LOCUS       sequence-335813-        4430 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway-compatible Entry vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4430)
  AUTHORS   Weller B, Lin CW, Pogoutse O, Sauer M, Marin-de la Rosa N, Strobel 
            A, Young V, Knapp JJ, Rayhan A, Falter C, Kim DK, Roth FP, 
            Falter-Braun P
  TITLE     A resource of human coronavirus protein-coding sequences in a 
            flexible, multipurpose Gateway Entry clone collection.
  JOURNAL   G3 (Bethesda). 2023 Jul 5;13(7):jkad105. doi: 
            10.1093/g3journal/jkad105.
  PUBMED    37267226
REFERENCE   2  (bases 1 to 4430)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda)."; date: "2023-07-5"; volume: "13(7):jkad105. doi"; 
            pages: " 10.1093/g3journal/jkad105"
COMMENT     Sequence Label: pEntry_SARS-CoV-1-nsp14
FEATURES             Location/Qualifiers
     source          1..4430
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1741..1840)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1857..1876)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1858..1876)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1881..1897)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1881..1897)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2328..3119
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3212..3800
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3701..3720
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3954..3971
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4130..4157)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4249..4335)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4384..4406
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4398..4415
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4399..4415
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcagaag ggccgtcaag gccaggatcc
      121 atgggatccg cagaaaatgt aactggactt tttaaggact gtagtaagat cattactggt
      181 cttcatccta cacaggcacc tacacacctc agcgttgata taaagttcaa gactgaagga
      241 ttatgtgttg acataccagg cataccaaag gacatgacct accgtagact catctctatg
      301 atgggtttca aaatgaatta ccaagtcaat ggttacccta atatgtttat cacccgcgaa
      361 gaagctattc gtcacgttcg tgcgtggatt ggctttgatg tagagggctg tcatgcaact
      421 agagatgctg tgggtactaa cctacctctc cagctaggat tttctacagg tgttaactta
      481 gtagctgtac cgactggtta tgttgacact gaaaataaca cagaattcac cagagttaat
      541 gcaaaacctc caccaggtga ccagtttaaa catcttatac cactcatgta taaaggcttg
      601 ccctggaatg tagtgcgtat taagatagta caaatgctca gtgatacact gaaaggattg
      661 tcagacagag tcgtgttcgt cctttgggcg catggctttg agcttacatc aatgaagtac
      721 tttgtcaaga ttggacctga aagaacgtgt tgtctgtgtg acaaacgtgc aacttgcttt
      781 tctacttcat cagatactta tgcctgctgg aatcattctg tgggttttga ctatgtctat
      841 aacccattta tgattgatgt tcagcagtgg ggctttacgg gtaaccttca gagtaaccat
      901 gaccaacatt gccaggtaca tggaaatgca catgtggcta gttgtgatgc tatcatgact
      961 agatgtttag cagtccatga gtgctttgtt aagcgcgttg attggtctgt tgaataccct
     1021 attataggag atgaactgag ggttaattct gcttgcagaa aagtacaaca catggttgtg
     1081 aagtctgcat tgcttgctga taagtttcca gttcttcatg acattggaaa tccaaaggct
     1141 atcaagtgtg tgcctcaggc tgaagtagaa tggaagttct acgatgctca gccatgtagt
     1201 gacaaagctt acaaaataga ggaactcttc tattcttatg ctacacatca cgataaattc
     1261 actgatggtg tttgtttgtt ttggaattgt aacgttgatc gttacccagc caatgcaatt
     1321 gtgtgtaggt ttgacacaag agtcttgtca aacttgaact taccaggctg tgatggtggt
     1381 agtttgtatg tgaataagca tgcattccac actccagctt tcgataaaag tgcatttact
