LOCUS       sequence-335812-       11531 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway destination clone of SCYL3 (human) tagged with N-terminal 
            miniTurbo-V5 for generating protein-proximity networks using 
            proximity-dependent biotinylation proteomics.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11531)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 11531)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p663-UBC-miniTurbo-V5-SCYL3_IDG-K
FEATURES             Location/Qualifiers
     source          1..11531
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     382..402
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             623..856
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    928..1049
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(965..984)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1019..1038
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      1089..1224
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(1244..1263)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1245..1263)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1273..1290)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1273..1289)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1282..1304)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1431..1886
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1518..1537)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1728..1749
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        1912..2016
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2017..2085,2086..2877)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      3048..3636
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3537..3556
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    3924..3945
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3960..3965,3966..3983,3984..3990)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3998..4014
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4003..4025
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4022..4038
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4022..4038
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     4057..4077
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        4059..4077
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        4105..4331
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             4332..4512
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4559..4684
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5177..5410
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5595..5639
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5906..6022
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    6056..6076
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        6076..7287
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     7124..7143
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    complement(7291..7311)
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     regulatory      7312..7321
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7324..8094
                     /codon_start=1
                     /product="N-terminally truncated mutant of the E. coli 
                     biotin ligase BioID engineered for improved catalytic 
                     efficiency (Branon et al., 2018)"
                     /label=miniTurbo
                     /translation="IPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGDACI
                     AEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLGADK
                     VRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWITLQ
                     EAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIF
                     GISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     CDS             8101..8142
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    8143..8167
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(10241..10265)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    10295..10883
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(10348..10368)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10766..10777)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        10897..11396
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(10932..10955)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     11329..11348
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             join(11417..11531,1..485)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(11417..11436)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc
