LOCUS       sequence-335838-        8893 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CRISPR sgRNA vector (puro-BFP with BstXI/BlpI digest sites)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8893)
  AUTHORS   Coukos RW, Yao D, Sanchez ML, Strand ET, Olive ME, Udeshi ND, 
            Weissman JS, Carr SA, Bassik MC, Ting AY
  TITLE     An engineered transcriptional reporter of protein localization 
            identifies regulators of mitochondrial and ER membrane protein 
            trafficking in high-throughput CRISPRi screens.
  JOURNAL   eLife. 2021 Aug 20;10. pii: 69142. doi: 10.7554/eLife.69142.
  PUBMED    34414886
REFERENCE   2  (bases 1 to 8893)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife."; 
            date: "2021-08-20"; volume: "10. pii: 69142. doi"; pages: " 
            10.7554/eLife.69142"
COMMENT     Sequence Label: pMCB619
FEATURES             Location/Qualifiers
     source          1..8893
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(147..735)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(227..246)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(906..1697,1698..1766))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1529..1548
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1767..1871)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1946..1965)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        2137..2516
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2518..2716
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2671..2691
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             2734..2914
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2961..3086
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3583..3816
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             4001..4045
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4343..4460
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    complement(4721..4754)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     4758..4778
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     promoter        5031..5242
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          5243..6181
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     6138..6158
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             6202..6798
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6202..6221)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6698..6718
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             6808..6861
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             6874..7572
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="SELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKVV
                     EGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTAT
                     QDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALKL
                     VGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYCD
                     LPSKLGHKLN"
     protein_bind    complement(7592..7625)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    7681..8269
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7734..7754)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8152..8163)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(8272..8288)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8273..8289)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             join(8798..8893,1..85)
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
       61 tagtcagtgt ggaaaatctc tagcagcatg tgagcaaaag gccagcaaaa ggccaggaac
      121 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
      181 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
      241 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
      301 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
      361 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
      421 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
      481 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
      541 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
      601 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
      661 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
      721 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
      781 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
      841 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
      901 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
      961 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     1021 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     1081 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     1141 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     1201 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     1261 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     1321 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     1381 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     1441 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     1501 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     1561 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     1621 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     1681 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     1741 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     1801 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     1861 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc gggagatctc
     1921 ccgatcccct atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt
     1981 atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta
     2041 caacaaggca aggcttgacc gacaattgca tgaagaatct gcttagggtt aggcgttttg
     2101 cgctgcttcg cgatgtacgg gccagatata cgcgttgaca ttgattattg actagttatt
     2161 aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat
     2221 aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa
     2281 taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg
     2341 agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc
     2401 cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct
     2461 tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga
     2521 tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa
     2581 gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc
     2641 caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg
     2701 aggtctatat aagcagcgcg ttttgcctgt actgggtctc tctggttaga ccagatctga
     2761 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
     2821 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
     2881 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
     2941 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
     3001 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
     3061 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
