LOCUS       sequence-335810-       11090 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway destination clone of RPS6KL1 (human) tagged with N-terminal 
            miniTurbo-V5 for generating protein-proximity networks using 
            proximity-dependent biotinylation proteomics.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11090)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 11090)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p663-UBC-miniTurbo-V5-RPS6KL1_IDG-K
FEATURES             Location/Qualifiers
     source          1..11090
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     496..516
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             737..970
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    1042..1163
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1079..1098)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1133..1152
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      1203..1338
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(1358..1377)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1359..1377)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1387..1404)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1387..1403)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1396..1418)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1545..2000
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1632..1651)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1842..1863
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2026..2130
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2131..2199,2200..2991)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      3162..3750
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3651..3670
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3904..3921
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4038..4059
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4074..4079,4080..4097,4098..4104)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4112..4128
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4117..4139
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4136..4152
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4136..4152
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     4171..4191
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        4173..4191
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        4219..4445
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             4446..4626
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4673..4798
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5291..5524
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5709..5753
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6020..6136
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    6170..6190
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        6190..7401
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     7238..7257
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    complement(7405..7425)
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     regulatory      7426..7435
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7438..8208
                     /codon_start=1
                     /product="N-terminally truncated mutant of the E. coli 
                     biotin ligase BioID engineered for improved catalytic 
                     efficiency (Branon et al., 2018)"
                     /label=miniTurbo
                     /translation="IPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGDACI
                     AEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLGADK
                     VRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWITLQ
                     EAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIF
                     GISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     CDS             8215..8256
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    8257..8281
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(9914..9938)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    9968..10556
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(10021..10041)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10439..10450)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        10570..11069
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(10605..10628)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     11002..11021
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             join(11090,1..599)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(join(11090,1..19))
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 tgaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac
       61 gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gatccggacc
      121 gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca
      181 tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga
      241 gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt
      301 cccggctggc cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc
      361 ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca
      421 gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg
      481 agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg
      541 acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgac
      601 gcccgcccca cgacccgcag cgcccgaccg aaaggagcgc acgaccccat gcatctcgag
      661 ggcccggtac ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta
      721 aaagaaaagg ggggactgga agggctagct cactcccaac gaagacaaga tctgcttttt
      781 gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
      841 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
      901 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
      961 atctctagca gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa
     1021 tgaatatcag agagtgagag gaacttgttt attgcagctt ataatggtta caaataaagc
