LOCUS       sequence-335664-       10728 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  HiLITR transcription factor with full-length TMED3 targeting 
            sequence (ER/Golgi).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10728)
  AUTHORS   Coukos RW, Yao D, Sanchez ML, Strand ET, Olive ME, Udeshi ND, 
            Weissman JS, Carr SA, Bassik MC, Ting AY
  TITLE     An engineered transcriptional reporter of protein localization 
            identifies regulators of mitochondrial and ER membrane protein 
            trafficking in high-throughput CRISPRi screens.
  JOURNAL   eLife. 2021 Aug 20;10. pii: 69142. doi: 10.7554/eLife.69142.
  PUBMED    34414886
REFERENCE   2  (bases 1 to 10728)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife."; 
            date: "2021-08-20"; volume: "10. pii: 69142. doi"; pages: " 
            10.7554/eLife.69142"
COMMENT     Sequence Label: TMED3-hLOV-TEVcs-GAL4bd-VP64-V5
FEATURES             Location/Qualifiers
     source          1..10728
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     146..165
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     399..416
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    533..554
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(569..574,575..592,593..599)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    607..623
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     612..634
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     631..647
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     631..647
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     666..686
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        668..686
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        714..940
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             941..1121
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1168..1293
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1786..2019
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2204..2248
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2423..2923
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     2842..2860
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             2935..3954
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     "
     misc_feature    4011..4128
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4153..5331
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          4384..5322
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     5279..5299
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      5338..5347
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6496..6516
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQM"
     CDS             6553..6990
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="KLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTKR
                     SPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNKD
                     AVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     CDS             7039..7059
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7075..7224
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             7231..7272
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    7294..7882
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7347..7367)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7765..7776)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7954..8187
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8265..8386
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8302..8321)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8356..8375
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8426..8561
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8581..8600)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8582..8600)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8610..8627)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8610..8626)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8619..8641)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8768..9223
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8855..8874)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9065..9086
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9249..9353
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9354..9422,9423..10214)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9572..9591)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(10385..10728,1..245)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
       61 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
      121 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
      181 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
      241 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
      301 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
      361 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
      421 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
      481 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
      541 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt
      601 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca
      661 agcgcgcaat taaccctcac taaagggaac aaaagctgga gctgcaagct taatgtagtc
      721 ttatgcaata ctcttgtagt cttgcaacat ggtaacgatg agttagcaac atgccttaca
      781 aggagagaaa aagcaccgtg catgccgatt ggtggaagta aggtggtacg atcgtgcctt
      841 attaggaagg caacagacgg gtctgacatg gattggacga accactgaat tgccgcattg
      901 cagagatatt gtatttaagt gcctagctcg atacataaac gggtctctct ggttagacca
      961 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     1021 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     1081 atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac
