LOCUS       sequence-335771-       11690 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway destination clone of RNASEL (human) tagged with N-terminal 
            miniTurbo-V5 for generating protein-proximity networks using 
            proximity-dependent biotinylation proteomics.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11690)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 11690)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p663-UBC-miniTurbo-V5-RNASEL_IDG-K
FEATURES             Location/Qualifiers
     source          1..11690
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             81..680
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(81..100)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     577..597
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             818..1051
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    1123..1244
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1160..1179)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1214..1233
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      1284..1419
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(1439..1458)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1440..1458)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1468..1485)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1468..1484)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1477..1499)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1626..2081
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1713..1732)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1923..1944
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2107..2211
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2212..2280,2281..3072)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      3243..3831
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3732..3751
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3985..4002
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4119..4140
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4155..4160,4161..4178,4179..4185)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4193..4209
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4198..4220
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4217..4233
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4217..4233
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     4252..4272
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        4254..4272
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        4300..4526
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             4527..4707
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4754..4879
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5372..5605
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5790..5834
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6101..6217
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    6251..6271
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        6271..7482
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     7319..7338
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    complement(7486..7506)
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     regulatory      7507..7516
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7519..8289
                     /codon_start=1
                     /product="N-terminally truncated mutant of the E. coli 
                     biotin ligase BioID engineered for improved catalytic 
                     efficiency (Branon et al., 2018)"
                     /label=miniTurbo
                     /translation="IPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGDACI
                     AEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLGADK
                     VRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWITLQ
                     EAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIF
                     GISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     CDS             8296..8337
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    8338..8362
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(10595..10619)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    10649..11237
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(10702..10722)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(11120..11131)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        join(11251..11690,1..60)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(11286..11309)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     join(11683..11690,1..12)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
ORIGIN
        1 acgcttcaaa agcgcacgtc tgccgcgctg ttctcctctt cctcatctcc gggcctttcg
       61 acctgcagcc caagcttacc atgaccgagt acaagcccac ggtgcgcctc gccacccgcg
      121 acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc
      181 gccacaccgt cgatccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc
      241 tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg
      301 cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc
      361 gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg
      421 cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc
      481 accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg
      541 ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc
      601 tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga
      661 cccgcaagcc cggtgcctga cgcccgcccc acgacccgca gcgcccgacc gaaaggagcg
      721 cacgacccca tgcatctcga gggcccggta cctttaagac caatgactta caaggcagct
      781 gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctagc tcactcccaa
      841 cgaagacaag atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc
      901 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
      961 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
     1021 cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag
     1081 tatttataac ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt tattgcagct
     1141 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     1201 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggctctag
