LOCUS       sequence-335842-        8610 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  HiLITR protease with CB5 C-terminal tmd targeting information (ER).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8610)
  AUTHORS   Coukos RW, Yao D, Sanchez ML, Strand ET, Olive ME, Udeshi ND, 
            Weissman JS, Carr SA, Bassik MC, Ting AY
  TITLE     An engineered transcriptional reporter of protein localization 
            identifies regulators of mitochondrial and ER membrane protein 
            trafficking in high-throughput CRISPRi screens.
  JOURNAL   eLife. 2021 Aug 20;10. pii: 69142. doi: 10.7554/eLife.69142.
  PUBMED    34414886
REFERENCE   2  (bases 1 to 8610)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife."; 
            date: "2021-08-20"; volume: "10. pii: 69142. doi"; pages: " 
            10.7554/eLife.69142"
COMMENT     Sequence Label: EGFP-uTEV1-CB5
FEATURES             Location/Qualifiers
     source          1..8610
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    47..65
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    83..101
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    118..136
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    154..172
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    190..208
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    225..243
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    261..279
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     misc_feature    341..458
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(671..715)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    900..1133
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    1626..1751
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             1798..1978
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(1979..2205)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(2233..2253)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(2233..2251)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2272..2288)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2272..2288)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2285..2307)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2296..2312
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2320..2326,2327..2344,2345..2350))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2365..2386
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2503..2520)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2674..3262)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2754..2773)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3433..4224,4225..4293))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4056..4075
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4294..4365)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      4365..4500
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    4540..4661
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4551..4570)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4605..4624
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             4733..4966
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     CDS             5171..5182
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     5580..5600
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(join(5640..5648,5649..5684,5685..5687,5688..5723,
                     5724..5726,5727..5762,5763..5768,5769..6386))
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     promoter        complement(6396..6905)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     complement(6459..6477)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             complement(join(7844..8554,8555..8557,8558..8560))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(7844..8554,8555..8557,8558..8560))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7886..7907)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     8235..8254
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8494..8515
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      8557..8566
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(join(8583..8610,1..287))
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     primer_bind     complement(8586..8610)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 ctgcttatat aggcctccca ccgtacacgc ctacctcgac atacgttctc tatcactgat
       61 agggagtaaa ctcgacatac gttctctatc actgataggg ataaactcga catacgttct
      121 ctatcactga tagggagtaa actcgacata cgttctctat cactgatagg gagtaaactc
      181 gacatacgtt ctctatcact gatagggagt aaactcgaca tcgttctcta tcactgatag
      241 ggagtaaact cgacatacgt tctctatcac tgatagggag taaactcgac atatcgattc
      301 gcggccaaag tggatctctg ctgtccctgt aataaacccg aaaattttga atttttgtaa
      361 tttgtttttg taattcttta gtttgtatgt ctgttgctat tatgtctact attctttccc
      421 ctgcactgta ccccccaatc cccccttttc ttttaaaatt gtggatgaat actgccattt
