LOCUS       sequence-335770-       10520 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway destination clone of PRKACG (human) tagged with N-terminal 
            miniTurbo-V5 for generating protein-proximity networks using 
            proximity-dependent biotinylation proteomics.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10520)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 10520)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p663-UBC-miniTurbo-V5-PRKACG_IDG-K
FEATURES             Location/Qualifiers
     source          1..10520
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    145..261
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    295..315
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        315..1526
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     1363..1382
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    complement(1530..1550)
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     regulatory      1551..1560
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1563..2333
                     /codon_start=1
                     /product="N-terminally truncated mutant of the E. coli 
                     biotin ligase BioID engineered for improved catalytic 
                     efficiency (Branon et al., 2018)"
                     /label=miniTurbo
                     /translation="IPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGDACI
                     AEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLGADK
                     VRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWITLQ
                     EAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIF
                     GISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     CDS             2340..2381
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    2382..2406
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3469..3493)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    3523..4111
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3576..3596)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3994..4005)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        4125..4624
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4160..4183)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4557..4576
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             4645..5244
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4645..4664)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5141..5161
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             5382..5615
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5687..5808
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5724..5743)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5778..5797
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5848..5983
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6003..6022)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6004..6022)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6032..6049)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6032..6048)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6041..6063)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6190..6645
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6277..6296)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6487..6508
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6671..6775
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6776..6844,6845..7636)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      7807..8395
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8296..8315
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8549..8566
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8683..8704
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8719..8724,8725..8742,8743..8749)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8757..8773
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8762..8784
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8781..8797
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8781..8797
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8816..8836
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8818..8836
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8864..9090
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             9091..9271
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9318..9443
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9936..10169
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10354..10398
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 gggatattca ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc
       61 cgaaggaata gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa
      121 cggatctcga cggtatcggt taacttttaa aagaaaaggg gggattgggg ggtacagtgc
      181 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
      241 aattacaaaa ttcaaaattt tatcgtccag tgtggtggaa ttctgcagat atcacaactt
      301 tgtatagaaa agttggcctc cgcgccgggt tttggcgcct cccgcgggcg cccccctcct
      361 cacggcgagc gctgccacgt cagacgaagg gcgcagcgag cgtcctgatc cttccgcccg
      421 gacgctcagg acagcggccc gctgctcata agactcggcc ttagaacccc agtatcagca
      481 gaaggacatt ttaggacggg acttgggtga ctctagggca ctggttttct ttccagagag
      541 cggaacaggc gaggaaaagt agtcccttct cggcgattct gcggagggat ctccgtgggg
      601 cggtgaacgc cgatgattat ataaggacgc gccgggtgtg gcacagctag ttccgtcgca
      661 gccgggattt gggtcgcggt tcttgtttgt ggatcgctgt gatcgtcact tggtgagtag
      721 cgggctgctg ggctggccgg ggctttcgtg gccgccgggc cgctcggtgg gacggaagcg
      781 tgtggagaga ccgccaaggg ctgtagtctg ggtccgcgag caaggttgcc ctgaactggg
      841 ggttgggggg agcgcagcaa aatggcggct gttcccgagt cttgaatgga agacgcttgt
