt ccaaataatc tttcctgctc
      781 tgctgcaaaa ggcaaaagcg ttggggatca atcttcctta cgatcttcca tttatcaaat
      841 atttgtcgac aacacgggaa gccaggctta cagatgtttc tgcggcagca gacaatattc
      901 cagccaacat gttgaatgcg ttggaaggac tcgaggaagt tattgactgg aacaagatta
      961 tgaggtttca aagtaaagat ggatctttcc tgagctcccc tgcctccact gcctgtgtac
     1021 tgatgaatac aggggacgaa aaatgtttca cttttctcaa caatctgctc gacaaattcg
     1081 gcggctgcgt gccctgtatg tattccatcg atctgctgga acgcctttcg ctggttgata
     1141 acattgagca tctcggaatc ggtcgccatt tcaaacaaga aatcaaagga gctcttgatt
     1201 atgtctacag acattggagt gaaaggggca tcggttgggg cagagacagc cttgttccag
     1261 atctcaacac cacagccctc ggcctgcgaa ctcttcgcat gcacggatac aatgtttctt
     1321 cagacgtttt gaataatttc aaagatgaaa acgggcggtt cttctcctct gcgggccaaa
     1381 cccatgtcga attgagaagc gtggtgaatc ttttcagagc ttccgacctt gcatttcctg
     1441 acgaaagagc tatggacgat gctagaaaat ttgcagaacc atatcttaga gaggcactgg
     1501 caacgaaaat ctcaaccaat acaaaactat tcaaagagat tgagtacgtg gtggagtacc
     1561 cttggcacat gagtatccca cgcttagaag ccaggagtta tattgattca tacgacgaca
     1621 attatgtatg gcagaggaag actctatata gaatgccatc tttgagtaat tcaaaatgtt
     1681 tagaattggc aaaattggac ttcaatatcg tacaatcttt gcatcaagag gagttgaagc
     1741 ttctaacaag atggtggaag gaatccggca tggcagatat aaatttcact cgacaccgag
     1801 tggcggaggt ttatttttca tcagctacat ttgaacccga atattctgcc actagaattg
     1861 ccttcacaaa aattggttgt ttacaagtcc tttttgatga tatggctgac atctttgcaa
     1921 cactagatga attgaaaagt ttcactgagg gagtaaagag atgggataca tctttgctac
     1981 atgagattcc agagtgtatg caaacttgct ttaaagtttg gttcaaatta atggaagaag
     2041 taaataatga tgtggttaag gtacaaggac gtgacatgct cgctcacata agaaaaccct
     2101 gggagttgta cttcaattgt tatgtacaag aaagggagtg gcttgaagcc gggtatatac
     2161 caacttttga agagtactta aagacttatg ctatatcagt aggccttgga ccgtgtaccc
     2221 tacaaccaat actactaatg ggtgagcttg tgaaagatga tgttgttgag aaagtgcact
     2281 atccctcaaa tatgtttgag cttgtatcct tgagctggcg actaacaaac gacaccaaaa
     2341 catatcaggc tgaaaaggct cgaggacaac aagcctcagg catagcatgc tatatgaagg
     2401 ataatccagg agcaactgag gaagatgcca ttaagcacat ttgtcgtgtt gttgatcggg
     2461 ccttgaaaga agcaagcttt gaatatttca aaccatccaa tgatatccca atgggttgca
     2521 agtcctttat ttttaacctt agattgtgtg tccaaatctt ttacaagttt atagatgggt
     2581 acggaatcgc caatgaggag attaaggact atataagaaa agtttatatt gatccaattc
     2641 aagtatgaga gctcagaagg agatatacat atgtttgatt tcaatgaata tatgaaaagt
     2701 aaggctgttg cggtagacgc ggctctggat aaagcgattc cgctggaata tcccgagaag
     2761 attcacgaat cgatgcgcta ctccctgtta gcaggaggga aacgcgttcg tccggcatta
     2821 tgcatcgcgg cctgtgaact cgtcggcggt tcacaggact tagcaatgcc aactgcttgc
     2881 gcaatggaaa tgattcacac aatgagcctg attcatgatg atttgccttg catggacaac
     2941 gatgactttc ggcgcggtaa acctactaat cataaggttt ttggcgaaga tactgcagtg
     3001 ctggcgggcg atgcgctgct gtcgtttgcc ttcgaacata tcgccgtcgc gacctcgaaa
     3061 accgtcccgt cggaccgtac gcttcgcgtg atttccgagc tgggaaagac catcggctct
     3121 caaggactcg tgggtggtca ggtagttgat atcacgtctg agggtgacgc gaacgtggac
     3181 ctgaaaaccc tggagtggat ccatattcac aaaacggccg tgctgctgga atgtagcgtg
     3241 gtgtcagggg ggatcttggg gggcgccacg gaggatgaaa tcgcgcgtat tcgtcgttat
     3301 gcccgctgtg ttggactgtt atttcaggtg gtggatgaca tcctggatgt cacaaaatcc
     3361 agcgaagagc ttggcaagac cgcgggcaaa gaccttctga cggataaggc tacatacccg
     3421 aaattgatgg gcttggagaa agccaaggag ttcgcagctg aacttgccac gcgggcgaag
     3481 gaagaactct cttctttcga tcaaatcaaa gccgcgccac tgctgggcct cgccgattac
     3541 attgcgtttc gtcagaactg agtcgacctg caggcatgca agcttggctg ttttggcgga
     3601 tgagagaaga ttttcagcct gatacagatt aaatcagaac gcagaagcgg tctgataaaa
     3661 cagaatttgc ctggcggcag tagcgcggtg gtcccacctg accccatgcc gaactcagaa
     3721 gtgaaacgcc gtagcgccga tggtagtgtg gggtcacccc atgcgagagt agggaactgc
     3781 caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg
     3841 tttgtcggtg aacgctctcc tgagtaggac aaatccgccg ggagcggatt tgaacgttgc
     3901 gaagcaacgg cccggagggt ggcgggcagg acgcccgcca taaactgcca ggcatcaaat
