LOCUS       sequence-335767-       11492 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway destination clone of PIP5K1C (human) tagged with N-terminal 
            miniTurbo-V5 for generating protein-proximity networks using 
            proximity-dependent biotinylation proteomics.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11492)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 11492)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p663-UBC-miniTurbo-V5-PIP5K1C_IDG-K
FEATURES             Location/Qualifiers
     source          1..11492
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    153..269
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    303..323
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        323..1534
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     1371..1390
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    complement(1538..1558)
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     regulatory      1559..1568
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1571..2341
                     /codon_start=1
                     /product="N-terminally truncated mutant of the E. coli 
                     biotin ligase BioID engineered for improved catalytic 
                     efficiency (Branon et al., 2018)"
                     /label=miniTurbo
                     /translation="IPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGDACI
                     AEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLGADK
                     VRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWITLQ
                     EAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIF
                     GISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     CDS             2348..2389
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    2390..2414
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4449..4473)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    4503..5091
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4556..4576)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4974..4985)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        5105..5604
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5140..5163)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5537..5556
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             5625..6224
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5625..5644)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6121..6141
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             6362..6595
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6667..6788
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6704..6723)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6758..6777
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6828..6963
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6983..7002)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6984..7002)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7012..7029)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7012..7028)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7021..7043)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7170..7625
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7257..7276)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7467..7488
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7651..7755
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7756..7824,7825..8616)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      8787..9375
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9276..9295
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9529..9546
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9663..9684
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9699..9704,9705..9722,9723..9729)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9737..9753
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9742..9764
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9761..9777
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9761..9777
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9796..9816
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9798..9816
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        9844..10070
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             10071..10251
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    10298..10423
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10916..11149
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11334..11378
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 gttaggcagg gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc
       61 gacaggcccg aaggaataga agaagaaggt ggagagagag acagagacag atccattcga
      121 ttagtgaacg gatctcgacg gtatcggtta acttttaaaa gaaaaggggg gattgggggg
      181 tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta
      241 caaaaacaaa ttacaaaatt caaaatttta tcgtccagtg tggtggaatt ctgcagatat
      301 cacaactttg tatagaaaag ttggcctccg cgccgggttt tggcgcctcc cgcgggcgcc
      361 cccctcctca cggcgagcgc tgccacgtca gacgaagggc gcagcgagcg tcctgatcct
      421 tccgcccgga cgctcaggac agcggcccgc tgctcataag actcggcctt agaaccccag
      481 tatcagcaga aggacatttt aggacgggac ttgggtgact ctagggcact ggttttcttt
      541 ccagagagcg gaacaggcga ggaaaagtag tcccttctcg gcgattctgc ggagggatct
      601 ccgtggggcg gtgaacgccg atgattatat aaggacgcgc cgggtgtggc acagctagtt
      661 ccgtcgcagc cgggatttgg gtcgcggttc ttgtttgtgg atcgctgtga tcgtcacttg
      721 gtgagtagcg ggctgctggg ctggccgggg ctttcgtggc cgccgggccg ctcggtggga
      781 cggaagcgtg tggagagacc gccaagggct gtagtctggg tccgcgagca aggttgccct
      841 gaactggggg ttggggggag cgcagcaaaa tggcggctgt tcccgagtct tgaatggaag
      901 acgcttgtga ggcgggctgt gaggtcgttg aaacaaggtg gggggcatgg tgggcggcaa
      961 gaacccaagg tcttgaggcc ttcgctaatg cgggaaagct cttattcggg tgagatgggc
     1021 tggggcacca tctggggacc ctgacgtgaa gtttgtcact gactggagaa ctcggtttgt
     1081 cgtctgttgc gggggcggca gttatggcgg tgccgttggg cagtgcaccc gtacctttgg
