LOCUS       sequence-335851-         633 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of recombinant fusion protein in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 633)
  AUTHORS   Sowa ST, Galera-Prat A, Wazir S, Alanen HI, Maksimainen MM, Lehtio L
  TITLE     A molecular toolbox for ADP-ribosyl binding proteins.
  JOURNAL   Cell Rep Methods. 2021 Dec 20;1(8):100121. doi: 
            10.1016/j.crmeth.2021.100121. Epub 2021 Nov 11.
  PUBMED    34786571
REFERENCE   2  (bases 1 to 633)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep 
            Methods."; date: "2021-12-20"; volume: "1(8):100121. doi"; pages: " 
            10.1016/j.crmeth.2021.100121. Epub 2021 Nov 11"
COMMENT     Sequence Label: PtxS1-c003
FEATURES             Location/Qualifiers
     source          1..633
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..21
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             46..66
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 atgcaccatc atcatcatca ttcttctggt gtagatctgg gtaccgagaa cttgtacttc
       61 caatccatgg acgatcctcc cgccaccgta taccgctatg actcccgccc gccggaggac
      121 gttttccaga acggattcac ggcgtgggga aacaacgaca atgtgctcga ccatctgacc
      181 ggacgttcct gccaggtcgg cagcagcaac agcgctttcg tctccaccag cagcagccgg
      241 cgctataccg aggtctatct cgaacatcgc atgcaggaag cggtcgaggc cgaacgcgcc
      301 ggcaggggca ccggccactt catcggctac atctacgaag tccgcgccga caacaatttc
      361 tacggcgccg ccagctcgta cttcgaatac gtcgacactt atggcgacaa tgccggccgt
      421 atcctcgccg gcgcgctggc cacctacgat agcgattatc tggcacaccg gcgcattccg
      481 cccgaaaaca tccgcagggt aacgcgggtc tatcacaacg gcatcaccgg cgagaccacg
      541 accacggagt attccaacgc tcgctacgtc agccagcaga ctcgcgccaa tcccaacccc
      601 tacacatcgc gaaggtccgt agcgtcgatc taa
//
