LOCUS       sequence-335830-        9546 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses NFAC3 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9546)
  AUTHORS   Goos H, Kinnunen M, Salokas K, Tan Z, Liu X, Yadav L, Zhang Q, Wei 
            GH, Varjosalo M
  TITLE     Human transcription factor protein interaction networks.
  JOURNAL   Nat Commun. 2022 Feb 9;13(1):766. doi: 10.1038/s41467-022-28341-5.
  PUBMED    35140242
REFERENCE   2  (bases 1 to 9546)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-02-9"; volume: "13(1):766. doi"; pages: " 
            10.1038/s41467-022-28341-5"
COMMENT     Sequence Label: MAC_N_NFAC3
FEATURES             Location/Qualifiers
     source          1..9546
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    586..604
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    607..625
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     635..659
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(827..1177,1178..1180,1181..1789)
                     /codon_start=1
                     /product="promiscuous R118G mutant of the E. coli biotin 
                     protein ligase (Cronan, 2005)"
                     /label=BirA(R118G)
                     /note="also known as BirA* or BioID"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRD
                     WGVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSG
                     DACIAEYQQAGRGGRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRK
                     LGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQG
                     WITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIG
                     DKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     CDS             1790..1816
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1847..1870
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             1907..1930
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     protein_bind    1943..1967
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5202..5226)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(5264..5281)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5270..5494
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5627..5646)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    5778..5825
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             5833..6855
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             5833..6855
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    6985..7106
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7022..7041)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7076..7095
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7155..7171)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7155..7171)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7168..7190)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7179..7195
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7203..7209,7210..7227,7228..7233))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7248..7269
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7386..7403)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7557..8145)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7637..7656)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8316..9107,9108..9176))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8939..8958
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9177..9281)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9356..9375)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctccct atcagtgata
      601 gagatctccc tatcagtgat agagatcgtc gacgagctcg tttagtgaac cgtcagatcg
      661 cctggagacg ccatccacgc tgttttgacc tccatagaag acaccgggac cgatccagcc
      721 tccggactct agcgtttaaa cttaagcttg gtaccgagct cggatccact agtccagtgt
      781 ggtggaattc tgcagatatc cagcacagtg gcggccgctc gagaccatga aggacaacac
      841 cgtgcccctg aagctgatcg ccctgctggc caacggcgag ttccactctg gcgagcagct
      901 gggcgagaca ctgggcatga gcagagccgc catcaacaag cacatccaga ccctgcggga
      961 ctggggcgtg gacgtgttca cagtgcccgg caagggctac agcctgcccg agcccatcca
     1021 gctgctgaac gccaagcaga tcctgggcca gctggatggc ggatctgtgg ccgtgctgcc
     1081 cgtgatcgac agcaccaacc agtatctgct cgaccggatc ggcgagctga agtccggcga
     1141 cgcctgtatc gccgagtacc agcaggccgg cagaggcggc aggggcagaa agtggttcag
     1201 ccccttcggc gccaacctgt acctgagcat gttctggaga ctggaacagg gcccagccgc
     1261 cgctattggc ctgagcctgg tcatcggcat cgtgatggcc gaggtgctgc ggaagctggg
     1321 cgccgacaaa gtgcgcgtga agtggcccaa cgacctgtat ctgcaggacc ggaagctggc
     1381 cggcatcctg gtggaactga ccggcaaaac aggcgacgcc gcccagatcg tgatcggcgc
     1441 tggcatcaac atggccatgc ggcgggtgga agagagcgtg gtcaaccagg gctggatcac
     1501 cctgcaggaa gccggcatca acctggaccg gaacaccctg gccgccatgc tgatcagaga
