LOCUS       sequence-335826-        1554 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of eGFP-CBP protein for chitin visualization.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1554)
  AUTHORS   Ricardo-Gonzalez RR, Kotas ME, Tenvooren I, Marquez DM, Fassett MS, 
            Lee J, Daniel SG, Bittinger K, Diaz RE, Fraser JS, Ansel KM, Spitzer
            MH, Liang H, Locksley RM
  TITLE     Type 2 innate immunity regulates hair follicle homeostasis to 
            control Demodex pathosymbionts
  JOURNAL   bioRxiv 2021.03.29.437438
REFERENCE   2  (bases 1 to 1554)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2021.03.29.437438"
COMMENT     Sequence Label: eGFP-CBP
FEATURES             Location/Qualifiers
     source          1..1554
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(1..3,4..6,7..717)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             754..1347
                     /codon_start=1
                     /gene="Mycobacterium xenopi gyrA"
                     /product="modified GyrA intein (Southworth et al., 1999)"
                     /label=Mxe GyrA intein
                     /note="N-terminal cleavage of this intein can be induced by
                     thiols."
                     /translation="CITGDALVALPEGESVRIADIVPGARPNSDNAIDLKVLDRHGNPV
                     LADRLFHSGEHPVYTVRTVEGLRVTGTANHPLLCLVDVAGVPTLLWKLIDEIKPGDYAV
                     IQRSAFSVDCAGFARGKPEFAPTTYTVGVPGLVRFLEAHHRDPDAQAIADELTDGRFYY
                     AKVASVTDAGVQPVYSLRVDTADHAFITNGFVSHA"
     CDS             1378..1533
                     /codon_start=1
                     /gene="Bacillus circulans chiA"
                     /product="chitin binding domain from chitinase A1 (Watanabe
                     et al., 1994)"
                     /label=CBD
                     /translation="TTNPGVSAWQVNTAYTAGQLVTYNGKTYKCLQPHTSLAGWEPSNV
                     PALWQLQ"
     CDS             1534..1551
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atggttagca aaggcgaaga actgtttacg ggcgtggtgc cgatcctggt ggaactggac
       61 ggtgatgtta atggccataa attttcagtc tcgggtgaag gcgaaggtga tgccacctat
      121 ggtaaactga cgctgaaatt catttgcacc acgggtaaac tgccggtccc gtggccgacc
      181 ctggtgacca cgctgacgta tggtgtgcaa tgtttttctc gttacccgga tcacatgaaa
      241 cagcacgact ttttcaaaag tgccatgccg gaaggttatg ttcaggaacg caccattttc
      301 tttaaagatg acggcaacta caaaacccgt gcagaagtca aatttgaagg tgatacgctg
      361 gtgaaccgca ttgaactgaa aggcattgat ttcaaagaag acggtaatat cctgggccat
      421 aaactggaat ataactacaa tagccacaac gtgtatatta tggccgataa acagaaaaac
      481 ggtatcaaag ttaacttcaa aatccgtcat aatatcgaag atggctctgt ccagctggca
      541 gaccactatc agcaaaacac cccgattggc gatggtccgg tgctgctgcc ggacaatcat
      601 tacctgagca cgcagtctgc gctgagtaaa gatccgaacg aaaaacgcga ccacatggtt
      661 ctgctggagt tcgtcaccgc ggccggtatt acgctgggca tggatgaact gtataaaggt
      721 ggacctggag gatccataat tcacctcgag tactgcatca cgggagatgc actagttgcc
      781 ctacccgagg gcgagtcggt acgcatcgcc gacatcgtgc cgggtgcgcg gcccaacagt
      841 gacaacgcca tcgacctgaa agtccttgac cggcatggca atcccgtgct cgccgaccgg
      901 ctgttccact ccggcgagca tccggtgtac acggtgcgta cggtcgaagg tctgcgtgtg
      961 acgggcaccg cgaaccaccc gttgttgtgt ttggtcgacg tcgccggggt gccgaccctg
     1021 ctgtggaagc tgatcgacga aatcaagccg ggcgattacg cggtgattca acgcagcgca
     1081 ttcagcgtcg actgtgcagg ttttgcccgc gggaaacccg aatttgcgcc cacaacctac
     1141 acagtcggcg tccctggact ggtgcgtttc ttggaagcac accaccgaga cccggacgcc
     1201 caagctatcg ccgacgagct gaccgacggg cggttctact acgcgaaagt cgccagtgtc
     1261 accgacgccg gcgtgcagcc ggtgtatagc cttcgtgtcg acacggcaga ccacgcgttt
     1321 atcacgaacg ggttcgtcag ccacgctact ggcctcaccg gtctgaactc aggcctcacg
     1381 acaaatcctg gtgtatccgc ttggcaggtc aacacagctt atactgcggg acaattggtc
     1441 acatataacg gcaagacgta taaatgtttg cagccccaca cctccttggc aggatgggaa
     1501 ccatccaacg ttcctgcctt gtggcagctt caacatcacc atcaccatca ctaa
//