      901 gaggcgggct gtgaggtcgt tgaaacaagg tggggggcat ggtgggcggc aagaacccaa
      961 ggtcttgagg ccttcgctaa tgcgggaaag ctcttattcg ggtgagatgg gctggggcac
     1021 catctgggga ccctgacgtg aagtttgtca ctgactggag aactcggttt gtcgtctgtt
     1081 gcgggggcgg cagttatggc ggtgccgttg ggcagtgcac ccgtaccttt gggagcgcgc
     1141 gccctcgtcg tgtcgtgacg tcacccgttc tgttggctta taatgcaggg tggggccacc
     1201 tgccggtagg tgtgcggtag gcttttctcc gtcgcaggac gcagggttcg ggcctagggt
     1261 aggctctcct gaatcgacag gcgccggacc tctggtgagg ggagggataa gtgaggcgtc
     1321 agtttctttg gtcggtttta tgtacctatc ttcttaagta gctgaagctc cggttttgaa
     1381 ctatgcgctc ggggttggcg agtgtgtttt gtgaagtttt ttaggcacct tttgaaatgt
     1441 aatcatttgg gtcaatatgt aattttcagt gttagactag taaattgtcc gctaaattct
     1501 ggccgttttt ggcttttttg ttagacggcc aacttttgta tacaaagtgg gccaccatgg
     1561 gaatcccgct gctgaacgct aaacagattc tgggacagct ggacggcggg agcgtggcag
     1621 tcctgcctgt ggtcgactcc accaatcagt acctgctgga tcgaatcggc gagctgaaga
     1681 gtggggatgc ttgcattgca gaatatcagc aggcagggag aggaagcaga gggaggaaat
     1741 ggttctctcc ttttggagct aacctgtacc tgagtatgtt ttggcgcctg aagcggggac
     1801 cagcagcaat cggcctgggc ccggtcatcg gaattgtcat ggcagaagcg ctgcgaaagc
     1861 tgggagcaga caaggtgcga gtcaaatggc ccaatgacct gtatctgcag gatagaaagc
     1921 tggcaggcat cctggtggag ctggccggaa taacaggcga tgctgcacag atcgtcattg
     1981 gcgccgggat taacgtggct atgaggcgcg tggaggaaag cgtggtcaat cagggctgga
     2041 tcacactgca ggaagcaggg attaacctgg acaggaatac tctggccgct atgctgatcc
     2101 gagagctgcg ggcagccctg gaactgttcg agcaggaagg cctggctcca tatctgtcac
     2161 ggtgggagaa gctggataac ttcatcaata gacccgtgaa gctgatcatt ggggacaaag
     2221 agattttcgg gattagccgg gggattgata aacagggagc cctgctgctg gaacaggacg
     2281 gagttatcaa accctggatg ggcggagaaa tcagtctgcg gtctgccgaa aaggctagcg
     2341 gcaagcccat ccccaacccc ctgctgggcc tggacagcac cacaagtttg tacaaaaaag
     2401 caggctccac catgggcaac gcccccgcca agaaggacac cgagcaggag gagagcgtga
     2461 acgagttcct agccaaagcc agaggagatt tcctctacag atggggaaac cccgctcaaa
     2521 acaccgccag ctcggatcag ttcgaacggc tcaggacgct gggcatgggc tccttcgggc
     2581 gggtgatgct ggtgaggcac caggagaccg gcggccacta cgccatgaag atcctcaaca
     2641 agcagaaggt ggtgaagatg aagcaggtcg agcacatact gaacgagaag cgcatcctgc
     2701 aggcgatcga ctttccgttc ctcgtcaagc tccagttctc ctttaaggac aactcctacc
     2761 tgtacctggt gatggagtac gtgccgggtg gggagatgtt ctcccgccta cagcgcgtcg
     2821 gaaggtttag cgagccccat gcctgtttct atgccgccca ggtcgtcctg gccgtccagt
     2881 acctacactc gctcgacctc atccaccgcg acctgaagcc cgagaatctc ctcatcgacc
     2941 agcagggcta cctgcaggtg acggacttcg gtttcgccaa gcgcgtgaag ggccgcactt
     3001 ggaccttgtg cgggacccca gagtacctgg cccccgagat catcctgagc aaaggctaca
     3061 acaaggccgt ggactggtgg gccctagggg tgctcatcta tgagatggcc gtgggcttcc
     3121 cacccttcta cgccgaccag cccatccaga tctacgagaa gatcgtctct gggagggtgc
     3181 ggtttccctc caaactcagc tctgacctca agcatctgct gcggagcctg ctgcaggtgg
     3241 acctcaccaa gcgcttcgga aacctcagga acggggttgg cgacatcaag aaccacaagt
     3301 ggttcgccac aaccagctgg atcgccatct atgagaagaa ggtggaagct cccttcatcc
     3361 cgaagtacac aggccctggg gatgccagta actttgacga ctacgaggag gaagagctcc
     3421 ggatctccat caatgagaag tgtcccaagg agttttctga gttttaggac ccagctttct
     3481 tgtacaaagt ggtgatatcc agcacagtgg cggccgctcg acaatcaacc tctggattac
     3541 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
     3601 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
     3661 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa
     3721 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc
     3781 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc
     3841 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
     3901 gtggtgttgt cggggaagct gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg
     3961 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct
     4021 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg
     4081 agtcggatct ccctttgggc cgcctccccg cctggaattc taccgggtag gggaggcgct
     4141 tttcccaagg cagtctggag catgcgcttt agcagccccg ctgggcactt ggcgctacac
     4201 aagtggcctc tggcctcgca cacattccac atccaccggt aggcgccaac cggctccgtt
     4261 ctttggtggc cccttcgcgc caccttctac tcctccccta gtcaggaagt tcccccccgc
     4321 cccgcagctc gcgtcgtgca ggacgtgaca aatggaagta gcacgtctca ctagtctcgt
     4381 gcagatggac agcaccgctg agcaatggaa gcgggtaggc ctttggggca gcggccaata
     4441 gcagctttgc tccttcgctt tctgggctca gaggctggga aggggtgggt ccgggggcgg
     4501 gctcaggggc gggctcaggg gcggggcggg cgcccgaagg tcctccggag gcccggcatt
     4561 ctgcacgctt caaaagcgca cgtctgccgc gctgttctcc tcttcctcat ctccgggcct
     4621 ttcgacctgc agcccaagct taccatgacc gagtacaagc ccacggtgcg cctcgccacc
     4681 cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc
     4741 acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct gcaagaactc
     4801 ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg
     4861 gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc
     4921 ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc
     4981 ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc
     5041 gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag
     5101 cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag
     5161 cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc
     5221 atgacccgca agcccggtgc ctgacgcccg ccccacgacc cgcagcgccc gaccgaaagg
     5281 agcgcacgac cccatgcatc tcgagggccc ggtaccttta agaccaatga cttacaaggc
     5341 agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc tagctcactc
     5401 ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag accagatctg
     5461 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
     5521 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