     1561 gctgagagcc gccctggaac tgttcgagca ggaaggcctg gccccctacc tgagcagatg
     1621 ggagaagctg gacaacttca tcaaccggcc cgtgaaactg atcatcggcg acaaagagat
     1681 cttcggcatc agccggggca tcgacaagca gggggccctg ctcctggaac aggacggcat
     1741 catcaagccc tggatgggcg gcgagatcag cctgcggagc gccgagaagt acccatacga
     1801 tgttcctgac tatgccggta ccgagctcgg atccaccatg gctagctgga gccacccgca
     1861 gttcgagaaa ggtggaggtt ccggaggtgg atcgggaggt ggatcgtgga gccacccgca
     1921 gttcgaaaaa gcggccgata tcacaagttt gtacaaaaaa gcaggctcca ccatgactac
     1981 tgcaaactgt ggcgcccacg acgagctcga cttcaaactc gtctttggcg aggacggggc
     2041 gccggcgccg ccgcccccgg gctcgcggcc tgcagatctt gagccagatg attgtgcatc
     2101 catttacatc tttaatgtag atccacctcc atctacttta accacaccac tttgcttacc
     2161 acatcatgga ttaccgtctc actcttctgt tttgtcacca tcgtttcagc tccaaagtca
     2221 caaaaactat gaaggaactt gtgagattcc tgaatctaaa tatagcccat taggtggtcc
     2281 caaacccttt gagtgcccaa gtattcaaat tacatctatc tctcctaact gtcatcaaga
     2341 attagatgca catgaagatg acctacagat aaatgaccca gaacgggaat ttttggaaag
     2401 gccttctaga gatcatctct atcttcctct tgagccatcc taccgggagt cttctcttag
     2461 tcctagtcct gccagcagca tctcttctag gagttggttc tctgatgcat cttcttgtga
     2521 atcgctttca catatttatg atgatgtgga ctcagagttg aatgaagctg cagcccgatt
     2581 tacccttgga tcccctctga cttctcctgg tggctctcca gggggctgcc ctggagaaga
     2641 aacttggcat caacagtatg gacttggaca ctcattatca cccaggcaat ctccttgcca
     2701 ctctcctaga tccagtgtca ctgatgagaa ttggctgagc cccaggccag cctcaggacc
     2761 ctcatcaagg cccacatccc cctgtgggaa acggaggcac tccagtgctg aagtttgtta
     2821 tgctgggtcc ctttcacccc atcactcacc tgttccttca cctggtcact cccccagggg
     2881 aagtgtgaca gaagatacgt ggctcaatgc ttctgtccat ggtgggtcag gccttggccc
     2941 tgcagttttt ccatttcagt actgtgtaga gactgacatc cctctcaaaa caaggaaaac
     3001 ttctgaagat caagctgcca tactaccagg aaaattagag ctgtgttcag atgaccaagg
     3061 gagtttatca ccagcccggg agacttcaat agatgatggc cttggatctc agtatccttt
     3121 aaagaaagat tcatgtggtg atcagtttct ttcagttcct tcacccttta cctggagcaa
     3181 accaaagcct ggccacaccc ctatatttcg cacatcttca ttacctccac tagactggcc
     3241 tttaccagct cattttggac aatgtgaact gaaaatagaa gtgcaaccta aaactcatca
     3301 tcgagcccat tatgaaactg aaggtagccg aggggcagta aaagcatcta ctgggggaca
     3361 tcctgttgtg aagctcctgg gctataacga aaagccaata aatctacaaa tgtttattgg
     3421 gacagcagat gatcgatatt tacgacctca tgcattttac caggtgcatc gaatcactgg
     3481 gaagacagtc gctactgcaa gccaagagat aataattgcc agtacaaaag ttctggaaat
     3541 tccacttctt cctgaaaata atatgtcagc cagtattgat tgtgcaggta ttttgaaact
     3601 ccgcaattca gatatagaac ttcgaaaagg agaaactgat attggcagaa agaatactag
     3661 agtacgactt gtgtttcgtg tacacatccc acagcccagt ggaaaagtcc tttctctgca
     3721 gatagcctct atacccgttg agtgctccca gcggtctgct caagaacttc ctcatattga
     3781 gaagtacagt atcaacagtt gttctgtaaa tggaggtcat gaaatggttg tgactggatc
     3841 taattttctt ccagaatcca aaatcatttt tcttgaaaaa ggacaagatg gacgacctca
     3901 gtgggaggta gaagggaaga taatcaggga aaaatgtcaa ggggctcaca ttgtccttga
     3961 agttcctcca tatcataacc cagcagttac agctgcagtg caggtgcact tttatctttg
     4021 caatggcaag aggaaaaaaa gccagtctca acgttttact tatacaccag ttttgatgaa
     4081 gcaagaacac agagaagaga ttgatttgtc ttcagttcca tctttgcctg tgcctcatcc
     4141 tgctcagacc cagaggcctt cctctgattc agggtgttca catgacagtg tactgtcagg
     4201 acagagaagt ttgatttgct ccatcccaca aacatatgca tccatggtga cctcatccca
     4261 tctgccacag ttgcagtgta gagatgagag tgttagtaaa gaacagcata tgattccttc
     4321 tccaattgta caccagcctt ttcaagtcac accaacacct cctgtggggt cttcctatca
     4381 gcctatgcaa actaatgttg tgtacaatgg accaacttgt cttcctatta atgctgcctc
     4441 tagtcaagaa tttgattcag ttttgtttca gcaggatgca actctttctg gtttagtgaa
     4501 tcttggctgt caaccactgt catccatacc atttcattct tcaaattcag gctcaacagg
     4561 acatctctta gcccatacac ctcattctgt gcataccctg cctcatctgc aatcaatggg
     4621 atatcattgt tcaaatacag gacaaagatc tctttcttct ccagtggctg accagattac
     4681 aggtcagcct tcgtctcagt tacaacctat tacatatggt ccttcacatt cagggtctgc
     4741 tacaacagct tccccagcag cttctcatcc cttggctagt tcaccgcttt ctgggccacc
     4801 atctcctcag cttcagccta tgccttacca atctcctagc tcaggaactg cctcatcacc
     4861 gtctccagcc accagaatgc attctggaca gcactcaact caagcacaaa gtacgggcca
     4921 ggggggtctt tctgcacctt catccttaat atgtcacagt ttgtgtgatc cagcgtcatt
     4981 tccacctgat ggggcaactg tgagcattaa acctgaacca gaagatcgag agcctaactt
     5041 tgcaaccatt ggtctgcagg acatcacttt agatgatgtg aacgagataa ttgggagaga
     5101 catgtcccag atttctgttt cccaaggagc aggggtgagc aggcaggctc ccctcccgag
     5161 tcctgagtcc ctggatttag gaagatctga tgggctcttg gacccagctt tcttgtacaa
     5221 agtggtgacg taagctaggg gcccgtttaa acccgctgat cagcctcgac tgtgccttct
     5281 agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc
     5341 actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt
     5401 cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat
     5461 agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg
     5521 ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     5581 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     5641 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     5701 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     5761 gatggttcac gtacctagaa gttcctattc cgaagttcct attctctaga aagtatagga
     5821 acttccttgg ccaaaaagcc tgaactcacc gcgacgtctg tcgagaagtt tctgatcgaa
     5881 aagttcgaca gcgtctccga cctgatgcag ctctcggagg gcgaagaatc tcgtgctttc
     5941 agcttcgatg taggagggcg tggatatgtc ctgcgggtaa atagctgcgc cgatggtttc
     6001 tacaaagatc gttatgttta tcggcacttt gcatcggccg cgctcccgat tccggaagtg
     6061 cttgacattg gggaattcag cgagagcctg acctattgca tctcccgccg tgcacagggt
     6121 gtcacgttgc aagacctgcc tgaaaccgaa ctgcccgctg ttctgcagcc ggtcgcggag
     6181 gccatggatg cgatcgctgc ggccgatctt agccagacga gcgggttcgg cccattcgga
     6241 ccgcaaggaa tcggtcaata cactacatgg cgtgatttca tatgcgcgat tgctgatccc
     6301 catgtgtatc actggcaaac tgtgatggac gacaccgtca gtgcgtccgt cgcgcaggct
     6361 ctcgatgagc tgatgctttg ggccgaggac tgccccgaag tccggcacct cgtgcacgcg
     6421 gatttcggct ccaacaatgt cctgacggac aatggccgca taacagcggt cattgactgg
     6481 agcgaggcga tgttcgggga ttcccaatac gaggtcgcca acatcttctt ctggaggccg
     6541 tggttggctt gtatggagca gcagacgcgc tacttcgagc ggaggcatcc ggagcttgca
     6601 ggatcgccgc ggctccgggc gtatatgctc cgcattggtc ttgaccaact ctatcagagc
     6661 ttggttgacg gcaatttcga tgatgcagct tgggcgcagg gtcgatgcga cgcaatcgtc
     6721 cgatccggag ccgggactgt cgggcgtaca caaatcgccc gcagaagcgc ggccgtctgg
     6781 accgatggct gtgtagaagt actcgccgat agtggaaacc gacgccccag cactcgtccg
     6841 agggcaaagg aatagcacgt actacgagat ttcgattcca ccgccgcctt ctatgaaagg
     6901 ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc
     6961 atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg ttacaaataa
     7021 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     7081 ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacctc tagctagagc
     7141 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
     7201 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
     7261 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     7321 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     7381 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     7441 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     7501 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     7561 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     7621 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     7681 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     7741 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     7801 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     7861 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     7921 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     7981 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     8041 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     8101 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     8161 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     8221 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     8281 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     8341 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     8401 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     8461 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     8521 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     8581 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     8641 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     8701 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     8761 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     8821 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     8881 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     8941 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     9001 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     9061 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     9121 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     9181 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     9241 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     9301 ccacctgacg tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa
     9361 tctgctctga tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg
     9421 ctgagtagtg cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca
     9481 tgaagaatct gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata
     9541 cgcgtt
//
