LOCUS       sequence-335889-        9527 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CFP(ST)_EPAC full length_Y(UST) biosensor to measure realtime 
            changes in FRET reflecting fluctuations in cAMP levels in living 
            cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9527)
  AUTHORS   Ponsioen B, Zhao J, Riedl J, Zwartkruis F, van der Krogt G, Zaccolo 
            M, Moolenaar WH, Bos JL, Jalink K
  TITLE     Detecting cAMP-induced Epac activation by fluorescence resonance 
            energy transfer: Epac as a novel cAMP indicator.
  JOURNAL   EMBO Rep. 2004 Dec . 5(12):1176-80.
  PUBMED    15550931
REFERENCE   2  (bases 1 to 9527)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO Rep. 
            2004 Dec . 5(12):1176-80."
COMMENT     Sequence Label: Epac-S-H21
FEATURES             Location/Qualifiers
     source          1..9527
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      10..19
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      14..23
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(28..30,31..33,34..744)
                     /codon_start=1
                     /product="enhanced CFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mECFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3424..3426,3427..3429,3430..4140)
                     /codon_start=1
                     /product="enhanced YFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     promoter        complement(4192..4210)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4193..4210)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4230..4247)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4236..4460
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4506..4934
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4593..4612)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4803..4824
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4943..4963)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4948..5277
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5128..5263
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5190..5209
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5344..6138
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5398..5417)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6008..6027
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6312..6433
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6349..6368)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6403..6422
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6482..6498)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6482..6498)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6495..6517)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6506..6522
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6530..6536,6537..6554,6555..6560))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6575..6596
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6713..6730)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6884..7472)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6964..6983)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7643..8434,8435..8503))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8266..8285
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8504..8608)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8683..8702)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        8874..9253
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9254..9457
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     9408..9428
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     9502..9521
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        9502..9520
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 aagcttgccg ccaccatggc ggccgcaatg gtgagcaagg gcgaggagct gttcaccggg
       61 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc
      121 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc
      181 ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctgggg cgtgcagtgc
      241 ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
      301 ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc
      361 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
      421 aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacatcag ccacaacgtc
      481 tatatcaccg ccgacaagca gaagaacggc atcaaggcca acttcaagat ccgccacaac
      541 atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac
      601 ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccaagct gagcaaagac
      661 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact
      721 ctcggcatgg acgagctgta caagtccgga ctcagatctc gatatctgat ggtgttgaga
      781 aggatgcacc ggccccgaag ctgctcctac cagctgctgc tggagcacca gcgtccgagc
      841 tgcatccagg ggctgcgctg gacaccactc accaacagcg aggagtccct ggatttcagc
      901 gagagcctgg agcaggcctc cacagagcgg gtgctcaggg ctgggaggca gctgcatcgg
      961 catctgctgg ccacctgccc aaacctcacc cgagaccgga agtaccacct taggctctat
     1021 cggcagtgct gctctggccg ggagctggtg gatgggatct tggccctggg acttggggtc
     1081 cattcccgga gccaagttgt gggaatctgc caggtgctgc tggatgaagg tgccctctgc
     1141 catgtgaaac acgactgggc cttccaggac cgagatgccc aattctaccg gttccccggg
     1201 cccgagcccg agcccgtggg aactcatgag atggaggagg agttggccga agctgtggcc
     1261 ctgctctccc agcgggggcc tgacgccctg ctcactgtgg cacttcgaaa gcccccaggt
     1321 cagcgcacgg atgaagagct ggacctcatc tttgaggagc tgctgcacat caaggctgtg
     1381 gcccacctct ccaactcggt gaagcgagaa ttagcggctg ttctgctctt tgaaccacac
     1441 agcaaggcag ggaccgtgtt gttcagccag ggggacaagg gcacttcgtg gtacattatc
     1501 tggaagggat ctgtcaacgt ggtgacccat ggcaaggggc tggtgaccac cctgcatgag
     1561 ggagatgatt ttggacagct ggctctggtg aatgatgcac cccgggcagc caccatcatc
     1621 ctgcgagaag acaactgtca tttcctgcgt gtggacaagc aggacttcaa ccgtatcatc
     1681 aaggatgtgg aggcaaagac catgcggctg gaagaacatg gcaaagtggt gctggtgctg
     1741 gagagagcct ctcagggcgc cggcccttcc cgacccccaa ccccaggcag gaaccggtat
     1801 acagtgatgt ctggcacccc agagaagatc ctagagcttc tgttggaggc catgggacca
     1861 gattccagtg ctcatgaccc aacagagaca ttcctcagcg acttcctcct gacccacagg
     1921 gtcttcatgc ccagcgccca actctgcgct gcccttctgc accacttcca tgtggagcct
     1981 gcgggtggca gcgagcagga gcgcagcacc tacgtctgca acaagaggca gcagatcttg
     2041 cggctggtca gccagtgggt ggccctgtat ggctccatgc tccacactga ccctgtggcc
     2101 accagcttcc tccagaaact ctcagacctg gtgggcaggg acacccgact cagcaacctg
     2161 ctgagggagc agtggccaga gaggcggcga tgccacaggt tggagaatgg ctgtgggaat
     2221 gcatctcctc agatgaaggc ccggaacttg cctgtttggc tccccaacca ggacgagccc
     2281 cttcctggca gcagctgtgc catccaagtt ggggataaag tcccctatga catctgccgg
     2341 ccagaccact cagtgttgac cctgcagctg cctgtgacag cctccgtgag agaggtgatg
     2401 gcagcgttgg cccaggagga tggctggacc aaggggcagg tgctggtgaa ggtcaattct
     2461 gcaggtgatg ccattggcct gcagccagat gcccgtggtg tggccacatc tctggggctc
     2521 aatgagcgtc tctttgttgt caacccacag gaagcgcatg agctgatccc acaccctgac
     2581 cagctggggc ccactgtggg ctctgctgag gggctggacc tggtgagtgc caaggacctg
     2641 gcaggccagc tgacggacca cgactggagc ctcttcaaca gtatccacca ggtggagctg
     2701 atccactatg tgctgggccc ccagcatctg cgggatgtca ccaccgccaa cctggagcgc
     2761 ttcatgcgcc gcttcaatga gctgcagtac tgggtggcca ccgagctgtg tctctgcccc
     2821 gtgcccggcc cccgggccca gctgctcagg aagttcatta agctggcggc ccacctcaag