      481 gtctcgaggt cgagaattgt cccctcgggg ttgggaggtg ggtctgaaac gataatggtg
      541 aatatccctg cctaactcta ttcactatag aaagtacagc aaaaactatt cttaaaccta
      601 ccaagcctcc tactatcatt atgaataatt ttatatacca cagccaattt gttatgttaa
      661 accaattcca caaacttgcc catttatcta attccaataa ttcttgttca ttcttttctt
      721 gctggttttg cgattcttca attaaggagt gtattaagct tgtgtaattg ttaatttctc
      781 tgtcccactc catccaggtc gtgtgattcc aaatctgttc cagagattta ttactccaac
      841 tagcattcca aggcacagca gtggtgcaaa tgagttttcc agagcaaccc caaatcccca
      901 ggagctgttg atcctttagg tatctttcca cagccaggat tcttgcctgg agctgcttga
      961 tgccccagac tgtgagttgc aacagatgct gttgcgcctc aatagccctc agcaaattgt
     1021 tctgctgctg cactatacca gacaataatt gtctggcctg taccgtcagc gtcattgacg
     1081 ctgcgcccat agtgcttcct gctgctccca agaacccaag gaacaaagct cctattccca
     1141 ctgctctttt ttctctctgc accactcttc tctttgcctt ggtgggtgct actcctaatg
     1201 gttcaatttt tactacttta tatttatata attcacttct ccaattgtcc ctcatatctc
     1261 ctcctccagg tctgaagatc agcggccgct tgctgtgcgg tggtcttact tttgttttgc
     1321 tcttcctcta tcttgtctaa agcttccttg gtgtctttta tctctatcct ttgatgcaca
     1381 caatagaggg ttgctactgt attatataat gatctaagtt cttctgatcc tgtctgaagg
     1441 gatggttgta gctgtcccag tatttgtcta cagccttctg atgtttctaa caggccagga
     1501 ttaactgcga atcgttctag ctccctgctt gcccatacta tatgttttaa tttatatttt
     1561 ttctttcccc ctggccttaa ccgaattttt tcccatcgcg atctaattct cccccgctta
     1621 atactgacgc tctcgcaccc atctctctcc ttctagcctc cgctagtcaa aatttttggc
     1681 gtactcacca gtcgccgccc ctcgcctctt gccgtgcgcg cttcagcaag ccgagtcctg
     1741 cgtcgagaga gctcctctgg tttccctttc gctttcaagt ccctgttcgg gcgccactgc
     1801 tagagatttt ccacactgac taaaagggtc tgagggatct ctagttacca gagtcacaca
     1861 acagacgggc acacactact tgaagcactc aaggcaagct ttattgaggc ttaagcagtg
     1921 ggttccctag ttagccagag agctcccagg ctcagatctg gtctaaccag agagacccgt
     1981 ttatgtatcg agctaggcac ttaaatacaa tatctctgca atgcggcaat tcagtggttc
     2041 gtccaatcca tgtcagaccc gtctgttgcc ttcctaataa ggcacgatcg taccacctta
     2101 cttccaccaa tcggcatgca cggtgctttt tctctccttg taaggcatgt tgctaactca
     2161 tcgttaccat gttgcaagac tacaagagta ttgcataaga ctacattaag cttgcagctc
     2221 cagcttttgt tccctttagt gagggttaat tgcgcgcttg gcgtaatcat ggtcatagct
     2281 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
     2341 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
     2401 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
     2461 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
     2521 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     2581 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     2641 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     2701 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     2761 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     2821 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     2881 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     2941 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     3001 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     3061 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     3121 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     3181 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     3241 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     3301 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     3361 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     3421 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     3481 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     3541 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     3601 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     3661 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     3721 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     3781 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     3841 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     3901 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     3961 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     4021 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     4081 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     4141 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     4201 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     4261 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     4321 catttatcag ggttattgtc tcatgagcgg atacatattt gaaaggcctc caaaaaagcc
     4381 tcctcactac ttctggaata gctcagaggc cgaggcggcc tcggcctctg cataaataaa
     4441 aaaaattagt cagccatggg gcggagaatg ggcggaactg ggcggagtta ggggcgggat
     4501 gggcggagtt aggggcggga tagctagagc cagacatgat aagatacatt gatgagtttg
     4561 gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta
     4621 ttgctttatt tgtaaccatt ataagctgca ataaacaagt tcctctcact ctctgatatt
     4681 catttctttg caagttataa atactgaata ataagatgac atgaactact actgctagag
