LOCUS       sequence-335835-        8596 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  HiLITR protease with MAVS [R537A, R538A] C-terminal tmd targeting 
            information (Mitochondria and ER insertion).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8596)
  AUTHORS   Coukos RW, Yao D, Sanchez ML, Strand ET, Olive ME, Udeshi ND, 
            Weissman JS, Carr SA, Bassik MC, Ting AY
  TITLE     An engineered transcriptional reporter of protein localization 
            identifies regulators of mitochondrial and ER membrane protein 
            trafficking in high-throughput CRISPRi screens.
  JOURNAL   eLife. 2021 Aug 20;10. pii: 69142. doi: 10.7554/eLife.69142.
  PUBMED    34414886
REFERENCE   2  (bases 1 to 8596)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife."; 
            date: "2021-08-20"; volume: "10. pii: 69142. doi"; pages: " 
            10.7554/eLife.69142"
COMMENT     Sequence Label: EGFP-uTEV1-MAVS-mTA2
FEATURES             Location/Qualifiers
     source          1..8596
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    45..63
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    81..99
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    116..134
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    152..170
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    188..206
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    223..241
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    259..277
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     misc_feature    339..456
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(669..713)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    898..1131
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    1624..1749
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             1796..1976
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(1977..2203)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(2231..2251)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(2231..2249)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2270..2286)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2270..2286)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2283..2305)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2294..2310
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2318..2324,2325..2342,2343..2348))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2363..2384
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2501..2518)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2672..3260)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2752..2771)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3431..4222,4223..4291))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4054..4073
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4292..4363)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      4363..4498
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    4538..4659
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4549..4568)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4603..4622
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             4731..4964
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     CDS             5169..5180
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     5578..5598
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(join(5638..5646,5647..5682,5683..5685,5686..5721,
                     5722..5724,5725..5760,5761..5766,5767..6384))
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     promoter        complement(6394..6904)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     complement(6457..6475)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             complement(join(7828..8538,8539..8541,8542..8544))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(7828..8538,8539..8541,8542..8544))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7870..7891)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     8219..8238
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8478..8499
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      8541..8550
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(join(8567..8596,1..285))
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     primer_bind     complement(8570..8594)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 gcttatatag gcctcccacc gtacacgcct acctcgacat acgttctcta tcactgatag
       61 ggagtaaact cgacatacgt tctctatcac tgatagggat aaactcgaca tacgttctct
      121 atcactgata gggagtaaac tcgacatacg ttctctatca ctgataggga gtaaactcga
      181 catacgttct ctatcactga tagggagtaa actcgacatc gttctctatc actgataggg
      241 agtaaactcg acatacgttc tctatcactg atagggagta aactcgacat atcgattcgc
      301 ggccaaagtg gatctctgct gtccctgtaa taaacccgaa aattttgaat ttttgtaatt
      361 tgtttttgta attctttagt ttgtatgtct gttgctatta tgtctactat tctttcccct
