LOCUS       sequence-335746-       10712 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway destination clone of MKNK2 (human) tagged with N-terminal 
            miniTurbo-V5 for generating protein-proximity networks using 
            proximity-dependent biotinylation proteomics.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10712)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 10712)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p663-UBC-miniTurbo-V5-MKNK2_IDG-K
FEATURES             Location/Qualifiers
     source          1..10712
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    108..224
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    258..278
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        278..1489
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     1326..1345
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    complement(1493..1513)
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     regulatory      1514..1523
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1526..2296
                     /codon_start=1
                     /product="N-terminally truncated mutant of the E. coli 
                     biotin ligase BioID engineered for improved catalytic 
                     efficiency (Branon et al., 2018)"
                     /label=miniTurbo
                     /translation="IPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGDACI
                     AEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLGADK
                     VRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWITLQ
                     EAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIF
                     GISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     CDS             2303..2344
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    2345..2369
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3624..3648)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    3678..4266
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3731..3751)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4149..4160)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        4280..4779
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4315..4338)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4712..4731
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             4800..5399
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4800..4819)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5296..5316
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             5537..5770
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5842..5963
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5879..5898)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5933..5952
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6003..6138
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6158..6177)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6159..6177)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6187..6204)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6187..6203)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6196..6218)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6345..6800
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6432..6451)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6642..6663
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6826..6930
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6931..6999,7000..7791)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      7962..8550
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8451..8470
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8704..8721
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8838..8859
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8874..8879,8880..8897,8898..8904)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8912..8928
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8917..8939
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8936..8952
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8936..8952
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8971..8991
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8973..8991
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        9019..9245
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             9246..9426
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9473..9598
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10091..10324
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10509..10553
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga
       61 gacagatcca ttcgattagt gaacggatct cgacggtatc ggttaacttt taaaagaaaa
      121 ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata
      181 caaactaaag aattacaaaa acaaattaca aaattcaaaa ttttatcgtc cagtgtggtg
      241 gaattctgca gatatcacaa ctttgtatag aaaagttggc ctccgcgccg ggttttggcg
      301 cctcccgcgg gcgcccccct cctcacggcg agcgctgcca cgtcagacga agggcgcagc
      361 gagcgtcctg atccttccgc ccggacgctc aggacagcgg cccgctgctc ataagactcg
      421 gccttagaac cccagtatca gcagaaggac attttaggac gggacttggg tgactctagg
      481 gcactggttt tctttccaga gagcggaaca ggcgaggaaa agtagtccct tctcggcgat
      541 tctgcggagg gatctccgtg gggcggtgaa cgccgatgat tatataagga cgcgccgggt
      601 gtggcacagc tagttccgtc gcagccggga tttgggtcgc ggttcttgtt tgtggatcgc
      661 tgtgatcgtc acttggtgag tagcgggctg ctgggctggc cggggctttc gtggccgccg
      721 ggccgctcgg tgggacggaa gcgtgtggag agaccgccaa gggctgtagt ctgggtccgc
      781 gagcaaggtt gccctgaact gggggttggg gggagcgcag caaaatggcg gctgttcccg
      841 agtcttgaat ggaagacgct tgtgaggcgg gctgtgaggt cgttgaaaca aggtgggggg
      901 catggtgggc ggcaagaacc caaggtcttg aggccttcgc taatgcggga aagctcttat