     1141 ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg
     1201 cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga
     1261 ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg
     1321 cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata
     1381 gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca
     1441 gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa
     1501 cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata
     1561 aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc
     1621 gcacagcaag cggccgctga tcttcagacc tggaggagga gatatgaggg acaattggag
     1681 aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag cacccaccaa
     1741 ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag ctttgttcct
     1801 tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc tgacggtaca
     1861 ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga gggctattga
     1921 ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc aggcaagaat
     1981 cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg gttgctctgg
     2041 aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata aatctctgga
     2101 acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca attacacaag
     2161 cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt
     2221 attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa attggctgtg
     2281 gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa tagtttttgc
     2341 tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt ttcagaccca
     2401 cctcccaacc ccgaggggac cattgcgcct tttccaaggc agccctgggt ttgcgcaggg
     2461 acgcggctgc tctgggcgtg gttccgggaa acgcagcggc gccgaccctg ggtctcgcac
     2521 attcttcacg tccgttcgca gcgtcacccg gatcttcgcc gctacccttg tgggcccccc
     2581 ggcgacgctt cctgctccgc ccctaagtcg ggaaggttcc ttgcggttcg cggcgtgccg
     2641 gacgtgacaa acggaagccg cacgtctcac tagtaccctc gcagacggac agcgccaggg
     2701 agcaatggca gcgcgccgac cgcgatgggc tgtggccaat agcggctgct cagcagggcg
     2761 cgccgagagc agcggccggg aaggggcggt gcgggaggcg gggtgtgggg cggtagtgtg
     2821 ggccctgttc ctgcccgcgc ggtgttccgc attctgcaag cctccggagc gcacgtcggc
     2881 agtcggctcc ctcgttgacc gaatcaccga cctctctccc cagggggtac caccatgaaa
     2941 aagcctgaac tcaccgctac ctctgtcgag aagtttctga tcgaaaagtt cgacagcgtc
     3001 tccgacctga tgcagctctc cgagggcgaa gaatctcggg ctttcagctt cgatgtggga
     3061 gggcgtggat atgtcctgcg ggtgaatagc tgcgccgatg gtttctacaa agatcgctat
     3121 gtttatcggc actttgcatc cgccgctctc cctattcccg aagtgcttga cattggggag
     3181 ttcagcgaga gcctgaccta ttgcatctcc cgccgtgcac agggtgtcac cttgcaagac
     3241 ctgcctgaaa ccgaactgcc cgctgttctc cagcccgtcg ccgaggccat ggatgccatc
     3301 gctgccgccg atcttagcca gaccagcggg ttcggcccat tcggacctca aggaatcggt
     3361 caatacacta catggcgcga tttcatctgc gctattgctg atccccatgt gtatcactgg
     3421 caaactgtga tggacgacac cgtcagtgcc tccgtcgccc aggctctcga tgagctgatg
     3481 ctttgggccg aggactgccc cgaagtccgg cacctcgtgc acgccgattt cggctccaac
     3541 aatgtcctga ccgacaatgg ccgcataaca gccgtcattg actggagcga ggccatgttc
     3601 ggggattccc aatacgaggt cgccaacatc ttcttctgga ggccctggtt ggcttgtatg
     3661 gagcagcaga cccgctactt cgagcggagg catcccgagc ttgcaggatc tcctcggctc
     3721 cgggcttata tgctccgcat tggtcttgac caactctatc agagcttggt tgacggcaat
     3781 ttcgatgatg cagcttgggc tcagggtcgc tgcgacgcaa tcgtccggtc cggagccggg
     3841 actgtcgggc gtacacaaat cgcccgcaga agcgctgccg tctggaccga tggctgtgtg
     3901 gaagtgctcg ccgatagtgg aaacagacgc cccagcactc gtcctagggc aaaggatctg
     3961 cagtaatgag aattctagat cttgagacaa atggcagtat tcatccacaa ttttaaaaga
     4021 aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac
     4081 atacaaacta aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac
     4141 agggaccccg ggggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc
     4201 gagaagttgg ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta
     4261 aactgggaaa gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg
     4321 tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca
     4381 caggtaagtg ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc
     4441 gtgccttgaa ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt
     4501 ggaagtgggt gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga
     4561 gttgaggcct ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc
     4621 tgtctcgctg ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg
     4681 ctttttttct ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg
     4741 gtttttgggg ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg
     4801 cggggcctgc gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct
     4861 gctctggtgc ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc
     4921 cggtcggcac cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc
     4981 tcaaaatgaa ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa
     5041 agggcctttc cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc
     5101 aggcacctcg attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg
     5161 ttttatgcga tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg
     5221 cacttgatgt aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc
     5281 aagcctcaga cagtggttca aagttttttt cttccatttc aggtgtcgtg agctagcgcc
     5341 accatgggca gcactgtccc gcgctccgcc tccgtgctgc ttctgctgct gctcctgcgc
     5401 cgggccgagc agccctgcgg ggccgagctc accttcgagc tgccggacaa cgccaagcag
     5461 tgcttccacg aggaggtgga gcagggcgtg aagttctccc tggattacca ggtcatcact
     5521 ggaggccact acgatgttga ctgctatgta gaggaccccc aggggaacac catctacaga
     5581 gaaacgaaga agcagtacga cagcttcacg taccgggctg aagtcaaggg cgtttatcag
     5641 ttttgcttca gtaatgagtt ttccaccttc tctcacaaga ccgtctactt tgactttcaa