       61 tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc
      121 cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga
      181 gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca
      241 aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc
      301 tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct
      361 tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca
      421 ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg
      481 cctgacgccc gccccacgac ccgcagcgcc cgaccgaaag gagcgcacga ccccatgcat
      541 ctcgagggcc cggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac
      601 tttttaaaag aaaagggggg actggaaggg ctagctcact cccaacgaag acaagatctg
      661 ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc
      721 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
      781 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
      841 tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca
      901 aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa
      961 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     1021 ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa
     1081 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     1141 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt
     1201 agtgaggagg cttttttgga ggcctaggga cgtacccaat tcgccctata gtgagtcgta
     1261 ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac
     1321 ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc
     1381 ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggg acgcgccctg
     1441 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
     1501 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     1561 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
     1621 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg
     1681 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
     1741 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt
     1801 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt
     1861 taacaaaata ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc
     1921 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     1981 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     2041 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     2101 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     2161 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     2221 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
     2281 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     2341 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     2401 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     2461 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     2521 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
     2581 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     2641 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     2701 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     2761 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     2821 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     2881 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     2941 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     3001 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     3061 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     3121 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     3181 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     3241 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     3301 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     3361 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     3421 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     3481 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     3541 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     3601 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     3661 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     3721 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     3781 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
     3841 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa
     3901 gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct
     3961 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac
     4021 acaggaaaca gctatgacca tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa
     4081 caaaagctgg agctgcaagc ttaatgtagt cttatgcaat actcttgtag tcttgcaaca
     4141 tggtaacgat gagttagcaa catgccttac aaggagagaa aaagcaccgt gcatgccgat
