LOCUS       sequence-335772-        1135 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Streptomyces codon-optimised beta-D-glucuronidase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1135)
  AUTHORS   Moore SJ, Lai HE, Chee SM, Toh M, Coode S, Chengan K, Capel P, Corre
            C, de Los Santos EL, Freemont PS
  TITLE     A Streptomyces venezuelae Cell-Free Toolkit for Synthetic Biology.
  JOURNAL   ACS Synth Biol. 2021 Feb 19;10(2):402-411. doi: 
            10.1021/acssynbio.0c00581. Epub 2021 Jan 26.
  PUBMED    33497199
REFERENCE   2  (bases 1 to 1135)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.0c00581"; journalName: "ACS Synth Biol"; date: 
            "2021-02-19- 19"; volume: "10"; issue: "2"; pages: "402-411"
COMMENT     Sequence Label: pTU1-B-SP44s-GUS
FEATURES             Location/Qualifiers
     source          1..1135
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     19..36
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 caccgaacga ccgagcgcag cgagtcagtg agcgaggaag cctgcagcgt ctctccggaa
       61 cattttgtta tcaataaaaa aggccccccg atttgggagg cctttttttt tttttcgagg
      121 atcctcactg cttgccgccc tgctgcggct tctcgccgaa gttcatgccg gtccagcgct
      181 tctggaggag gaaggcggcg gacttcggct tccggtcgcg ggtgaagatg cccttcttgt
      241 tgccgccgac gcgcaggatg ccctgcgagg tggcgaagtc ggcgaagttc cagacctgct
      301 cgccgacgac ggcgctgacg cggtcgaaca cgcggtggta catgtcgagc caggcgcact
      361 ggtactcctc ggaccacatg tcggtgtaca tggagtggag gcccgcgagg gtgtcgacgc
      421 cgtactcggt gatgatgatc ggctggtgca gcttctcctg ccaggccagc agctccttct
      481 cgaggacctt ctccgcggtt tcgaggtcgc cggactggac gtaccagccg tagtagcggt
      541 tgaggcagag gacgtcgaag aggtcggaga tggtgtcggt gtgggcgtcg cagaacatga
      601 cgttgacgca cgtgatcggc cgcgtcgggt ccagcttgcg ggtcgcctcg gcgagcgggg
      661 cgaagtactc gcgggcgccc tgcgggcgcg tgtccggctc gttcgcgatc gaccacatga
      721 cgacggacgg gtggttcttg tcgcgggcga tcagctcctt gatggcctgg aggtgggcct
      781 gctgggtttc gccgttgacc gcctcctcgg agtacagctc cttcggcttg ttgcccgcct
      841 cgaagccgat gccgagcgag aggttgaagc cgacggcggc ggtttcgtcg atgacgacga
      901 tgccgtgctc gtcggcccag tcgagcatct cctcggcgta cgggtagtgc gaggtgcggt
      961 aggagttggc gccgatccag tccatgagcg cgtggtcgtg gaccatgagg acgttgtcga
     1021 agcccttgcc gcggaggtcc gcgtcctcgt ggcggccgaa gccggtgaag taaaacggct
     1081 tgtggttgaa gaggaactgc tcgcccttga cggggacgaa cggatgccaa cccga
//
