LOCUS       sequence-335837-        9751 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  HiLITR protease with full-length SQS targeting information (ER).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9751)
  AUTHORS   Coukos RW, Yao D, Sanchez ML, Strand ET, Olive ME, Udeshi ND, 
            Weissman JS, Carr SA, Bassik MC, Ting AY
  TITLE     An engineered transcriptional reporter of protein localization 
            identifies regulators of mitochondrial and ER membrane protein 
            trafficking in high-throughput CRISPRi screens.
  JOURNAL   eLife. 2021 Aug 20;10. pii: 69142. doi: 10.7554/eLife.69142.
  PUBMED    34414886
REFERENCE   2  (bases 1 to 9751)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife."; 
            date: "2021-08-20"; volume: "10. pii: 69142. doi"; pages: " 
            10.7554/eLife.69142"
COMMENT     Sequence Label: EGFP-uTEV1-SQSfl
FEATURES             Location/Qualifiers
     source          1..9751
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    30..48
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    65..83
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    101..119
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    137..155
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    172..190
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    208..226
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     273..297
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      317..326
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(323..325,326..328,329..1039)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(323..325,326..328,329..1039)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(368..389)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(629..648)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     976..997
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        3118..3628
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     3547..3565
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             join(3638..4255,4256..4261,4262..4297,4298..4300,4301..4336,
                     4337..4339,4340..4375,4376..4384)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(4424..4444)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4842..4853)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5058..5291
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5363..5484
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5400..5419)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5454..5473
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5524..5659
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        5659..5730
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5731..5799,5800..6591)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5949..5968)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6762..7350
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7251..7270
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7504..7521
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7638..7659
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7674..7679,7680..7697,7698..7704)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7712..7728
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7717..7739
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7736..7752
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7736..7752
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7771..7791
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7773..7791
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7819..8045
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8046..8226
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8273..8398
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8891..9124
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9309..9353
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9566..9683
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        join(9737..9751,1..300)
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    join(9745..9751,1..12)
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 cagtgataga gaacgtatgt cgagtttact ccctatcagt gatagagaac gatgtcgagt
       61 ttactcccta tcagtgatag agaacgtatg tcgagtttac tccctatcag tgatagagaa
      121 cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt atccctatca
      181 gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgaggt
      241 aggcgtgtac ggtgggaggc ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
      301 tggagaattg gctagcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt
      361 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga
      421 gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa
      481 gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag
      541 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta
      601 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt
      661 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga
      721 ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat
      781 catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga
      841 ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc
      901 cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa
