LOCUS       sequence-335741-       10478 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway destination clone of CSNK1A1L (human) tagged with N-terminal
            miniTurbo-V5 for generating protein-proximity networks using 
            proximity-dependent biotinylation proteomics.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10478)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 10478)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p663-UBC-miniTurbo-V5-CSNK1A1L_IDG-K
FEATURES             Location/Qualifiers
     source          1..10478
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    115..231
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    265..285
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        285..1496
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     1333..1352
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    complement(1500..1520)
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     regulatory      1521..1530
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1533..2303
                     /codon_start=1
                     /product="N-terminally truncated mutant of the E. coli 
                     biotin ligase BioID engineered for improved catalytic 
                     efficiency (Branon et al., 2018)"
                     /label=miniTurbo
                     /translation="IPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGDACI
                     AEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLGADK
                     VRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWITLQ
                     EAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIF
                     GISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     CDS             2310..2351
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    2352..2376
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3397..3421)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    3451..4039
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3504..3524)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3922..3933)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        4053..4552
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4088..4111)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4485..4504
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             4573..5172
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4573..4592)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5069..5089
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             5310..5543
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5615..5736
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5652..5671)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5706..5725
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5776..5911
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5931..5950)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5932..5950)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5960..5977)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5960..5976)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5969..5991)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6118..6573
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6205..6224)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6415..6436
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6599..6703
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6704..6772,6773..7564)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      7735..8323
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8224..8243
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8477..8494
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8611..8632
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8647..8652,8653..8670,8671..8677)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8685..8701
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8690..8712
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8709..8725
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8709..8725
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8744..8764
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8746..8764
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8792..9018
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             9019..9199
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9246..9371
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9864..10097
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10282..10326
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 cctcccaacc ccgaggggac ccgacaggcc cgaaggaata gaagaagaag gtggagagag
       61 agacagagac agatccattc gattagtgaa cggatctcga cggtatcggt taacttttaa
      121 aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac
      181 agacatacaa actaaagaat tacaaaaaca aattacaaaa ttcaaaattt tatcgtccag
      241 tgtggtggaa ttctgcagat atcacaactt tgtatagaaa agttggcctc cgcgccgggt
      301 tttggcgcct cccgcgggcg cccccctcct cacggcgagc gctgccacgt cagacgaagg
      361 gcgcagcgag cgtcctgatc cttccgcccg gacgctcagg acagcggccc gctgctcata
      421 agactcggcc ttagaacccc agtatcagca gaaggacatt ttaggacggg acttgggtga
      481 ctctagggca ctggttttct ttccagagag cggaacaggc gaggaaaagt agtcccttct
      541 cggcgattct gcggagggat ctccgtgggg cggtgaacgc cgatgattat ataaggacgc
      601 gccgggtgtg gcacagctag ttccgtcgca gccgggattt gggtcgcggt tcttgtttgt
      661 ggatcgctgt gatcgtcact tggtgagtag cgggctgctg ggctggccgg ggctttcgtg
      721 gccgccgggc cgctcggtgg gacggaagcg tgtggagaga ccgccaaggg ctgtagtctg
      781 ggtccgcgag caaggttgcc ctgaactggg ggttgggggg agcgcagcaa aatggcggct
