LOCUS       sequence-33576-e         724 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 724)
  AUTHORS   Rothenfusser S, Goutagny N, DiPerna G, Gong M, Monks BG, 
            Schoenemeyer A, Yamamoto M, Akira S, Fitzgerald KA
  TITLE     The RNA helicase Lgp2 inhibits TLR-independent sensing of viral 
            replication by retinoic acid-inducible gene-I.
  JOURNAL   J Immunol. 2005 Oct 15. 175(8):5260-8.
  PUBMED    16210631
REFERENCE   2  (bases 1 to 724)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Immunol. 
            2005 Oct 15. 175(8):5260-8."
COMMENT     Sequence Label: pEF-BOS MDA5
FEATURES             Location/Qualifiers
     source          1..724
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(68..85)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(86..109)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(119..130)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 acgtgggaga cctgatactc tcaagggtcc caggtgggga ccctcactct agagtcgcgg
       61 ccgctcagtg gtggtggtgg tggtgcttgt catcgtcgtc cttgtagtcc ttgacgatcc
      121 ttccttcaat ggatccatcc tcatcactaa ataaacagca ttctgaatag tcaagattgg
      181 gaaatgtgat aggtaattct acccactttt tgtattgttt ctttgttgaa ttatttttga
      241 aaaccactac aaaattcctt attttgagac aaggcaaatc taagcctttg tgcaccatca
      301 ttgttcccca agcctggcca catttgcaga tgatttcacc atttatttga tagtcggcac
      361 acttcttttg cagtgctttg ttttctctta caatgtaaag ttccttgaat tctggggtca
      421 tattgacgtg atgcattttc tcaattacat ggatatcttc cccagaacag gctagcacac
      481 tgcagttttt gcaaaggaaa gttattagtg atgggttatt cttgtaatgc ttggcaatat
      541 ttctcttggt tttcattttc ttttccatta tactttgcat ctgtaattcc aaaatcttat
      601 gagcatactc ctctggtttc atattttgaa cacaatgtat agctttatac atcatcttct
      661 ctcggaaatc attaactgtc tcatgttcga taactcctga accactgtga gcaaccagga
      721 cgta
//
