LOCUS       sequence-335745-       10862 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway destination clone of MKNK1 (human) tagged with N-terminal 
            miniTurbo-V5 for generating protein-proximity networks using 
            proximity-dependent biotinylation proteomics.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10862)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 10862)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p663-UBC-miniTurbo-V5-MKNK1_IDG-K
FEATURES             Location/Qualifiers
     source          1..10862
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    68..184
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    218..238
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        238..1449
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     1286..1305
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    complement(1453..1473)
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     regulatory      1474..1483
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1486..2256
                     /codon_start=1
                     /product="N-terminally truncated mutant of the E. coli 
                     biotin ligase BioID engineered for improved catalytic 
                     efficiency (Branon et al., 2018)"
                     /label=miniTurbo
                     /translation="IPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGDACI
                     AEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLGADK
                     VRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWITLQ
                     EAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIF
                     GISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     CDS             2263..2304
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    2305..2329
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3734..3758)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    3788..4376
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3841..3861)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4259..4270)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        4390..4889
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4425..4448)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4822..4841
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             4910..5509
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4910..4929)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5406..5426
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             5647..5880
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5952..6073
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5989..6008)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6043..6062
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6113..6248
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6268..6287)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6269..6287)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6297..6314)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6297..6313)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6306..6328)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6455..6910
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6542..6561)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6752..6773
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6936..7040
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7041..7109,7110..7901)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      8072..8660
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8561..8580
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8814..8831
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8948..8969
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8984..8989,8990..9007,9008..9014)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9022..9038
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9027..9049
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9046..9062
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9046..9062
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9081..9101
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9083..9101
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        9129..9355
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             9356..9536
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9583..9708
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10201..10434
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10619..10663
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatct cgacggtatc
       61 ggttaacttt taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag
      121 acataatagc aacagacata caaactaaag aattacaaaa acaaattaca aaattcaaaa
      181 ttttatcgtc cagtgtggtg gaattctgca gatatcacaa ctttgtatag aaaagttggc
      241 ctccgcgccg ggttttggcg cctcccgcgg gcgcccccct cctcacggcg agcgctgcca
      301 cgtcagacga agggcgcagc gagcgtcctg atccttccgc ccggacgctc aggacagcgg
      361 cccgctgctc ataagactcg gccttagaac cccagtatca gcagaaggac attttaggac
      421 gggacttggg tgactctagg gcactggttt tctttccaga gagcggaaca ggcgaggaaa
      481 agtagtccct tctcggcgat tctgcggagg gatctccgtg gggcggtgaa cgccgatgat
      541 tatataagga cgcgccgggt gtggcacagc tagttccgtc gcagccggga tttgggtcgc
      601 ggttcttgtt tgtggatcgc tgtgatcgtc acttggtgag tagcgggctg ctgggctggc
      661 cggggctttc gtggccgccg ggccgctcgg tgggacggaa gcgtgtggag agaccgccaa
      721 gggctgtagt ctgggtccgc gagcaaggtt gccctgaact gggggttggg gggagcgcag
      781 caaaatggcg gctgttcccg agtcttgaat ggaagacgct tgtgaggcgg gctgtgaggt
      841 cgttgaaaca aggtgggggg catggtgggc ggcaagaacc caaggtcttg aggccttcgc
