LOCUS       sequence-335629-        3519 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For light regulated release of transferrin receptor (TfR) from the 
            endoplasmic reticulum. Driven by CMV promoter. Fused to oxidation 
            resistant version of GFP (moxGFP)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3519)
  AUTHORS   Bourke AM, Schwartz SL, Bowen AB, Kleinjan MS, Winborn CS, Kareemo 
            DJ, Gutnick A, Schwarz TL, Kennedy MJ
  TITLE     zapERtrap: A light-regulated ER release system reveals unexpected 
            neuronal trafficking pathways.
  JOURNAL   J Cell Biol. 2021 Sep 6;220(9). pii: 212461. doi: 
            10.1083/jcb.202103186. Epub 2021 Jul 9.
  PUBMED    34241635
REFERENCE   2  (bases 1 to 3519)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2021-09-6"; volume: "220(9). pii: 212461. doi"; 
            pages: " 10.1083/jcb.202103186. Epub 2021 Jul 9"
COMMENT     Sequence Label: TfR-moxGFP-DHFR
FEATURES             Location/Qualifiers
     source          1..3519
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(940..951)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     regulatory      2296..2305
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2302..2304,2305..2307,2308..3015)
                     /codon_start=1
                     /product="monomeric GFP variant optimized for use in 
                     oxidizing environments such as the secretory pathway 
                     (Costantini et al., 2015)"
                     /label=moxGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFISTTGKLPVPWPTLVTTLTYGVQSFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIK
                     ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELY"
     primer_bind     complement(2347..2368)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             3043..3519
                     /codon_start=1
                     /gene="folA"
                     /product="E. coli dihydrofolate reductase"
                     /label=ecDHFR
                     /translation="ISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGRHT
                     WESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQFLP
                     KAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
ORIGIN
        1 atgatggatc aagctagatc agcattctct aacttgtttg gtggagaacc attgtcatat
       61 acccggttca gcctggctcg gcaagtagat ggcgataaca gtcatgtgga gatgaaactt
      121 gctgtagatg aagaagaaaa tgctgacaat aacacaaagg ccaatgtcac aaaaccaaaa
      181 aggtgtagtg gaagtatctg ctatgggact attgctgtga tcgtcttttt cttgattgga
      241 tttatgattg gctacttggg ctattgtaaa ggggtagaac caaaaactga gtgtgagaga
      301 ctggcaggaa ccgagtctcc agtgagggag gagccaggag aggacttccc tgcagcacgt
      361 cgcttatatt gggatgacct gaagagaaag ttgtcggaga aactggacag cacagacttc
      421 accggcacca tcaagctgct gaatgaaaat tcatatgtcc ctcgtgaggc tggatctcaa
      481 aaagatgaaa atcttgcgtt gtatgttgaa aatcaatttc gtgaatttaa actcagcaaa
      541 gtctggcgtg atcaacattt tgttaagatt caggtcaaag acagcgctca aaactcggtg
      601 atcatagttg ataagaacgg tagacttgtt tacctggtgg agaatcctgg gggttatgtg
      661 gcgtatagta aggctgcaac agttactggt aaactggtcc atgctaattt tggtactaaa
      721 aaagattttg aggatttata cactcctgtg aatggatcta tagtgattgt cagagcaggg
      781 aaaatcacct ttgcagaaaa ggttgcaaat gctgaaagct taaatgcaat tggtgtgttg
