LOCUS       sequence-335781-        1471 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Ism1 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1471)
  AUTHORS   Jiang Z, Zhao M, Voilquin L, Jung Y, Aikio MA, Sahai T, Dou FY, 
            Roche AM, Carcamo-Orive I, Knowles JW, Wabitsch M, Appel EA, Maikawa
            CL, Camporez JP, Shulman GI, Tsai L, Rosen ED, Gardner CD, 
            Spiegelman BM, Svensson KJ
  TITLE     Isthmin-1 is an adipokine that promotes glucose uptake and improves 
            glucose tolerance and hepatic steatosis.
  JOURNAL   Cell Metab. 2021 Jul 28. pii: S1550-4131(21)00326-0. doi: 
            10.1016/j.cmet.2021.07.010.
  PUBMED    34348115
REFERENCE   2  (bases 1 to 1471)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Metab.
            2021 Jul 28. pii: S1550-4131(21)00326-0. doi: 
            10.1016/j.cmet.2021.07.010."
COMMENT     Sequence Label: Mouse Ism1 with C-terminal Myc-6X-his tag
FEATURES             Location/Qualifiers
     source          1..1471
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1402..1431
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1450..1467
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atggtgcgcc tggctgcgga actgctcctg ctgctggggc tactgctgct cacgctgcac
       61 atcacggttc tgcgcggatc cggagcctcc gaccggcagg acgcggccgc cggcaacgtc
      121 agcgggtccc agttgcagaa taacctcaac ctggagagtg actccacgtc agaaaccagc
      181 tttcctctgt ccaaagaagc cccggaggag caccaggttg tccaccaacc cttccccaga
      241 cagcgattcc ctccagagac tggacatcct tcactgcaga gagacggccc cagatccttt
      301 ctccttgacc taccaaactt tccagatctt tccaaagctg atatcaatgg gcagaatcca
      361 aacattcagg tcaccataga ggtggttgat ggccctgact ccgaagcaga aaaagatcag
      421 catccagaga ataaacccag ctggtcgctc ccagctcccg actggcgggc ctggtggcag
      481 aggtccttgt ccttggccag gacaaatagt ggggaccagg atgacaagta tgacagtacc
      541 tcagatgaca gcaacttcct cagtgttcct agaggatggg accgtccagc cccaggacac
      601 cggacttttg aaaccaaaga gcagccagag tatgattcca cagacgggga gggtgattgg
      661 agtctctggt ctgtctgcag cgtcacctgt ggaaatggca accagaaacg gacccgttct
      721 tgtggctatg catgcattgc cacagaatcc aggacctgtg accgtccaaa ctgcccagga
      781 attgaagata ctttcaggac agcggccact gaagtgagtc tgcttgcggg aagtgaggag
      841 tttaatgcca ccaagttgtt tgaagttgac atggacagct gtgagcgatg gatgagctgc
      901 aaaagtgagt tcttaaagaa atacatgcac aaggtgatca acgacctgcc cagctgcccc
      961 tgctcctacc ctactgaggt ggcctacagc acagctgaca tctttgaccg catcaagcgc
     1021 aaggacttcc gatggaagga tgctagtggg cccaaagaga aactagagat ctacaagcct
     1081 actgctcggt actgcatccg ctctatgctg tccctggaga gtaccacact ggctgcccag
     1141 cactgttgct atggtgacaa catgcagctc atcaccaggg gcaaaggggc aggcactccc
     1201 aatctcatca gcaccgagtt ctctgctgaa ctccactaca aagtggatgt tctgccctgg
     1261 attatctgca agggtgactg gagcagatat aatgaggccc ggcctcccaa taacggacag
     1321 aagtgcacag agagcccttc tgatgaggac tacatcaaac agttccaaga agccagagag
     1381 tacacgcgta cgcggccgct cgagcagaaa ctcatctcag aagaggatct ggcagcaaat
     1441 gatatcctgc accaccatca ccatcacgtt t
//