     3121 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
     3181 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
     3241 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     3301 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     3361 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     3421 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     3481 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     3541 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     3601 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     3661 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     3721 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     3781 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     3841 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     3901 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     3961 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     4021 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     4081 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     4141 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     4201 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     4261 cagagacaga tccattcgat tagtgaacgg atcggcactg cgtgcgccaa ttctgcagac
     4321 aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag
     4381 gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa
     4441 ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca gtttggttag
     4501 taccgggccc gccctgcagg tctagagatc cgacgcgcca tctctaggcc cgcgccggcc
     4561 ccctcgcacg gacttgtggg agaagctcgg ctactcccct gccccggtta atttgcatat
     4621 aatatttcct agtaactata gaggcttaat gtgcgataaa agacagataa tctgttcttt
     4681 ttaatactag ctacatttta catgataggc ttggatttct ataacttcgt atagcataca
     4741 ttatacgaag ttataaacag cacaaaagga aactcaccct aactgtaaag taattgtgtg
     4801 ttttgagact ataagtatcc cttggagaac caccttgttg gggcgcgagg tacgtacgtg
     4861 gtttaagagc taagctggaa acagcatagc aagtttaaat aaggctagtc cgttatcaac
     4921 ttgaaaaagt ggcaccgagt cggtgctttt tttctcgagt actaggatcc attaggcggc
     4981 cgccctgcag ggtggataac cgtattaccg ccatgcatgt gcccgtcagt gggcagagcg
     5041 cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa ccggtgccta
     5101 gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc gcctttttcc
     5161 cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa
     5221 cgggtttgcc gccagaacac aggtaagtgc cgtgtgtggt tcccgcgggc ctggcctctt
     5281 tacgggttat ggcccttgcg tgccttgaat tacttccacc tggctgcagt acgtgattct
     5341 tgatcccgag cttcgggttg gaagtgggtg ggagagttcg aggccttgcg cttaaggagc
     5401 cccttcgcct cgtgcttgag ttgaggcctg gcctgggcgc tggggccgcc gcgtgcgaat
     5461 ctggtggcac cttcgcgcct gtctcgctgc tttcgataag tctctagcca tttaaaattt
     5521 ttgatgacct gctgcgacgc tttttttctg gcaagatagt cttgtaaatg cgggccaaga
     5581 tctgcacact ggtatttcgg tttttggggc cgcgggcggc gacggggccc gtgcgtccca
     5641 gcgcacatgt tcggcgaggc ggggcctgcg agcgcggcca ccgagaatcg gacgggggta
     5701 gtctcaagct ggccggcctg ctctggtgcc tggcctcgcg ccgccgtgta tcgccccgcc
     5761 ctgggcggca aggctggccc ggtcggcacc agttgcgtga gcggaaagat ggccgcttcc
     5821 cggccctgct gcagggagct caaaatggag gacgcggcgc tcgggagagc gggcgggtga
     5881 gtcacccaca caaaggaaaa gggcctttcc gtcctcagcc gtcgcttcat gtgactccac
     5941 ggagtaccgg gcgccgtcca ggcacctcga ttagttctcg cgcttttgga gtacgtcgtc
     6001 tttaggttgg ggggaggggt tttatgcgat ggagtttccc cacactgagt gggtggagac
     6061 tgaagttagg ccagcttggc acttgatgta attctccttg gaatttgccc tttttgagtt
     6121 tggatcttgg ttcattctca agcctcagac agtggttcaa agtttttttc ttccatttca
     6181 ggtgtcgtga gctagcccac catgaccgag tacaagccca cggtgcgcct cgccacccgc
     6241 gacgacgtcc cccgggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg
     6301 cgccacaccg tcgacccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc
     6361 ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg
     6421 gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg
     6481 cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg
     6541 gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac
     6601 caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc
     6661 gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg
     6721 ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg
     6781 acccgcaagc ccggtgccgg atcgggagag ggcagaggaa gtctgctaac atgcggtgac
     6841 gtcgaggaga atcctggccc accggtcgcc accagcgagc tgattaagga gaacatgcac
     6901 atgaagctgt acatggaggg caccgtggac aaccatcact tcaagtgcac atccgagggc
     6961 gaaggcaagc cctacgaggg cacccagacc atgagaatca aggtggtcga gggcggccct
     7021 ctccccttcg ccttcgacat cctggctact agcttcctct acggcagcaa gaccttcatc
     7081 aaccacaccc agggcatccc cgacttcttc aagcagtcct tccctgaggg cttcacatgg
     7141 gagagagtca ccacatacga agacgggggc gtgctgaccg ctacccagga caccagcctc
     7201 caggacggct gcctcatcta caacgtcaag atcagagggg tgaacttcac atccaacggc
     7261 cctgtgatgc agaagaaaac actcggctgg gaggccttca ccgagacgct gtaccccgct
     7321 gacggcggcc tggaaggcag aaacgacatg gccctgaagc tcgtgggcgg gagccatctg
     7381 atcgcaaaca tcaagaccac atatagatcc aagaaacccg ctaagaacct caagatgcct
     7441 ggcgtctact atgtggacta cagactggaa agaatcaagg aggccaacaa cgagacctac
     7501 gtcgagcagc acgaggtggc agtggccaga tactgcgacc tccctagcaa actggggcac
     7561 aagcttaatt aagaattcgt cgagggacct aataacttcg tatagcatac attatacgaa
     7621 gttatacatg tttaagggtt ccggttccac taggtacaat tcgatatcaa gcttatcgat
     7681 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
     7741 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
     7801 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
     7861 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
     7921 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
     7981 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
     8041 ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc
     8101 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
     8161 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt
     8221 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgca tcgataccgt
     8281 cgacctcgat cgagacctag aaaaacatgg agcaatcaca agtagcaata cagcagctac
     8341 caatgctgat tgtgcctggc tagaagcaca agaggaggag gaggtgggtt ttccagtcac
     8401 acctcaggta cctttaagac caatgactta caaggcagct gtagatctta gccacttttt
     8461 aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag atatccttga
     8521 tctgtggatc taccacacac aaggctactt ccctgattgg cagaactaca caccagggcc
     8581 agggatcaga tatccactga cctttggatg gtgctacaag ctagtaccag ttgagcaaga
     8641 gaaggtagaa gaagccaatg aaggagagaa cacccgcttg ttacaccctg tgagcctgca
     8701 tgggatggat gacccggaga gagaagtatt agagtggagg tttgacagcc gcctagcatt
     8761 tcatcacatg gcccgagagc tgcatccgga ctgtactggg tctctctggt tagaccagat
     8821 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     8881 gccttgagtg ctt
//