      961 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg
     1021 catggacgag ctgtacaagg accttggatc tggcacaggc ggatccggtt caggcggaga
     1081 aagcttgttt aagggaccac gtgattacaa cccgatatcg agcaccattt gtcatttgac
     1141 gaatgaatct gatgggcaca caacatcgtt gtatggtatt ggatttggtc ccttcatcat
     1201 tacaaacaag cacttgttta gaagaaataa tggaacactg ttggtccaat cactacatgg
     1261 tgtattcaag gtcaagaaca ccacgacttt gcaacaacac ctcattgatg ggagggacat
     1321 gataattatt cgcatgccta aggatttccc accatttcct caaaagctga aatttagaga
     1381 gccacaaagg gaagagcgca tatgtcttgt gacaaccaac ttccaaacta agagcatgtc
     1441 tagcatggtg tcagacacta gttgcacatt cccttcatct gatggcatat tctggaagca
     1501 ttggattcaa accaaggatg ggcagtgtgg caatccatta gtatcaacta gagatgggtt
     1561 cattgttggt atacactcag catcgaattt caccaacaca aacaattatt tcacaagcgt
     1621 gccgaaaaac ttcatggaat tgttgacaaa tcaggaggcg cagcagtggg ttagtggttg
     1681 gcgattaaat gctgactcag tattgtgggg gggccataaa gttttcatgg tgggttccac
     1741 gtcaggttct ggcaagcccg gatcaggcga gggtagcggc tctacttctg ggtctggtaa
     1801 gcctgggagt ggagagggga gcacgcgtgg cagttccggc tcaggtagtg gagagttcgt
     1861 gaaatgcctt ggccaccccg aagagttcta caacctggtg cgcttccgga tcgggggcaa
     1921 gcggaaggtg atgcccaaga tggaccagga ctcgctcagc agcagcctga aaacttgcta
     1981 caagtatctc aatcagacca gtcgcagttt cgcagctgtt atccaggcgc tggatgggga
     2041 aatgcgcaat gcagtgtgca tattttatct ggttctccga gctctggaca cactggaaga
     2101 tgacatgacc atcagtgtgg aaaagaaggt cccgctgtta cacaactttc actctttcct
     2161 ttaccaacca gactggcggt tcatggagag caaggagaag gatcgccagg tgctggagga
     2221 cttcccaacg atctcccttg agtttagaaa tctggctgag aaataccaaa cagtgattgc
     2281 cgacatttgc cggagaatgg gcattgggat ggcagagttt ttggataagc atgtgacctc
     2341 tgaacaggag tgggacaagt actgccacta tgttgctggg ctggtcggaa ttggcctttc
     2401 ccgtcttttc tcagcctcag agtttgaaga ccccttagtt ggtgaagata cagaacgtgc
     2461 caactctatg ggcctgtttc tgcagaaaac aaacatcatc cgtgactatc tggaagacca
     2521 gcaaggagga agagagttct ggcctcaaga ggtttggagc aggtatgtta agaagttagg
     2581 ggattttgct aagccggaga atattgactt ggccgtgcag tgcctgaatg aacttataac
     2641 caatgcactg caccacatcc cagatgtcat cacctacctt tcgagactca gaaaccagag
     2701 tgtgtttaac ttctgtgcta ttccacaggt gatggccatt gccactttgg ctgcctgtta
     2761 taataaccag caggtgttca aaggggcagt gaagattcgg aaagggcaag cagtgaccct
     2821 catgatggat gccaccaata tgccagctgt caaagccatc atatatcagt atatggaaga
     2881 gatttatcat agaatccccg actcagaccc atcttctagc aaaacaaggc agatcatctc
     2941 caccatccgg acgcagaatc ttcccaactg tcagctgatt tcccgaagcc actactcccc
     3001 catctacctg tcgtttgtca tgcttttggc tgccctgagc tggcagtacc tgaccactct
     3061 ctcccaggta acagaagact atgttcagac tggagaacac tgaggccgaa ttcatccggg
     3121 gttggggttg cgccttttcc aaggcagccc tgggtttgcg cagggacgcg gctgctctgg
     3181 gcgtggttcc gggaaacgca gcggcgccga ccctgggtct cgcacattct tcacgtccgt
     3241 tcgcagcgtc acccggatct tcgccgctac ccttgtgggc cccccggcga cgcttcctgc
     3301 tccgccccta agtcgggaag gttccttgcg gttcgcggcg tgccggacgt gacaaacgga
     3361 agccgcacgt ctcactagta ccctcgcaga cggacagcgc cagggagcaa tggcagcgcg
     3421 ccgaccgcga tgggctgtgg ccaatagcgg ctgctcagca gggcgcgccg agagcagcgg
     3481 ccgggaaggg gcggtgcggg aggcggggtg tggggcggta gtgtgggccc tgttcctgcc
     3541 cgcgcggtgt tccgcattct gcaagcctcc ggagcgcacg tcggcagtcg gctccctcgt
     3601 tgaccgaatc accgacctct ctccccagca attcaccatg tctagactgg acaagagcaa
     3661 agtcataaac ggagctctgg aattactcaa tggtgtcggt atcgaaggcc tgacgacaag
     3721 gaaactcgct caaaagctgg gagttgagca gcctaccctg tactggcacg tgaagaacaa
     3781 gcgggccctg ctcgatgccc tgccaatcga gatgctggac aggcatcata cccacttctg
     3841 ccccctggaa ggcgagtcat ggcaagactt tctgcggaac aacgccaagt cataccgctg
     3901 tgctctcctc tcacatcgcg acggggctaa agtgcatctc ggcacccgcc caacagagaa
     3961 acagtacgaa accctggaaa atcagctcgc gttcctgtgt cagcaaggct tctccctgga
     4021 gaacgcactg tacgctctgt ccgccgtggg ccactttaca ctgggctgcg tattggagga
     4081 acaggagcat caagtagcaa aagaggaaag agagacacct accaccgatt ctatgccccc
     4141 acttctgaga caagcaattg agctgttcga ccggcaggga gccgaacctg ccttcctttt
     4201 cggcctggaa ctaatcatat gtggcctgga gaaacagcta aagtgcgaaa gcggcgggcc
     4261 gaccgacgcc cttgacgatt ttgacttaga catgctccca gccgatgccc ttgacgactt
     4321 tgaccttgat atgctgcctg ctgacgctct tgacgatttt gaccttgaca tgctccccgg
     4381 gtaactaagt aaggatcgat ccaagatatc gtattcttaa ctatgttgct ccttttacgc
     4441 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca
     4501 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg
     4561 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca
     4621 ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg
     4681 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg
     4741 acaattccgt ggtgttgtcg gggaagctga cgtcctttcc atggctgctc gcctgtgttg
     4801 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg
     4861 accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc
     4921 ctcagacgag tcggatctcc ctttgggccg cctccccgcc tgtttcgcct cggcgtccgg
     4981 actagaggta cctttaagac caatgactta caaggcagct gtagatctta gccacttttt
     5041 aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag atctgctttt
     5101 tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
     5161 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
