LOCUS       sequence-335641-        3720 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo level 0 Db5GT coding sequence module.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3720)
  AUTHORS   Grutzner R, Schubert R, Horn C, Yang C, Vogt T, Marillonnet S
  TITLE     Engineering Betalain Biosynthesis in Tomato for High Level Betanin 
            Production in Fruits.
  JOURNAL   Front Plant Sci. 2021 Jun 9;12:682443. doi: 
            10.3389/fpls.2021.682443. eCollection 2021.
  PUBMED    34177999
REFERENCE   2  (bases 1 to 3720)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front Plant
            Sci."; date: "2021-06-9"; volume: "12:682443. doi"; pages: " 
            10.3389/fpls.2021.682443. eCollection 2021"
COMMENT     Sequence Label: pAGM921
FEATURES             Location/Qualifiers
     source          1..3720
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(40..58)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        126..230
                     /gene="bla"
                     /label=AmpR promoter
     CDS             452..1243
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1414..2002
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1903..1922
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2156..2173
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gctttgagac cacgaagtgg ctcttcagtg gacgaaaggg cctcgtgata cgcctatttt
       61 tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa
      121 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
      181 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgcgctcac
      241 gcaactggtc cagaaccttg accgaacgca gcggtggtaa cggcgcagtg gcggttttca
      301 tggcttgtta tgactgtttt tttttggggt acagtctatg cctcgggcat ccaagcagca
      361 agcgcgttac gccgtgggtc gatgtttgat gttatggagc agcaacgatg ttacgcagca
      421 gggcagtcgc cctaaaacaa agttaaacat catgagggaa gcggtgatcg ccgaagtatc
      481 gactcaacta tcagaggtag ttggcgtcat cgagcgccat ctcgaaccga cgttgctggc
      541 cgtacatttg tacggctccg cagtggatgg cggcctgaag ccacacagcg atattgattt
      601 gctggttacg gtgaccgtaa ggcttgatga aacaacgcgg cgagctttga tcaacgacct
      661 tttggaaact tcggcttccc ctggagagag cgagattctc cgcgctgtag aagtcaccat
      721 tgttgtgcac gacgacatca ttccgtggcg ttatccagct aagcgcgaac tgcaatttgg
      781 agaatggcag cgcaatgaca ttcttgcagg tatcttcgag ccagccacga tcgacattga
      841 tctggctatc ttgctgacaa aagcaagaga acatagcgtt gccttggtag gtccagcggc
      901 ggaggaactc tttgatccgg ttcctgaaca ggatctattt gaggcgctaa atgaaacctt
      961 aacgctatgg aactcgccgc ccgactgggc tggcgatgag cgaaatgtag tgcttacgtt
     1021 gtcccgcatt tggtacagcg cagtaaccgg caaaatcgcg ccgaaggatg tcgctgccga
     1081 ctgggcaatg gagcgcctgc cggcccagta tcagcccgtc atacttgaag ctagacaggc
     1141 ttatcttgga caagaagaag atcgcttggc ctcgcgcgca gatcagttgg aagaatttgt
     1201 ccattacgta aaaggcgaga tcaccaaggt agtcggcaaa taactgtcag accaagttta
     1261 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     1321 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     1381 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     1441 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     1501 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     1561 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     1621 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     1681 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     1741 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     1801 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     1861 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     1921 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     1981 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     2041 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
     2101 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
     2161 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
     2221 gccgattcat taatcactct gtggtctcaa atgggtacac actcaacagc accagatctc
     2281 catgtagttt tcttcccatt cttggctcat ggccacatga tcccgtccct agacatcgcg
     2341 aagctctttg cagctcgcgg tgtcaaaaca accatcatca ccacccctct taacgcatcc
     2401 atgttcacca aagcaataga aaaaaccaga aaaaacacag aaactcagat ggaaattgaa
     2461 gttttcagct tcccgtctga agaagctggt ttgccccttg gatgtgagaa tttggagcaa
     2521 gccatggcta ttggtgcaaa taatgaattc tttaatgctg cgaatttgct gaaagagcag
     2581 ctggagaatt ttctagtgaa aactcgacca aattgccttg tggccgatat gttcttcacc
     2641 tgggcagctg attccacagc caaattcaac atccctaccc tagtttttca tggatttagc
     2701 tttttcgccc aatgtgccaa agaggttatg tggaggtata agccttataa ggccgtgtca
     2761 tccgacacag aggtattttc tcttccattt cttccccatg aggtgaagat gaccagattg
     2821 caggttccag agagtatgag gaaaggtgag gaaactcact tcactaagag aacagaacgc
     2881 atcagggaat tggaacgcaa gagttatggg gtgattgtta acagctttta cgagttggag
     2941 cccgattatg cagactttct taggaaagaa ttggggagaa gggcatggca tataggccct
     3001 gtttcactat gcaataggag catcgaggat aaagctcaac gagggagaca aacttcaatc
     3061 gatgaagatg aatgcttgaa atggcttaac tccaaaaaac ccgattcagt tatctatatc
     3121 tgtttcggaa gcacaggaca cctcatagcc cctcagttac acgagattgc aacggctcta
     3181 gaagcttccg gacaagactt tatctgggcg gtgaggggtg atcatggtca aggaaatagc
     3241 gaagagtggt tgccaccggg atacgaacat agattgcaag ggaaaggctt gataatacga
     3301 gggtgggctc cacaggtgtt gattttggag cacgaagcta caggaggatt tctgactcac
     3361 tgtgggtgga actcggcact tgaagggatc tctgctggtg tgccaatggt tacatggcct
     3421 acttttgccg agcaattcca taacgagcag ttgttgacac agattttgaa ggtcggggtc
     3481 gctgttggat ctaagaagtg gaccctaaag ccttctatcg aagatgtgat aaaagcggaa
     3541 gatatagaga aggcagttag agaagttatg gtgggagagg aaggtgaaga aaggagaagg
     3601 agggcaaaga agttgaagga aatggcatgg agggctattg aagaaggtgg atcatcctac
     3661 tcggatttaa gtgcactgat tgaggaattg aagggttatc atacttcaga gaaagagtag
//
