LOCUS       sequence-335667-        8596 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  HiLITR protease with MAVS [R537A] C-terminal tmd targeting 
            information (Mitochondria and ER insertion).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8596)
  AUTHORS   Coukos RW, Yao D, Sanchez ML, Strand ET, Olive ME, Udeshi ND, 
            Weissman JS, Carr SA, Bassik MC, Ting AY
  TITLE     An engineered transcriptional reporter of protein localization 
            identifies regulators of mitochondrial and ER membrane protein 
            trafficking in high-throughput CRISPRi screens.
  JOURNAL   eLife. 2021 Aug 20;10. pii: 69142. doi: 10.7554/eLife.69142.
  PUBMED    34414886
REFERENCE   2  (bases 1 to 8596)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife."; 
            date: "2021-08-20"; volume: "10. pii: 69142. doi"; pages: " 
            10.7554/eLife.69142"
COMMENT     Sequence Label: EGFP-uTEV1-MAVS-mTA
FEATURES             Location/Qualifiers
     source          1..8596
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        51..365
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    59..77
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    95..113
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    130..148
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    166..184
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    202..220
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    237..255
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    273..291
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     338..362
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      382..391
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(388..390,391..393,394..1104)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(388..390,391..393,394..1104)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(433..454)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(694..713)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1041..1062
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        2028..2538
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     2457..2475
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             join(2548..3165,3166..3171,3172..3207,3208..3210,3211..3246,
                     3247..3249,3250..3285,3286..3294)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(3334..3354)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3752..3763)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3968..4201
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4273..4394
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4310..4329)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4364..4383
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4434..4569
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        4569..4640
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4641..4709,4710..5501)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4859..4878)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5672..6260
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6161..6180
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6414..6431
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6548..6569
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6584..6589,6590..6607,6608..6614)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6622..6638
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6627..6649
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6646..6662
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6646..6662
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6681..6701
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6683..6701
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6729..6955
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6956..7136
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7183..7308
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7801..8034
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8219..8263
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8476..8593
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 gtttattaca gggacagcag agatccactt tggccgcgaa tcgatatgtc gagtttactc
       61 cctatcagtg atagagaacg tatgtcgagt ttactcccta tcagtgatag agaacgatgt
      121 cgagtttact ccctatcagt gatagagaac gtatgtcgag tttactccct atcagtgata
      181 gagaacgtat gtcgagttta ctccctatca gtgatagaga acgtatgtcg agtttatccc
      241 tatcagtgat agagaacgta tgtcgagttt actccctatc agtgatagag aacgtatgtc
      301 gaggtaggcg tgtacggtgg gaggcctata taagcagagc tcgtttagtg aaccgtcaga
      361 tcgcctggag aattggctag cgccaccatg gtgagcaagg gcgaggagct gttcaccggg
      421 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc
      481 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc
      541 ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc
      601 ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
      661 ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc
      721 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
      781 aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc
      841 tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac
      901 atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac
      961 ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct gagcaaagac
     1021 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact
     1081 ctcggcatgg acgagctgta caaggacctt ggatctggca caggcggatc cggttcaggc
     1141 ggagaaagct tgtttaaggg accacgtgat tacaacccga tatcgagcac catttgtcat
     1201 ttgacgaatg aatctgatgg gcacacaaca tcgttgtatg gtattggatt tggtcccttc
     1261 atcattacaa acaagcactt gtttagaaga aataatggaa cactgttggt ccaatcacta
     1321 catggtgtat tcaaggtcaa gaacaccacg actttgcaac aacacctcat tgatgggagg
     1381 gacatgataa ttattcgcat gcctaaggat ttcccaccat ttcctcaaaa gctgaaattt
     1441 agagagccac aaagggaaga gcgcatatgt cttgtgacaa ccaacttcca aactaagagc
     1501 atgtctagca tggtgtcaga cactagttgc acattccctt catctgatgg catattctgg
     1561 aagcattgga ttcaaaccaa ggatgggcag tgtggcaatc cattagtatc aactagagat
     1621 gggttcattg ttggtataca ctcagcatcg aatttcacca acacaaacaa ttatttcaca
     1681 agcgtgccga aaaacttcat ggaattgttg acaaatcagg aggcgcagca gtgggttagt
     1741 ggttggcgat taaatgctga ctcagtattg tgggggggcc ataaagtttt catggtggaa
     1801 ttcggttcca cgtcaggttc tggcaagccc ggatcaggcg agggtagcgg ctctacttct
     1861 gggtctggta agcctgggag tggagagggg agcggcagtt ccggctcagg tagtggaagg
     1921 ccctcacctg gggctctgtg gctccaggtg gctgtgacag gggtgctggt agtcacactc
     1981 ctggtggtgc tgtaccgggc gcgtctgcac tagggccgaa ttcatccggg gttggggttg
     2041 cgccttttcc aaggcagccc tgggtttgcg cagggacgcg gctgctctgg gcgtggttcc
     2101 gggaaacgca gcggcgccga ccctgggtct cgcacattct tcacgtccgt tcgcagcgtc
     2161 acccggatct tcgccgctac ccttgtgggc cccccggcga cgcttcctgc tccgccccta
     2221 agtcgggaag gttccttgcg gttcgcggcg tgccggacgt gacaaacgga agccgcacgt
     2281 ctcactagta ccctcgcaga cggacagcgc cagggagcaa tggcagcgcg ccgaccgcga