      961 tcgggtgaga tgggctgggg caccatctgg ggaccctgac gtgaagtttg tcactgactg
     1021 gagaactcgg tttgtcgtct gttgcggggg cggcagttat ggcggtgccg ttgggcagtg
     1081 cacccgtacc tttgggagcg cgcgccctcg tcgtgtcgtg acgtcacccg ttctgttggc
     1141 ttataatgca gggtggggcc acctgccggt aggtgtgcgg taggcttttc tccgtcgcag
     1201 gacgcagggt tcgggcctag ggtaggctct cctgaatcga caggcgccgg acctctggtg
     1261 aggggaggga taagtgaggc gtcagtttct ttggtcggtt ttatgtacct atcttcttaa
     1321 gtagctgaag ctccggtttt gaactatgcg ctcggggttg gcgagtgtgt tttgtgaagt
     1381 tttttaggca ccttttgaaa tgtaatcatt tgggtcaata tgtaattttc agtgttagac
     1441 tagtaaattg tccgctaaat tctggccgtt tttggctttt ttgttagacg gccaactttt
     1501 gtatacaaag tgggccacca tgggaatccc gctgctgaac gctaaacaga ttctgggaca
     1561 gctggacggc gggagcgtgg cagtcctgcc tgtggtcgac tccaccaatc agtacctgct
     1621 ggatcgaatc ggcgagctga agagtgggga tgcttgcatt gcagaatatc agcaggcagg
     1681 gagaggaagc agagggagga aatggttctc tccttttgga gctaacctgt acctgagtat
     1741 gttttggcgc ctgaagcggg gaccagcagc aatcggcctg ggcccggtca tcggaattgt
     1801 catggcagaa gcgctgcgaa agctgggagc agacaaggtg cgagtcaaat ggcccaatga
     1861 cctgtatctg caggatagaa agctggcagg catcctggtg gagctggccg gaataacagg
     1921 cgatgctgca cagatcgtca ttggcgccgg gattaacgtg gctatgaggc gcgtggagga
     1981 aagcgtggtc aatcagggct ggatcacact gcaggaagca gggattaacc tggacaggaa
     2041 tactctggcc gctatgctga tccgagagct gcgggcagcc ctggaactgt tcgagcagga
     2101 aggcctggct ccatatctgt cacggtggga gaagctggat aacttcatca atagacccgt
     2161 gaagctgatc attggggaca aagagatttt cgggattagc cgggggattg ataaacaggg
     2221 agccctgctg ctggaacagg acggagttat caaaccctgg atgggcggag aaatcagtct
     2281 gcggtctgcc gaaaaggcta gcggcaagcc catccccaac cccctgctgg gcctggacag
     2341 caccacaagt ttgtacaaaa aagcaggctc caccatggtg cagaagaaac cagccgaact
     2401 tcagggtttc caccgttcgt tcaaggggca gaaccccttc gagctggcct tctccctaga
     2461 ccagcccgac cacggagact ctgactttgg cctgcagtgc tcagcccgcc ctgacatgcc
     2521 cgccagccag cccattgaca tcccggacgc caagaagagg ggcaagaaga agaagcgcgg
     2581 ccgggccacc gacagcttct cgggcaggtt tgaagacgtc taccagctgc aggaagatgt
     2641 gctgggggag ggcgctcatg cccgagtgca gacctgcatc aacctgatca ccagccagga
     2701 gtacgccgtc aagatcattg agaagcagcc aggccacatt cggagcaggg ttttcaggga
     2761 ggtggagatg ctgtaccagt gccagggaca caggaacgtc ctagagctga ttgagttctt
     2821 cgaggaggag gaccgcttct acctggtgtt tgagaagatg cggggaggct ccatcctgag
     2881 ccacatccac aagcgccggc acttcaacga gctggaggcc agcgtggtgg tgcaggacgt
     2941 ggccagcgcc ttggactttc tgcataacaa aggcatcgcc cacagggacc taaagccgga
     3001 aaacatcctc tgtgagcacc ccaaccaggt ctcccccgtg aagatctgtg acttcgacct
     3061 gggcagcggc atcaaactca acggggactg ctcccctatc tccaccccgg agctgctcac
     3121 tccgtgcggc tcggcggagt acatggcccc ggaggtagtg gaggccttca gcgaggaggc
     3181 tagcatctac gacaagcgct gcgacctgtg gagcctgggc gtcatcttgt atatcctact
     3241 cagcggctac ccgcccttcg tgggccgctg tggcagcgac tgcggctggg accgcggcga
     3301 ggcctgccct gcctgccaga acatgctgtt tgagagcatc caggagggca agtacgagtt
     3361 ccccgacaag gactgggccc acatctcctg cgctgccaaa gacctcatct ccaagctgct
     3421 ggtccgtgac gccaagcaga ggctgagtgc cgcccaagtc ctgcagcacc cctgggttca
     3481 ggggtgcgcc ccggagaaca ccttgcccac tcccatggtc ctgcagaggt gggacagtca
     3541 cttcctcctc cctccccacc cctgtcgcat ccacgtgcga cctggaggac tggtcagaac
     3601 cgttactgtg aatgagtgaa tccacccagc tttcttgtac aaagtggtga tatccagcac
     3661 agtggcggcc gctcgacaat caacctctgg attacaaaat ttgtgaaaga ttgactggta
     3721 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc
     3781 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt
     3841 ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg
     3901 ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt
     3961 tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct
     4021 ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aagctgacgt
     4081 cctttccatg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct
     4141 acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc
     4201 ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct
     4261 ccccgcctgg aattctaccg ggtaggggag gcgcttttcc caaggcagtc tggagcatgc
     4321 gctttagcag ccccgctggg cacttggcgc tacacaagtg gcctctggcc tcgcacacat
     4381 tccacatcca ccggtaggcg ccaaccggct ccgttctttg gtggcccctt cgcgccacct
     4441 tctactcctc ccctagtcag gaagttcccc cccgccccgc agctcgcgtc gtgcaggacg
     4501 tgacaaatgg aagtagcacg tctcactagt ctcgtgcaga tggacagcac cgctgagcaa
     4561 tggaagcggg taggcctttg gggcagcggc caatagcagc tttgctcctt cgctttctgg
     4621 gctcagaggc tgggaagggg tgggtccggg ggcgggctca ggggcgggct caggggcggg
     4681 gcgggcgccc gaaggtcctc cggaggcccg gcattctgca cgcttcaaaa gcgcacgtct
     4741 gccgcgctgt tctcctcttc ctcatctccg ggcctttcga cctgcagccc aagcttacca
     4801 tgaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac
     4861 gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gatccggacc
     4921 gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca
     4981 tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga
     5041 gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt
     5101 cccggctggc cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc
     5161 ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca
     5221 gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg
     5281 agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg
     5341 acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgac
     5401 gcccgcccca cgacccgcag cgcccgaccg aaaggagcgc acgaccccat gcatctcgag
     5461 ggcccggtac ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta
     5521 aaagaaaagg ggggactgga agggctagct cactcccaac gaagacaaga tctgcttttt
     5581 gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
     5641 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