     3961 taagcagaag gccatcctga cggatggcct ttttgcgttt ctacaaactc ttttgtttat
     4021 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     4081 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     4141 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     4201 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     4261 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     4321 tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca
     4381 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     4441 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     4501 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     4561 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     4621 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
     4681 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     4741 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     4801 ctggagccgg tgagcgtggg tcacgcggta tcattgcagc actggggcca gatggtaagc
     4861 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     4921 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     4981 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     5041 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     5101 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     5161 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     5221 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     5281 tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     5341 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     5401 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     5461 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     5521 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     5581 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     5641 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     5701 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     5761 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     5821 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     5881 agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt
     5941 gcggtatttc acaccgcata tggtgcactc tcagtacaat ctgctctgat gccgcatagt
     6001 taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc
     6061 gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca
     6121 agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg
     6181 cgcgaggcag cagatcaatt cgcgcgcgaa ggcgaagcgg catgcattta cgttgacacc
     6241 atcgaatggt gcaaaacctt tcgcggtatg gcatgatagc gcccggaaga gagtcaattc
     6301 agggtggtga atgtgaaacc agtaacgtta tacgatgtcg cagagtatgc cggtgtctct
     6361 tatcagaccg tttcccgcgt ggtgaaccag gccagccacg tttctgcgaa aacgcgggaa
     6421 aaagtggaag cggcgatggc ggagctgaat tacattccca accgcgtggc acaacaactg
     6481 gcgggcaaac agtcgttgct gattggcgtt gccacctcca gtctggccct gcacgcgccg
     6541 tcgcaaattg tcgcggcgat taaatctcgc gccgatcaac tgggtgccag cgtggtggtg
     6601 tcgatggtag aacgaagcgg cgtcgaagcc tgtaaagcgg cggtgcacaa tcttctcgcg
     6661 caacgcgtca gtgggctgat cattaactat ccgctggatg accaggatgc cattgctgtg
     6721 gaagctgcct gcactaatgt tccggcgtta tttcttgatg tctctgacca gacacccatc
     6781 aacagtatta ttttctccca tgaagacggt acgcgactgg gcgtggagca tctggtcgca
     6841 ttgggtcacc agcaaatcgc gctgttagcg ggcccattaa gttctgtctc ggcgcgtctg
     6901 cgtctggctg gctggcataa atatctcact cgcaatcaaa ttcagccgat agcggaacgg
     6961 gaaggcgact ggagtgccat gtccggtttt caacaaacca tgcaaatgct gaatgagggc
     7021 atcgttccca ctgcgatgct ggttgccaac gatcagatgg cgctgggcgc aatgcgcgcc
     7081 attaccgagt ccgggctgcg cgttggtgcg gatatctcgg tagtgggata cgacgatacc
     7141 gaagacagct catgttatat cccgccgtta accaccatca aacaggattt tcgcctgctg
     7201 gggcaaacca gcgtggaccg cttgctgcaa ctctctcagg gccaggcggt gaagggcaat
     7261 cagctgttgc ccgtctcact ggtgaaaaga aaaaccaccc tggcgcccaa tacgcaaacc
     7321 gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg
     7381 gaaagcgggc agtgagcgca acgcaattaa tgtaagttag cgcgaattga tctggtttga
     7441 cagcttatca tcgactgcac g
//
