LOCUS       sequence-335631-        8787 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Co-expresses of NFY complex in bacteria. The resulting plasmid was 
            used to generate a single expression construct encoding the three 
            subunit NF-Y, with untagged NFYA, GST-NFYB, and 6-His-NFYC.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8787)
  AUTHORS   He Y, Sato S, Tomomori-Sato C, Chen S, Goode ZH, Conaway JW, Conaway
            RC
  TITLE     Elongin functions as a loading factor for Mediator at 
            ATF6alpha-regulated ER stress response genes.
  JOURNAL   Proc Natl Acad Sci U S A. 2021 Sep 28;118(39):e2108751118. doi: 
            10.1073/pnas.2108751118.
  PUBMED    34544872
REFERENCE   2  (bases 1 to 8787)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2021-09-28"; volume: "118(39):e2108751118. 
            doi"; pages: " 10.1073/pnas.2108751118"
COMMENT     Sequence Label: pQ-NFY-A-GB-HC
FEATURES             Location/Qualifiers
     source          1..8787
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(36..50,51..56,57..73,74..80)
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    56..72
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    88..104
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             123..134
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     protein_bind    complement(1105..1129)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      1151..1245
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     promoter        join(1274..1288,1289..1294,1295..1311,1312..1318)
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    1294..1310
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    1326..1342
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             1361..1372
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             1379..2032
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     1990..2012
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     CDS             2051..2074
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     protein_bind    complement(2745..2769)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      2791..2885
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     promoter        join(2914..2928,2929..2934,2935..2951,2952..2958)
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    2934..2950
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    2966..2982
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             3001..3012
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             3025..3045
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=7xHis
                     /translation="HHHHHHH"
     CDS             3055..3078
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     protein_bind    complement(4133..4157)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      4179..4273
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             4382..5041
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(4448..4467)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      5106..5192
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(5285..6367)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     6329..6348
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(6368..6445)
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     primer_bind     complement(6529..6551)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    6637..6777
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     6692..6711
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(6792..6809)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6963..7551)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7043..7062)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7722..8513,8514..8582))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8345..8364
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8583..8687)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8755..8773
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ctcgagctta attaacaaca ccatttgtcg agaaatcata aaaaatttat ttgctttgtg
       61 agcggataac aattataata gattcaattg tgagcggata acaatttcac acagaattca
      121 ttaaagagga gaaattaact atggagcagt atacagcaaa cagcaatagt tcgacagagc
      181 agattgttgt ccaggcagga cagattcagc agcaggtcca agggcagcca ttaatggtgc
      241 aggtcagtgg aggccagcta atcacatcaa ctggccaacc catcatggtc caggctgtcc
      301 ctggtgggca aggtcaaacc atcatgcaag tacctgtttc tggaacacag ggtttgcagc
      361 aaatacagtt ggtcccacct ggacagatcc agatccaggg tggacaggct gtgcaggtgc
      421 agggccagca gggccagacc cagcagatca tcatccagca gccccagacg gctgtcactg
      481 ctggccagac tcagacacag cagcagattg ctgtccaggg acagcaagtg gcacagactg
      541 ctgaagggca gaccatcgtc tatcaaccag ttaatgcaga tggcaccatt ctccagcaag
      601 ttacagtccc tgtttcaggc atgatcacta tcccagcagc cagtttggca ggagcacaga
