LOCUS       sequence-335637-        3744 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo level 0 BvCYP76AD1 coding sequence module.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3744)
  AUTHORS   Grutzner R, Schubert R, Horn C, Yang C, Vogt T, Marillonnet S
  TITLE     Engineering Betalain Biosynthesis in Tomato for High Level Betanin 
            Production in Fruits.
  JOURNAL   Front Plant Sci. 2021 Jun 9;12:682443. doi: 
            10.3389/fpls.2021.682443. eCollection 2021.
  PUBMED    34177999
REFERENCE   2  (bases 1 to 3744)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front Plant
            Sci."; date: "2021-06-9"; volume: "12:682443. doi"; pages: " 
            10.3389/fpls.2021.682443. eCollection 2021"
COMMENT     Sequence Label: pAGM1021
FEATURES             Location/Qualifiers
     source          1..3744
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(40..58)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        126..230
                     /gene="bla"
                     /label=AmpR promoter
     CDS             452..1243
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1414..2002
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1903..1922
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2156..2173
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gctttgagac cacgaagtgg ctcttcagtg gacgaaaggg cctcgtgata cgcctatttt
       61 tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa
      121 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
      181 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgcgctcac
      241 gcaactggtc cagaaccttg accgaacgca gcggtggtaa cggcgcagtg gcggttttca
      301 tggcttgtta tgactgtttt tttttggggt acagtctatg cctcgggcat ccaagcagca
      361 agcgcgttac gccgtgggtc gatgtttgat gttatggagc agcaacgatg ttacgcagca
      421 gggcagtcgc cctaaaacaa agttaaacat catgagggaa gcggtgatcg ccgaagtatc
      481 gactcaacta tcagaggtag ttggcgtcat cgagcgccat ctcgaaccga cgttgctggc
      541 cgtacatttg tacggctccg cagtggatgg cggcctgaag ccacacagcg atattgattt
      601 gctggttacg gtgaccgtaa ggcttgatga aacaacgcgg cgagctttga tcaacgacct
      661 tttggaaact tcggcttccc ctggagagag cgagattctc cgcgctgtag aagtcaccat
      721 tgttgtgcac gacgacatca ttccgtggcg ttatccagct aagcgcgaac tgcaatttgg
      781 agaatggcag cgcaatgaca ttcttgcagg tatcttcgag ccagccacga tcgacattga
      841 tctggctatc ttgctgacaa aagcaagaga acatagcgtt gccttggtag gtccagcggc
      901 ggaggaactc tttgatccgg ttcctgaaca ggatctattt gaggcgctaa atgaaacctt
      961 aacgctatgg aactcgccgc ccgactgggc tggcgatgag cgaaatgtag tgcttacgtt
     1021 gtcccgcatt tggtacagcg cagtaaccgg caaaatcgcg ccgaaggatg tcgctgccga
     1081 ctgggcaatg gagcgcctgc cggcccagta tcagcccgtc atacttgaag ctagacaggc
     1141 ttatcttgga caagaagaag atcgcttggc ctcgcgcgca gatcagttgg aagaatttgt
     1201 ccattacgta aaaggcgaga tcaccaaggt agtcggcaaa taactgtcag accaagttta
     1261 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     1321 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     1381 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     1441 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     1501 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     1561 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     1621 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     1681 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     1741 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     1801 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     1861 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     1921 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     1981 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     2041 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
     2101 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
     2161 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
     2221 gccgattcat taatcactct gtggtctcaa atggatcatg caacattagc aatgatacta
     2281 gccatttggt tcatttcttt tcatttcata aaattacttt ttagccaaca aactaccaaa
     2341 cttcttcctc ctggtccaaa accattgcca ataattggta acatcttaga agttggtaaa
     2401 aaaccccatc gttcatttgc taatcttgct aaaattcacg gccctttaat atcgttacgt
     2461 ctaggaagtg taacaactat tgttgtatca tcagcagatg tagctaaaga aatgttctta
     2521 aaaaaagacc accctctttc taaccgtact attcctaatt ctgtcacggc cggtgaccac
     2581 cataaactca ccatgtcgtg gttgcccgtt tcgccgaaat ggcggaattt tcgtaagatt
     2641 acagccgtcc atttgctttc tcctcaaaga cttgatgctt gccaaacctt tcgccatgcc
     2701 aaggtgcaac agctttatga atatgtacaa gaatgtgcac aaaaaggcca agctgttgat
     2761 attggcaaag ctgcatttac tacctccctt aatttgttat ctaaactatt cttttcagtg
     2821 gaattagccc accataaatc acacacttct caagagttta aggaactaat atggaacatt
     2881 atggaagata ttgggaaacc taattatgct gattattttc ctattttagg ctgtgttgat
     2941 ccatcaggta ttcgtcgaag attagcatgt agttttgaca agttgattgc agtttttcag
     3001 ggtataatat gtgaaaggct tgcgcctgat tcttcaacta caacaacaac gacgactgat
     3061 gatgtgctag acgttcttct tcagctgttc aaacaaaatg agctcactat gggcgagatt
     3121 aaccatttgc tcgtcgacat ttttgatgct ggaacagaca ctacatcaag tacttttgag
     3181 tgggtcatga cagagttaat taggaatcct gaaatgatgg aaaaggctca agaagaaatt
     3241 aagcaagtat tgggcaaaga taaacaaatt caggaatcag acattattaa cctaccttac
     3301 ttacaagcca ttatcaaaga aactttgcga ctacatccac caactgtatt tcttttgcct
     3361 cgtaaagccg acactgatgt tgaactatat ggttatattg tgcctaaaga tgcacaaata
     3421 cttgttaact tatgggctat tggaagagat cctaatgcat ggcaaaatgc tgatattttt
     3481 tcgcccgaaa gatttatagg gtgtgaaatt gatgtcaaag gaagagattt tggactctta
     3541 ccttttggag ccggaagaag gatatgtcct gggatgaatt tggccattag aatgttaact
     3601 ttgatgctag ctactttact tcaattcttc aattggaagc ttgaaggaga cataagtcca
     3661 aaagacttag acatggatga gaaatttggg attgcgttac aaaagacaaa gcctttaaaa
     3721 cttattccaa tacctaggta ttga
//
