LOCUS       sequence-335744-       11642 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway destination clone of EEF2K (human) tagged with N-terminal 
            miniTurbo-V5 for generating protein-proximity networks using 
            proximity-dependent biotinylation proteomics.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11642)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 11642)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p663-UBC-miniTurbo-V5-EEF2K_IDG-K
FEATURES             Location/Qualifiers
     source          1..11642
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    177..293
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    327..347
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        347..1558
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     1395..1414
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    complement(1562..1582)
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     regulatory      1583..1592
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1595..2365
                     /codon_start=1
                     /product="N-terminally truncated mutant of the E. coli 
                     biotin ligase BioID engineered for improved catalytic 
                     efficiency (Branon et al., 2018)"
                     /label=miniTurbo
                     /translation="IPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGDACI
                     AEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLGADK
                     VRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWITLQ
                     EAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIF
                     GISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     CDS             2372..2413
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    2414..2438
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4623..4647)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    4677..5265
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4730..4750)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5148..5159)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        5279..5778
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5314..5337)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5711..5730
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             5799..6398
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5799..5818)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6295..6315
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             6536..6769
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6841..6962
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6878..6897)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6932..6951
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7002..7137
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7157..7176)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7158..7176)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7186..7203)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7186..7202)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7195..7217)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7344..7799
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7431..7450)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7641..7662
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7825..7929
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7930..7998,7999..8790)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      8961..9549
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9450..9469
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9703..9720
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9837..9858
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9873..9878,9879..9896,9897..9903)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9911..9927
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9916..9938
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9935..9951
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9935..9951
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9970..9990
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9972..9990
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        10018..10244
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             10245..10425
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    10472..10597
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11090..11323
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11508..11552
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc gtttcagacc
       61 cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga aggtggagag
      121 agagacagag acagatccat tcgattagtg aacggatctc gacggtatcg gttaactttt
      181 aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca
      241 acagacatac aaactaaaga attacaaaaa caaattacaa aattcaaaat tttatcgtcc
      301 agtgtggtgg aattctgcag atatcacaac tttgtataga aaagttggcc tccgcgccgg
      361 gttttggcgc ctcccgcggg cgcccccctc ctcacggcga gcgctgccac gtcagacgaa
      421 gggcgcagcg agcgtcctga tccttccgcc cggacgctca ggacagcggc ccgctgctca
      481 taagactcgg ccttagaacc ccagtatcag cagaaggaca ttttaggacg ggacttgggt
      541 gactctaggg cactggtttt ctttccagag agcggaacag gcgaggaaaa gtagtccctt
      601 ctcggcgatt ctgcggaggg atctccgtgg ggcggtgaac gccgatgatt atataaggac
      661 gcgccgggtg tggcacagct agttccgtcg cagccgggat ttgggtcgcg gttcttgttt
      721 gtggatcgct gtgatcgtca cttggtgagt agcgggctgc tgggctggcc ggggctttcg
      781 tggccgccgg gccgctcggt gggacggaag cgtgtggaga gaccgccaag ggctgtagtc
      841 tgggtccgcg agcaaggttg ccctgaactg ggggttgggg ggagcgcagc aaaatggcgg
      901 ctgttcccga gtcttgaatg gaagacgctt gtgaggcggg ctgtgaggtc gttgaaacaa
      961 ggtggggggc atggtgggcg gcaagaaccc aaggtcttga ggccttcgct aatgcgggaa
     1021 agctcttatt cgggtgagat gggctggggc accatctggg gaccctgacg tgaagtttgt
     1081 cactgactgg agaactcggt ttgtcgtctg ttgcgggggc ggcagttatg gcggtgccgt
     1141 tgggcagtgc acccgtacct ttgggagcgc gcgccctcgt cgtgtcgtga cgtcacccgt