     5701 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
     5761 atctctagca gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa
     5821 tgaatatcag agagtgagag gaacttgttt attgcagctt ataatggtta caaataaagc
     5881 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     5941 tccaaactca tcaatgtatc ttatcatgtc tggctctagc tatcccgccc ctaactccgc
     6001 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
     6061 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag
     6121 gaggcttttt tggaggccta gggacgtacc caattcgccc tatagtgagt cgtattacgc
     6181 gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact
     6241 taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac
     6301 cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg
     6361 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
     6421 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
     6481 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
     6541 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac
     6601 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
     6661 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat
     6721 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa
     6781 aatattaacg cttacaattt aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     6841 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     6901 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     6961 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     7021 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     7081 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     7141 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
     7201 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     7261 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     7321 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     7381 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     7441 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
     7501 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     7561 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     7621 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     7681 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     7741 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     7801 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     7861 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     7921 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     7981 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     8041 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     8101 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     8161 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     8221 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     8281 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     8341 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     8401 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     8461 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     8521 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     8581 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     8641 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     8701 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
     8761 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc
     8821 agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac
     8881 tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga
     8941 aacagctatg accatgatta cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag
     9001 ctggagctgc aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa
     9061 cgatgagtta gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg
     9121 aagtaaggtg gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg
     9181 gacgaaccac tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca
     9241 taaacgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
     9301 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
     9361 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
     9421 ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg
     9481 acgcaggact cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag
     9541 tacgccaaaa attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag
     9601 tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa
     9661 gaaaaaatat aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt
     9721 taatcctggc ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc
     9781 atcccttcag acaggatcag aagaacttag atcattatat aatacagtag caaccctcta
     9841 ttgtgtgcat caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga
     9901 agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag
     9961 gaggagatat gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg
    10021 aaccattagg agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag
    10081 cagtgggaat aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg
    10141 cagcgtcaat gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc
    10201 agaacaattt gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg
    10261 gcatcaagca gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc
    10321 tcctggggat ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg
    10381 ctagttggag taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg
    10441 acagagaaat taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc
    10501 agcaagaaaa gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt
    10561 ggtttaacat aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct
    10621 tggtaggttt aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat
    10681 attcaccatt atcgtttcag acccacctcc ca
//