     1081 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     1141 tccaaactca tcaatgtatc ttatcatgtc tggctctagc tatcccgccc ctaactccgc
     1201 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
     1261 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag
     1321 gaggcttttt tggaggccta gggacgtacc caattcgccc tatagtgagt cgtattacgc
     1381 gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact
     1441 taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac
     1501 cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg
     1561 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
     1621 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
     1681 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
     1741 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac
     1801 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
     1861 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat
     1921 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa
     1981 aatattaacg cttacaattt aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     2041 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     2101 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     2161 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     2221 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     2281 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     2341 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
     2401 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     2461 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     2521 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     2581 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     2641 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
     2701 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     2761 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     2821 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     2881 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     2941 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     3001 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     3061 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     3121 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     3181 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     3241 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     3301 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     3361 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     3421 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     3481 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     3541 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     3601 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     3661 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     3721 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     3781 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     3841 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     3901 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
     3961 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc
     4021 agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac
     4081 tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga
     4141 aacagctatg accatgatta cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag
     4201 ctggagctgc aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa
     4261 cgatgagtta gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg
     4321 aagtaaggtg gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg
     4381 gacgaaccac tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca
     4441 taaacgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
     4501 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
     4561 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
     4621 ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg
     4681 acgcaggact cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag
     4741 tacgccaaaa attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag
     4801 tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa
     4861 gaaaaaatat aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt
     4921 taatcctggc ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc
     4981 atcccttcag acaggatcag aagaacttag atcattatat aatacagtag caaccctcta
     5041 ttgtgtgcat caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga
     5101 agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag
     5161 gaggagatat gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg
     5221 aaccattagg agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag
     5281 cagtgggaat aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg
     5341 cagcgtcaat gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc
     5401 agaacaattt gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg
     5461 gcatcaagca gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc
     5521 tcctggggat ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg
     5581 ctagttggag taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg
     5641 acagagaaat taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc
     5701 agcaagaaaa gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt
     5761 ggtttaacat aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct
     5821 tggtaggttt aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat
     5881 attcaccatt atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag
     5941 gaatagaaga agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat
     6001 ctcgacggta tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg
     6061 aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta
     6121 caaaattcaa aattttatcg tccagtgtgg tggaattctg cagatatcac aactttgtat
     6181 agaaaagttg gcctccgcgc cgggttttgg cgcctcccgc gggcgccccc ctcctcacgg
     6241 cgagcgctgc cacgtcagac gaagggcgca gcgagcgtcc tgatccttcc gcccggacgc
