LOCUS       sequence-33582-e         854 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 854)
  TITLE     Fitzgerald Lab Plasmids
REFERENCE   2  (bases 1 to 854)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pEF-BOS huTBK1 K38M Flag-His
FEATURES             Location/Qualifiers
     source          1..854
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(67..84)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(85..108)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(118..129)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 cgtgggagac ctgatactct caagggtccc aggtggggac cctcactcta gagtcgcggc
       61 cgctcagtgg tggtggtggt ggtgcttgtc atcgtcgtcc ttgtagtcct tgacgatcct
      121 tccttcaatg gatccaagac agtcaacgtt gcgaaggcca ccatccatgg ttaaagagcc
      181 aaacctttct aaaatgtggt tattttcagc aagttcttta accacccctt ccatctcttc
      241 ctttaatttc ttcataccaa gagtcatttc tactaatgtg ttagaacttg gataaattgg
      301 ggtcatggta tgtttgattc cactggaagc tgtaaacatt ttctgaggca gagtttcttg
      361 taactcatta gtatattctt gatattttga tacttcttct tcaatatcaa aacactgatt
      421 agtcagcgat aataactgtt tcctaagatg aagcatcttt cttatccatt cttctgactt
      481 attcaaaaat gcctcatact ttttaacaca ttcatctgta aagtgcgtca tagcttttgt
      541 ggcatggtaa tacagttttt gcttatcaaa tttgtggatt tgttcttcat tataagctaa
      601 tctacgttct gctttgtctt ttttgaactg atagtaaatc tctgtcatgc aatttaacag
      661 gacttgtagt ttttctacat ttctgtcttt cggatgagtg ccttcttgat gtgcccatgc
      721 gtctgccagt gatccacctg gagataatct gctgtcgata tcctgaagac tgggtttcta
      781 ttgttccctg agaactggaa agtctcaaca atttggtgtg tatgtctgaa atttcaccta
      841 actctgccgc ttcc
//
