LOCUS       sequence-335799-        8792 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  bacterial expression for purification of PGL-3, allowing cleavage 
            for MBP tag using TEV protease.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8792)
  AUTHORS   Schmidt H, Putnam A, Rasoloson D, Seydoux G
  TITLE     Coordination of RNA and protein condensation by the P granule 
            protein MEG-3
  JOURNAL   bioRxiv 2020.10.15.340570
REFERENCE   2  (bases 1 to 8792)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2020.10.15.340570"
COMMENT     Sequence Label: pAAP2
FEATURES             Location/Qualifiers
     source          1..8792
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..80
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             81..1163
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(100..119)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        join(1406..1411,1412..1427,1428..1434)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    1442..1458
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1447..1469
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     CDS             1528..2628
                     /codon_start=1
                     /gene="malE (mutated)"
                     /product="maltose binding protein from E. coli"
                     /label=MBP
                     /note="This version of the gene does not encode a signal 
                     sequence, so MBP will remain in the cytosol."
                     /translation="MKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLE
                     EKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKL
                     IAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIA
                     ADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGE
                     TAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFL
                     ENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAF
                     WYAVRTAVINAASGRQTVDEALKDAQT"
     primer_bind     2602..2625
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     CDS             2677..2694
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             2695..2715
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     protein_bind    complement(4808..4832)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(4883..4900)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4883..4899)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4892..4914)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(5078..5095)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      5248..5334
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      5426..5453
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        5472..5563
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5564..5632,5633..6424)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5782..5801)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      complement(6466..6979)
                     /direction=LEFT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6661..6682)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6873..6892
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     rep_origin      7090..7678
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7579..7598
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7832..7849
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    7864..8006
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(7932..7951)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8092..8114
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(8108..8299)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
ORIGIN
        1 ccgacaccat cgaatggtgc aaaacctttc gcggtatggc atgatagcgc ccggaagaga
       61 gtcaattcag ggtggtgaat gtgaaaccag taacgttata cgatgtcgca gagtatgccg
      121 gtgtctctta tcagaccgtt tcccgcgtgg tgaaccaggc cagccacgtt tctgcgaaaa
      181 cgcgggaaaa agtggaagcg gcgatggcgg agctgaatta cattcccaac cgcgtggcac
      241 aacaactggc gggcaaacag tcgttgctga ttggcgttgc cacctccagt ctggccctgc
      301 acgcgccgtc gcaaattgtc gcggcgatta aatctcgcgc cgatcaactg ggtgccagcg
      361 tggtggtgtc gatggtagaa cgaagcggcg tcgaagcctg taaagcggcg gtgcacaatc
      421 ttctcgcgca acgcgtcagt gggctgatca ttaactatcc gctggatgac caggatgcca
      481 ttgctgtgga agctgcctgc actaatgttc cggcgttatt tcttgatgtc tctgaccaga
      541 cacccatcaa cagtattatt ttctcccatg aagacggtac gcgactgggc gtggagcatc
      601 tggtcgcatt gggtcaccag caaatcgcgc tgttagcggg cccattaagt tctgtctcgg
      661 cgcgtctgcg tctggctggc tggcataaat atctcactcg caatcaaatt cagccgatag
      721 cggaacggga aggcgactgg agtgccatgt ccggttttca acaaaccatg caaatgctga
      781 atgagggcat cgttcccact gcgatgctgg ttgccaacga tcagatggcg ctgggcgcaa