      661 ttgttcaaac aggagccaat accaacacaa ccagcagtgg gcaagggact gtcactgtga
      721 cactaccagt ggcaggcaat gtggtcaatt caggagggat ggtcatgatg gttcctgggg
      781 ctggctctgt gcctgctatc caaagaatcc ctctacctgg agcagagatg cttgaagaag
      841 agcctctcta cgtgaatgcc aaacaatacc accgtattct taagaggagg caagcccgag
      901 ctaaactaga ggcagaaggg aaaattccaa aggagagaag gaaatacctg catgagtctc
      961 ggcaccgtca tgccatggca cggaagcgtg gtgaaggtgg acgatttttc tctccaaagg
     1021 aaaaggatag tccccatatg caggatccaa accaagccga tgaagaagca atgacacaga
     1081 tcatccgagt gtcctaagcc taggacccag ctttcttgta caaagtggta agcttaatta
     1141 gctgagcttg gactcctgtt gatagatcca gtaatgacct cagaactcca tctggatttg
     1201 ttcagaacgc tcggttgccg ccgggcgttt tttattggtg agaatccaag taacaacacc
     1261 atttgtcgag aaatcataaa aaatttattt gctttgtgag cggataacaa ttataataga
     1321 ttcaattgtg agcggataac aatttcacac agaattcatt aaagaggaga aattaactat
     1381 gtcccctata ctaggttatt ggaaaattaa gggccttgtg caacccactc gacttctttt
     1441 ggaatatctt gaagaaaaat atgaagagca tttgtatgag cgcgatgaag gtgataaatg
     1501 gcgaaacaaa aagtttgaat tgggtttgga gtttcccaat cttccttatt atattgatgg
     1561 tgatgttaaa ttaacacagt ctatggccat catacgttat atagctgaca agcacaacat
     1621 gttgggtggt tgtccaaaag agcgtgcaga gatttcaatg cttgaaggag cggttttgga
     1681 tattagatac ggtgtttcga gaattgcata tagtaaagac tttgaaactc tcaaagttga
     1741 ttttcttagc aagctacctg aaatgctgaa aatgttcgaa gatcgtttat gtcataaaac
     1801 atatttgaat ggtgatcatg taacccatcc tgacttcatg ttgtatgacg ctcttgatgt
     1861 tgttttatac atggacccaa tgtgcctgga tgcgttccca aaattagttt gttttaaaaa
     1921 acgtattgaa gctatcccac aaattgataa gtacttgaaa tccagcaagt atatagcatg
     1981 gcctttgcag ggctggcaag ccacgtttgg tggtggcgac catcctccaa aatcggatct
     2041 gggtgctgca ctggaagttc tgttccaggg gcccctcgag atgacaatgg atggtgacag
     2101 ttctacaaca gatgcttctc aactaggaat ctctgcagac tatattggag gaagtcatta
     2161 tgttatacag cctcatgatg atactgagga cagcatgaat gatcatgaag acacaaatgg
     2221 ttcaaaagaa agtttcagag aacaagatat atatcttcca atagcaaacg tggctaggat
     2281 aatgaaaaat gccatacctc aaacgggaaa gattgcaaaa gatgccaaag aatgtgttca
     2341 agaatgtgta agtgagttca tcagttttat aacatctgaa gcaagtgaaa ggtgccatca
     2401 agagaaacgg aaaacaatca atggagaaga tattctcttt gctatgtcta ctttaggctt
     2461 tgacagttat gtggaacctc tgaaattata ccttcagaaa ttcagagagg ctatgaaagg
     2521 agaaaaggga attggtggag cagtcacagc tacagatgga ctaagtgaag agcttacaga
     2581 ggaggcattt actaaccagt taccagctgg cttaataacc acagacggtc aacaacaaaa
     2641 tgttatggtt tacacaacat catatcaaca gatttctggt gttcagcaaa ttcagttttc
     2701 atgaggatcc agtcttcgca tgcatgaaga cgcggccgcc taggacccag ctttcttgta
     2761 caaagtggta agcttaatta gctgagcttg gactcctgtt gatagatcca gtaatgacct
     2821 cagaactcca tctggatttg ttcagaacgc tcggttgccg ccgggcgttt tttattggtg
     2881 agaatccaag taacaacacc atttgtcgag aaatcataaa aaatttattt gctttgtgag
     2941 cggataacaa ttataataga ttcaattgtg agcggataac aatttcacac agaattcatt
     3001 aaagaggaga aattaactat gaaacatcac catcaccatc accatggtgc tgcactggaa
     3061 gttctgttcc aggggcccca tatgatgtcc acagaaggag gatttggtgg tactagcagc
     3121 agtgatgccc agcaaagcct acagtcgttc tggcctcggg tcatggaaga aatccggaat
     3181 ttaacagtga aagacttccg agtgcaggaa ctcccactgg ctcgtattaa gaagattatg
     3241 aaactggatg aagatgtgaa gatgatcagt gcagaagcgc ctgtactctt tgccaaggca
     3301 gcccagattt ttatcacaga gttgactctt cgagcctgga ttcacacaga agataacaag
     3361 cgccggactc tacagagaaa tgatatcgcc atggcaatta caaaatttga tcagtttgat
     3421 tttctcatcg atattgttcc aagagatgaa ctgaaacctc caaagcgtca ggaggaggtg
     3481 cgccagtctg taactcctgc cgagccagtc cagtactatt tcacgctggc tcagcaaccc
     3541 accgctgtcc aagtccaggg ccagcagcaa ggccagcaga ccaccagctc cacgaccacc
     3601 atccagcctg ggcagatcat catcgcacag cctcagcagg gccagaccac acctgtgaca
     3661 atgcaggttg gagaaggtca gcaggtgcag attgtccagg ctcagccaca gggtcaagcc
     3721 caacaggccc agagtggcac tggacagacc atgcaggtga tgcagcagat catcactaac
     3781 acaggagaga tccagcagat cccggtgcag ctgaatgccg gccagctgca gtatatccgc
     3841 ttagcccagc ctgtatcagg cactcaagtt gtgcagggac agatccagac acttgccacc
     3901 aatgctcaac agattacaca gacagaggtc cagcaaggac agcagcagtt cagccagttc
     3961 acagatggac agcagctcta ccagatccag caagtcacca tgcctgcggg ccaggacctc
     4021 gcccagccca tgttcatcca gtcagccaac cagccctccg acgggcaggc cccccaggtg
     4081 accggcgact gaggatccag tcttcgcatg catgaagacg cggccgccta ggacccagct
     4141 ttcttgtaca aagtggtaag cttaattagc tgagcttgga ctcctgttga tagatccagt
     4201 aatgacctca gaactccatc tggatttgtt cagaacgctc ggttgccgcc gggcgttttt
     4261 tattggtgag aatccaagta acaacaccat ttaaatggag tggttacaaa tggagtggtt
     4321 acaaatggag tggttaatta aggctagctt ggcgagattt tcaggagcta aggaagctaa
     4381 aatggagaaa aaaatcactg gatataccac cgttgatata tcccaatggc atcgtaaaga
     4441 acattttgag gcatttcagt cagttgctca atgtacctat aaccagaccg ttcagctgga
     4501 tattacggcc tttttaaaga ccgtaaagaa aaataagcac aagttttatc cggcctttat
