LOCUS       sequence-335790-        8728 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  HiLITR protease with AKAP1 N-terminal tmd targeting information 
            (Mitochondria).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8728)
  AUTHORS   Coukos RW, Yao D, Sanchez ML, Strand ET, Olive ME, Udeshi ND, 
            Weissman JS, Carr SA, Bassik MC, Ting AY
  TITLE     An engineered transcriptional reporter of protein localization 
            identifies regulators of mitochondrial and ER membrane protein 
            trafficking in high-throughput CRISPRi screens.
  JOURNAL   eLife. 2021 Aug 20;10. pii: 69142. doi: 10.7554/eLife.69142.
  PUBMED    34414886
REFERENCE   2  (bases 1 to 8728)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife."; 
            date: "2021-08-20"; volume: "10. pii: 69142. doi"; pages: " 
            10.7554/eLife.69142"
COMMENT     Sequence Label: AKAP1-EGFP-uTEV1
FEATURES             Location/Qualifiers
     source          1..8728
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     35..59
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      79..88
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(274..276,277..279,280..990)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(274..276,277..279,280..990)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(319..340)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(580..599)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     927..948
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        1857..2367
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     2286..2304
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             join(2377..2994,2995..3000,3001..3036,3037..3039,3040..3075,
                     3076..3078,3079..3114,3115..3123)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(3163..3183)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3581..3592)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3797..4030
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4102..4223
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4139..4158)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4193..4212
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4263..4398
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        4398..4469
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4470..4538,4539..5330)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4688..4707)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5501..6089
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5990..6009
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6243..6260
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6377..6398
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6413..6418,6419..6436,6437..6443)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6451..6467
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6456..6478
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6475..6491
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6475..6491
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6510..6530
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6512..6530
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6558..6784
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6785..6965
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7012..7137
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7630..7863
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8048..8092
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8305..8422
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        join(8476..8728,1..62)
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    8484..8502
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8520..8538
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8555..8573
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8591..8609
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8627..8645
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8662..8680
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8698..8716
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 gtaggcgtgt acggtgggag gcctatataa gcagagctcg tttagtgaac cgtcagatcg
       61 cctggagaat tggctagcgc caccatggcc atccagctgc gcagcctgtt ccccctggcc
      121 ctgcccggcc tgctggccct gctgggctgg tggtggttct tcagccgcaa gaaggacctg
      181 gagctgaagc tgcgcatcct gcagagcacc gtgccccgcg cccgcgaccc tcccgtggcc
      241 accggttcca gtggaggcgg tagctcaggt tcgatggtga gcaagggcga ggagctgttc
      301 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc
      361 gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc
      421 accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac ctacggcgtg
      481 cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg
      541 cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc
      601 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc
      661 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac
      721 aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc
      781 cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc
      841 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc cgccctgagc
      901 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg
      961 atcactctcg gcatggacga gctgtacaag gaccttggat ctggcacagg cggatccggt
     1021 tcaggcggag aaagcttgtt taagggacca cgtgattaca acccgatatc gagcaccatt
     1081 tgtcatttga cgaatgaatc tgatgggcac acaacatcgt tgtatggtat tggatttggt
     1141 cccttcatca ttacaaacaa gcacttgttt agaagaaata atggaacact gttggtccaa
     1201 tcactacatg gtgtattcaa ggtcaagaac accacgactt tgcaacaaca cctcattgat
     1261 gggagggaca tgataattat tcgcatgcct aaggatttcc caccatttcc tcaaaagctg
     1321 aaatttagag agccacaaag ggaagagcgc atatgtcttg tgacaaccaa cttccaaact
     1381 aagagcatgt ctagcatggt gtcagacact agttgcacat tcccttcatc tgatggcata
     1441 ttctggaagc attggattca aaccaaggat gggcagtgtg gcaatccatt agtatcaact
     1501 agagatgggt tcattgttgg tatacactca gcatcgaatt tcaccaacac aaacaattat
     1561 ttcacaagcg tgccgaaaaa cttcatggaa ttgttgacaa atcaggaggc gcagcagtgg
     1621 gttagtggtt ggcgattaaa tgctgactca gtattgtggg ggggccataa agttttcatg
     1681 gtggaattcg gttccacgtc aggttctggc aagcccggat caggcgaggg tagcggctct
     1741 acttctgggt ctggtaagcc tgggagtgga gaggggagcg gcagttccgg ctcaggtagt
     1801 ggagagctgg ccgagaagct ggccggcctg gacatcaact aaggccgaat tcatccgggg
     1861 ttggggttgc gccttttcca aggcagccct gggtttgcgc agggacgcgg ctgctctggg
     1921 cgtggttccg ggaaacgcag cggcgccgac cctgggtctc gcacattctt cacgtccgtt
     1981 cgcagcgtca cccggatctt cgccgctacc cttgtgggcc ccccggcgac gcttcctgct
     2041 ccgcccctaa gtcgggaagg ttccttgcgg ttcgcggcgt gccggacgtg acaaacggaa
     2101 gccgcacgtc tcactagtac cctcgcagac ggacagcgcc agggagcaat ggcagcgcgc
     2161 cgaccgcgat gggctgtggc caatagcggc tgctcagcag ggcgcgccga gagcagcggc
     2221 cgggaagggg cggtgcggga ggcggggtgt ggggcggtag tgtgggccct gttcctgccc
     2281 gcgcggtgtt ccgcattctg caagcctccg gagcgcacgt cggcagtcgg ctccctcgtt
     2341 gaccgaatca ccgacctctc tccccagcaa ttcaccatgt ctagactgga caagagcaaa
     2401 gtcataaacg gagctctgga attactcaat ggtgtcggta tcgaaggcct gacgacaagg