      841 gttcccgagt cttgaatgga agacgcttgt gaggcgggct gtgaggtcgt tgaaacaagg
      901 tggggggcat ggtgggcggc aagaacccaa ggtcttgagg ccttcgctaa tgcgggaaag
      961 ctcttattcg ggtgagatgg gctggggcac catctgggga ccctgacgtg aagtttgtca
     1021 ctgactggag aactcggttt gtcgtctgtt gcgggggcgg cagttatggc ggtgccgttg
     1081 ggcagtgcac ccgtaccttt gggagcgcgc gccctcgtcg tgtcgtgacg tcacccgttc
     1141 tgttggctta taatgcaggg tggggccacc tgccggtagg tgtgcggtag gcttttctcc
     1201 gtcgcaggac gcagggttcg ggcctagggt aggctctcct gaatcgacag gcgccggacc
     1261 tctggtgagg ggagggataa gtgaggcgtc agtttctttg gtcggtttta tgtacctatc
     1321 ttcttaagta gctgaagctc cggttttgaa ctatgcgctc ggggttggcg agtgtgtttt
     1381 gtgaagtttt ttaggcacct tttgaaatgt aatcatttgg gtcaatatgt aattttcagt
     1441 gttagactag taaattgtcc gctaaattct ggccgttttt ggcttttttg ttagacggcc
     1501 aacttttgta tacaaagtgg gccaccatgg gaatcccgct gctgaacgct aaacagattc
     1561 tgggacagct ggacggcggg agcgtggcag tcctgcctgt ggtcgactcc accaatcagt
     1621 acctgctgga tcgaatcggc gagctgaaga gtggggatgc ttgcattgca gaatatcagc
     1681 aggcagggag aggaagcaga gggaggaaat ggttctctcc ttttggagct aacctgtacc
     1741 tgagtatgtt ttggcgcctg aagcggggac cagcagcaat cggcctgggc ccggtcatcg
     1801 gaattgtcat ggcagaagcg ctgcgaaagc tgggagcaga caaggtgcga gtcaaatggc
     1861 ccaatgacct gtatctgcag gatagaaagc tggcaggcat cctggtggag ctggccggaa
     1921 taacaggcga tgctgcacag atcgtcattg gcgccgggat taacgtggct atgaggcgcg
     1981 tggaggaaag cgtggtcaat cagggctgga tcacactgca ggaagcaggg attaacctgg
     2041 acaggaatac tctggccgct atgctgatcc gagagctgcg ggcagccctg gaactgttcg
     2101 agcaggaagg cctggctcca tatctgtcac ggtgggagaa gctggataac ttcatcaata
     2161 gacccgtgaa gctgatcatt ggggacaaag agattttcgg gattagccgg gggattgata
     2221 aacagggagc cctgctgctg gaacaggacg gagttatcaa accctggatg ggcggagaaa
     2281 tcagtctgcg gtctgccgaa aaggctagcg gcaagcccat ccccaacccc ctgctgggcc
     2341 tggacagcac cacaagtttg tacaaaaaag caggctccac catgacaaac aacagcggct
     2401 ccaaagccga actcgttgtg ggagggaaat acaaactggt gcggaagatc gggtctggct
     2461 cctttggaga cgtttatctg ggcatcacca ccaccaacgg cgaggaagta gcagtgaagc
     2521 tggaatctca gaaggtcaag cacccccagt tgctgtatga gagcaaactc tacacgattc
     2581 ttcaaggtgg ggttggcatc ccccacatgc actggtatgg tcaggaaaaa gacaacaatg
     2641 tgctagtcat ggaccttctg ggacccagcc tcgaagacct ctttaatttc tgttcaagaa
     2701 ggttcaccat gaaaactgta cttatgttag ccgaccagat gatcagcaga attgaatacg
     2761 tgcatacaaa gaattttcta caccgagaca ttaaaccaga taacttcctg atgggtactg
     2821 ggcgtcactg taataagttg ttccttattg attttggttt ggccaaaaag tacagagaca
     2881 acaggaccag gcaacacata ccgtacagag aagataaaca cctcattggc actgtccgat
     2941 atgccagcat caatgcacat cttggtattg agcagagccg ccgagatgac atggaatcct
     3001 taggctacgt tttcatgtat tttaatagaa ccagcctgcc gtggcaagga ctaaaggcta
     3061 tgacaaaaaa acaaaaatat gaaaagatta gtgagaagaa gatgtccacc cctgttgaag
     3121 ttttatgtaa ggggtttcct gcagaattcg ccatgtactt gaactactgt cgtgggctgc
     3181 gctttgagga agtcccagat tacatgtatc tgaggcagct attccgcatt cttttcagga
     3241 ccctgaacca ccaatatgac tacacatttg attggacgat gttaaagcag aaagcagcac
     3301 agcaggcagc ctcttccagt gggcagggtc agcaggccca aacccagaca ggcaagcaaa
     3361 ctgaaaaaaa caagaataat gtgaaagata actaggaccc agctttcttg tacaaagtgg
     3421 tgatatccag cacagtggcg gccgctcgac aatcaacctc tggattacaa aatttgtgaa
     3481 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
     3541 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
     3601 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
     3661 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
     3721 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
     3781 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
     3841 gggaagctga cgtcctttcc atggctgctc gcctgtgttg ccacctggat tctgcgcggg
     3901 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
     3961 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc
     4021 ctttgggccg cctccccgcc tggaattcta ccgggtaggg gaggcgcttt tcccaaggca
     4081 gtctggagca tgcgctttag cagccccgct gggcacttgg cgctacacaa gtggcctctg
     4141 gcctcgcaca cattccacat ccaccggtag gcgccaaccg gctccgttct ttggtggccc
     4201 cttcgcgcca ccttctactc ctcccctagt caggaagttc ccccccgccc cgcagctcgc
     4261 gtcgtgcagg acgtgacaaa tggaagtagc acgtctcact agtctcgtgc agatggacag
     4321 caccgctgag caatggaagc gggtaggcct ttggggcagc ggccaatagc agctttgctc
     4381 cttcgctttc tgggctcaga ggctgggaag gggtgggtcc gggggcgggc tcaggggcgg
     4441 gctcaggggc ggggcgggcg cccgaaggtc ctccggaggc ccggcattct gcacgcttca
     4501 aaagcgcacg tctgccgcgc tgttctcctc ttcctcatct ccgggccttt cgacctgcag
     4561 cccaagctta ccatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc
     4621 cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc
     4681 gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc
     4741 gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg
     4801 accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc
     4861 gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac
     4921 cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc
     4981 aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg
     5041 cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc
     5101 accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag
     5161 cccggtgcct gacgcccgcc ccacgacccg cagcgcccga ccgaaaggag cgcacgaccc
     5221 catgcatctc gagggcccgg tacctttaag accaatgact tacaaggcag ctgtagatct
     5281 tagccacttt ttaaaagaaa aggggggact ggaagggcta gctcactccc aacgaagaca
     5341 agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct
     5401 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
     5461 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