     1261 ctatcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
     1321 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg
     1381 agctattcca gaagtagtga ggaggctttt ttggaggcct agggacgtac ccaattcgcc
     1441 ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga
     1501 aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg
     1561 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga
     1621 atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
     1681 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
     1741 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
     1801 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
     1861 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
     1921 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
     1981 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
     2041 atttaacgcg aattttaaca aaatattaac gcttacaatt taggtggcac ttttcgggga
     2101 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     2161 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     2221 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     2281 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     2341 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     2401 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
     2461 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     2521 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     2581 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     2641 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     2701 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
     2761 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     2821 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     2881 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     2941 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     3001 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     3061 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     3121 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     3181 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     3241 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     3301 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     3361 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     3421 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     3481 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     3541 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     3601 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     3661 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     3721 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     3781 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     3841 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     3901 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     3961 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca
     4021 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg
     4081 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat
     4141 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc
     4201 ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagcg cgcaattaac
     4261 cctcactaaa gggaacaaaa gctggagctg caagcttaat gtagtcttat gcaatactct
     4321 tgtagtcttg caacatggta acgatgagtt agcaacatgc cttacaagga gagaaaaagc
     4381 accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg tgccttatta ggaaggcaac
     4441 agacgggtct gacatggatt ggacgaacca ctgaattgcc gcattgcaga gatattgtat
     4501 ttaagtgcct agctcgatac ataaacgggt ctctctggtt agaccagatc tgagcctggg
     4561 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
     4621 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
     4681 tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga aagcgaaagg
     4741 gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca cggcaagagg
     4801 cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct agaaggagag
     4861 agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat gggaaaaaat
     4921 tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat gggcaagcag
     4981 ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag gctgtagaca
     5041 aatactggga cagctacaac catcccttca gacaggatca gaagaactta gatcattata
     5101 taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag acaccaagga
     5161 agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac agcaagcggc
     5221 cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt gaattatata
     5281 aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag
     5341 tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag
     5401 cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc agacaattat
     5461 tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc
     5521 tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa
     5581 gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca
     5641 ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag atttggaatc
     5701 acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta atacactcct
     5761 taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg gaattagata
     5821 aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat ataaaattat
     5881 tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag
     5941 tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga
     6001 ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac agagacagat
     6061 ccattcgatt agtgaacgga tctcgacggt atcggttaac ttttaaaaga aaagggggga
     6121 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
     6181 aagaattaca aaaacaaatt acaaaattca aaattttatc gtccagtgtg gtggaattct
     6241 gcagatatca caactttgta tagaaaagtt ggcctccgcg ccgggttttg gcgcctcccg
     6301 cgggcgcccc cctcctcacg gcgagcgctg ccacgtcaga cgaagggcgc agcgagcgtc
     6361 ctgatccttc cgcccggacg ctcaggacag cggcccgctg ctcataagac tcggccttag
     6421 aaccccagta tcagcagaag gacattttag gacgggactt gggtgactct agggcactgg
     6481 ttttctttcc agagagcgga acaggcgagg aaaagtagtc ccttctcggc gattctgcgg
     6541 agggatctcc gtggggcggt gaacgccgat gattatataa ggacgcgccg ggtgtggcac
     6601 agctagttcc gtcgcagccg ggatttgggt cgcggttctt gtttgtggat cgctgtgatc
     6661 gtcacttggt gagtagcggg ctgctgggct ggccggggct ttcgtggccg ccgggccgct