     6301 tcaggacagc ggcccgctgc tcataagact cggccttaga accccagtat cagcagaagg
     6361 acattttagg acgggacttg ggtgactcta gggcactggt tttctttcca gagagcggaa
     6421 caggcgagga aaagtagtcc cttctcggcg attctgcgga gggatctccg tggggcggtg
     6481 aacgccgatg attatataag gacgcgccgg gtgtggcaca gctagttccg tcgcagccgg
     6541 gatttgggtc gcggttcttg tttgtggatc gctgtgatcg tcacttggtg agtagcgggc
     6601 tgctgggctg gccggggctt tcgtggccgc cgggccgctc ggtgggacgg aagcgtgtgg
     6661 agagaccgcc aagggctgta gtctgggtcc gcgagcaagg ttgccctgaa ctgggggttg
     6721 gggggagcgc agcaaaatgg cggctgttcc cgagtcttga atggaagacg cttgtgaggc
     6781 gggctgtgag gtcgttgaaa caaggtgggg ggcatggtgg gcggcaagaa cccaaggtct
     6841 tgaggccttc gctaatgcgg gaaagctctt attcgggtga gatgggctgg ggcaccatct
     6901 ggggaccctg acgtgaagtt tgtcactgac tggagaactc ggtttgtcgt ctgttgcggg
     6961 ggcggcagtt atggcggtgc cgttgggcag tgcacccgta cctttgggag cgcgcgccct
     7021 cgtcgtgtcg tgacgtcacc cgttctgttg gcttataatg cagggtgggg ccacctgccg
     7081 gtaggtgtgc ggtaggcttt tctccgtcgc aggacgcagg gttcgggcct agggtaggct
     7141 ctcctgaatc gacaggcgcc ggacctctgg tgaggggagg gataagtgag gcgtcagttt
     7201 ctttggtcgg ttttatgtac ctatcttctt aagtagctga agctccggtt ttgaactatg
     7261 cgctcggggt tggcgagtgt gttttgtgaa gttttttagg caccttttga aatgtaatca
     7321 tttgggtcaa tatgtaattt tcagtgttag actagtaaat tgtccgctaa attctggccg
     7381 tttttggctt ttttgttaga cggccaactt ttgtatacaa agtgggccac catgggaatc
     7441 ccgctgctga acgctaaaca gattctggga cagctggacg gcgggagcgt ggcagtcctg
     7501 cctgtggtcg actccaccaa tcagtacctg ctggatcgaa tcggcgagct gaagagtggg
     7561 gatgcttgca ttgcagaata tcagcaggca gggagaggaa gcagagggag gaaatggttc
     7621 tctccttttg gagctaacct gtacctgagt atgttttggc gcctgaagcg gggaccagca
     7681 gcaatcggcc tgggcccggt catcggaatt gtcatggcag aagcgctgcg aaagctggga
     7741 gcagacaagg tgcgagtcaa atggcccaat gacctgtatc tgcaggatag aaagctggca
     7801 ggcatcctgg tggagctggc cggaataaca ggcgatgctg cacagatcgt cattggcgcc
     7861 gggattaacg tggctatgag gcgcgtggag gaaagcgtgg tcaatcaggg ctggatcaca
     7921 ctgcaggaag cagggattaa cctggacagg aatactctgg ccgctatgct gatccgagag
     7981 ctgcgggcag ccctggaact gttcgagcag gaaggcctgg ctccatatct gtcacggtgg
     8041 gagaagctgg ataacttcat caatagaccc gtgaagctga tcattgggga caaagagatt
     8101 ttcgggatta gccgggggat tgataaacag ggagccctgc tgctggaaca ggacggagtt
     8161 atcaaaccct ggatgggcgg agaaatcagt ctgcggtctg ccgaaaaggc tagcggcaag
     8221 cccatcccca accccctgct gggcctggac agcaccacaa gtttgtacaa aaaagcaggc
     8281 tccaccatga gcctggtggc ctgtgagtgc ctgcccagcc ccggcctgga gcctgagcct
     8341 tgctcacgag cacggtccca agctcacgtg tacctggagc agattcgcaa cagggtggct
     8401 ctgggagtgc ctgacatgac aaaacgtgac tatctggtgg atgcggccac gcagatccgg
     8461 ctggccctgg agcgcgatgt tagtgaggac tatgaggcgg ccttcaacca ctatcagaat
     8521 ggcgtggacg tgctgctccg tggcatacac gttgacccca acaaggagcg acgtgaggct
     8581 gtgaagctga aaattaccaa atacctgcgg cgggcagagg agatcttcaa ctgccacctg
     8641 cagcggccgc tgagcagtgg agccagcccc agcgcggtgc agctggtcca ggacccggca
     8701 accggaggga cctttgtggt gaagagccta cccaggtgcc acatggtgag cagggagcgg
     8761 ctgaccatca tcccacacgg agtcccctac atgacgaagc tgctcaggta ctttgtgagc
     8821 gaggactcca tcttcctgca cctggagcat gtgcaaggag gcactctctg gtcccacctg
     8881 ctctcccagg cgcactcccg acattctggg ctcagctctg gctctaccca ggagaggatg
     8941 aaggctcagc tcaaccccca cctcaacctc ctgaccccag cgaggcttcc ctcaggccat
     9001 gcccctggcc aggacagaat cgccctggag cctcctagga cttctccgaa ccttctccta
     9061 gctggggagg ccccatccac cagaccccag agggaggctg aaggtgaacc cacagccagg
     9121 accagcacct ctggctcctc ggaccttcca aaggccccag gtggccacct gcaccttcaa
     9181 gctaggaggg ctggccagaa ctcagacgct gggccccctc gggggctcac ttgggttcct
     9241 gagggggccg gcccggtgct agggggctgt ggccgaggca tggatcagag ctgcctgtca
     9301 gcagatgggg ccggccgggg ctgtggcagg gccacctgga gtgtgagaga ggagcaggtg
     9361 aagcagtggg cggcagagat gctggtagcg ctggaggcgc tgcacgagca gggggtgctg
     9421 tgccgggacc tccaccccgg gaacctgctc ctggaccagg caggtcacat ccggctcaca
     9481 tattttggcc agtggtcaga ggtggagccc cagtgctgcg gggaggccgt ggacaatctc
     9541 tacagcgccc cagaggtggg tgggatttcc gagctgacgg aagcctgtga ctggtggagc
     9601 tttgggtctc tactgtatga actgctgacg ggaatggcac tgtcccagag ccacccttca
     9661 ggaatccagg cccacaccca gctccagctg cccgagtggc tcagtcgccc agcggcctct
     9721 ctgctgactg agctgctgca gttcgagcct acccggcgcc tgggcatggg agaaggtggt
     9781 gtcagcaaac tcaagtccca tccctttttc agtaccatcc aatggagcaa gctggtgggg
     9841 acatctctgg agactcatct caggacactg atccactggc tcagtggacc caaaccagac
     9901 tgtcctggct aggacccagc tttcttgtac aaagtggtga tatccagcac agtggcggcc
     9961 gctcgacaat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
    10021 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc
    10081 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga
    10141 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac
    10201 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc
    10261 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc
    10321 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aagctgacgt cctttccatg
    10381 gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc
    10441 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc
    10501 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctgg
    10561 aattctaccg ggtaggggag gcgcttttcc caaggcagtc tggagcatgc gctttagcag
    10621 ccccgctggg cacttggcgc tacacaagtg gcctctggcc tcgcacacat tccacatcca
    10681 ccggtaggcg ccaaccggct ccgttctttg gtggcccctt cgcgccacct tctactcctc
    10741 ccctagtcag gaagttcccc cccgccccgc agctcgcgtc gtgcaggacg tgacaaatgg
    10801 aagtagcacg tctcactagt ctcgtgcaga tggacagcac cgctgagcaa tggaagcggg
    10861 taggcctttg gggcagcggc caatagcagc tttgctcctt cgctttctgg gctcagaggc
    10921 tgggaagggg tgggtccggg ggcgggctca ggggcgggct caggggcggg gcgggcgccc
    10981 gaaggtcctc cggaggcccg gcattctgca cgcttcaaaa gcgcacgtct gccgcgctgt
    11041 tctcctcttc ctcatctccg ggcctttcga cctgcagccc aagcttacca
//
