LOCUS       sequence-33584-e         701 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 701)
  TITLE     Fitzgerald Lab Plasmids
REFERENCE   2  (bases 1 to 701)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pEF-BOS huTBK1 Flag-His
FEATURES             Location/Qualifiers
     source          1..701
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(63..80)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(81..104)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(114..125)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 ggagacctga tactctcaag ggtcccaggt ggggaccctc actctagagt cgcggccgct
       61 cagtggtggt ggtggtggtg cttgtcatcg tcgtccttgt agtccttgac gatccttcct
      121 tcaatggatc caagacagtc aacgttgcga aggccaccat ccatggttaa agagccaaac
      181 ctttctaaaa tgtggttatt ttcagcaagt tctttaacca ccccttccat ctcttccttt
      241 aatttcttca taccaagagt catttctact aatgtgttag aacttggata aattggggtc
      301 atggtatgtt tgattccact ggaagctgta aacattttct gaggcagagt ttcttgtaac
      361 tcattagtat attcttgata ttttgatact tcttcttcaa tatcaaaaca ctgattagtc
      421 agcgataata actgtttcct aagatgaagc atctttctta tccattcttc tgacttattc
      481 aaaaatgcct catacttttt aacacattca tctgtaaagt gcgtcatagc ttttgtggca
      541 tggtaataca gtttttgctt atcaaatttg tggatttgtt cttcattata agctaatcta
      601 cgttctgctt tgtctttttt gaactgatag taaatctctg tcatgcaatt taacaggact
      661 tgtagttttt ctacatttct gtctttcgga tgagtgcctt c
//