      421 gcactgtacc ccccaatccc cccttttctt ttaaaattgt ggatgaatac tgccatttgt
      481 ctcgaggtcg agaattgtcc cctcggggtt gggaggtggg tctgaaacga taatggtgaa
      541 tatccctgcc taactctatt cactatagaa agtacagcaa aaactattct taaacctacc
      601 aagcctccta ctatcattat gaataatttt atataccaca gccaatttgt tatgttaaac
      661 caattccaca aacttgccca tttatctaat tccaataatt cttgttcatt cttttcttgc
      721 tggttttgcg attcttcaat taaggagtgt attaagcttg tgtaattgtt aatttctctg
      781 tcccactcca tccaggtcgt gtgattccaa atctgttcca gagatttatt actccaacta
      841 gcattccaag gcacagcagt ggtgcaaatg agttttccag agcaacccca aatccccagg
      901 agctgttgat cctttaggta tctttccaca gccaggattc ttgcctggag ctgcttgatg
      961 ccccagactg tgagttgcaa cagatgctgt tgcgcctcaa tagccctcag caaattgttc
     1021 tgctgctgca ctataccaga caataattgt ctggcctgta ccgtcagcgt cattgacgct
     1081 gcgcccatag tgcttcctgc tgctcccaag aacccaagga acaaagctcc tattcccact
     1141 gctctttttt ctctctgcac cactcttctc tttgccttgg tgggtgctac tcctaatggt
     1201 tcaattttta ctactttata tttatataat tcacttctcc aattgtccct catatctcct
     1261 cctccaggtc tgaagatcag cggccgcttg ctgtgcggtg gtcttacttt tgttttgctc
     1321 ttcctctatc ttgtctaaag cttccttggt gtcttttatc tctatccttt gatgcacaca
     1381 atagagggtt gctactgtat tatataatga tctaagttct tctgatcctg tctgaaggga
     1441 tggttgtagc tgtcccagta tttgtctaca gccttctgat gtttctaaca ggccaggatt
     1501 aactgcgaat cgttctagct ccctgcttgc ccatactata tgttttaatt tatatttttt
     1561 ctttccccct ggccttaacc gaattttttc ccatcgcgat ctaattctcc cccgcttaat
     1621 actgacgctc tcgcacccat ctctctcctt ctagcctccg ctagtcaaaa tttttggcgt
     1681 actcaccagt cgccgcccct cgcctcttgc cgtgcgcgct tcagcaagcc gagtcctgcg
     1741 tcgagagagc tcctctggtt tccctttcgc tttcaagtcc ctgttcgggc gccactgcta
     1801 gagattttcc acactgacta aaagggtctg agggatctct agttaccaga gtcacacaac
     1861 agacgggcac acactacttg aagcactcaa ggcaagcttt attgaggctt aagcagtggg
     1921 ttccctagtt agccagagag ctcccaggct cagatctggt ctaaccagag agacccgttt
     1981 atgtatcgag ctaggcactt aaatacaata tctctgcaat gcggcaattc agtggttcgt
     2041 ccaatccatg tcagacccgt ctgttgcctt cctaataagg cacgatcgta ccaccttact
     2101 tccaccaatc ggcatgcacg gtgctttttc tctccttgta aggcatgttg ctaactcatc
     2161 gttaccatgt tgcaagacta caagagtatt gcataagact acattaagct tgcagctcca
     2221 gcttttgttc cctttagtga gggttaattg cgcgcttggc gtaatcatgg tcatagctgt
     2281 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     2341 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     2401 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     2461 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     2521 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     2581 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     2641 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     2701 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     2761 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     2821 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     2881 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     2941 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     3001 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     3061 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     3121 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     3181 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     3241 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     3301 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     3361 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     3421 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     3481 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     3541 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     3601 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     3661 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     3721 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     3781 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     3841 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     3901 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     3961 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     4021 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     4081 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     4141 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     4201 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     4261 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     4321 tttatcaggg ttattgtctc atgagcggat acatatttga aaggcctcca aaaaagcctc
     4381 ctcactactt ctggaatagc tcagaggccg aggcggcctc ggcctctgca taaataaaaa
     4441 aaattagtca gccatggggc ggagaatggg cggaactggg cggagttagg ggcgggatgg
     4501 gcggagttag gggcgggata gctagagcca gacatgataa gatacattga tgagtttgga
     4561 caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt
     4621 gctttatttg taaccattat aagctgcaat aaacaagttc ctctcactct ctgatattca