     5221 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     5281 aatctctagc agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa
     5341 atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt acaaataaag
     5401 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     5461 gtccaaactc atcaatgtat cttatcatgt ctggctctag ctatcccgcc cctaactccg
     5521 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     5581 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga
     5641 ggaggctttt ttggaggcct ttcaaatatg tatccgctca tgagacaata accctgataa
     5701 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     5761 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     5821 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     5881 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     5941 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
     6001 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     6061 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     6121 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     6181 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     6241 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
     6301 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     6361 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     6421 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     6481 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     6541 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     6601 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     6661 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     6721 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     6781 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     6841 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     6901 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     6961 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     7021 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     7081 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     7141 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     7201 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     7261 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     7321 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     7381 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     7441 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     7501 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
     7561 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc
     7621 agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac
     7681 tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga
     7741 aacagctatg accatgatta cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag
     7801 ctggagctgc aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa
     7861 cgatgagtta gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg
     7921 aagtaaggtg gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg
     7981 gacgaaccac tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca
     8041 taaacgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
     8101 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
     8161 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
     8221 ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg
     8281 acgcaggact cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag
     8341 tacgccaaaa attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag
     8401 tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa
     8461 gaaaaaatat aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt
     8521 taatcctggc ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc
     8581 atcccttcag acaggatcag aagaacttag atcattatat aatacagtag caaccctcta
     8641 ttgtgtgcat caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga
     8701 agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag
     8761 gaggagatat gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg
     8821 aaccattagg agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag
     8881 cagtgggaat aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg
     8941 cagcgtcaat gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc
     9001 agaacaattt gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg
     9061 gcatcaagca gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc
     9121 tcctggggat ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg
     9181 ctagttggag taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg
     9241 acagagaaat taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc
     9301 agcaagaaaa gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt
     9361 ggtttaacat aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct
     9421 tggtaggttt aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat
     9481 attcaccatt atcgtttcag acccacctcc caaccccgag gggacaattc tcgacctcga
     9541 gacaaatggc agtattcatc cacaatttta aaagaaaagg ggggattggg gggtacagtg
     9601 caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac
     9661 aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat ccactttggc
     9721 cgcgaatcga tatgtcgagt ttactcccta t
//