      841 tgcgcgccat taccgagtcc gggctgcgcg ttggtgcgga tatctcagta gtgggatacg
      901 acgataccga agacagctca tgttatatcc cgccgttaac caccatcaaa caggattttc
      961 gcctgctggg gcaaaccagc gtggaccgct tgctgcaact ctctcagggc caggcggtga
     1021 agggcaatca gctgttgccc gtctcactgg tgaaaagaaa aaccaccctg gcgcccaata
     1081 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     1141 cccgactgga aagcgggcag tgagcgcaac gcaattaatg taagttagct cactcattag
     1201 gcacaattct catgtttgac agcttatcat cgactgcacg gtgcaccaat gcttctggcg
     1261 tcaggcagcc atcggaagct gtggtatggc tgtgcaggtc gtaaatcact gcataattcg
     1321 tgtcgctcaa ggcgcactcc cgttctggat aatgtttttt gcgccgacat cataacggtt
     1381 ctggcaaata ttctgaaatg agctgttgac aattaatcat cggctcgtat aatgtgtgga
     1441 attgtgagcg gataacaatt tcacacagga aacagccagt ccgtttaggt gttttcacga
     1501 gcacttcacc aacaaggacc atagcatatg aaaatcgaag aaggtaaact ggtaatctgg
     1561 attaacggcg ataaaggcta taacggtctc gctgaagtcg gtaagaaatt cgagaaagat
     1621 accggaatta aagtcaccgt tgagcatccg gataaactgg aagagaaatt cccacaggtt
     1681 gcggcaactg gcgatggccc tgacattatc ttctgggcac acgaccgctt tggtggctac
     1741 gctcaatctg gcctgttggc tgaaatcacc ccggacaaag cgttccagga caagctgtat
     1801 ccgtttacct gggatgccgt acgttacaac ggcaagctga ttgcttaccc gatcgctgtt
     1861 gaagcgttat cgctgattta taacaaagat ctgctgccga acccgccaaa aacctgggaa
     1921 gagatcccgg cgctggataa agaactgaaa gcgaaaggta agagcgcgct gatgttcaac
     1981 ctgcaagaac cgtacttcac ctggccgctg attgctgctg acgggggtta tgcgttcaag
     2041 tatgaaaacg gcaagtacga cattaaagac gtgggcgtgg ataacgctgg cgcgaaagcg
     2101 ggtctgacct tcctggttga cctgattaaa aacaaacaca tgaatgcaga caccgattac
     2161 tccatcgcag aagctgcctt taataaaggc gaaacagcga tgaccatcaa cggcccgtgg
     2221 gcatggtcca acatcgacac cagcaaagtg aattatggtg taacggtact gccgaccttc
     2281 aagggtcaac catccaaacc gttcgttggc gtgctgagcg caggtattaa cgccgccagt
     2341 ccgaacaaag agctggcaaa agagttcctc gaaaactatc tgctgactga tgaaggtctg
     2401 gaagcggtta ataaagacaa accgctgggt gccgtagcgc tgaagtctta cgaggaagag
     2461 ttggcgaaag atccacgtat tgccgccact atggaaaacg cccagaaagg tgaaatcatg
     2521 ccgaacatcc cgcagatgtc cgctttctgg tatgccgtgc gtactgcggt gatcaacgcc
     2581 gccagcggtc gtcagactgt cgatgaagcc ctgaaagacg cgcagactaa ttcgagctcg
     2641 aacaacaaca acaataacaa taacaacaac ctcgggcacc accaccacca ccacgaaaac
     2701 ctgtacttcc aaggcgcagg cttaatggaa gcaaacaaac gacaaattgt ggaggtcgat
     2761 gggattaagt cctacttttt cccccatctc gcccattacc tcgcgagcaa tgacgagctg
     2821 ttggtcaaca acatagcaca ggcgaacaag cttgcagctt tcgtcttggg agccaccgac
     2881 aaacgcccaa gcaatgaaga aattgcggag atgatcctgc caaatgactc cagtgcctac
     2941 gtgcttgctg ctggcatgga cgtttgcctt attctcgggg atgacttccg accaaagttc
     3001 gattctggcg ctgagaagct gagtcagctt ggacaagctc atgatttggc tccaatcatc
     3061 gatgatgaaa agaaaatctc gatgctcgcc cgaaaaacaa agctaaagaa gtcgaatgac
     3121 gccaagattc tgcaagtctt gctcaaagtt ctcggtgcgg aagaagcgga agagaagttt
     3181 gtcgagctct cggaactgtc gagcgccttg gatcttgatt tcgacgtcta tgtcttggca
     3241 aaactgcttg gatttgcatc ggaggagctc caggaagaaa ttgaaatcat ccgtgacaac
     3301 gtcacagatg cgtttgaggc ctgcaagccg ttgttgaaga agctgatgat cgagggaccg
     3361 aagattgatt ctgtggaccc attcacccaa cttctgttaa ctccacaaga agaatctatc
     3421 gaaaaagctg tatcccacat cgttgctcgc ttcgaagaag ccagcgccgt cgaggatgat
     3481 gaatcactcg tcctgaaatc tcagctagga tatcaattga ttttcctcgt cgttcgctca
     3541 cttgctgatg gaaagcgtga tgcttccagg acaatccaat cgttgatgcc gtcgagtgtc
     3601 cgagcggaag tgtttcccgg attgcaacgg agtgttttca aaagtgccgt gttcctcgca
     3661 agccatatca ttcaagtctt tttgggatcc atgaagtcat tcgaggattg ggcgtttgtt
     3721 ggcctcgctg aagacttgga gagcacgtgg agaagaagag caatcgctga attgctgaaa
     3781 aagttcagaa tctctgttct cgagcagtgc ttttctcaac caatcccact gctccctcaa
     3841 agcgagttga acaacgaaac tgtcatcgag aatgtgaata atgctctcca atttgcattg
     3901 tggatcacag agttctacgg atcggagagt gagaagaaat cactcaatca gcttcaattc
     3961 ctaagcccaa aatccaagaa tcttcttgtt gatagcttca aaaagttcgc ccaaggactg
     4021 gattctaaag atcacgtgaa tcgtatcatt gaatctctgg agaagagcag ttcgtcggaa
     4081 ccttctgcaa ctgcaaaaca gaccacaact tccaatggac cgaccaccgt ctctactgca
     4141 gctcaagtag taactgttga aaagatgcca ttcagtcgtc aaacgattcc atgtgaagga
     4201 acagacttgg cgaatgtgct caattctgca aagatcatcg gagaaagtgt taccgtggct
     4261 gcccatgatg tcattccgga gaaactaaat gcggagaaga atgacaatac tccatcaact
     4321 gcctcacctg ttcaattttc tagcgatggt tgggattctc cgacaaaatc tgttgctctg
     4381 ccaccgaaga tctcgacttt ggaggaagaa caagaagaag atacaacgat caccaaggtt
     4441 tctccgcaac cacaagaacg aactggaacc gcatggggat caggcgatgc gactccagtg
     4501 cctcttgcta ctccggttaa cgaatacaaa gttagtgggt tcggcgctgc gccagtcgct
     4561 tcgggattcg gacagtttgc cagcagcaac ggaacgtctg gaagaggctc ttatggaggt