     4681 tttctttgca agttataaat actgaataat aagatgacat gaactactac tgctagagat
     4741 tttccacact gactaaaagg gtctgaggga tctctagtta ccagagtcac acaacagacg
     4801 ggcacacact acttgaagca ctcaaggcaa gctttattga ggcttaagca gtgggttccc
     4861 tagttagcca gagagctccc aggctcagat ctggtctaac cagagagacc cagtacaagc
     4921 aaaaagcaga tcttgtcttc gttgggagtg aattagccct tccagtcccc ccttttcttt
     4981 taaaaagtgg ctaagatcta cagctgcctt gtaagtcatt ggtcttaaag gtacctctag
     5041 tccggacgcc gaggcgaaac aggcggggag gcggcccaaa gggagatccg actcgtctga
     5101 gggcgaaggc gaagacgcgg aagaggccgc agagccggca gcaggccgcg ggaaggaagg
     5161 tccgctggat tgagggccga agggacgtag cagaaggacg tcccgcgcag aatccaggtg
     5221 gcaacacagg cgagcagcca tggaaaggac gtcagcttcc ccgacaacac cacggaattg
     5281 tcagtgccca acagccgagc ccctgtccag cagcgggcaa ggcaggcggc gatgagttcc
     5341 gccgtggcaa tagggagggg gaaagcgaaa gtcccggaaa ggagctgaca ggtggtggca
     5401 atgccccaac cagtgggggt tgcgtcagca aacacagtgc acaccacgcc acgttgcctg
     5461 acaacgggcc acaactcctc ataaagagac agcaaccagg atttatacaa ggaggagaaa
     5521 atgaaagcca tacgggaagc aatagcatga tacaaaggca ttaaagcagc gtatccacat
     5581 agcgtaaaag gagcaacata gttaagaata cgatatcttg gatcgatcct tacttagtta
     5641 cccggggagc atgtcaaggt caaaatcgtc aagagcgtca gcaggcagca tatcaaggtc
     5701 aaagtcgtca agggcatcgg ctgggagcat gtctaagtca aaatcgtcaa gggcgtcggt
     5761 cggcccgccg ctttcgcact ttagctgttt ctccaggcca catatgatta gttccaggcc
     5821 gaaaaggaag gcaggttcgg ctccctgccg gtcgaacagc tcaattgctt gtctcagaag
     5881 tgggggcata gaatcggtgg taggtgtctc tctttcctct tttgctactt gatgctcctg
     5941 ttcctccaat acgcagccca gtgtaaagtg gcccacggcg gacagagcgt acagtgcgtt
     6001 ctccagggag aagccttgct gacacaggaa cgcgagctga ttttccaggg tttcgtactg
     6061 tttctctgtt gggcgggtgc cgagatgcac tttagccccg tcgcgatgtg agaggagagc
     6121 acagcggtat gacttggcgt tgttccgcag aaagtcttgc catgactcgc cttccagggg
     6181 gcagaagtgg gtatgatgcc tgtccagcat ctcgattggc agggcatcga gcagggcccg
     6241 cttgttcttc acgtgccagt acagggtagg ctgctcaact cccagctttt gagcgagttt
     6301 ccttgtcgtc aggccttcga taccgacacc attgagtaat tccagagctc cgtttatgac
     6361 tttgctcttg tccagtctag acatggtgaa ttgctgggga gagaggtcgg tgattcggtc
     6421 aacgagggag ccgactgccg acgtgcgctc cggaggcttg cagaatgcgg aacaccgcgc
     6481 gggcaggaac agggcccaca ctaccgcccc acaccccgcc tcccgcaccg ccccttcccg
     6541 gccgctgctc tcggcgcgcc ctgctgagca gccgctattg gccacagccc atcgcggtcg
     6601 gcgcgctgcc attgctccct ggcgctgtcc gtctgcgagg gtactagtga gacgtgcggc
     6661 ttccgtttgt cacgtccggc acgccgcgaa ccgcaaggaa ccttcccgac ttaggggcgg
     6721 agcaggaagc gtcgccgggg ggcccacaag ggtagcggcg aagatccggg tgacgctgcg
     6781 aacggacgtg aagaatgtgc gagacccagg gtcggcgccg ctgcgtttcc cggaaccacg
     6841 cccagagcag ccgcgtccct gcgcaaaccc agggctgcct tggaaaaggc gcaaccccaa
     6901 ccccggatga attcggccct agtgcagagc cgcccggtac agcaccacca ggagtgtgac
     6961 taccagcacc cctgtcacag ccacctggag ccacagagcc ccaggtgagg gccttccact
     7021 acctgagccg gaactgccgc tcccctctcc actcccaggc ttaccagacc cagaagtaga
     7081 gccgctaccc tcgcctgatc cgggcttgcc agaacctgac gtggaaccga attccaccat
     7141 gaaaacttta tggccccccc acaatactga gtcagcattt aatcgccaac cactaaccca
     7201 ctgctgcgcc tcctgatttg tcaacaattc catgaagttt ttcggcacgc ttgtgaaata
     7261 attgtttgtg ttggtgaaat tcgatgctga gtgtatacca acaatgaacc catctctagt
     7321 tgatactaat ggattgccac actgcccatc cttggtttga atccaatgct tccagaatat
     7381 gccatcagat gaagggaatg tgcaactagt gtctgacacc atgctagaca tgctcttagt
     7441 ttggaagttg gttgtcacaa gacatatgcg ctcttccctt tgtggctctc taaatttcag
     7501 cttttgagga aatggtggga aatccttagg catgcgaata attatcatgt ccctcccatc
     7561 aatgaggtgt tgttgcaaag tcgtggtgtt cttgaccttg aatacaccat gtagtgattg
     7621 gaccaacagt gttccattat ttcttctaaa caagtgcttg tttgtaatga tgaagggacc
     7681 aaatccaata ccatacaacg atgttgtgtg cccatcagat tcattcgtca aatgacaaat
     7741 ggtgctcgat atcgggttgt aatcacgtgg tcccttaaac aagctttctc cgcctgaacc
     7801 ggatccgcct gtgccagatc caaggtcctt gtacagctcg tccatgccga gagtgatccc
     7861 ggcggcggtc acgaactcca gcaggaccat gtgatcgcgc ttctcgttgg ggtctttgct
     7921 cagggcggac tgggtgctca ggtagtggtt gtcgggcagc agcacggggc cgtcgccgat
     7981 gggggtgttc tgctggtagt ggtcggcgag ctgcacgctg ccgtcctcga tgttgtggcg
     8041 gatcttgaag ttcaccttga tgccgttctt ctgcttgtcg gccatgatat agacgttgtg
     8101 gctgttgtag ttgtactcca gcttgtgccc caggatgttg ccgtcctcct tgaagtcgat
     8161 gcccttcagc tcgatgcggt tcaccagggt gtcgccctcg aacttcacct cggcgcgggt
     8221 cttgtagttg ccgtcgtcct tgaagaagat ggtgcgctcc tggacgtagc cttcgggcat
     8281 ggcggacttg aagaagtcgt gctgcttcat gtggtcgggg tagcggctga agcactgcac
     8341 gccgtaggtc agggtggtca cgagggtggg ccagggcacg ggcagcttgc cggtggtgca
     8401 gatgaacttc agggtcagct tgccgtaggt ggcatcgccc tcgccctcgc cggacacgct
     8461 gaacttgtgg ccgtttacgt cgccgtccag ctcgaccagg atgggcacca ccccggtgaa
     8521 cagctcctcg cccttgctca ccatggtggc gctagccaat tctccaggcg atctgacggt
     8581 tcactaaacg agctct
//