     1441 aatttaaagc aattgccttt cttttactat tctgatagtc cttgtgagtc tcatggcaaa
     1501 caagtagtgt cggatattga ttatgttcca ctcaaatctg ctacgtgtat tacacgatgc
     1561 aatttaggtg gtgctgtttg cagacaccat gcaaatgagt accgacagta cttggatgca
     1621 tataatatga tgatttctgc tggatttagc ctatggattt acaaacaatt tgatacttat
     1681 aacctgtgga atacatttac caggttacag cttaattaag acgcgatcgc ggcctcatgg
     1741 gcccagcttt cttgtacaaa gttggcatta taaaaaataa ttgctcatca atttgttgca
     1801 acgaacaggt cactatcagt caaaataaaa tcattatttg ccatccagct gatatcccct
     1861 atagtgagtc gtattacatg gtcatagctg tttcctggca gctctggccc gtgtctcaaa
     1921 atctctgatg ttacattgca caagataaaa atatatcatc atgcctcctc tagaccagcc
     1981 aggacagaaa tgcctcgact tcgctgctac ccaaggttgc cgggtgacgc acaccgtgga
     2041 aacggatgaa ggcacgaacc cagtggacat aagcctgttc ggttcgtaag ctgtaatgca
     2101 agtagcgtat gcgctcacgc aactggtcca gaaccttgac cgaacgcagc ggtggtaacg
     2161 gcgcagtggc ggttttcatg gcttgttatg actgtttttt tggggtacag tctatgcctc
     2221 gggcatccaa gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta tggagcagca
     2281 acgatgttac gcagcagggc agtcgcccta aaacaaagtt aaacattatg agggaagcgg
     2341 tgatcgccga agtatcgact caactatcag aggtagttgg cgtcatcgag cgccatctcg
     2401 aaccgacgtt gctggccgta catttgtacg gctccgcagt ggatggcggc ctgaagccac
     2461 acagtgatat tgatttgctg gttacggtga ccgtaaggct tgatgaaaca acgcggcgag
     2521 ctttgatcaa cgaccttttg gaaacttcgg cttcccctgg agagagcgag attctccgcg
     2581 ctgtagaagt caccattgtt gtgcacgacg acatcattcc gtggcgttat ccagctaagc
     2641 gcgaactgca atttggagaa tggcagcgca atgacattct tgcaggtatc ttcgagccag
     2701 ccacgatcga cattgatctg gctatcttgc tgacaaaagc aagagaacat agcgttgcct
     2761 tggtaggtcc agcggcggag gaactctttg atccggttcc tgaacaggat ctatttgagg
     2821 cgctaaatga aaccttaacg ctatggaact cgccgcccga ctgggctggc gatgagcgaa
     2881 atgtagtgct tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa atcgcgccga
     2941 aggatgtcgc tgccgactgg gcaatggagc gcctgccggc ccagtatcag cccgtcatac
     3001 ttgaagctag acaggcttat cttggacaag aagaagatcg cttggcctcg cgcgcagatc
     3061 agttggaaga atttgtccac tacgtgaaag gcgagatcac caaggtagtc ggcaaataac
     3121 cctcgagcca cccatgacca aaatccctta acgtgagtta cgcgtcgttc cactgagcgt
     3181 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     3241 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     3301 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     3361 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     3421 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     3481 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     3541 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     3601 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     3661 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     3721 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     3781 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     3841 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     3901 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     3961 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     4021 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     4081 acgcaattaa tacgcgtacc gctagccagg aagagtttgt agaaacgcaa aaaggccatc
     4141 cgtcaggatg gccttctgct tagtttgatg cctggcagtt tatggcgggc gtcctgcccg
     4201 ccaccctccg ggccgttgct tcacaacgtt caaatccgct cccggcggat ttgtcctact
     4261 caggagagcg ttcaccgaca aacaacagat aaaacgaaag gcccagtctt ccgactgagc
     4321 ctttcgtttt atttgatgcc tggcagttcc ctactctcgc gttaacgcta gcatggatgt
     4381 tttcccagtc acgacgttgt aaaacgacgg ccagtcttaa gctcgggccc
//