     5701 gtgggcgatg agcctcccat tctcccagac atggggaaca gggtcacagc tctcacccag
     5761 atggagtccg cctgcgtgac catccatgag gctctgaaaa cggtgattga ctcccagacg
     5821 cattaccggc tgcgggaggc ccaggaccgg gcccgagcgg aagaccttaa tagccgagtc
     5881 tcttactggt ctgttggcga gacgattgcc ctgttcgtgg tcagcttcag tcaggtgcta
     5941 ctgttgaaaa gcttcttcac agaaaaacga cccatcagca gggcagtcca ctccggttcc
     6001 agtggaggcg gtagctcagg ttcgatcgat gagctggccg agaagctggc cggcctggac
     6061 atcaacggtg gaaccggtga gtttcgggca accacactgg aacggatcga gaaaagcttc
     6121 gtgattactg atccgagact gcctgacaac ccaatcattt ttgtgagcga ttccttcctg
     6181 cagctgacag aatattctcg ggaagagatc ctggggcgca attgccgttt tctgcaggga
     6241 cccgagacag accgtgccac tgttcggaaa atcagagatg ctattgacaa ccagactgaa
     6301 gtgaccgttc agctgatcaa ttataccaag agcggcaaga agttctggaa cgtgttccac
     6361 ctgcagccga tgcgcgatta taagggcgac gtccagtact tcattggcgt gcagctggat
     6421 ggcaccgaac gtcttcatgg cgccgctgag cgtgaggcgg tctgcctggt caaaaagaca
     6481 gcctttcaga ttgctgagaa cctgtacttc cagatgggcg gaggatccgg tagcggagga
     6541 tcaggctcct ctaaactttt gtcctctata gagcaagcct gtgatatatg ccgacttaag
     6601 aaattgaagt gtagcaaaga gaaaccgaag tgtgcgaaat gtttgaaaaa caattgggag
     6661 tgtaggtatt ccccaaaaac taagagatca ccactcacca gggctcattt gactgaggtt
     6721 gagtcacggc tggagagact ggagcagctg ttcctcctga tatttcctag agaagatttg
     6781 gacatgatac tgaaaatgga ttctttgcaa gacataaaag cccttctgac ggggctgttt
     6841 gtccaggata atgtcaacaa ggatgcagta actgatcgct tggcttccgt agagacagac
     6901 atgccattga cactgcggca acaccgaatt tctgctactt ccagctcaga ggaatcatcc
     6961 aataagggtc aaaggcagct gaccgtttcg ggaggaggtg gaagcggagg aggaggaagc
     7021 ggaggaggag gtagcggacc taagaaaaag aggaaggtgg cggccgctgg atccgatgct
     7081 ttagacgatt ttgacttaga tatgcttggt tcagacgcgt tagacgactt cgacctagac
     7141 atgttaggct cagatgcatt ggacgacttc gatttagata tgttgggctc cgatgcccta
     7201 gatgactttg atctagatat gctaggtagt ggcaagccga tccctaaccc tctgctgggc
     7261 ctggacagca cttaggaatt cgatatcatc ggtaatcaac ctctggatta caaaatttgt
     7321 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
     7381 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
     7441 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
     7501 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
     7561 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
     7621 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
     7681 tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc
     7741 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
     7801 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
     7861 tccctttggg ccgcctcccc gcgtcgactt taagaccaat gacttacaag gcagctgtag
     7921 atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa
     7981 gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg
     8041 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
     8101 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
     8161 tttagtcagt gtggaaaatc tctagcagta cgtatagtag ttcatgtcat cttattattc
     8221 agtatttata acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag
     8281 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     8341 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct
     8401 agctatcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     8461 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
     8521 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctagggacgt acccaattcg
     8581 ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg
     8641 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
     8701 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
     8761 gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     8821 gtgaccgcta cacttgccag cgccatagcg cccgctcctt tcgctttctt cccttccttt
     8881 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     8941 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
     9001 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     9061 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
     9121 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
     9181 aaatttaacg cgaattttaa caaaatatta acgcttacaa tttaggtggc acttttcggg
     9241 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     9301 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     9361 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     9421 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     9481 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     9541 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     9601 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     9661 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     9721 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     9781 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     9841 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     9901 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     9961 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
    10021 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
    10081 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
    10141 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
    10201 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
    10261 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
    10321 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
    10381 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
    10441 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
    10501 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
    10561 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
    10621 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
    10681 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagccca
//