     5581 cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat
     5641 tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact tgtttattgc
     5701 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     5761 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggct
     5821 ctagctatcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     5881 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
     5941 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctagggac gtacccaatt
     6001 cgccctatag tgagtcgtat tacgcgcgct cactggccgt cgttttacaa cgtcgtgact
     6061 gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct
     6121 ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg
     6181 gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
     6241 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
     6301 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
     6361 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac
     6421 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
     6481 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt
     6541 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
     6601 aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttaggtg gcacttttcg
     6661 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     6721 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
     6781 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
     6841 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
     6901 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
     6961 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
     7021 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
     7081 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
     7141 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
     7201 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
     7261 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
     7321 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
     7381 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     7441 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
     7501 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     7561 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     7621 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     7681 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     7741 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     7801 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     7861 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     7921 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
     7981 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     8041 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     8101 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     8161 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     8221 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     8281 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     8341 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     8401 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     8461 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     8521 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
     8581 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga
     8641 caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac
     8701 tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt
     8761 gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca agcgcgcaat
     8821 taaccctcac taaagggaac aaaagctgga gctgcaagct taatgtagtc ttatgcaata
     8881 ctcttgtagt cttgcaacat ggtaacgatg agttagcaac atgccttaca aggagagaaa
     8941 aagcaccgtg catgccgatt ggtggaagta aggtggtacg atcgtgcctt attaggaagg
     9001 caacagacgg gtctgacatg gattggacga accactgaat tgccgcattg cagagatatt
     9061 gtatttaagt gcctagctcg atacataaac gggtctctct ggttagacca gatctgagcc
     9121 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
     9181 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
     9241 cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac ttgaaagcga
     9301 aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa
     9361 gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga ggctagaagg
     9421 agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg cgatgggaaa
     9481 aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata gtatgggcaa
     9541 gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca gaaggctgta
     9601 gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa cttagatcat
     9661 tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata aaagacacca
     9721 aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc gcacagcaag
     9781 cggccgctga tcttcagacc tggaggagga gatatgaggg acaattggag aagtgaatta
     9841 tataaatata aagtagtaaa aattgaacca ttaggagtag cacccaccaa ggcaaagaga
     9901 agagtggtgc agagagaaaa aagagcagtg ggaataggag ctttgttcct tgggttcttg
     9961 ggagcagcag gaagcactat gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa
    10021 ttattgtctg gtatagtgca gcagcagaac aatttgctga gggctattga ggcgcaacag
    10081 catctgttgc aactcacagt ctggggcatc aagcagctcc aggcaagaat cctggctgtg
    10141 gaaagatacc taaaggatca acagctcctg gggatttggg gttgctctgg aaaactcatt
    10201 tgcaccactg ctgtgccttg gaatgctagt tggagtaata aatctctgga acagatttgg
    10261 aatcacacga cctggatgga gtgggacaga gaaattaaca attacacaag cttaatacac
    10321 tccttaattg aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt attggaatta
    10381 gataaatggg caagtttgtg gaattggttt aacataacaa attggctgtg gtatataaaa
    10441 ttattcataa tgatagtagg aggcttggta ggtttaagaa tagtttttgc tgtactttct
    10501 atagtgaata gagttaggca
//