     6721 cggtgggacg gaagcgtgtg gagagaccgc caagggctgt agtctgggtc cgcgagcaag
     6781 gttgccctga actgggggtt ggggggagcg cagcaaaatg gcggctgttc ccgagtcttg
     6841 aatggaagac gcttgtgagg cgggctgtga ggtcgttgaa acaaggtggg gggcatggtg
     6901 ggcggcaaga acccaaggtc ttgaggcctt cgctaatgcg ggaaagctct tattcgggtg
     6961 agatgggctg gggcaccatc tggggaccct gacgtgaagt ttgtcactga ctggagaact
     7021 cggtttgtcg tctgttgcgg gggcggcagt tatggcggtg ccgttgggca gtgcacccgt
     7081 acctttggga gcgcgcgccc tcgtcgtgtc gtgacgtcac ccgttctgtt ggcttataat
     7141 gcagggtggg gccacctgcc ggtaggtgtg cggtaggctt ttctccgtcg caggacgcag
     7201 ggttcgggcc tagggtaggc tctcctgaat cgacaggcgc cggacctctg gtgaggggag
     7261 ggataagtga ggcgtcagtt tctttggtcg gttttatgta cctatcttct taagtagctg
     7321 aagctccggt tttgaactat gcgctcgggg ttggcgagtg tgttttgtga agttttttag
     7381 gcaccttttg aaatgtaatc atttgggtca atatgtaatt ttcagtgtta gactagtaaa
     7441 ttgtccgcta aattctggcc gtttttggct tttttgttag acggccaact tttgtataca
     7501 aagtgggcca ccatgggaat cccgctgctg aacgctaaac agattctggg acagctggac
     7561 ggcgggagcg tggcagtcct gcctgtggtc gactccacca atcagtacct gctggatcga
     7621 atcggcgagc tgaagagtgg ggatgcttgc attgcagaat atcagcaggc agggagagga
     7681 agcagaggga ggaaatggtt ctctcctttt ggagctaacc tgtacctgag tatgttttgg
     7741 cgcctgaagc ggggaccagc agcaatcggc ctgggcccgg tcatcggaat tgtcatggca
     7801 gaagcgctgc gaaagctggg agcagacaag gtgcgagtca aatggcccaa tgacctgtat
     7861 ctgcaggata gaaagctggc aggcatcctg gtggagctgg ccggaataac aggcgatgct
     7921 gcacagatcg tcattggcgc cgggattaac gtggctatga ggcgcgtgga ggaaagcgtg
     7981 gtcaatcagg gctggatcac actgcaggaa gcagggatta acctggacag gaatactctg
     8041 gccgctatgc tgatccgaga gctgcgggca gccctggaac tgttcgagca ggaaggcctg
     8101 gctccatatc tgtcacggtg ggagaagctg gataacttca tcaatagacc cgtgaagctg
     8161 atcattgggg acaaagagat tttcgggatt agccggggga ttgataaaca gggagccctg
     8221 ctgctggaac aggacggagt tatcaaaccc tggatgggcg gagaaatcag tctgcggtct
     8281 gccgaaaagg ctagcggcaa gcccatcccc aaccccctgc tgggcctgga cagcaccaca
     8341 agtttgtaca aaaaagcagg ctccaccatg gagagcaggg atcataacaa cccccaggag
     8401 ggacccacgt cctccagcgg tagaagggct gcagtggaag acaatcactt gctgattaaa
     8461 gctgttcaaa acgaagatgt tgacctggtc cagcaattgc tggaaggtgg agccaatgtt
     8521 aatttccagg aagaggaagg gggctggaca cctctgcata acgcagtaca aatgagcagg
     8581 gaggacattg tggaacttct gcttcgtcat ggtgctgacc ctgttctgag gaagaagaat
     8641 ggggccacgc cttttatcct cgcagcgatt gcggggagcg tgaagctgct gaaacttttc
     8701 ctttctaaag gagcagatgt caatgagtgt gatttttatg gcttcacagc cttcatggaa
     8761 gccgctgtgt atggtaaggt caaagcccta aaattccttt ataagagagg agcaaatgtg
     8821 aatttgaggc gaaagacaaa ggaggatcaa gagcggctga ggaaaggagg ggccacagct
     8881 ctcatggacg ctgctgaaaa aggacacgta gaggtcttga agattctcct tgatgagatg
     8941 ggggcagatg taaacgcctg tgacaatatg ggcagaaatg ccttgatcca tgctctcctg
     9001 agctctgacg atagtgatgt ggaggctatt acgcatctgc tgctggacca tggggctgat
     9061 gtcaatgtga ggggagaaag agggaagact cccctgatcc tggcagtgga gaagaagcac
     9121 ttgggtttgg tgcagaggct tctggagcaa gagcacatag agattaatga cacagacagt
     9181 gatggcaaaa cagcactgct gcttgctgtt gaactcaaac tgaagaaaat cgccgagttg
     9241 ctgtgcaaac gtggagccag tacagattgt ggggatcttg ttatgacagc gaggcggaat
     9301 tatgaccatt cccttgtgaa ggttcttctc tctcatggag ccaaagaaga ttttcaccct
     9361 cctgctgaag actggaagcc tcagagctca cactgggggg cagccctgaa ggatctccac
     9421 agaatatacc gccctatgat tggcaaactc aagttcttta ttgatgaaaa atacaaaatt
     9481 gctgatactt cagaaggagg catctacctg gggttctatg agaagcaaga agtagctgtg
     9541 aagacgttct gtgagggcag cccacgtgca cagcgggaag tctcttgtct gcaaagcagc
     9601 cgagagaaca gtcacttggt gacattctat gggagtgaga gccacagggg ccacttgttt
     9661 gtgtgtgtca ccctctgtga gcagactctg gaagcgtgtt tggatgtgca cagaggggaa
     9721 gatgtggaaa atgaggaaga tgaatttgcc cgaaatgtcc tgtcatctat atttaaggct
     9781 gttcaagaac tacacttgtc ctgtggatac acccaccagg atctgcaacc acaaaacatc
     9841 ttaatagatt ctaagaaagc tgctcacctg gcagattttg ataagagcat caagtgggct
     9901 ggagatccac aggaagtcaa gagagatcta gaggaccttg gacggctggt cctctatgtg
     9961 gtaaagaagg gaagcatctc atttgaggag ctgaaagctc aaagtaatga agaggtggtt
    10021 caactttctc cagatgagga aactaaggac ctcattcatc gtctcttcca tcctggggaa
    10081 catgtgaggg actgtctgag tgacctgctg ggtcatccct tcttttggac ttgggagagc
    10141 cgctatagga cgcttcggaa tgtgggaaat gaatccgaca tcaaaacacg aaaatctgaa
    10201 agtgagatcc tcagactact gcaacctggg ccttctgaac attccaaaag ttttgacaag
    10261 tggacgacta agattaatga atgtgttatg aaaaaaatga ataagtttta tgaaaaaaga
    10321 ggcaatttct accagaacac tgtgggtgat ctgctaaagt tcatccggaa tttgggagaa
    10381 cacattgatg aagaaaagca taaaaagatg aaattaaaaa ttggagaccc ttccctgtat
    10441 tttcagaaga catttccaga tctggtgatc tatgtctaca caaaactaca gaacacagaa
    10501 tatagaaagc atttccccca aacccacagt ccaaacaagt ctcagtgtga tggagctggt
    10561 ggggccagtg ggttggccag ccctgggtgc taggacccag ctttcttgta caaagtggtg
    10621 atatccagca cagtggcggc cgctcgacaa tcaacctctg gattacaaaa tttgtgaaag
    10681 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat
    10741 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc
    10801 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg
    10861 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct
    10921 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct
    10981 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg
    11041 gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac
    11101 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct
    11161 gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct
    11221 ttgggccgcc tccccgcctg gaattctacc gggtagggga ggcgcttttc ccaaggcagt
    11281 ctggagcatg cgctttagca gccccgctgg gcacttggcg ctacacaagt ggcctctggc
    11341 ctcgcacaca ttccacatcc accggtaggc gccaaccggc tccgttcttt ggtggcccct
    11401 tcgcgccacc ttctactcct cccctagtca ggaagttccc ccccgccccg cagctcgcgt
    11461 cgtgcaggac gtgacaaatg gaagtagcac gtctcactag tctcgtgcag atggacagca
    11521 ccgctgagca atggaagcgg gtaggccttt ggggcagcgg ccaatagcag ctttgctcct
    11581 tcgctttctg ggctcagagg ctgggaaggg gtgggtccgg gggcgggctc aggggcgggc
    11641 tcaggggcgg ggcgggcgcc cgaaggtcct ccggaggccc ggcattctgc
//
