LOCUS       sequence-335762-       10097 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A C-terminal APEX2 fusion to the mouse perilipin-2 protein PLIN2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10097)
  AUTHORS   Bersuker K, Peterson CWH, To M, Sahl SJ, Savikhin V, Grossman EA, 
            Nomura DK, Olzmann JA
  TITLE     A Proximity Labeling Strategy Provides Insights into the Composition
            and Dynamics of Lipid Droplet Proteomes.
  JOURNAL   Dev Cell. 2018 Jan 8;44(1):97-112.e7. doi: 
            10.1016/j.devcel.2017.11.020. Epub 2017 Dec 21.
  PUBMED    29275994
REFERENCE   2  (bases 1 to 10097)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2017.11.020"; journalName: "Dev Cell"; date: 
            "2018-01-8- 8"; volume: "44"; issue: "1"; pages: "97-112.e7"
COMMENT     Sequence Label: PLIN2-V5-APEX2
FEATURES             Location/Qualifiers
     source          1..10097
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..227
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             228..408
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    455..580
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1073..1306
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1491..1535
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1802..1918
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        1941..2244
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2245..2448
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2399..2419
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    2450..2468
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2471..2489
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2530..2554
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      2603..2612
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3893..3934
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             3935..4681
                     /codon_start=1
                     /product="soybean ascorbate peroxidase, engineered to serve
                     as a monomeric tag for electron microscopy and live-cell 
                     proteomics (Lam et al., 2015)"
                     /label=APEX2
                     /note="more active than the original APEX due to the A134P 
                     mutation"
                     /translation="GKSYPTVSADYQDAVEKAKKKLRGFIAEKRCAPLMLRLAFHSAGT
                     FDKGTKTGGPFGTIKHPAELAHSANNGLDIAVRLLEPLKAEFPILSYADFYQLAGVVAV
                     EVTGGPKVPFHPGREDKPEPPPEGRLPDPTKGSDHLRDVFGKAMGLTDQDIVALSGGHT
                     IGAAHKERSGFEGPWTSNPLIFDNSYFTELLSGEKEGLLQLPSDKALLSDPVFRPLVDK
                     YAADEDAFFADYAEAHQKLSELGFADA"
     protein_bind    complement(4702..4726)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    4757..5345
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4810..4830)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5228..5239)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        5359..5858
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5394..5417)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5791..5810
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             5879..6478
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5879..5898)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6375..6395
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             6616..6849
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6921..7042
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6958..6977)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7012..7031
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7082..7217
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7237..7256)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7238..7256)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7266..7283)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7266..7282)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7275..7297)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7424..7879
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7511..7530)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7721..7742
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7905..8009
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8010..8078,8079..8870)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8228..8247)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9041..9629
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9530..9549
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9783..9800
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9917..9938
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9953..9958,9959..9976,9977..9983)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9991..10007
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9996..10018
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10015..10031
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10015..10031
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10050..10070
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10052..10070
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg
       61 ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc
      121 gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc
      181 cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt
      241 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
      301 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
      361 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
      421 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
      481 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
      541 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
      601 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
      661 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
      721 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
      781 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
      841 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
      901 gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca
      961 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     1021 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     1081 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     1141 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     1201 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     1261 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     1321 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     1381 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     1441 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     1501 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     1561 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
     1621 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
     1681 agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg
     1741 gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcggttaa
     1801 cttttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa
     1861 tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaattc aaaattttat
     1921 cgataagctt gggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
     1981 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
     2041 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
     2101 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
     2161 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
     2221 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
     2281 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
     2341 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
     2401 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctct ccctatcagt
     2461 gatagagatc tccctatcag tgatagagat cgtcgactag tccagtgtgg tggaattctg
     2521 cagatatcaa caagtttgta caaaaaagca ggctttaaag gaaccaattc agtcgactgg
     2581 atccggtacc gaattcgcgg ccgccaccat ggcagcagca gtagtggacc cgcaacagag
     2641 cgtggtgatg agagtggcca acctgccctt ggtgagctct acctacgacc ttgtgtcctc
     2701 cgcttatgtc agtacaaagg atcagtaccc gtatttgaga tccgtgtgtg agatggccga
     2761 gaagggcgtg aagaccgtga cctctgcggc catgacaagt gccctgccca tcatccagaa
     2821 gctggagcca caaattgcgg ttgccaatac ctatgcctgc aaggggctag acaggatgga
