LOCUS       sequence-335662-        7479 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo level 1 35S promoter-Db5GT-35S terminator transcription unit.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7479)
  AUTHORS   Grutzner R, Schubert R, Horn C, Yang C, Vogt T, Marillonnet S
  TITLE     Engineering Betalain Biosynthesis in Tomato for High Level Betanin 
            Production in Fruits.
  JOURNAL   Front Plant Sci. 2021 Jun 9;12:682443. doi: 
            10.3389/fpls.2021.682443. eCollection 2021.
  PUBMED    34177999
REFERENCE   2  (bases 1 to 7479)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front Plant
            Sci."; date: "2021-06-9"; volume: "12:682443. doi"; pages: " 
            10.3389/fpls.2021.682443. eCollection 2021"
COMMENT     Sequence Label: pAGM7547
FEATURES             Location/Qualifiers
     source          1..7479
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    109..133
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     rep_origin      225..856
                     /label=oriV
                     /note="incP origin of replication"
     promoter        943..1047
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1048..1116,1117..1908)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1266..1285)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2079..2667
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2568..2587
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             3003..4151
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     misc_feature    4307..4331
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        5366..5711
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     5654..5674
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     polyA_signal    join(7306..7479,1)
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
ORIGIN
        1 cgctccatgg gaattcgtaa ttgtcttcac agagtggggc ccactgcatc caccccagta
       61 cattaaaaac gtccgcaatg tgttattaag ttgtctaagc gtcaatttgt ttacaccaca
      121 atatatcctg ccaccagcca gccaacagct ccccgaccgg cagctcggca caaaatcacc
      181 actcgataca ggcagcccat cagtcagatc aggatctcct ttgcgacgct caccgggctg
      241 gttgccctcg ccgctgggct ggcggccgtc tatggccctg caaacgcgcc agaaacgccg
      301 tcgaagccgt gtgcgagaca ccgcggccgc cggcgttgtg gatacctcgc ggaaaacttg
      361 gccctcactg acagatgagg ggcggacgtt gacacttgag gggccgactc acccggcgcg
      421 gcgttgacag atgaggggca ggctcgattt cggccggcga cgtggagctg gccagcctcg
      481 caaatcggcg aaaacgcctg attttacgcg agtttcccac agatgatgtg gacaagcctg
      541 gggataagtg ccctgcggta ttgacacttg aggggcgcga ctactgacag atgaggggcg
      601 cgatccttga cacttgaggg gcagagtgct gacagatgag gggcgcacct attgacattt
      661 gaggggctgt ccacaggcag aaaatccagc atttgcaagg gtttccgccc gtttttcggc
      721 caccgctaac ctgtctttta acctgctttt aaaccaatat ttataaacct tgtttttaac
      781 cagggctgcg ccctgtgcgc gtgaccgcgc acgccgaagg ggggtgcccc cccttctcga
      841 accctcccgg cccgctaacg cgggcctccc atccccccag gggctgcgcc cctcggccgc
      901 gaacggcctc accccaaaaa tggcagcgct ggccaattcg tgcgcggaac ccctatttgt
      961 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ttgataaatg
     1021 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     1081 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     1141 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     1201 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     1261 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