     4201 tggtggaagt aaggtggtac gatcgtgcct tattaggaag gcaacagacg ggtctgacat
     4261 ggattggacg aaccactgaa ttgccgcatt gcagagatat tgtatttaag tgcctagctc
     4321 gatacataaa cgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     4381 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
     4441 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
     4501 aaatctctag cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac cagaggagct
     4561 ctctcgacgc aggactcggc ttgctgaagc gcgcacggca agaggcgagg ggcggcgact
     4621 ggtgagtacg ccaaaaattt tgactagcgg aggctagaag gagagagatg ggtgcgagag
     4681 cgtcagtatt aagcggggga gaattagatc gcgatgggaa aaaattcggt taaggccagg
     4741 gggaaagaaa aaatataaat taaaacatat agtatgggca agcagggagc tagaacgatt
     4801 cgcagttaat cctggcctgt tagaaacatc agaaggctgt agacaaatac tgggacagct
     4861 acaaccatcc cttcagacag gatcagaaga acttagatca ttatataata cagtagcaac
     4921 cctctattgt gtgcatcaaa ggatagagat aaaagacacc aaggaagctt tagacaagat
     4981 agaggaagag caaaacaaaa gtaagaccac cgcacagcaa gcggccgctg atcttcagac
     5041 ctggaggagg agatatgagg gacaattgga gaagtgaatt atataaatat aaagtagtaa
     5101 aaattgaacc attaggagta gcacccacca aggcaaagag aagagtggtg cagagagaaa
     5161 aaagagcagt gggaatagga gctttgttcc ttgggttctt gggagcagca ggaagcacta
     5221 tgggcgcagc gtcaatgacg ctgacggtac aggccagaca attattgtct ggtatagtgc
     5281 agcagcagaa caatttgctg agggctattg aggcgcaaca gcatctgttg caactcacag
     5341 tctggggcat caagcagctc caggcaagaa tcctggctgt ggaaagatac ctaaaggatc
     5401 aacagctcct ggggatttgg ggttgctctg gaaaactcat ttgcaccact gctgtgcctt
     5461 ggaatgctag ttggagtaat aaatctctgg aacagatttg gaatcacacg acctggatgg
     5521 agtgggacag agaaattaac aattacacaa gcttaataca ctccttaatt gaagaatcgc
     5581 aaaaccagca agaaaagaat gaacaagaat tattggaatt agataaatgg gcaagtttgt
     5641 ggaattggtt taacataaca aattggctgt ggtatataaa attattcata atgatagtag
     5701 gaggcttggt aggtttaaga atagtttttg ctgtactttc tatagtgaat agagttaggc
     5761 agggatattc accattatcg tttcagaccc acctcccaac cccgagggga cccgacaggc
     5821 ccgaaggaat agaagaagaa ggtggagaga gagacagaga cagatccatt cgattagtga
     5881 acggatctcg acggtatcgg ttaactttta aaagaaaagg ggggattggg gggtacagtg
     5941 caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac
     6001 aaattacaaa attcaaaatt ttatcgtcca gtgtggtgga attctgcaga tatcacaact
     6061 ttgtatagaa aagttggcct ccgcgccggg ttttggcgcc tcccgcgggc gcccccctcc
     6121 tcacggcgag cgctgccacg tcagacgaag ggcgcagcga gcgtcctgat ccttccgccc
     6181 ggacgctcag gacagcggcc cgctgctcat aagactcggc cttagaaccc cagtatcagc
     6241 agaaggacat tttaggacgg gacttgggtg actctagggc actggttttc tttccagaga
     6301 gcggaacagg cgaggaaaag tagtcccttc tcggcgattc tgcggaggga tctccgtggg
     6361 gcggtgaacg ccgatgatta tataaggacg cgccgggtgt ggcacagcta gttccgtcgc
     6421 agccgggatt tgggtcgcgg ttcttgtttg tggatcgctg tgatcgtcac ttggtgagta
     6481 gcgggctgct gggctggccg gggctttcgt ggccgccggg ccgctcggtg ggacggaagc
     6541 gtgtggagag accgccaagg gctgtagtct gggtccgcga gcaaggttgc cctgaactgg
     6601 gggttggggg gagcgcagca aaatggcggc tgttcccgag tcttgaatgg aagacgcttg
     6661 tgaggcgggc tgtgaggtcg ttgaaacaag gtggggggca tggtgggcgg caagaaccca
     6721 aggtcttgag gccttcgcta atgcgggaaa gctcttattc gggtgagatg ggctggggca
     6781 ccatctgggg accctgacgt gaagtttgtc actgactgga gaactcggtt tgtcgtctgt
     6841 tgcgggggcg gcagttatgg cggtgccgtt gggcagtgca cccgtacctt tgggagcgcg
     6901 cgccctcgtc gtgtcgtgac gtcacccgtt ctgttggctt ataatgcagg gtggggccac
     6961 ctgccggtag gtgtgcggta ggcttttctc cgtcgcagga cgcagggttc gggcctaggg
     7021 taggctctcc tgaatcgaca ggcgccggac ctctggtgag gggagggata agtgaggcgt
     7081 cagtttcttt ggtcggtttt atgtacctat cttcttaagt agctgaagct ccggttttga
     7141 actatgcgct cggggttggc gagtgtgttt tgtgaagttt tttaggcacc ttttgaaatg
     7201 taatcatttg ggtcaatatg taattttcag tgttagacta gtaaattgtc cgctaaattc
     7261 tggccgtttt tggctttttt gttagacggc caacttttgt atacaaagtg ggccaccatg
     7321 ggaatcccgc tgctgaacgc taaacagatt ctgggacagc tggacggcgg gagcgtggca
     7381 gtcctgcctg tggtcgactc caccaatcag tacctgctgg atcgaatcgg cgagctgaag
     7441 agtggggatg cttgcattgc agaatatcag caggcaggga gaggaagcag agggaggaaa
     7501 tggttctctc cttttggagc taacctgtac ctgagtatgt tttggcgcct gaagcgggga
     7561 ccagcagcaa tcggcctggg cccggtcatc ggaattgtca tggcagaagc gctgcgaaag
     7621 ctgggagcag acaaggtgcg agtcaaatgg cccaatgacc tgtatctgca ggatagaaag
     7681 ctggcaggca tcctggtgga gctggccgga ataacaggcg atgctgcaca gatcgtcatt
     7741 ggcgccggga ttaacgtggc tatgaggcgc gtggaggaaa gcgtggtcaa tcagggctgg
     7801 atcacactgc aggaagcagg gattaacctg gacaggaata ctctggccgc tatgctgatc