     2461 aaactcgctc aaaagctggg agttgagcag cctaccctgt actggcacgt gaagaacaag
     2521 cgggccctgc tcgatgccct gccaatcgag atgctggaca ggcatcatac ccacttctgc
     2581 cccctggaag gcgagtcatg gcaagacttt ctgcggaaca acgccaagtc ataccgctgt
     2641 gctctcctct cacatcgcga cggggctaaa gtgcatctcg gcacccgccc aacagagaaa
     2701 cagtacgaaa ccctggaaaa tcagctcgcg ttcctgtgtc agcaaggctt ctccctggag
     2761 aacgcactgt acgctctgtc cgccgtgggc cactttacac tgggctgcgt attggaggaa
     2821 caggagcatc aagtagcaaa agaggaaaga gagacaccta ccaccgattc tatgccccca
     2881 cttctgagac aagcaattga gctgttcgac cggcagggag ccgaacctgc cttccttttc
     2941 ggcctggaac taatcatatg tggcctggag aaacagctaa agtgcgaaag cggcgggccg
     3001 accgacgccc ttgacgattt tgacttagac atgctcccag ccgatgccct tgacgacttt
     3061 gaccttgata tgctgcctgc tgacgctctt gacgattttg accttgacat gctccccggg
     3121 taactaagta aggatcgatc caagatatcg tattcttaac tatgttgctc cttttacgct
     3181 atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat
     3241 tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt
     3301 caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat
     3361 tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc
     3421 ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga
     3481 caattccgtg gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg cctgtgttgc
     3541 cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga
     3601 ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc
     3661 tcagacgagt cggatctccc tttgggccgc ctccccgcct gtttcgcctc ggcgtccgga
     3721 ctagaggtac ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta
     3781 aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga tctgcttttt
     3841 gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
     3901 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
     3961 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
     4021 atctctagca gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa
     4081 tgaatatcag agagtgagag gaacttgttt attgcagctt ataatggtta caaataaagc
     4141 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     4201 tccaaactca tcaatgtatc ttatcatgtc tggctctagc tatcccgccc ctaactccgc
     4261 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
     4321 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag
     4381 gaggcttttt tggaggcctt tcaaatatgt atccgctcat gagacaataa ccctgataaa
     4441 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     4501 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     4561 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     4621 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     4681 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     4741 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     4801 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     4861 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     4921 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     4981 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     5041 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     5101 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     5161 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     5221 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     5281 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     5341 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     5401 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     5461 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     5521 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     5581 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     5641 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     5701 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     5761 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     5821 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     5881 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     5941 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     6001 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     6061 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     6121 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     6181 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     6241 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
     6301 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
     6361 gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact
     6421 ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa
     6481 acagctatga ccatgattac gccaagcgcg caattaaccc tcactaaagg gaacaaaagc
     6541 tggagctgca agcttaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac
     6601 gatgagttag caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga
     6661 agtaaggtgg tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg
     6721 acgaaccact gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat
     6781 aaacgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
     6841 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
     6901 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
     6961 tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga
     7021 cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt
     7081 acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt
     7141 attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag
     7201 aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt
     7261 aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca
     7321 tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc aaccctctat
     7381 tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa
     7441 gagcaaaaca aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg
     7501 aggagatatg agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga
     7561 accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc
     7621 agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc
     7681 agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca
     7741 gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg
     7801 catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct
     7861 cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc
     7921 tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga
     7981 cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca
     8041 gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg
     8101 gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt
     8161 ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata
     8221 ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacaattct cgacctcgag
     8281 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     8341 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     8401 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcc
     8461 gcgaatcgat atgtcgagtt tactccctat cagtgataga gaacgtatgt cgagtttact
     8521 ccctatcagt gatagagaac gatgtcgagt ttactcccta tcagtgatag agaacgtatg
     8581 tcgagtttac tccctatcag tgatagagaa cgtatgtcga gtttactccc tatcagtgat
     8641 agagaacgta tgtcgagttt atccctatca gtgatagaga acgtatgtcg agtttactcc
     8701 ctatcagtga tagagaacgt atgtcgag
//