     1321 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     1381 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     1441 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     1501 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     1561 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     1621 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     1681 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     1741 aaatctggag ccggtgagcg tggttctcgc ggtatcattg cagcactggg gccagatggt
     1801 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     1861 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     1921 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     1981 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     2041 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     2101 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     2161 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     2221 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     2281 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     2341 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     2401 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     2461 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     2521 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     2581 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     2641 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     2701 gcagatccta gatgtggcgc aacgatgccg gcgacaagca ggagcgcacc gacttcttcc
     2761 gcatcaagtg ttttggctct caggccgagg cccacggcaa gtatttgggc aaggggtcgc
     2821 tggtattcgt gcagggcaag attcggaata ccaagtacga gaaggacggc cagacggtct
     2881 acgggaccga cttcattgcc gataaggtgg attatctgga caccaaggca ccaggcgggt
     2941 caaatcagga ataagggcac attgccccgg cgtgagtcgg ggcaatcccg caaggagggt
     3001 gaatgaatcg gacgtttgac cggaaggcat acaggcaaga actgatcgac gcggggtttt
     3061 ccgccgagga tgccgaaacc atcgcaagcc gcaccgtcat gcgtgcgccc cgcgaaacct
     3121 tccagtccgt cggctcgatg gtccagcaag ctacggccaa gatcgagcgc gacagcgtgc
     3181 aactggctcc ccctgccctg cccgcgccat cggccgccgt ggagcgttcg cgtcgtcttg
     3241 aacaggaggc ggcaggtttg gcgaagtcga tgaccatcga cacgcgagga actatgacga
     3301 ccaagaagcg aaaaaccgcc ggcgaggacc tggcaaaaca ggtcagcgag gccaagcagg
     3361 ccgcgttgct gaaacacacg aagcagcaga tcaaggaaat gcagctttcc ttgttcgata
     3421 ttgcgccgtg gccggacacg atgcgagcga tgccaaacga cacggcccgc tctgccctgt
     3481 tcaccacgcg caacaagaaa atcccgcgcg aggcgctgca aaacaaggtc attttccacg
     3541 tcaacaagga cgtgaagatc acctacaccg gcgtcgagct gcgggccgac gatgacgaac
     3601 tggtgtggca gcaggtgttg gagtacgcga agcgcacccc tatcggcgag ccgatcacct
     3661 tcacgttcta cgagctttgc caggacctgg gctggtcgat caatggccgg tattacacga
     3721 aggccgagga atgcctgtcg cgcctacagg cgacggcgat gggcttcacg tccgaccgcg
     3781 ttgggcacct ggaatcggtg tcgctgctgc accgcttccg cgtcctggac cgtggcaaga
     3841 aaacgtcccg ttgccaggtc ctgatcgacg aggaaatcgt cgtgctgttt gctggcgacc
     3901 actacacgaa attcatatgg gagaagtacc gcaagctgtc gccgacggcc cgacggatgt
     3961 tcgactattt cagctcgcac cgggagccgt acccgctcaa gctggaaacc ttccgcctca
     4021 tgtgcggatc ggattccacc cgcgtgaaga agtggcgcga gcaggtcggc gaagcctgcg
     4081 aagagttgcg aggcagcggc ctggtggaac acgcctgggt caatgatgac ctggtgcatt
     4141 gcaaacgcta gggccttgtg gggtcagttc cggctggggg ttcagcagcc agcgcctgat
     4201 ctggggaacc ctgtggttgg catgcacata caaatggacg aacggataaa ccttttcacg
     4261 cccttttaaa tatccgatta ttctaataaa cgctcttttc tcttaggttt acccgccaat
     4321 atatcctgtc aaacactgat agtttaaacc acttcgtgca gaagacaatc tgggaggaat
     4381 tccaatccca caaaaatctg agcttaacag cacagttgct cctctcagag cagaatcggg
     4441 tattcaacac cctcatatca actactacgt tgtgtataac ggtccacatg ccggtatata
     4501 cgatgactgg ggttgtacaa aggcggcaac aaacggcgtt cccggagttg cacacaagaa
     4561 atttgccact attacagagg caagagcagc agctgacgcg tacacaacaa gtcagcaaac
