LOCUS       sequence-33578-e         857 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 857)
  AUTHORS   Rothenfusser S, Goutagny N, DiPerna G, Gong M, Monks BG, 
            Schoenemeyer A, Yamamoto M, Akira S, Fitzgerald KA
  TITLE     The RNA helicase Lgp2 inhibits TLR-independent sensing of viral 
            replication by retinoic acid-inducible gene-I.
  JOURNAL   J Immunol. 2005 Oct 15. 175(8):5260-8.
  PUBMED    16210631
REFERENCE   2  (bases 1 to 857)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Immunol. 
            2005 Oct 15. 175(8):5260-8."
COMMENT     Sequence Label: pEF-BOS RIG-I
FEATURES             Location/Qualifiers
     source          1..857
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(59..76)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(77..100)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(110..121)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 acctgatact ctcaagggtc ccaggtgggg accctcactc tagagtcgcg gccgctcagt
       61 ggtggtggtg gtggtgcttg tcatcgtcgt ccttgtagtc cttgacgatc cttccttcaa
      121 tggatccttt ggacatttct gctggatcaa atggtatctt ctcaaaatga aagtccttcc
      181 acttcgagta cagcgtctga actccagttg caatatcctc caccacaaaa ctttcaattt
      241 ttataactgg aatctcaaat gtcttgtact tcacatggat tccccagtca tggctgcagt
      301 tctgtcgggc acagaatatc tttgctcttt tttcaaaact tgaaaactgc tttggcttgg
      361 gatgtggtct actcacaaag cattccttaa aagcatctcc aagcacagtg taatggcatt
      421 cctctatcac tcttacgtca gctgtgtaac atgccaaggc tttgcacttt ctgcagagca
      481 gttttttatt ttccttatca ggtacaggtt ttggtttttc ttgactatct ctgatgaatt
      541 tttcatgagt ctgtatatgc agaatctttt ccctaaatac tgcttcgtcc catgtctgaa
      601 ggcgtaaaat agagtcattc atcatttttt ctttgtacat gtttatttgt tctttttcaa
      661 ttacaccagc attactagtc agaaggaagc acttgctacc tcttgctctt cctctgcctc
      721 tggtttggat cattttgatg acattgccca catactcata aaggatgaca agattgcact
      781 gtgcaatgtc aatgccttca tcagcaactg aggtggcaat cagaatattg tgatctccac
      841 tggctttgaa tgcatcc
//