     4561 tcacattctt gcccgcctga tgaatgctca tccggaattt cgtatggcaa tgaaagacgg
     4621 tgagctggtg atatgggata gtgttcaccc ttgttacacc gttttccatg agcaaactga
     4681 aacgttttca tcgctctgga gtgaatacca cgacgatttc cggcagtttc tacacatata
     4741 ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat ttccctaaag ggtttattga
     4801 gaatatgttt ttcgtctcag ccaatccctg ggtgagtttc accagttttg atttaaacgt
     4861 ggccaatatg gacaacttct tcgcccccgt tttcaccatg ggcaaatatt atacgcaagg
     4921 cgacaaggtg ctgatgccgc tggcgattca ggttcatcat gccgtttgtg atggcttcca
     4981 tgtcggcaga atgcttaatg aattacaaca gtactgcgat gagtggcagg gcggggcgta
     5041 atttttttaa ggcagttatt ggtgccctta aacgcctggg gtaatgactc tctagcttga
     5101 ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt
     5161 tgtcggtgaa cgctctcctg agtaggacaa atccgccctc tagattacgt gcagtcgatg
     5221 ataagctgtc aaacatgaga attgtgccta atgagtgagc taacttacat taattgcgtt
     5281 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     5341 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgccag ggtggttttt cttttcacca
     5401 gtgagacggg caacagctga ttgcccttca ccgcctggcc ctgagagagt tgcagcaagc
     5461 ggtccacgct ggtttgcccc agcaggcgaa aatcctgttt gatggtggtt aacggcggga
     5521 tataacatga gctgtcttcg gtatcgtcgt atcccactac cgagatatcc gcaccaacgc
     5581 gcagcccgga ctcggtaatg gcgcgcattg cgcccagcgc catctgatcg ttggcaacca
     5641 gcatcgcagt gggaacgatg ccctcattca gcatttgcat ggtttgttga aaaccggaca
     5701 tggcactcca gtcgccttcc cgttccgcta tcggctgaat ttgattgcga gtgagatatt
     5761 tatgccagcc agccagacgc agacgcgccg agacagaact taatgggccc gctaacagcg
     5821 cgatttgctg gtgacccaat gcgaccagat gctccacgcc cagtcgcgta ccgtcttcat
     5881 gggagaaaat aatactgttg atgggtgtct ggtcagagac atcaagaaat aacgccggaa
     5941 cattagtgca ggcagcttcc acagcaatgg catcctggtc atccagcgga tagttaatga
     6001 tcagcccact gacgcgttgc gcgagaagat tgtgcaccgc cgctttacag gcttcgacgc
     6061 cgcttcgttc taccatcgac accaccacgc tggcacccag ttgatcggcg cgagatttaa
     6121 tcgccgcgac aatttgcgac ggcgcgtgca gggccagact ggaggtggca acgccaatca
     6181 gcaacgactg tttgcccgcc agttgttgtg ccacgcggtt gggaatgtaa ttcagctccg
     6241 ccatcgccgc ttccactttt tcccgcgttt tcgcagaaac gtggctggcc tggttcacca
     6301 cgcgggaaac ggtctgataa gagacaccgg catactctgc gacatcgtat aacgttactg
     6361 gtttcacatt caccaccctg aattgactct cttccgggcg ctatcatgcc ataccgcgaa
     6421 aggttttgca ccattcgatg gtgtcggaat ttcgggcagc gttgggtcct ggccacgggt
     6481 gcgcatgatc tagagctgcc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat
     6541 gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg
     6601 tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca gccatgaccc agtcacgtag
     6661 cgatagcgga gtgtatactg gcttaactat gcggcatcag agcagattgt actgagagtg
     6721 caccacatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc
     6781 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     6841 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     6901 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     6961 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     7021 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     7081 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     7141 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     7201 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     7261 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     7321 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     7381 cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt
     7441 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     7501 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     7561 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     7621 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     7681 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     7741 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     7801 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     7861 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     7921 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     7981 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     8041 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     8101 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     8161 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     8221 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     8281 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     8341 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     8401 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     8461 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     8521 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     8581 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     8641 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     8701 aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg
     8761 cgtatcacga ggccctttcg tcttcac
//
