LOCUS       sequence-33585-e         616 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 616)
  AUTHORS   Grashoff C, Hoffman BD, Brenner MD, Zhou R, Parsons M, Yang MT, 
            McLean MA, Sligar SG, Chen CS, Ha T, Schwartz MA
  TITLE     Measuring mechanical tension across vinculin reveals regulation of 
            focal adhesion dynamics.
  JOURNAL   Nature. 2010 Jul 8. 466(7303):263-6.
  PUBMED    20613844
REFERENCE   2  (bases 1 to 616)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2010 Jul 8. 466(7303):263-6."
COMMENT     Sequence Label: Vinculin-venus
FEATURES             Location/Qualifiers
     source          1..616
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(9..260)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(51..72)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     400..419
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 tcgatttact tgtacagctc gtccatgccg agagtgatcc cggcggcggt cacgaactcc
       61 agcaggacca tgtgatcgcg cttctcgttg gggtctttgc tcagtttgga ctggtagctc
      121 aggtagtggt tgtcgggcag cagcacgggg ccgtcgccga tgggggtgtt ctgctggtag
      181 tggtcggcga gctgcacgcc gccgtcctcg atgttgtggc ggatcttgaa gttggccttg
      241 atgccgttct tctgcttgtc ggcggtgata tagacgttgt ggctgttgta gttgtactcc
      301 agcttgtgcc ccaggatgtt gccgtcctcc ttgaagtcga tgcccttcag ctcgatgcgg
      361 ttcaccaggg tgtcgccctc gaacttcacc tcggcgcggg tcttgtagtt gccgtcgtcc
      421 ttgaagaaga tggtgcgctc ctggacgtag ccttcgggca tggcggactt gaagaagtcg
      481 tgctgcttca tgtggtcggg gtagcgggcg aagcactgca ggccgtagcc cagggtggtc
      541 acgagggtgg gccaggcacg ggcagcttgc cggtggtgca gatcagcttc aggtcagctt
      601 gccgtagtgg catcgc
//
