LOCUS       sequence-33579-e         840 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 840)
  AUTHORS   Hornung V, Ablasser A, Charrel-Dennis M, Bauernfeind F, Horvath G, 
            Caffrey DR, Latz E, Fitzgerald KA
  TITLE     AIM2 recognizes cytosolic dsDNA and forms a caspase-1-activating 
            inflammasome with ASC.
  JOURNAL   Nature. 2009 Mar 26. 458(7237):514-8.
  PUBMED    19158675
REFERENCE   2  (bases 1 to 840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2009 Mar 26. 458(7237):514-8."
COMMENT     Sequence Label: pEF-BOS AIM2
FEATURES             Location/Qualifiers
     source          1..840
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(51..68)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(69..92)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(102..113)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 ctctcaaggg tcccaggtgg ggaccctcac tctagagtcg cggccgctca gtggtggtgg
       61 tggtggtgct tgtcatcgtc gtccttgtag tccttgacga tccttccttc aatggatccg
      121 accagttggc ttgaattggt cctttttact tctctatgtt tttttttttt ggccttaata
      181 acctttatgg tgctatgaac tccagatgtc agctgtagtt tttctccatt ttttgacagt
      241 gtgaagaatg taagtcgaac cttatctcct tccttacatt tcattgtgtc ctcgtttcta
      301 acccccagta cttccatttt cccagtgttg tcacttaggt caaataatat gtttttcttc
      361 ttttctgtta ccttctggac tacaaacaaa ccattcacaa ttgttccaag gggctgagtt
      421 tgaagcgtgt tgatcttcgg ggtttcacca gcttttctga taatgttcag cgggacatta
      481 accttttggt cagattcagc atctaacaca cgtgaggcgc tatttacctc taagaaacca
      541 ctgtgccgat aatatcttgc tattataatt attctctttg gaatgaattt atctttcagc
      601 agtgtattaa aaacttttac aaagaagaat tccttttctg tagccactgt agcatgaaac
      661 atctcctgct tgccttcttg ggtctcaaac gtgaagggct tctttgcttt cagtaccata
      721 actggcaaac agcgcttctg aaacccttct ctgatagatt cctgctgggg ccaccatctg
      781 tttctgttca ggcttaacat gaggagagac ttttggtgca gcacgttgct ttgcgacatc
//