     4621 agacaggttg aacttcatcc ccaaaggaga agctcaactc aagcccaaga gctttgctaa
     4681 ggccctaaca agcccaccaa agcaaaaagc ccactggctc acgctaggaa ccaaaaggcc
     4741 cagcagtgat ccagccccaa aagagatctc ctttgccccg gagattacaa tggacgattt
     4801 cctctatctt tacgatctag gaaggaagtt cgaaggtgaa ggtgacgaca ctatgttcac
     4861 cactgataat gagaaggtta gcctcttcaa tttcagaaag aatgctgacc cacagatggt
     4921 tagagaggcc tacgcagcaa gtctcatcaa gacgatctac ccgagtaaca atctccagga
     4981 gatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga ctaattgcat
     5041 caagaacaca gagaaagaca tatttctcaa gatcagaagt actattccag tatggacgat
     5101 tcaaggcttg cttcataaac caaggcaagt aatagagatt ggagtctcta aaaaggtagt
     5161 tcctactgaa tctaaggcca tgcatggagt ctaagattca aatcgaggat ctaacagaac
     5221 tcgccgtcaa gactggcgaa cagttcatac agagtctttt acgactcaat gacaagaaga
     5281 aaatcttcgt caacatggtg gagcacgaca ctctggtcta ctccaaaaat gtcaaagata
     5341 cagtctcaga agatcaaagg gctattgaga cttttcaaca aaggataatt tcgggaaacc
     5401 tcctcggatt ccattgccca gctatctgtc acttcatcga aaggacagta gaaaaggaag
     5461 gtggctccta caaatgccat cattgcgata aaggaaaggc tatcattcaa gatctctctg
     5521 ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa aaagaagagg
     5581 ttccaaccac gtctacaaag caagtggatt gatgtgacat ctccactgac gtaagggatg
     5641 acgcacaatc ccactatcct tcgcaagacc cttcctctat ataaggaagt tcatttcatt
     5701 tggagaggac acgctcgagt ataagagctc atttttacaa caattaccaa caacaacaaa
     5761 caacaaacaa cattacaatt acatttacaa ttatcgatac aatgggtaca cactcaacag
     5821 caccagatct ccatgtagtt ttcttcccat tcttggctca tggccacatg atcccgtccc
     5881 tagacatcgc gaagctcttt gcagctcgcg gtgtcaaaac aaccatcatc accacccctc
     5941 ttaacgcatc catgttcacc aaagcaatag aaaaaaccag aaaaaacaca gaaactcaga
     6001 tggaaattga agttttcagc ttcccgtctg aagaagctgg tttgcccctt ggatgtgaga
     6061 atttggagca agccatggct attggtgcaa ataatgaatt ctttaatgct gcgaatttgc
     6121 tgaaagagca gctggagaat tttctagtga aaactcgacc aaattgcctt gtggccgata
     6181 tgttcttcac ctgggcagct gattccacag ccaaattcaa catccctacc ctagtttttc
     6241 atggatttag ctttttcgcc caatgtgcca aagaggttat gtggaggtat aagccttata
     6301 aggccgtgtc atccgacaca gaggtatttt ctcttccatt tcttccccat gaggtgaaga
     6361 tgaccagatt gcaggttcca gagagtatga ggaaaggtga ggaaactcac ttcactaaga
     6421 gaacagaacg catcagggaa ttggaacgca agagttatgg ggtgattgtt aacagctttt
     6481 acgagttgga gcccgattat gcagactttc ttaggaaaga attggggaga agggcatggc
     6541 atataggccc tgtttcacta tgcaatagga gcatcgagga taaagctcaa cgagggagac
     6601 aaacttcaat cgatgaagat gaatgcttga aatggcttaa ctccaaaaaa cccgattcag
     6661 ttatctatat ctgtttcgga agcacaggac acctcatagc ccctcagtta cacgagattg
     6721 caacggctct agaagcttcc ggacaagact ttatctgggc ggtgaggggt gatcatggtc
     6781 aaggaaatag cgaagagtgg ttgccaccgg gatacgaaca tagattgcaa gggaaaggct
     6841 tgataatacg agggtgggct ccacaggtgt tgattttgga gcacgaagct acaggaggat
     6901 ttctgactca ctgtgggtgg aactcggcac ttgaagggat ctctgctggt gtgccaatgg
     6961 ttacatggcc tacttttgcc gagcaattcc ataacgagca gttgttgaca cagattttga
     7021 aggtcggggt cgctgttgga tctaagaagt ggaccctaaa gccttctatc gaagatgtga
     7081 taaaagcgga agatatagag aaggcagtta gagaagttat ggtgggagag gaaggtgaag
     7141 aaaggagaag gagggcaaag aagttgaagg aaatggcatg gagggctatt gaagaaggtg
     7201 gatcatccta ctcggattta agtgcactga ttgaggaatt gaagggttat catacttcag
     7261 agaaagagta ggcttctcta gctagagtcg atcgacaagc tcgagtttct ccataataat
     7321 gtgtgagtag ttcccagata agggaattag ggttcctata gggtttcgct catgtgttga
     7381 gcatataaga aacccttagt atgtatttgt atttgtaaaa tacttctatc aataaaattt
     7441 ctaattccta aaaccaaaat ccagtactaa aatccagat
//