     4621 ggtcgcggtg gcgatcgtgg aggaagagga gcatacggtg gcgatcgtgg acgtggaggt
     4681 tccggagatg gatctcgtgg atatcgtggt ggtgatcgag gaggacgcgg aagttacggc
     4741 gaaggatctc gtggttacca gggaggacgt gccggattct ttggtggatc ccgtggaggt
     4801 tcctaagacc cagctttctt gtacaaagtg gtgatatttc agaattcgga tcctctagag
     4861 tcgacctgca ggcaagcttg gcactggccg tcgttttaca acgtcgtgac tgggaaaacc
     4921 ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata
     4981 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggc
     5041 agcttggctg ttttggcgga tgagataaga ttttcagcct gatacagatt aaatcagaac
     5101 gcagaagcgg tctgataaaa cagaatttgc ctggcggcag tagcgcggtg gtcccacctg
     5161 accccatgcc gaactcagaa gtgaaacgcc gtagcgccga tggtagtgtg gggtctcccc
     5221 atgcgagagt agggaactgc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg
     5281 gcctttcgtt ttatctgttg tttgtcggtg aacgctctcc tgagtaggac aaatccgccg
     5341 ggagcggatt tgaacgttgc gaagcaacgg cccggagggt ggcgggcagg acgcccgcca
     5401 taaactgcca ggcatcaaat taagcagaag gccatcctga cggatggcct ttttgcgttt
     5461 ctacaaactc ttttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     5521 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     5581 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     5641 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     5701 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gtttcccaat
     5761 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca
     5821 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     5881 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     5941 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     6001 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     6061 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     6121 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     6181 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     6241 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     6301 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     6361 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     6421 gtaactgtca gaccaagttt actcatatat actttagatt gatttacccc ggttgataat
     6481 cagaaaagcc ccaaaaacag gaagattgta taagcaaata tttaaattgt aaacgttaat
     6541 attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc
     6601 gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt
     6661 ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa
     6721 accgtctatc agggcgatgg cccactacgt gaaccatcac ccaaatcaag ttttttgggg
     6781 tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga
     6841 cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct
     6901 agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat
     6961 gcgccgctac agggcgcgta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     7021 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     7081 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     7141 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     7201 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg
     7261 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     7321 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     7381 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     7441 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     7501 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     7561 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     7621 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     7681 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     7741 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     7801 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
     7861 gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg
     7921 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat
     7981 cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct
     8041 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct
     8101 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct
     8161 catcagcgtg gtcgtgcagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt
     8221 tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg
     8281 ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa
     8341 tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc
     8401 ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa
     8461 aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta
     8521 gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg
     8581 tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag
     8641 acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac
     8701 cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca
     8761 cccgtggcca ggacccaacg ctgcccgaaa tt
//