      841 atatacatgg accagactaa atttcccatt gttaacgcag aactttcatt ctttggacat
      901 gctcatctgg ggacaggtga cccttacaca cctggattcc cttccttcaa tcacactcag
      961 tttccaccat ctcggtcatc aggattgcct aatatacctg tccagacaat ctccagagct
     1021 gctgcagaaa agctgtttgg gaatatggaa ggagactgtc cctctgactg gaaaacagac
     1081 tctacatgta ggatggtaac ctcagaaagc aagaatgtga agctcactgt gagcaatgtg
     1141 ctgaaagaga taaaaattct taacatcttt ggagttatta aaggctttgt agaaccagat
     1201 cactatgttg tagttggggc ccagagagat gcatggggcc ctggagctgc aaaatccggt
     1261 gtaggcacag ctctcctatt gaaacttgcc cagatgttct cagatatggt cttaaaagat
     1321 gggtttcagc ccagcagaag cattatcttt gccagttgga gtgctggaga ctttggatcg
     1381 gttggtgcca ctgaatggct agagggatac ctttcgtccc tgcatttaaa ggctttcact
     1441 tatattaatc tggataaagc ggttcttggt accagcaact tcaaggtttc tgccagccca
     1501 ctgttgtata cgcttattga gaaaacaatg caaaatgtga agcatccggt tactgggcaa
     1561 tttctatatc aggacagcaa ctgggccagc aaagttgaga aactcacttt agacaatgct
     1621 gctttccctt tccttgcata ttctggaatc ccagcagttt ctttctgttt ttgcgaggac
     1681 acagattatc cttatttggg taccaccatg gacacctata aggaactgat tgagaggatt
     1741 cctgagttga acaaagtggc acgagcagct gcagaggtcg ctggtcagtt cgtgattaaa
     1801 ctaacccatg atgttgaatt gaacctggac tatgagaggt acaacagcca actgctttca
     1861 tttgtgaggg atctgaacca atacagagca gacataaagg aaatgggcct gagtttacag
     1921 tggctgtatt ctgctcgtgg agacttcttc cgtgctactt ccagactaac aacagatttc
     1981 gggaatgctg agaaaacaga cagatttgtc atgaagaaac tcaatgatcg tgtcatgaga
     2041 gtggagtatc acttcctctc tccctacgta tctccaaaag agtctccttt ccgacatgtc
     2101 ttctggggct ccggctctca cacgctgcca gctttactgg agaacttgaa actgcgtaaa
     2161 caaaataacg gtgcttttaa tgaaacgctg ttcagaaacc agttggctct agctacttgg
     2221 actattcagg gagctgcaaa tgccctctct ggtgacgttt gggacattga caatgagttt
     2281 ggggatccac cggtcgccac catggtgtcc aagggcgagg agctgttcac cggggtggtg
     2341 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttctccgt gcggggcgag
     2401 ggcgagggcg atgccaccaa cggcaagctg accctgaagt tcatcagcac caccggcaag
     2461 ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca gagcttctcc
     2521 cgctaccccg accacatgaa gcgccacgac ttcttcaaga gcgccatgcc cgaaggctac
     2581 gtccaggagc gcaccatctc cttcaaggac gacggcacct acaagacccg cgccgaggtg
     2641 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag
     2701 gacggcaaca tcctggggca caagctggag tacaacttca actcccacaa cgtctatatc
     2761 accgccgaca agcagaagaa cggcatcaag gccaacttca agatccgcca caacgtggag
     2821 gacggctccg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc
     2881 gtgctgctgc ccgacaacca ctacctgtcc acccagtcca agctgtccaa agaccccaac
     2941 gagaagcgcg atcacatggt ccttctggaa ttcgtgaccg ccgccgggat cactcacggc
     3001 atggacgagc tgtacagtgc tggtagtgct ggtagtgctg gtatcagtct gattgcggcg
     3061 ttagcggtag atcgcgttat cggcatggaa aacgccatgc cgtggaacct gcctgccgat
     3121 ctcgcctggt ttaaacgcaa caccttaaat aaacccgtga ttatgggccg ccatacctgg
     3181 gaatcaatcg gtcgtccgtt gccaggacgc aaaaatatta tcctcagcag tcaaccgggt
     3241 acggacgatc gcgtaacgtg ggtgaagtcg gtggatgaag ccatcgcggc gtgtggtgac
     3301 gtaccagaaa tcatggtgat tggcggcggt cgcgtttatg aacagttctt gccaaaagcg
     3361 caaaaactgt atctgacgca tatcgacgca gaagtggaag gcgacaccca tttcccggat
     3421 tacgagccgg atgactggga atcggtattc agcgaattcc acgatgctga tgcgcagaac
     3481 tctcacagct attgctttga gattctggag cggcggtaa
//