     2341 tgggctgtgg ccaatagcgg ctgctcagca gggcgcgccg agagcagcgg ccgggaaggg
     2401 gcggtgcggg aggcggggtg tggggcggta gtgtgggccc tgttcctgcc cgcgcggtgt
     2461 tccgcattct gcaagcctcc ggagcgcacg tcggcagtcg gctccctcgt tgaccgaatc
     2521 accgacctct ctccccagca attcaccatg tctagactgg acaagagcaa agtcataaac
     2581 ggagctctgg aattactcaa tggtgtcggt atcgaaggcc tgacgacaag gaaactcgct
     2641 caaaagctgg gagttgagca gcctaccctg tactggcacg tgaagaacaa gcgggccctg
     2701 ctcgatgccc tgccaatcga gatgctggac aggcatcata cccacttctg ccccctggaa
     2761 ggcgagtcat ggcaagactt tctgcggaac aacgccaagt cataccgctg tgctctcctc
     2821 tcacatcgcg acggggctaa agtgcatctc ggcacccgcc caacagagaa acagtacgaa
     2881 accctggaaa atcagctcgc gttcctgtgt cagcaaggct tctccctgga gaacgcactg
     2941 tacgctctgt ccgccgtggg ccactttaca ctgggctgcg tattggagga acaggagcat
     3001 caagtagcaa aagaggaaag agagacacct accaccgatt ctatgccccc acttctgaga
     3061 caagcaattg agctgttcga ccggcaggga gccgaacctg ccttcctttt cggcctggaa
     3121 ctaatcatat gtggcctgga gaaacagcta aagtgcgaaa gcggcgggcc gaccgacgcc
     3181 cttgacgatt ttgacttaga catgctccca gccgatgccc ttgacgactt tgaccttgat
     3241 atgctgcctg ctgacgctct tgacgatttt gaccttgaca tgctccccgg gtaactaagt
     3301 aaggatcgat ccaagatatc gtattcttaa ctatgttgct ccttttacgc tatgtggata
     3361 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc
     3421 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg
     3481 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac
     3541 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat
     3601 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt
     3661 ggtgttgtcg gggaagctga cgtcctttcc atggctgctc gcctgtgttg ccacctggat
     3721 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc
     3781 ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag
     3841 tcggatctcc ctttgggccg cctccccgcc tgtttcgcct cggcgtccgg actagaggta
     3901 cctttaagac caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag
     3961 gggggactgg aagggctaat tcactcccaa cgaagacaag atctgctttt tgcttgtact
     4021 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
     4081 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
     4141 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
     4201 agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca
     4261 gagagtgaga ggaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     4321 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     4381 atcaatgtat cttatcatgt ctggctctag ctatcccgcc cctaactccg cccatcccgc
     4441 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
     4501 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt
     4561 ttggaggcct ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     4621 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     4681 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     4741 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     4801 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     4861 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     4921 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     4981 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     5041 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     5101 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     5161 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     5221 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     5281 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     5341 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     5401 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     5461 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     5521 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     5581 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     5641 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     5701 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     5761 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     5821 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     5881 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     5941 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     6001 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     6061 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     6121 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     6181 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     6241 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     6301 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     6361 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     6421 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     6481 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     6541 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     6601 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
     6661 accatgatta cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctgc
     6721 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta
     6781 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg
     6841 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac
     6901 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc
     6961 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     7021 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     7081 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg
     7141 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact
     7201 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa
     7261 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg
     7321 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat
     7381 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc
     7441 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag
     7501 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat
     7561 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac
     7621 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat
     7681 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg
     7741 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat
     7801 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat
     7861 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt
     7921 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca
     7981 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat
     8041 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag
     8101 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat
     8161 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa
     8221 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat
     8281 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt
     8341 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt
     8401 atcgtttcag acccacctcc caaccccgag gggacaattc tcgacctcga gacaaatggc
     8461 agtattcatc cacaatttta aaagaaaagg ggggattggg gggtacagtg caggggaaag
     8521 aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa
     8581 aattcaaaat tttcgg
//