      901 taatgcggga aagctcttat tcgggtgaga tgggctgggg caccatctgg ggaccctgac
      961 gtgaagtttg tcactgactg gagaactcgg tttgtcgtct gttgcggggg cggcagttat
     1021 ggcggtgccg ttgggcagtg cacccgtacc tttgggagcg cgcgccctcg tcgtgtcgtg
     1081 acgtcacccg ttctgttggc ttataatgca gggtggggcc acctgccggt aggtgtgcgg
     1141 taggcttttc tccgtcgcag gacgcagggt tcgggcctag ggtaggctct cctgaatcga
     1201 caggcgccgg acctctggtg aggggaggga taagtgaggc gtcagtttct ttggtcggtt
     1261 ttatgtacct atcttcttaa gtagctgaag ctccggtttt gaactatgcg ctcggggttg
     1321 gcgagtgtgt tttgtgaagt tttttaggca ccttttgaaa tgtaatcatt tgggtcaata
     1381 tgtaattttc agtgttagac tagtaaattg tccgctaaat tctggccgtt tttggctttt
     1441 ttgttagacg gccaactttt gtatacaaag tgggccacca tgggaatccc gctgctgaac
     1501 gctaaacaga ttctgggaca gctggacggc gggagcgtgg cagtcctgcc tgtggtcgac
     1561 tccaccaatc agtacctgct ggatcgaatc ggcgagctga agagtgggga tgcttgcatt
     1621 gcagaatatc agcaggcagg gagaggaagc agagggagga aatggttctc tccttttgga
     1681 gctaacctgt acctgagtat gttttggcgc ctgaagcggg gaccagcagc aatcggcctg
     1741 ggcccggtca tcggaattgt catggcagaa gcgctgcgaa agctgggagc agacaaggtg
     1801 cgagtcaaat ggcccaatga cctgtatctg caggatagaa agctggcagg catcctggtg
     1861 gagctggccg gaataacagg cgatgctgca cagatcgtca ttggcgccgg gattaacgtg
     1921 gctatgaggc gcgtggagga aagcgtggtc aatcagggct ggatcacact gcaggaagca
     1981 gggattaacc tggacaggaa tactctggcc gctatgctga tccgagagct gcgggcagcc
     2041 ctggaactgt tcgagcagga aggcctggct ccatatctgt cacggtggga gaagctggat
     2101 aacttcatca atagacccgt gaagctgatc attggggaca aagagatttt cgggattagc
     2161 cgggggattg ataaacaggg agccctgctg ctggaacagg acggagttat caaaccctgg
     2221 atgggcggag aaatcagtct gcggtctgcc gaaaaggcta gcggcaagcc catccccaac
     2281 cccctgctgg gcctggacag caccacaagt ttgtacaaaa aagcaggctc caccatggta
     2341 tcttctcaaa agttggaaaa acctatagag atgggcagta gcgaacccct tcccatcgca
     2401 gatggtgaca ggaggaggaa gaagaagcgg aggggccggg ccactgactc cttgccagga
     2461 aagtttgaag atatgtacaa gctgacctct gaattgcttg gagagggagc ctatgccaaa
     2521 gttcaaggtg ccgtgagcct acagaatggc aaagagtatg ccgtcaaaat catcgagaaa
     2581 caagcagggc acagtcggag tagggtgttt cgagaggtgg agacgctgta tcagtgtcag
     2641 ggaaacaaga acattttgga gctgattgag ttctttgaag atgacacaag gttttacttg
     2701 gtctttgaga aattgcaagg aggttccatc ttagcccaca tccagaagca aaagcacttc
     2761 aatgagcgag aagccagccg agtggtgcgg gacgttgctg ctgcccttga cttcctgcat
     2821 accaaagaca aagtctctct ctgtcaccta ggctggagtg ctatggcgcc atcagggctc
     2881 actgcagccc caacctccct gggctccagt gatcctccca cctcagcctc ccaagtagct
     2941 gggactacag gcattgctca tcgtgatctg aaaccagaaa atatattgtg tgaatctcca
     3001 gaaaaggtgt ctccagtgaa aatctgtgac tttgacttgg gcagtgggat gaaactgaac
     3061 aactcctgta cccccataac cacaccagag ctgaccaccc catgtggctc tgcagaatac
     3121 atggcccctg aggtagtgga ggtcttcacg gaccaggcca cattctacga caagcgctgt
     3181 gacctgtgga gcctgggcgt ggtcctctac atcatgctga gtggctaccc acccttcgtg
     3241 ggtcactgcg gggccgactg tggctgggac cggggcgagg tctgcagggt gtgccagaac
     3301 aagctgtttg aaagcatcca ggaaggcaag tatgagtttc ctgacaagga ctgggcacac
     3361 atctccagtg aagccaaaga cctcatctcc aagctcctgg tgcgagatgc aaagcagaga
     3421 cttagcgccg cccaagttct gcagcaccca tgggtgcagg ggcaagctcc agaaaaggga
     3481 ctccccacgc cgcaagtcct ccagaggaac agcagcacaa tggacctgac gctcttcgca
     3541 gctgaggcca tcgcccttaa ccgccagcta tctcagcacg aagagaacga actagcagag
     3601 gagccagagg cactagctga tggcctctgc tccatgaagc tttcccctcc ctgcaagtca
     3661 cgcctggccc ggagacgggc cctggcccag gcaggccgtg gtgaagacag gagcccgccc
     3721 acagcactct aggacccagc tttcttgtac aaagtggtga tatccagcac agtggcggcc
     3781 gctcgacaat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
     3841 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc
     3901 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga
     3961 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac
     4021 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc
     4081 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc
     4141 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aagctgacgt cctttccatg
     4201 gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc
     4261 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc
     4321 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctgg
     4381 aattctaccg ggtaggggag gcgcttttcc caaggcagtc tggagcatgc gctttagcag
     4441 ccccgctggg cacttggcgc tacacaagtg gcctctggcc tcgcacacat tccacatcca
     4501 ccggtaggcg ccaaccggct ccgttctttg gtggcccctt cgcgccacct tctactcctc
     4561 ccctagtcag gaagttcccc cccgccccgc agctcgcgtc gtgcaggacg tgacaaatgg
     4621 aagtagcacg tctcactagt ctcgtgcaga tggacagcac cgctgagcaa tggaagcggg
     4681 taggcctttg gggcagcggc caatagcagc tttgctcctt cgctttctgg gctcagaggc
     4741 tgggaagggg tgggtccggg ggcgggctca ggggcgggct caggggcggg gcgggcgccc
     4801 gaaggtcctc cggaggcccg gcattctgca cgcttcaaaa gcgcacgtct gccgcgctgt
     4861 tctcctcttc ctcatctccg ggcctttcga cctgcagccc aagcttacca tgaccgagta
     4921 caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc
     4981 cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga
     5041 gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt
     5101 gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc
     5161 gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc
     5221 cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt
     5281 cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt
     5341 gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc
     5401 gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt
     5461 gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgac gcccgcccca
     5521 cgacccgcag cgcccgaccg aaaggagcgc acgaccccat gcatctcgag ggcccggtac