     5521 gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata
     5581 acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg
     5641 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     5701 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctatcccg
     5761 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     5821 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
     5881 cagaagtagt gaggaggctt ttttggaggc ctagggacgt acccaattcg ccctatagtg
     5941 agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg
     6001 gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg
     6061 aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgggacg
     6121 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
     6181 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
     6241 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
     6301 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat
     6361 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
     6421 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag
     6481 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
     6541 cgaattttaa caaaatatta acgcttacaa tttaggtggc acttttcggg gaaatgtgcg
     6601 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     6661 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     6721 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     6781 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     6841 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     6901 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
     6961 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     7021 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     7081 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     7141 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     7201 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     7261 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     7321 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     7381 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     7441 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     7501 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     7561 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     7621 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     7681 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     7741 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     7801 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     7861 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
     7921 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     7981 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     8041 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     8101 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     8161 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     8221 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     8281 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     8341 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     8401 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
     8461 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa
     8521 accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga
     8581 ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc
     8641 ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca
     8701 atttcacaca ggaaacagct atgaccatga ttacgccaag cgcgcaatta accctcacta
     8761 aagggaacaa aagctggagc tgcaagctta atgtagtctt atgcaatact cttgtagtct
     8821 tgcaacatgg taacgatgag ttagcaacat gccttacaag gagagaaaaa gcaccgtgca
     8881 tgccgattgg tggaagtaag gtggtacgat cgtgccttat taggaaggca acagacgggt
     8941 ctgacatgga ttggacgaac cactgaattg ccgcattgca gagatattgt atttaagtgc
     9001 ctagctcgat acataaacgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
     9061 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
     9121 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
     9181 gtgtggaaaa tctctagcag tggcgcccga acagggactt gaaagcgaaa gggaaaccag
     9241 aggagctctc tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc
     9301 ggcgactggt gagtacgcca aaaattttga ctagcggagg ctagaaggag agagatgggt
     9361 gcgagagcgt cagtattaag cgggggagaa ttagatcgcg atgggaaaaa attcggttaa
     9421 ggccaggggg aaagaaaaaa tataaattaa aacatatagt atgggcaagc agggagctag
     9481 aacgattcgc agttaatcct ggcctgttag aaacatcaga aggctgtaga caaatactgg
     9541 gacagctaca accatccctt cagacaggat cagaagaact tagatcatta tataatacag
     9601 tagcaaccct ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag
     9661 acaagataga ggaagagcaa aacaaaagta agaccaccgc acagcaagcg gccgctgatc
     9721 ttcagacctg gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa
     9781 gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag
     9841 agagaaaaaa gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga
     9901 agcactatgg gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt
     9961 atagtgcagc agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa
    10021 ctcacagtct ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta
    10081 aaggatcaac agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct
    10141 gtgccttgga atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc
    10201 tggatggagt gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa
    10261 gaatcgcaaa accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca
    10321 agtttgtgga attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg
    10381 atagtaggag gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga
    10441 gttaggcagg gatattcacc attatcgttt cagaccca
//
