LOCUS       sequence-335639-        5122 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  White gene CDS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5122)
  AUTHORS   Matinyan N, Karkhanis MS, Gonzalez Y, Jain A, Saltzman A, 
            Malovannaya A, Sarrion-Perdigones A, Dierick HA, Venken KJT
  TITLE     Multiplexed drug-based selection and counterselection genetic 
            manipulations in Drosophila.
  JOURNAL   Cell Rep. 2021 Sep 14;36(11):109700. doi: 
            10.1016/j.celrep.2021.109700.
  PUBMED    34525356
REFERENCE   2  (bases 1 to 5122)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2021-09-14"; volume: "36(11):109700. doi"; pages: " 
            10.1016/j.celrep.2021.109700"
COMMENT     Sequence Label: pWhite_CDS
FEATURES             Location/Qualifiers
     source          1..5122
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     151..170
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     404..421
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    538..559
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(574..579,580..597,598..604)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    612..628
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     617..639
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     636..652
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     636..652
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        670..688
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     670..687
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     regulatory      2889..2898
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      2893..2902
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(2932..2951)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2933..2951)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2958..2975)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2958..2974)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2967..2989)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3115..3570
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3202..3221)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3412..3433
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3648..3752
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3753..3821,3822..4613)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3971..3990)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(4784..5122,1..250)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
       61 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
      121 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
      181 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
      241 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
      301 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
      361 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
      421 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
      481 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
      541 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
      601 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta
      661 cgccaagcta tttaggtgac actatagaat actcaagcta tgcatccaac gcgttgggag
      721 ctctcccata tggtcgacct gcaggcggcc gcgaattcac tagtgattgg gcgatgagtg
      781 gtctcgaagc tcactccttg cgacgagcgc gcaggcgcag agccagatag gccagcacgc
      841 ggaaggacac aatcaggatg gccaggccca cataatccag tggcaggtcg gcggcactga
      901 aattcagtgt ctccaggatg acctttccgc tgctggggca ggtcgtgttg ctgctggtgc
      961 aggagatctc gccgggctcc acatcggccc actgattgat cagcaggccc tcattggcgt
     1021 agcgaaacca gctcaggtag ctcagccact tcaggtacac gggcacgctg ccggagttca
     1081 gaaagaatcc gccgaacagc aggaagggga taatcacggg gggtcccacg ctcagggcca
     1141 tgctggtgct gctggaggcg cagctgatca ggtagccgaa gctggtcgac acgttggcca
     1201 ccagggtcac cagggccagg caattaaaga aatgcagcac tccggcacgc aggccaatca
     1261 tggggtaggc aatggcggta aacaccaggg gcacggtcag gaacagcggc agctcggcaa
     1321 tggtctttcc caggaagtag gtatcgcagc ggtacaggcg gctgcgggcc tcgcgcatga
     1381 acacgggcag ctcgctggtg aacacgttga tggtggcgaa cacgttctgg aacgtcatgt
     1441 tcgtcagaaa cagaaaaatg gctccattga tgttcatcac tccgacctgc gtcagctgct
     1501 gtcccaggaa gatcaggccg atcagaatgg ccaccatcgt ggtctggatc aggcggacct
     1561 tgaccagcag gggctccttc agcacactca gccagctgcg ccacagcacg gcgcgaaact
     1621 gcataaacca ggtagcctta tacgtatagc cgttctcggg ctgctccagg ggcttctcca
     1681 ggttcttcgt ggccagcagc tgctccatgt cgcgggccac cttggagatg gcgaagttat
     1741 cgcagatctt ggcaatgcga tcgcggctct caatctcgcg tcctggcacc acggccagca
     1801 cttgcacata gaagtcggcg gggttgtaat tcgtggggca ctgggcgccc acatagctga
     1861 agaaatccac ggcctcgctt ggggttccca ggaaggccac gcgtccctcg gccatcagca
     1921 gaatcttatc aaacagctcg aacagctccg agctgggctg gtggatcgtc agaatgaccg
     1981 tctttccctt ctggctcagc ttcttcagca cctgcaccac ggagtgggcg gtgaagctat
     2041 ccaggccgct ggtcggctcg tcgcaaatca gcaggggggg atcggtcagg gcctcgctgg
     2101 caaaggccag gcgcttgcgc tctccgccac tcaggccctt cacgcggcct ggcacgccaa
     2161 taatggtgtg ctggcacttg ctcaggctca gctcctggat gacctggtcc acgcgggcca
     2221 cgcgctgacg gtatgtcagg tggcggggca tgcggaccat ggcctgaaag atcagatgct
     2281 cgcgggctgt caggctgccg atgaacagat cgtcctgctg cacgtaggcg cagcgggcct
     2341 gcatctcctt ggcatccacg ggctggccgt tcagcaggcg catgccgctt ggggacacct
     2401 gaatgccctg ggggctgcgg aaggccaggg cgttcagcag cgtggtcttt ccggcgccac
     2461 tacttcccat cacggccagc agctcgccgg ggtaggccac tccgcacaca ttcttcagca
     2521 ggtgcttgcg tggggctggg atatggcgct cattgcagaa cagtccgcgg gtgcggttca
     2581 ccagctgacg ccagccactg cctggctggt tcacggcgcc gaagatatcc atgttatgcc
     2641 aggcgtacgt cagattctcg gccagctgtc cggatccgct tccgctatcc tctggggggc
     2701 tggggggcag cagggttccg taattcttgg cctggccaaa tccctgattg atgcacgact
     2761 ggctggcggc tccgctatcg ccgttgttca ggtgctcggc gcttgggtgc ttggagcctc
     2821 cgcggatcag cagctcctgg tcctcctgtc ccattcgaga ccactcatcg cccaatcgaa
     2881 ttcccgcggc cgccatggcg gccgggagca tgcgacgtcg ggcccaattc gccctatagt
     2941 gagtcgtatt acaattcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc
     3001 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
     3061 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggacgcgc
     3121 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
     3181 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
     3241 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
     3301 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccagcgc
     3361 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
     3421 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga
     3481 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
     3541 attttaacaa aatattaacg cttacaattt cctgatgcgg tattttctcc ttacgcatct
     3601 gtgcggtatt tcacaccgca tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     3661 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     3721 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     3781 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     3841 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     3901 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     3961 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
     4021 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     4081 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     4141 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     4201 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     4261 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     4321 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     4381 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     4441 ctgataaatc tggagccggt gagcgtgggt cgcgcggtat cattgcagca ctggggccag
     4501 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     4561 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     4621 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     4681 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     4741 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     4801 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     4861 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     4921 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     4981 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     5041 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     5101 gaacgggggg ttcgtgcaca ca
//