     2881 ggaaagactg cctattctga accagccaac gtccgagatt gttgccagtg ccagaggtgc
     2941 cgtaactggg gcgaaggatg tggtgacgac taccatggct ggagccaagg attctgtagc
     3001 cagcacagtc tcaggggtgg tggataagac caaaggagca gtgactggca gcgtggaaag
     3061 gaccaagtct gtggtcaatg gcagcatcaa tacagttttg gggatggtgc agttcatgaa
     3121 cagtggagta gataatgcca tcaccaagtc ggagatgctg gtagaccagt acttccctct
     3181 cactcaggag gagctggaga tggaagcaaa aaaggtggaa ggatttgata tggttcagaa
     3241 gccgagcaac tatgaacggc tggagtccct gtctaccaag ctctgctctc gggcttatca
     3301 ccaggctctc agcagggtta aagaggccaa acaaaagagc caggagacca tttctcagct
     3361 ccactccact gtccacctga ttgaattcgc caggaagaat atgcacagtg ccaaccagaa
     3421 aattcagggt gctcaggata agctctatgt ctcgtgggtg gagtggaaga gaagcatcgg
     3481 ctacgacgac accgatgagt cccactgtgt tgagcacatc gagtcacgta ctctggctat
     3541 cgcccgcaac ctgacccagc agctccagac tacatgccag actgtcctgg tcaacgccca
     3601 agggttacca cagaacattc aagatcaggc caaacacttg ggggtgatgg caggcgacat
     3661 ctactccgta ttccgcaatg ctgcctcctt taaggaagtg tccgatggcg tcctcacatc
     3721 tagcaagggg cagctgcaga aaatgaagga atccttagat gaagttatgg attactttgt
     3781 taacaacacg cctctcaact ggctggtagg tcccttttat cctcagtcta ccgaggtgaa
     3841 caaggccagc ctgaaggtcc agcagtctga ggtcaaagct cagaaggatc caggcaagcc
     3901 catccccaac cccctgctgg gcctggacag caccggaaag tcttacccaa ctgtgagtgc
     3961 tgattaccag gacgccgttg agaaggcgaa gaagaagctc agaggcttca tcgctgagaa
     4021 gagatgcgct cctctaatgc tccgtttggc attccactct gctggaacct ttgacaaggg
     4081 cacgaagacc ggtggaccct tcggaaccat caagcaccct gccgaactgg ctcacagcgc
     4141 taacaacggt cttgacatcg ctgttaggct tttggagcca ctcaaggcgg agttccctat
     4201 tttgagctac gccgatttct accagttggc tggcgttgtt gccgttgagg tcacgggtgg
     4261 acctaaggtt ccattccacc ctggaagaga ggacaagcct gagccaccac cagagggtcg
     4321 cttgcccgat cccactaagg gttctgacca tttgagagat gtgtttggca aagctatggg
     4381 gcttactgac caagatatcg ttgctctatc tgggggtcac actattggag ctgcacacaa
     4441 ggagcgttct ggatttgagg gtccctggac ctctaatcct cttattttcg acaactcata
     4501 cttcacggag ttgttgagtg gtgagaagga aggtctcctt cagctacctt ctgacaaggc
     4561 tcttttgtct gaccctgtat tccgccctct cgttgataaa tatgcagcgg acgaagatgc
     4621 cttctttgct gattacgctg aggctcacca aaagctttcc gagcttgggt ttgctgatgc
     4681 ctaatgactc gagatatcta gacccagctt tcttgtacaa agtggttgat atccagcaca
     4741 gtggcggccg ctcgacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat
     4801 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca
     4861 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc
     4921 tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc
     4981 tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt
     5041 cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg
     5101 gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc
     5161 ctttccatgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta
     5221 cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg
     5281 gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc
     5341 cccgcctgga attctaccgg gtaggggagg cgcttttccc aaggcagtct ggagcatgcg
     5401 ctttagcagc cccgctgggc acttggcgct acacaagtgg cctctggcct cgcacacatt
     5461 ccacatccac cggtaggcgc caaccggctc cgttctttgg tggccccttc gcgccacctt
     5521 ctactcctcc cctagtcagg aagttccccc ccgccccgca gctcgcgtcg tgcaggacgt
     5581 gacaaatgga agtagcacgt ctcactagtc tcgtgcagat ggacagcacc gctgagcaat
     5641 ggaagcgggt aggcctttgg ggcagcggcc aatagcagct ttgctccttc gctttctggg
     5701 ctcagaggct gggaaggggt gggtccgggg gcgggctcag gggcgggctc aggggcgggg
     5761 cgggcgcccg aaggtcctcc ggaggcccgg cattctgcac gcttcaaaag cgcacgtctg
     5821 ccgcgctgtt ctcctcttcc tcatctccgg gcctttcgac ctgcagccca agcttaccat
     5881 gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg
     5941 caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg
     6001 ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat
     6061 cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag
     6121 cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc
     6181 ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc
     6241 cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag
     6301 cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga
     6361 gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga
     6421 cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgacg
     6481 cccgccccac gacccgcagc gcccgaccga aaggagcgca cgaccccatg catctcgagg
     6541 gcccggtacc tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa
     6601 aagaaaaggg gggactggaa gggctagctc actcccaacg aagacaagat ctgctttttg
     6661 cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     6721 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     6781 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     6841 tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat
     6901 gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca
     6961 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     7021 ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc
     7081 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     7141 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg
     7201 aggctttttt ggaggcctag ggacgtaccc aattcgccct atagtgagtc gtattacgcg
     7261 cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt
     7321 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
     7381 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc
     7441 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     7501 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     7561 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     7621 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     7681 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     7741 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
     7801 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa
     7861 atattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     7921 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     7981 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     8041 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     8101 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     8161 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     8221 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     8281 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     8341 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     8401 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     8461 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     8521 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     8581 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     8641 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     8701 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     8761 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     8821 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     8881 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     8941 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     9001 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     9061 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     9121 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     9181 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     9241 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     9301 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     9361 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     9421 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     9481 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     9541 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     9601 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     9661 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     9721 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     9781 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
     9841 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
     9901 gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact
     9961 ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa
    10021 acagctatga ccatgattac gccaagcgcg caattaaccc tcactaaagg gaacaaaagc
    10081 tggagctgca agcttaa
//