     7861 cgagagctgc gggcagccct ggaactgttc gagcaggaag gcctggctcc atatctgtca
     7921 cggtgggaga agctggataa cttcatcaat agacccgtga agctgatcat tggggacaaa
     7981 gagattttcg ggattagccg ggggattgat aaacagggag ccctgctgct ggaacaggac
     8041 ggagttatca aaccctggat gggcggagaa atcagtctgc ggtctgccga aaaggctagc
     8101 ggcaagccca tccccaaccc cctgctgggc ctggacagca ccacaagttt gtacaaaaaa
     8161 gcaggctcca ccatgggatc agagaacagt gctttaaaga gctatacact gagagaacca
     8221 ccatttacct taccctctgg acttgctgtt tatcccgctg tactgcaaga tggcaaattt
     8281 gcttcagttt ttgtgtataa gagagaaaat gaagacaagg ttaataaagc tgccaagcat
     8341 ttgaagacac ttcgtcaccc ttgcttgcta agatttttat cttgtactgt ggaagcggat
     8401 ggcattcatc ttgtcactga gcgagtacag cccctggaag tggctttgga aacattgtct
     8461 tctgcagagg tctgtgctgg gatctatgac atattgctgg ctcttatctt ccttcatgac
     8521 agaggacacc taacacacaa taatgtctgt ttatcatctg tgtttgtgag tgaagatgga
     8581 cactggaagc taggaggaat ggaaactgtt tgtaaagttt ctcaggccac accagagttt
     8641 ctgaggagta ttcagtcaat aagagaccca gcatctatcc ctcctgaaga gatgtctcca
     8701 gaattcacaa ctctcccaga gtgtcatgga catgcccggg atgccttttc atttggaaca
     8761 ttggtggaaa gtttgctcac aatcttaaat gaacaggttt cagcggatgt tctctccagc
     8821 tttcaacaga ccttgcactc aactttgctg aatcccattc caaaatgtcg gccagcgctc
     8881 tgcaccttac tatctcatga cttcttcaga aatgattttc tggaagttgt gaatttcttg
     8941 aaaagtttaa cattgaagag tgaagaggag aaaacggaat tctttaaatt tctgctggac
     9001 agagtcagct gcttgtcaga ggaattgata gcttcaaggt tggtgcctct tctgcttaat
     9061 cagttggtgt ttgcagagcc agtggctgtt aagagttttc ttccttatct gcttggcccc
     9121 aaaaaagatc atgcgcaggg agaaactcct tgcttgctct caccagccct gttccagtca
     9181 cgggtgatcc ccgtgcttct ccagttgttt gaagttcatg aagagcatgt gcggatggtg
     9241 ctgctgtctc acatcgaggc ctacgtggag cacttcactc aggagcagct gaagaaagtc
     9301 atcttgccac aggttttgct gggcctgcgt gatactagtg attccattgt ggcaattact
     9361 ctgcatagcc tagcagtgct ggtctctctg cttggaccag aggtggttgt gggaggagaa
     9421 cgaaccaaga tcttcaaacg cactgcccca agttttacta aaaatactga cctttctcta
     9481 gaaggtgatc cattttctca gcctattaaa tttcccataa acggactctc agatgtaaaa
     9541 aatacttcgg aggacagtga aaacttccca tcaagttcta aaaagtctga ggagtggcct
     9601 gactggagtg aacctgagga gcctgaaaat caaactgtca acatacagat ttggcctaga
     9661 gaaccttgtg atgatgtcaa gtcccagtgc actaccttgg atgtggaaga gtcatcttgg
     9721 gatgactgcg agcccagcag cttagatact aaagtaaacc caggaggtgg aatcactgct
     9781 acaaaacctg ttacctcagc ggagcagaag cctattcctg ctttgctttc actcactgaa
     9841 gagtctatgc cttggaaatc aagcttaccc caaaagatta gccttgtaca aaggggggat
     9901 gacgcagacc aaatcgagcc gccaaaagtg tcatcacaag aaaggcccct taaggttcca
     9961 tcagaacttg gtttaggaga ggaattcacc attcaagtaa aaaagaagcc agtaaaagat
    10021 cctgagatgg attggtttgc tgatatgatc ccagaaatta agccttctgc tgcttttctt
    10081 atattacctg aactgaggac agaaatggtc ccaaaaaagg atgatgtctc cccagtgatg
    10141 cagttttcct caaaatttgc tgcagcagaa attactgagg gagaggctga aggctgggaa
    10201 gaagaagggg agctgaactg ggaagataat aactggtagg acccagcttt cttgtacaaa
    10261 gtggtgatat ccagcacagt ggcggccgct cgacaatcaa cctctggatt acaaaatttg
    10321 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc
    10381 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta
    10441 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt
    10501 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca
    10561 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc
    10621 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt
    10681 gtcggggaag ctgacgtcct ttccatggct gctcgcctgt gttgccacct ggattctgcg
    10741 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg
    10801 cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat
    10861 ctccctttgg gccgcctccc cgcctggaat tctaccgggt aggggaggcg cttttcccaa
    10921 ggcagtctgg agcatgcgct ttagcagccc cgctgggcac ttggcgctac acaagtggcc
    10981 tctggcctcg cacacattcc acatccaccg gtaggcgcca accggctccg ttctttggtg
    11041 gccccttcgc gccaccttct actcctcccc tagtcaggaa gttccccccc gccccgcagc
    11101 tcgcgtcgtg caggacgtga caaatggaag tagcacgtct cactagtctc gtgcagatgg
    11161 acagcaccgc tgagcaatgg aagcgggtag gcctttgggg cagcggccaa tagcagcttt
    11221 gctccttcgc tttctgggct cagaggctgg gaaggggtgg gtccgggggc gggctcaggg
    11281 gcgggctcag gggcggggcg ggcgcccgaa ggtcctccgg aggcccggca ttctgcacgc
    11341 ttcaaaagcg cacgtctgcc gcgctgttct cctcttcctc atctccgggc ctttcgacct
    11401 gcagcccaag cttaccatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga
    11461 cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca
    11521 caccgtcgat c
//