     2881 gagcagaaga atctcaattc cttctttgcc gtcatgtttg gcctcagcaa ctcggccatc
     2941 agccgcctag cccacacctg ggagcggctg ccccacaaag tccggaagct gtactccgcc
     3001 ctcgagaggc tgctggatcc ctcatggaac caccgggtat accgactggc cctcgccaag
     3061 ctctcccctc ctgtcatccc cttcatgccc cttcttctca aagacatgac cttcattcat
     3121 gagggaaacc acacactagt ggagaatctc atcaactttg agaagatgag aatgatggcc
     3181 agagccgcgc ggatgctgca ccactgccga agccacaacc ctgtgcctct ctcaccactc
     3241 agaagccgag tttcccacct ccacgaggac agccaggtgg cgaggatttc cacatgctcg
     3301 gagcagtccc tgagcacccg gagtccagcc agcacctggg cttatgtcca gcagctgaag
     3361 gtcattgaca accagcggga actctcccgc ctctcccgag agctggagcc agctagcgag
     3421 ctcatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg
     3481 gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc
     3541 tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc
     3601 accctcgtga ccaccttcgg ctacggcgtg cagtgcttcg cccgctaccc cgaccacatg
     3661 aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc
     3721 ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc
     3781 ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg
     3841 cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga caagcagaag
     3901 aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc
     3961 gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac
     4021 cactacctga gctaccagtc caaactgagc aaagacccca acgagaagcg cgatcacatg
     4081 gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag
     4141 ccgaattccc tcgaggttaa cgcggcgcgc ctctaatcta gagggcccta ttctatagtg
     4201 tcacctaaat gctagagctc gctgatcagc ctcgactgtg ccttctagtt gccagccatc
     4261 tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct
     4321 ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg
     4381 gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg
     4441 ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta
     4501 tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
     4561 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
     4621 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
     4681 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
     4741 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
     4801 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
     4861 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
     4921 atttaacgcg aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc
     4981 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga
     5041 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     5101 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     5161 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc
     5221 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag
     5281 ctcccgggag cttgtatatc cattttcgga tctgatcaag agacaggatg aggatcgttt
     5341 cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta
     5401 ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg
     5461 tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa
     5521 ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct
     5581 gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg
     5641 caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca
     5701 atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat
     5761 cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac
     5821 gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc
     5881 gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa
     5941 aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag
     6001 gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc
     6061 ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt
     6121 cttgacgagt tcttctgagc gggactctgg ggttcgaaat gaccgaccaa gcgacgccca
     6181 acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa
     6241 tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct
     6301 tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     6361 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     6421 tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat
     6481 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     6541 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     6601 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     6661 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     6721 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     6781 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     6841 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     6901 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     6961 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     7021 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     7081 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     7141 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     7201 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     7261 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     7321 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     7381 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     7441 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     7501 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     7561 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     7621 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     7681 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     7741 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     7801 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     7861 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     7921 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     7981 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     8041 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     8101 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     8161 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     8221 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     8281 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     8341 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     8401 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     8461 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     8521 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     8581 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtcg
     8641 acggatcggg agatctcccg atcccctatg gtgcactctc agtacaatct gctctgatgc
     8701 cgcatagtta agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc
     8761 gagcaaaatt taagctacaa caaggcaagg cttgaccgac aattgcatga agaatctgct
     8821 tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc gttgacattg
     8881 attattgact agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat
     8941 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
     9001 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
     9061 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
     9121 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
     9181 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
     9241 cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga
     9301 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
     9361 aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg
     9421 taggcgtgta cggtgggagg tctatataag cagagctctc tggctaacta gagaacccac
     9481 tgcttactgg cttatcgaaa ttaatacgac tcactatagg gagaccc
//
