LOCUS       sequence-335768-       12137 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway destination clone of PKN3 (human) tagged with N-terminal 
            miniTurbo-V5 for generating protein-proximity networks using 
            proximity-dependent biotinylation proteomics.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12137)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 12137)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p663-UBC-miniTurbo-V5-PKN3_IDG-K
FEATURES             Location/Qualifiers
     source          1..12137
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        22..126
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(127..195,196..987)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1158..1746
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1647..1666
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1900..1917
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2034..2055
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2070..2075,2076..2093,2094..2100)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2108..2124
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2113..2135
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2132..2148
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2132..2148
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2167..2187
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2169..2187
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2215..2441
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2442..2622
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2669..2794
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3287..3520
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3705..3749
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4016..4132
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    4166..4186
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        4186..5397
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     5234..5253
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    complement(5401..5421)
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     regulatory      5422..5431
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5434..6204
                     /codon_start=1
                     /product="N-terminally truncated mutant of the E. coli 
                     biotin ligase BioID engineered for improved catalytic 
                     efficiency (Branon et al., 2018)"
                     /label=miniTurbo
                     /translation="IPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGDACI
                     AEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLGADK
                     VRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWITLQ
                     EAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIF
                     GISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     CDS             6211..6252
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    6253..6277
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(8957..8981)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    9011..9599
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9064..9084)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9482..9493)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        9613..10112
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(9648..9671)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     10045..10064
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             10133..10732
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(10133..10152)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     10629..10649
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             10870..11103
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    11175..11296
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11212..11231)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11266..11285
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      11336..11471
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(11491..11510)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(11492..11510)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(11520..11537)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(11520..11536)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11529..11551)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      11678..12133
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11765..11784)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11975..11996
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
       61 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
      121 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
      181 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
      241 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
      301 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
      361 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
      421 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
      481 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
      541 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
      601 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
      661 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
      721 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
      781 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
      841 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
      901 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
      961 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     1021 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     1081 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     1141 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     1201 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     1261 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     1321 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     1381 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     1441 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     1501 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     1561 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     1621 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     1681 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     1741 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     1801 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     1861 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     1921 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