     1141 gagcgcgcgc cctcgtcgtg tcgtgacgtc acccgttctg ttggcttata atgcagggtg
     1201 gggccacctg ccggtaggtg tgcggtaggc ttttctccgt cgcaggacgc agggttcggg
     1261 cctagggtag gctctcctga atcgacaggc gccggacctc tggtgagggg agggataagt
     1321 gaggcgtcag tttctttggt cggttttatg tacctatctt cttaagtagc tgaagctccg
     1381 gttttgaact atgcgctcgg ggttggcgag tgtgttttgt gaagtttttt aggcaccttt
     1441 tgaaatgtaa tcatttgggt caatatgtaa ttttcagtgt tagactagta aattgtccgc
     1501 taaattctgg ccgtttttgg cttttttgtt agacggccaa cttttgtata caaagtgggc
     1561 caccatggga atcccgctgc tgaacgctaa acagattctg ggacagctgg acggcgggag
     1621 cgtggcagtc ctgcctgtgg tcgactccac caatcagtac ctgctggatc gaatcggcga
     1681 gctgaagagt ggggatgctt gcattgcaga atatcagcag gcagggagag gaagcagagg
     1741 gaggaaatgg ttctctcctt ttggagctaa cctgtacctg agtatgtttt ggcgcctgaa
     1801 gcggggacca gcagcaatcg gcctgggccc ggtcatcgga attgtcatgg cagaagcgct
     1861 gcgaaagctg ggagcagaca aggtgcgagt caaatggccc aatgacctgt atctgcagga
     1921 tagaaagctg gcaggcatcc tggtggagct ggccggaata acaggcgatg ctgcacagat
     1981 cgtcattggc gccgggatta acgtggctat gaggcgcgtg gaggaaagcg tggtcaatca
     2041 gggctggatc acactgcagg aagcagggat taacctggac aggaatactc tggccgctat
     2101 gctgatccga gagctgcggg cagccctgga actgttcgag caggaaggcc tggctccata
     2161 tctgtcacgg tgggagaagc tggataactt catcaataga cccgtgaagc tgatcattgg
     2221 ggacaaagag attttcggga ttagccgggg gattgataaa cagggagccc tgctgctgga
     2281 acaggacgga gttatcaaac cctggatggg cggagaaatc agtctgcggt ctgccgaaaa
     2341 ggctagcggc aagcccatcc ccaaccccct gctgggcctg gacagcacca caagtttgta
     2401 caaaaaagca gaagggccgt caaggcccac catggagctg gaggtaccgg acgaggcgga
     2461 gagcgctgag gcgggggccg tgccctcgga ggcggcgtgg gcggcagaga gcggggcggc
     2521 ggcaggtttg gctcagaaga aggcggcccc aacagaggtt ctgtccatga cggcacagcc
     2581 gggccctggc catgggaaga agttgggcca tcgaggtgtg gacgcatccg gcgaaaccac
     2641 ctacaagaag accacctcct ccaccctgaa gggtgccatc cagctgggca tcggctacac
     2701 cgtgggccac ctgagctcca agcccgaacg cgacgtgctc atgcaggact tctacgtggt
     2761 ggagagcatc ttcttcccca gcgaaggcag caacctcacc cccgcccacc acttccagga
     2821 cttccgcttc aagacctatg cacctgtcgc cttccgctac ttccgggagc tctttgggat
     2881 ccggccagat gattacttgt actccctgtg caatgagccg ctgatcgagc tgtccaaccc
     2941 gggcgccagt ggctccctct tctacgtcac cagcgacgac gagttcatca tcaagaccgt
     3001 catgcacaag gaggccgagt tcctgcagaa gctgctccct ggctactaca tgaacctcaa
     3061 ccagaacccg cggacgctgc tgcccaagtt ctatgggctg tactgcgtgc agtcgggggg
     3121 caagaacatc cgcgtcgtgg tcatgaacaa catcctgccc cgcgtggtca agatgcacct
     3181 caagttcgac ctcaagggct ccacctacaa gcggcgcgcc agcaagaagg agaaggagaa
     3241 gagcttcccc acctacaagg acctggactt catgcaggac atgcccgagg ggctcctgct
     3301 ggacgccgac accttcagcg ccctggtcaa gacgctgcag cgggactgcc tggtcctgga
     3361 aagtttcaag atcatggact acagcctgct gctgggcgtg cacaacatcg accagcacga
     3421 gcgcgagcgg caggcgcagg gcgcccagag cacctcagat gagaagcggc ctgtgggcca
     3481 gaaggcgctc tactccacgg ccatggagtc catccagggt ggcgccgcgc gcggggaggc
     3541 catcgaatcg gatgacacga tgggcgggat ccccgctgtg aacggccgcg gggagcggct
     3601 gctgctgcac attggcatca tcgacatcct gcagtcctac aggttcatca agaaactgga
     3661 gcacacctgg aaggccctcg tccacgatgg ggacacggtg tccgtccacc gccccagctt
     3721 ctatgccgag cgctttttca agttcatgag caacacggtc tttcggaaga actcctccct
     3781 gaagtcctcg ccctccaaga aggggcgcgg cggagccttg ctagctgtga aaccgctggg
     3841 gcccaccgct gccttctcgg ccagccagat ccctagcgag cgggaggagg cccagtacga
     3901 cctgcggggg gcccgcagct accccacgct ggaggacgaa ggccggcccg acctcctgcc
     3961 ctgcacgcca ccttctttcg aagaagccac tacagcctcc attgccacga ctctgtcatc
     4021 cacatccctc tccattcctg agcggtcccc ctcggagacg tcggagcagc cgcggtacag
     4081 gcggcgcaca cagtcgtctg gacaggatgg caggccgcag gaggagccac ccgcggaaga
     4141 ggatctgcag cagattacag tgcaggtgga gcctgcgtgc agcgtggaga ttgtggtccc
     4201 caaagaggag gacgcagggg tggaggcttc cccggccggt gcctctgctg ctgttgaagt
     4261 agaaactgcc agccaggcct cagacgagga gggcgcacct gccagccagg cctcggacga
     4321 ggaggacgcg cccgccaccg acatctactt tcccaccgat gagaggagct gggtgtactc
     4381 cccgctccac tatagcgccc aggccccccc ggcctccgac ggcgagagcg acacataggg
     4441 cctcatgggc ccagctttct tgtacaaagt ggtgatatcc agcacagtgg cggccgctcg
     4501 acaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg
     4561 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc
     4621 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt
     4681 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca
     4741 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc
     4801 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc
     4861 tgttgggcac tgacaattcc gtggtgttgt cggggaagct gacgtccttt ccatggctgc
     4921 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc
     4981 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc
     5041 ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg cctggaattc
     5101 taccgggtag gggaggcgct tttcccaagg cagtctggag catgcgcttt agcagccccg
     5161 ctgggcactt ggcgctacac aagtggcctc tggcctcgca cacattccac atccaccggt
     5221 aggcgccaac cggctccgtt ctttggtggc cccttcgcgc caccttctac tcctccccta
     5281 gtcaggaagt tcccccccgc cccgcagctc gcgtcgtgca ggacgtgaca aatggaagta
     5341 gcacgtctca ctagtctcgt gcagatggac agcaccgctg agcaatggaa gcgggtaggc
     5401 ctttggggca gcggccaata gcagctttgc tccttcgctt tctgggctca gaggctggga
     5461 aggggtgggt ccgggggcgg gctcaggggc gggctcaggg gcggggcggg cgcccgaagg
     5521 tcctccggag gcccggcatt ctgcacgctt caaaagcgca cgtctgccgc gctgttctcc
     5581 tcttcctcat ctccgggcct ttcgacctgc agcccaagct taccatgacc gagtacaagc
     5641 ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg
     5701 cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg
     5761 tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg
     5821 tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg
     5881 cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc
     5941 agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg
     6001 ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc
     6061 ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc
     6121 gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg
     6181 aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgacgcccg ccccacgacc
     6241 cgcagcgccc gaccgaaagg agcgcacgac cccatgcatc tcgagggccc ggtaccttta
     6301 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga
     6361 ctggaagggc tagctcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct
     6421 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     6481 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
     6541 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt
     6601 agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt
     6661 gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     6721 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     6781 gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccatc ccgcccctaa
     6841 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     6901 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     6961 gcctagggac gtacccaatt cgccctatag tgagtcgtat tacgcgcgct cactggccgt
     7021 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc
     7081 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca
     7141 acagttgcgc agcctgaatg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc
     7201 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
     7261 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     7321 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
     7381 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
     7441 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
     7501 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
     7561 aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac
     7621 aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     7681 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     7741 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     7801 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     7861 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     7921 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     7981 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
     8041 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     8101 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     8161 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     8221 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     8281 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
     8341 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     8401 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     8461 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     8521 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     8581 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     8641 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     8701 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     8761 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     8821 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     8881 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     8941 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     9001 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     9061 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     9121 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     9181 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     9241 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     9301 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     9361 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     9421 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     9481 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     9541 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
     9601 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
     9661 attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct
     9721 cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat
     9781 gattacgcca agcgcgcaat taaccctcac taaagggaac aaaagctgga gctgcaagct
     9841 taatgtagtc ttatgcaata ctcttgtagt cttgcaacat ggtaacgatg agttagcaac
     9901 atgccttaca aggagagaaa aagcaccgtg catgccgatt ggtggaagta aggtggtacg
     9961 atcgtgcctt attaggaagg caacagacgg gtctgacatg gattggacga accactgaat
    10021 tgccgcattg cagagatatt gtatttaagt gcctagctcg atacataaac gggtctctct
    10081 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
    10141 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
    10201 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc
    10261 gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct
    10321 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
    10381 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
    10441 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
    10501 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
    10561 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
    10621 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
    10681 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
    10741 taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga gatatgaggg
    10801 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
    10861 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
    10921 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc
    10981 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
    11041 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
    11101 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
    11161 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
    11221 aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca
    11281 attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg
    11341 aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa
    11401 attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa
    11461 tagtttttgc tgtactttct atagtgaata ga
//