     5581 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg
     5641 ggggactgga agggctagct cactcccaac gaagacaaga tctgcttttt gcttgtactg
     5701 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     5761 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     5821 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     5881 gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag
     5941 agagtgagag gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     6001 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     6061 tcaatgtatc ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccatcccgcc
     6121 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     6181 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
     6241 tggaggccta gggacgtacc caattcgccc tatagtgagt cgtattacgc gcgctcactg
     6301 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt
     6361 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct
     6421 tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc
     6481 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     6541 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     6601 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     6661 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     6721 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     6781 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
     6841 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg
     6901 cttacaattt aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     6961 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     7021 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     7081 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     7141 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     7201 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     7261 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     7321 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     7381 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     7441 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     7501 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     7561 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     7621 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     7681 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     7741 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     7801 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     7861 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     7921 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     7981 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     8041 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     8101 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     8161 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     8221 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     8281 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     8341 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     8401 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     8461 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     8521 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     8581 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     8641 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     8701 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     8761 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     8821 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     8881 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     8941 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     9001 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
     9061 accatgatta cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctgc
     9121 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta
     9181 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg
     9241 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac
     9301 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc
     9361 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     9421 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     9481 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg
     9541 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact
     9601 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa
     9661 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg
     9721 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat
     9781 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc
     9841 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag
     9901 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat
     9961 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac
    10021 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat
    10081 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg
    10141 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat
    10201 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat
    10261 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt
    10321 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca
    10381 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat
    10441 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag
    10501 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat
    10561 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa
    10621 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat
    10681 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt
    10741 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt
    10801 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga
    10861 ag
//