     1981 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg
     2041 agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg
     2101 tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc
     2161 aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg agctgcaagc ttaatgtagt
     2221 cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa catgccttac
     2281 aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac gatcgtgcct
     2341 tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa ttgccgcatt
     2401 gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc tggttagacc
     2461 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     2521 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     2581 gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga
     2641 cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc
     2701 gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg
     2761 aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc
     2821 gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat
     2881 agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc
     2941 agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga
     3001 acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat
     3061 aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaccac
     3121 cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga
     3181 gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca
     3241 aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc
     3301 ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac
     3361 aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg
     3421 aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa
     3481 tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg
     3541 gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg
     3601 aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa
     3661 gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat
     3721 tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt
     3781 ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg
     3841 ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc
     3901 acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa ggtggagaga
     3961 gagacagaga cagatccatt cgattagtga acggatctcg acggtatcgg ttaactttta
     4021 aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa
     4081 cagacataca aactaaagaa ttacaaaaac aaattacaaa attcaaaatt ttatcgtcca
     4141 gtgtggtgga attctgcaga tatcacaact ttgtatagaa aagttggcct ccgcgccggg
     4201 ttttggcgcc tcccgcgggc gcccccctcc tcacggcgag cgctgccacg tcagacgaag
     4261 ggcgcagcga gcgtcctgat ccttccgccc ggacgctcag gacagcggcc cgctgctcat
     4321 aagactcggc cttagaaccc cagtatcagc agaaggacat tttaggacgg gacttgggtg
     4381 actctagggc actggttttc tttccagaga gcggaacagg cgaggaaaag tagtcccttc
     4441 tcggcgattc tgcggaggga tctccgtggg gcggtgaacg ccgatgatta tataaggacg
     4501 cgccgggtgt ggcacagcta gttccgtcgc agccgggatt tgggtcgcgg ttcttgtttg
     4561 tggatcgctg tgatcgtcac ttggtgagta gcgggctgct gggctggccg gggctttcgt
     4621 ggccgccggg ccgctcggtg ggacggaagc gtgtggagag accgccaagg gctgtagtct
     4681 gggtccgcga gcaaggttgc cctgaactgg gggttggggg gagcgcagca aaatggcggc
     4741 tgttcccgag tcttgaatgg aagacgcttg tgaggcgggc tgtgaggtcg ttgaaacaag
     4801 gtggggggca tggtgggcgg caagaaccca aggtcttgag gccttcgcta atgcgggaaa
     4861 gctcttattc gggtgagatg ggctggggca ccatctgggg accctgacgt gaagtttgtc
     4921 actgactgga gaactcggtt tgtcgtctgt tgcgggggcg gcagttatgg cggtgccgtt
     4981 gggcagtgca cccgtacctt tgggagcgcg cgccctcgtc gtgtcgtgac gtcacccgtt
     5041 ctgttggctt ataatgcagg gtggggccac ctgccggtag gtgtgcggta ggcttttctc
     5101 cgtcgcagga cgcagggttc gggcctaggg taggctctcc tgaatcgaca ggcgccggac
     5161 ctctggtgag gggagggata agtgaggcgt cagtttcttt ggtcggtttt atgtacctat
     5221 cttcttaagt agctgaagct ccggttttga actatgcgct cggggttggc gagtgtgttt
     5281 tgtgaagttt tttaggcacc ttttgaaatg taatcatttg ggtcaatatg taattttcag
     5341 tgttagacta gtaaattgtc cgctaaattc tggccgtttt tggctttttt gttagacggc
     5401 caacttttgt atacaaagtg ggccaccatg ggaatcccgc tgctgaacgc taaacagatt
     5461 ctgggacagc tggacggcgg gagcgtggca gtcctgcctg tggtcgactc caccaatcag
     5521 tacctgctgg atcgaatcgg cgagctgaag agtggggatg cttgcattgc agaatatcag
     5581 caggcaggga gaggaagcag agggaggaaa tggttctctc cttttggagc taacctgtac
     5641 ctgagtatgt tttggcgcct gaagcgggga ccagcagcaa tcggcctggg cccggtcatc
     5701 ggaattgtca tggcagaagc gctgcgaaag ctgggagcag acaaggtgcg agtcaaatgg
     5761 cccaatgacc tgtatctgca ggatagaaag ctggcaggca tcctggtgga gctggccgga
     5821 ataacaggcg atgctgcaca gatcgtcatt ggcgccggga ttaacgtggc tatgaggcgc
     5881 gtggaggaaa gcgtggtcaa tcagggctgg atcacactgc aggaagcagg gattaacctg
     5941 gacaggaata ctctggccgc tatgctgatc cgagagctgc gggcagccct ggaactgttc
     6001 gagcaggaag gcctggctcc atatctgtca cggtgggaga agctggataa cttcatcaat
     6061 agacccgtga agctgatcat tggggacaaa gagattttcg ggattagccg ggggattgat
     6121 aaacagggag ccctgctgct ggaacaggac ggagttatca aaccctggat gggcggagaa
     6181 atcagtctgc ggtctgccga aaaggctagc ggcaagccca tccccaaccc cctgctgggc
     6241 ctggacagca ccacaagttt gtacaaaaaa gcaggctcca ccatggagga gggggcgccg
     6301 cggcagcctg ggccgagcca gtggccccca gaggatgaga aggaggtgat ccgccgggcc
     6361 atccagaaag agctgaagat caaggagggg gtggagaacc tgcggcgcgt ggccacagac
     6421 cgccgccact tgggccatgt gcagcagctg ctgcggtcct ccaaccgccg cctggagcag
     6481 ctgcatggcg agctgcggga gctgcacgcc cgaatcctgc tgcccggccc tgggcctggc
     6541 ccagctgagc ctgtggcctc aggaccccgg ccgtgggcag agcagctcag ggctcggcac
     6601 ctagaggctc tccggaggca gctgcatgtg gagctgaagg tgaagcaggg ggctgagaac
     6661 atgacccaca cgtgcgccag tggcaccccc aaggagagga agctcctggc agctgcccag
     6721 cagatgctgc gggacagcca gctgaaggtg gccctgctgc ggatgaagat cagcagcctg
     6781 gaggccagtg ggtccccgga gccagggcct gagctgctgg cggaggagct acagcatcga