     1201 tctgttggct tataatgcag ggtggggcca cctgccggta ggtgtgcggt aggcttttct
     1261 ccgtcgcagg acgcagggtt cgggcctagg gtaggctctc ctgaatcgac aggcgccgga
     1321 cctctggtga ggggagggat aagtgaggcg tcagtttctt tggtcggttt tatgtaccta
     1381 tcttcttaag tagctgaagc tccggttttg aactatgcgc tcggggttgg cgagtgtgtt
     1441 ttgtgaagtt ttttaggcac cttttgaaat gtaatcattt gggtcaatat gtaattttca
     1501 gtgttagact agtaaattgt ccgctaaatt ctggccgttt ttggcttttt tgttagacgg
     1561 ccaacttttg tatacaaagt gggccaccat gggaatcccg ctgctgaacg ctaaacagat
     1621 tctgggacag ctggacggcg ggagcgtggc agtcctgcct gtggtcgact ccaccaatca
     1681 gtacctgctg gatcgaatcg gcgagctgaa gagtggggat gcttgcattg cagaatatca
     1741 gcaggcaggg agaggaagca gagggaggaa atggttctct ccttttggag ctaacctgta
     1801 cctgagtatg ttttggcgcc tgaagcgggg accagcagca atcggcctgg gcccggtcat
     1861 cggaattgtc atggcagaag cgctgcgaaa gctgggagca gacaaggtgc gagtcaaatg
     1921 gcccaatgac ctgtatctgc aggatagaaa gctggcaggc atcctggtgg agctggccgg
     1981 aataacaggc gatgctgcac agatcgtcat tggcgccggg attaacgtgg ctatgaggcg
     2041 cgtggaggaa agcgtggtca atcagggctg gatcacactg caggaagcag ggattaacct
     2101 ggacaggaat actctggccg ctatgctgat ccgagagctg cgggcagccc tggaactgtt
     2161 cgagcaggaa ggcctggctc catatctgtc acggtgggag aagctggata acttcatcaa
     2221 tagacccgtg aagctgatca ttggggacaa agagattttc gggattagcc gggggattga
     2281 taaacaggga gccctgctgc tggaacagga cggagttatc aaaccctgga tgggcggaga
     2341 aatcagtctg cggtctgccg aaaaggctag cggcaagccc atccccaacc ccctgctggg
     2401 cctggacagc accacaagtt tgtacaaaaa agcaggctcc accatggcag acgaagatct
     2461 catcttccgc ctggaaggcg ttgatggcgg ccagtccccc cgagctggcc gtgatggtga
     2521 ttctgatggg gacagcgacg atgaggaagg ttacttcatc tgccccatca cggatgaccc
     2581 aagctcgaac cagaatgtca attccaaggt taataagtac tacagcaacc taacaaaaag
     2641 tgagcggtat agctccagcg ggtccccggc aaactccttc cacttcaagg aagcctggaa
     2701 gcacgcaatc cagaaggcca agcacatgcc cgacccctgg gctgagttcc acctggaaga
     2761 tattgccacc gaacgtgcta ctcgacacag gtacaacgcc gtcaccgggg aatggctgga
     2821 tgatgaagtt ctgatcaaga tggcatctca gcccttcggc cgaggagcaa tgagggagtg
     2881 cttccggacg aagaagctct ccaacttctt gcatgcccag cagtggaagg gagcctccaa
     2941 ctacgtggcg aagcgctaca tcgagcccgt agaccgggat gtgtactttg aggacgtgcg
     3001 tctacagatg gaggccaagc tctgggggga ggagtataat cggcacaagc cccccaagca
     3061 ggtggacatc atgcagatgt gcatcatcga gctgaaggac agaccgggca agcccctctt
     3121 ccacctggag cactacatcg agggcaagta catcaagtac aactccaact ctggctttgt
     3181 ccgcgatgac aacatccgcc tgacgccgca ggccttcagc cacttcactt ttgagcgttc
     3241 cggccatcag ctgatagtgg tggacatcca gggagttggg gatctctaca ctgacccaca
     3301 gatccacacg gagacgggca ctgactttgg agacggcaac ctaggtgtcc gcgggatggc
     3361 gctcttcttc tactctcatg cctgcaaccg gatttgcgag agcatgggcc ttgctccctt
     3421 tgacctctcg ccccgggaga gggatgcagt gaatcagaac accaagctgc tgcaatcagc
     3481 caagaccatc ttgagaggaa cagaggaaaa atgtgggagc ccccgagtaa ggaccctctc
     3541 tgggagccgg ccacccctgc tccgtcccct ttcagagaac tctggagacg agaacatgag
     3601 cgacgtgacc ttcgactctc tcccttcttc cccatcttcg gccacaccac acagccagaa
     3661 gctagaccac ctccattggc cagtgttcag tgacctcgat aacatggcat ccagagacca
     3721 tgatcatcta gacaaccacc gggagtctga gaatagtggg gacagcggat accccagtga
     3781 gaagcggggt gagctggatg accctgagcc ccgagaacat ggccactcat acagtaatcg
     3841 gaagtacgag tctgacgaag acagcctggg cagctctgga cgggtatgtg tagagaagtg
     3901 gaatctcctc aactcctccc gcctccacct gccgagggct tcggccgtgg ccctggaagt
     3961 gcaaaggctt aatgctctgg acctcgaaaa gaaaatcggg aagtccattt tggggaaggt
     4021 ccatctggcc atggtgcgct accacgaggg tgggcgcttc tgcgagaagg gcgaggagtg
     4081 ggaccaggag tcggctgtct tccacctgga gcacgcagcc aacctgggcg agctggaggc
     4141 catcgtgggc ctgggactca tgtactcgca gttgcctcat cacatcctag ccgatgtctc
     4201 tctgaaggag acagaagaga acaaaaccaa aggatttgat tacttactaa aggccgctga
     4261 agctggcgac aggcagtcca tgatcctagt ggcgcgagct tttgactctg gccagaacct
     4321 cagcccggac aggtgccaag actggctaga ggccctgcac tggtacaaca ctgccctgga
     4381 gatgacggac tgtgatgagg gcggtgagta cgacggaatg caggacgagc cccggtacat
     4441 gatgctggcc agggaggccg agatgctgtt cacaggaggc tacgggctgg agaaggaccc
     4501 gcagagatca ggggacttgt atacccaggc agcagaggca gcgatggaag ccatgaaggg
     4561 ccgactggcc aaccagtact accaaaaggc tgaagaggcc tgggcccaga tggaggagta
     4621 ggacccagct ttcttgtaca aagtggtgat atccagcaca gtggcggccg ctcgacaatc
     4681 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt
     4741 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg
     4801 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc
     4861 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt
     4921 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg
     4981 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg
     5041 gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct
     5101 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc
     5161 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc
     5221 ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcctgga attctaccgg
     5281 gtaggggagg cgcttttccc aaggcagtct ggagcatgcg ctttagcagc cccgctgggc
     5341 acttggcgct acacaagtgg cctctggcct cgcacacatt ccacatccac cggtaggcgc
     5401 caaccggctc cgttctttgg tggccccttc gcgccacctt ctactcctcc cctagtcagg
     5461 aagttccccc ccgccccgca gctcgcgtcg tgcaggacgt gacaaatgga agtagcacgt
     5521 ctcactagtc tcgtgcagat ggacagcacc gctgagcaat ggaagcgggt aggcctttgg
     5581 ggcagcggcc aatagcagct ttgctccttc gctttctggg ctcagaggct gggaaggggt
     5641 gggtccgggg gcgggctcag gggcgggctc aggggcgggg cgggcgcccg aaggtcctcc
     5701 ggaggcccgg cattctgcac gcttcaaaag cgcacgtctg ccgcgctgtt ctcctcttcc
     5761 tcatctccgg gcctttcgac ctgcagccca agcttaccat gaccgagtac aagcccacgg
     5821 tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc gccgcgttcg
     5881 ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag cgggtcaccg
     5941 agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg
     6001 acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt
     6061 tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac
     6121 agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg
     6181 tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag
     6241 tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc
     6301 tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac
     6361 cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgacg cccgccccac gacccgcagc
     6421 gcccgaccga aaggagcgca cgaccccatg catctcgagg gcccggtacc tttaagacca
     6481 atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa
     6541 gggctagctc actcccaacg aagacaagat ctgctttttg cttgtactgg gtctctctgg
     6601 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
     6661 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
     6721 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca
     6781 tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg
     6841 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     6901 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     6961 tatcatgtct ggctctagct atcccgcccc taactccgcc catcccgccc ctaactccgc
     7021 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     7081 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     7141 ggacgtaccc aattcgccct atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt
     7201 acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc
     7261 ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt
     7321 gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
     7381 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
     7441 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
     7501 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta
     7561 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
     7621 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
     7681 ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa
     7741 tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaattta
     7801 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     7861 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     7921 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
     7981 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
     8041 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
     8101 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
     8161 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
     8221 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
     8281 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
     8341 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
     8401 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     8461 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
     8521 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
     8581 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     8641 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
     8701 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     8761 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
     8821 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     8881 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     8941 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     9001 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     9061 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     9121 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     9181 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     9241 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     9301 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     9361 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     9421 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     9481 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     9541 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     9601 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     9661 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     9721 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
     9781 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat
     9841 gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg
     9901 ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac
     9961 gccaagcgcg caattaaccc tcactaaagg gaacaaaagc tggagctgca agcttaatgt
    10021 agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag caacatgcct
    10081 tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg
    10141 ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact gaattgccgc
    10201 attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct ctctggttag
    10261 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
    10321 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
    10381 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag
    10441 ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga
    10501 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag
    10561 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag
    10621 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca
    10681 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac
    10741 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga
    10801 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga
    10861 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac
    10921 caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt
    10981 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca
    11041 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt
    11101 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg
    11161 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta
    11221 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa
    11281 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct
    11341 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc
    11401 tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca
    11461 caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag
    11521 aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc
    11581 tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt
    11641 tt
//