     4741 attttccaca ctgactaaaa gggtctgagg gatctctagt taccagagtc acacaacaga
     4801 cgggcacaca ctacttgaag cactcaaggc aagctttatt gaggcttaag cagtgggttc
     4861 cctagttagc cagagagctc ccaggctcag atctggtcta accagagaga cccagtacaa
     4921 gcaaaaagca gatcttgtct tcgttgggag tgaattagcc cttccagtcc ccccttttct
     4981 tttaaaaagt ggctaagatc tacagctgcc ttgtaagtca ttggtcttaa aggtacctct
     5041 agtccggacg ccgaggcgaa acaggcgggg aggcggccca aagggagatc cgactcgtct
     5101 gagggcgaag gcgaagacgc ggaagaggcc gcagagccgg cagcaggccg cgggaaggaa
     5161 ggtccgctgg attgagggcc gaagggacgt agcagaagga cgtcccgcgc agaatccagg
     5221 tggcaacaca ggcgagcagc catggaaagg acgtcagctt ccccgacaac accacggaat
     5281 tgtcagtgcc caacagccga gcccctgtcc agcagcgggc aaggcaggcg gcgatgagtt
     5341 ccgccgtggc aatagggagg gggaaagcga aagtcccgga aaggagctga caggtggtgg
     5401 caatgcccca accagtgggg gttgcgtcag caaacacagt gcacaccacg ccacgttgcc
     5461 tgacaacggg ccacaactcc tcataaagag acagcaacca ggatttatac aaggaggaga
     5521 aaatgaaagc catacgggaa gcaatagcat gatacaaagg cattaaagca gcgtatccac
     5581 atagcgtaaa aggagcaaca tagttaagaa tacgatatct tggatcgatc cttacttagt
     5641 tacccgggga gcatgtcaag gtcaaaatcg tcaagagcgt cagcaggcag catatcaagg
     5701 tcaaagtcgt caagggcatc ggctgggagc atgtctaagt caaaatcgtc aagggcgtcg
     5761 gtcggcccgc cgctttcgca ctttagctgt ttctccaggc cacatatgat tagttccagg
     5821 ccgaaaagga aggcaggttc ggctccctgc cggtcgaaca gctcaattgc ttgtctcaga
     5881 agtgggggca tagaatcggt ggtaggtgtc tctctttcct cttttgctac ttgatgctcc
     5941 tgttcctcca atacgcagcc cagtgtaaag tggcccacgg cggacagagc gtacagtgcg
     6001 ttctccaggg agaagccttg ctgacacagg aacgcgagct gattttccag ggtttcgtac
     6061 tgtttctctg ttgggcgggt gccgagatgc actttagccc cgtcgcgatg tgagaggaga
     6121 gcacagcggt atgacttggc gttgttccgc agaaagtctt gccatgactc gccttccagg
     6181 gggcagaagt gggtatgatg cctgtccagc atctcgattg gcagggcatc gagcagggcc
     6241 cgcttgttct tcacgtgcca gtacagggta ggctgctcaa ctcccagctt ttgagcgagt
     6301 ttccttgtcg tcaggccttc gataccgaca ccattgagta attccagagc tccgtttatg
     6361 actttgctct tgtccagtct agacatggtg aattgctggg gagagaggtc ggtgattcgg
     6421 tcaacgaggg agccgactgc cgacgtgcgc tccggaggct tgcagaatgc ggaacaccgc
     6481 gcgggcagga acagggccca cactaccgcc ccacaccccg cctcccgcac cgccccttcc
     6541 cggccgctgc tctcggcgcg ccctgctgag cagccgctat tggccacagc ccatcgcggt
     6601 cggcgcgctg ccattgctcc ctggcgctgt ccgtctgcga gggtactagt gagacgtgcg
     6661 gcttccgttt gtcacgtccg gcacgccgcg aaccgcaagg aaccttcccg acttaggggc
     6721 ggagcaggaa gcgtcgccgg ggggcccaca agggtagcgg cgaagatccg ggtgacgctg
     6781 cgaacggacg tgaagaatgt gcgagaccca gggtcggcgc cgctgcgttt cccggaacca
     6841 cgcccagagc agccgcgtcc ctgcgcaaac ccagggctgc cttggaaaag gcgcaacccc
     6901 aacccggatg aattcggcct tactagtcct cggccatgta caggcggtac atcagggcca
     6961 ccaccagggc ggagatggcg gggatcaccc agttggtcca ccaggaggag ttggactcca
     7021 cggtggtgat tccactacct gagccggaac tgccgctccc ctctccactc ccaggcttac
     7081 cagacccaga agtagagccg ctaccctcgc ctgatccggg cttgccagaa cctgacgtgg
     7141 aaccgaattc caccatgaaa actttatggc ccccccacaa tactgagtca gcatttaatc
     7201 gccaaccact aacccactgc tgcgcctcct gatttgtcaa caattccatg aagtttttcg
     7261 gcacgcttgt gaaataattg tttgtgttgg tgaaattcga tgctgagtgt ataccaacaa
     7321 tgaacccatc tctagttgat actaatggat tgccacactg cccatccttg gtttgaatcc
     7381 aatgcttcca gaatatgcca tcagatgaag ggaatgtgca actagtgtct gacaccatgc
     7441 tagacatgct cttagtttgg aagttggttg tcacaagaca tatgcgctct tccctttgtg
     7501 gctctctaaa tttcagcttt tgaggaaatg gtgggaaatc cttaggcatg cgaataatta
     7561 tcatgtccct cccatcaatg aggtgttgtt gcaaagtcgt ggtgttcttg accttgaata
     7621 caccatgtag tgattggacc aacagtgttc cattatttct tctaaacaag tgcttgtttg
     7681 taatgatgaa gggaccaaat ccaataccat acaacgatgt tgtgtgccca tcagattcat
     7741 tcgtcaaatg acaaatggtg ctcgatatcg ggttgtaatc acgtggtccc ttaaacaagc
     7801 tttctccgcc tgaaccggat ccgcctgtgc cagatccaag gtccttgtac agctcgtcca
     7861 tgccgagagt gatcccggcg gcggtcacga actccagcag gaccatgtga tcgcgcttct
     7921 cgttggggtc tttgctcagg gcggactggg tgctcaggta gtggttgtcg ggcagcagca
     7981 cggggccgtc gccgatgggg gtgttctgct ggtagtggtc ggcgagctgc acgctgccgt
     8041 cctcgatgtt gtggcggatc ttgaagttca ccttgatgcc gttcttctgc ttgtcggcca
     8101 tgatatagac gttgtggctg ttgtagttgt actccagctt gtgccccagg atgttgccgt
     8161 cctccttgaa gtcgatgccc ttcagctcga tgcggttcac cagggtgtcg ccctcgaact
     8221 tcacctcggc gcgggtcttg tagttgccgt cgtccttgaa gaagatggtg cgctcctgga
     8281 cgtagccttc gggcatggcg gacttgaaga agtcgtgctg cttcatgtgg tcggggtagc
     8341 ggctgaagca ctgcacgccg taggtcaggg tggtcacgag ggtgggccag ggcacgggca
     8401 gcttgccggt ggtgcagatg aacttcaggg tcagcttgcc gtaggtggca tcgccctcgc
     8461 cctcgccgga cacgctgaac ttgtggccgt ttacgtcgcc gtccagctcg accaggatgg
     8521 gcaccacccc ggtgaacagc tcctcgccct tgctcaccat ggtggcgcta gccaattctc
     8581 caggcgatct gacggttcac taaacgagct
//