     6841 ctgcacgttg aggcagctgt ggctgagggc gccaagaacg tggtgaaact gcttagtagc
     6901 cggagaacac aggaccgcaa ggcactggct gaggcccagg cccagctaca ggagtcctct
     6961 cagaaactgg acctcctgcg cctggccttg gagcagctgc tggagcaact gcctcctgcc
     7021 caccctttgc gcagcagagt gacccgagag ttgcgggctg cggtgcctgg atacccccag
     7081 ccttcaggga cacctgtgaa gcccaccgcc ctaacaggga cactgcaggt ccgcctcctg
     7141 ggctgtgaac agttgctgac agccgtgcct gggcgctccc cagcggccgc actggccagc
     7201 agcccctccg agggctggct tcggaccaag gccaagcacc agcgtggccg aggcgagctt
     7261 gccagcgagg tgctggctgt gctaaaggtg gacaaccgtg ttgtggggca gacgggctgg
     7321 gggcaggtgg ccgaacagtc ctgggaccag acctttgtca tcccactgga gcgagcccgt
     7381 gagctggaga ttggggtaca ctggcgggac tggcggcagc tatgtggcgt ggccttcctg
     7441 agacttgagg acttcctgga caatgcctgt caccaactgt ccctcagcct ggtaccgcag
     7501 ggactgcttt ttgcccaggt gaccttctgc gatcctgtca ttgagaggcg gccccggctg
     7561 cagaggcagg aacgcatctt ctctaaacgc agaggccagg acttcctgag ggcttcgcag
     7621 atgaacctcg gcatggcggc ctgggggcgc ctcgtcatga acctgctgcc cccctgcagc
     7681 tccccgagca caatcagccc ccctaaagga tgccctcgga ccccaacaac actgcgagag
     7741 gcctctgacc ctgccactcc cagtaatttc ctgcccaaga agaccccctt gggtgaagag
     7801 atgacacccc cacccaagcc cccacgcctc tacctccccc aggagccaac atccgaggag
     7861 actccgcgca ccaaacgtcc ccatatggag cctaggactc gacgtgggcc atctccacca
     7921 gcctccccca ccaggaaacc ccctcggctt caggacttcc gctgcttagc tgtgctgggc
     7981 cggggacact ttgggaaggt cctcctggtc cagttcaagg ggacagggaa atactacgcc
     8041 atcaaagcac tgaagaagca ggaggtgctc agccgggacg agatagagag cctgtactgc
     8101 gagaagcgga tcctggaggc tgtgggctgc acagggcacc ctttcctgct ctccctcctt
     8161 gcctgcttcc agacctccag ccatgcctgc tttgtgactg agtttgtgcc tggtggtgac
     8221 ctcatgatgc agatccacga ggatgtcttc cccgagcccc aggcccgctt ctacgtggct
     8281 tgtgttgtcc tggggctgca gttcttacac gagaagaaga tcatttacag ggacctgaag
     8341 ttggataacc ttctgctgga tgcccaggga ttcctgaaga tcgcagactt tggactctgc
     8401 aaggaaggga tcggcttcgg ggaccggact agcaccttct gtggcacccc ggagttcctg
     8461 gctcccgagg tgctgaccca ggaggcatac acacgggctg tggactggtg ggggctgggt
     8521 gtgctgctct acgagatgct ggtgggtgag tgcccgttcc caggggacac agaggaagag
     8581 gtgtttgact gcatcgtcaa catggacgcc ccctaccccg gctttctgtc ggtgcaaggg
     8641 cttgagttca ttcagaagct cctccagaag tgcccggaga agcgcctcgg ggcaggtgag
     8701 caggatgccg aggagatcaa ggtccagcca ttcttcagga ccaccaactg gcaagccctg
     8761 ctcgcccgca ccatccagcc ccccttcgtg cctaccctgt gtggccctgc ggacctgcgc
     8821 tactttgagg gcgagttcac agggctgccg cctgccctga ccccacctgc accccacagc
     8881 ctcctcactg cccgccaaca ggccgccttc cgggacttcg actttgtgtc agagcgattc
     8941 ctggaaccct gaatccaccc agctttcttg tacaaagtgg tgatatccag cacagtggcg
     9001 gccgctcgac aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa
     9061 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat
     9121 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta
     9181 tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc
     9241 aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt
     9301 ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg
     9361 ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaagctga cgtcctttcc
     9421 atggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc
     9481 ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct
     9541 tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcc
     9601 tggaattcta ccgggtaggg gaggcgcttt tcccaaggca gtctggagca tgcgctttag
     9661 cagccccgct gggcacttgg cgctacacaa gtggcctctg gcctcgcaca cattccacat
     9721 ccaccggtag gcgccaaccg gctccgttct ttggtggccc cttcgcgcca ccttctactc
     9781 ctcccctagt caggaagttc ccccccgccc cgcagctcgc gtcgtgcagg acgtgacaaa
     9841 tggaagtagc acgtctcact agtctcgtgc agatggacag caccgctgag caatggaagc
     9901 gggtaggcct ttggggcagc ggccaatagc agctttgctc cttcgctttc tgggctcaga
     9961 ggctgggaag gggtgggtcc gggggcgggc tcaggggcgg gctcaggggc ggggcgggcg
    10021 cccgaaggtc ctccggaggc ccggcattct gcacgcttca aaagcgcacg tctgccgcgc
    10081 tgttctcctc ttcctcatct ccgggccttt cgacctgcag cccaagctta ccatgaccga
    10141 gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct
    10201 cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat
    10261 cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa
    10321 ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga
    10381 agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct
    10441 ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg
    10501 gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt
    10561 cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc
    10621 cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga
    10681 ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gacgcccgcc
    10741 ccacgacccg cagcgcccga ccgaaaggag cgcacgaccc catgcatctc gagggcccgg
    10801 tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa
    10861 aggggggact ggaagggcta gctcactccc aacgaagaca agatctgctt tttgcttgta
    10921 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
    10981 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
    11041 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
    11101 gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat
    11161 cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
    11221 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
    11281 tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc cgcccatccc
    11341 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
    11401 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
    11461 ttttggaggc ctagggacgt acccaattcg ccctatagtg agtcgtatta cgcgcgctca
    11521 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc
    11581 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc
    11641 ccttcccaac agttgcgcag cctgaatggc gaatgggacg cgccctgtag cggcgcatta
    11701 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
    11761 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
    11821 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
    11881 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
    11941 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
    12001 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
    12061 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta
    12121 acgcttacaa tttaggt
//
